FastQCFastQC Report
Thu 26 May 2016
SRR1660750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29120312
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17272175.931313510651946No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT355790.12217932280395898No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324290.11136213101013478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2170700.086.157918
GTGGTAT2198000.085.472646
AGTGGTA2214950.084.8276755
TGGTATC2207600.084.790167
GTATCAA2213950.084.338969
AAGCAGT2214250.083.143091
GCAGTGG2264200.083.02823
AGCAGTG2329750.080.55092
CAGTGGT2330050.080.488084
CAACGCA2142200.044.6283112-13
ATCAACG2153750.044.4413110-11
ACGCAGA2168250.044.2126714-15
TCAACGC2136150.043.66356312-13
AGAGTAC2190950.043.56958418-19
AACGCAG2154800.043.37399714-15
GAGTACT2139350.043.2321520-21
CGCAGAG2178650.042.95958316-17
TATCAAC2198550.042.43707310-11
AGTACTT2260750.041.93664620-21
GTACTTT2237400.041.34291522-23
Produced by FastQC (version 0.11.5)