##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660750_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29120312 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.007059780128728 33.0 31.0 34.0 27.0 34.0 2 31.79634345950689 34.0 31.0 34.0 28.0 34.0 3 32.30714876955989 34.0 31.0 34.0 30.0 34.0 4 35.83749768889839 37.0 35.0 37.0 35.0 37.0 5 35.89569490189528 37.0 35.0 37.0 35.0 37.0 6 35.925306844239856 37.0 35.0 37.0 35.0 37.0 7 35.90669794334621 37.0 35.0 37.0 35.0 37.0 8 35.922205091758634 37.0 35.0 37.0 35.0 37.0 9 37.63511572266121 39.0 37.0 39.0 35.0 39.0 10-11 37.567545773548034 39.0 37.0 39.0 35.0 39.0 12-13 37.64529075100569 39.0 37.0 39.0 35.0 39.0 14-15 38.97839405017364 40.0 38.0 41.0 35.0 41.0 16-17 38.99759873795308 40.0 38.0 41.0 35.0 41.0 18-19 38.99422427204763 40.0 38.5 41.0 35.0 41.0 20-21 38.929512568409294 40.0 38.5 41.0 35.0 41.0 22-23 38.81726042289657 40.0 38.0 41.0 34.5 41.0 24-25 38.77693042917947 40.0 38.0 41.0 34.5 41.0 26-27 38.75394422285036 40.0 38.0 41.0 34.5 41.0 28-29 38.66511440536763 40.0 38.0 41.0 34.0 41.0 30-31 38.57389338754338 40.0 38.0 41.0 34.0 41.0 32-33 38.475574231484885 40.0 38.0 41.0 34.0 41.0 34-35 38.37065511179963 40.0 38.0 41.0 34.0 41.0 36-37 38.182967287575764 40.0 38.0 41.0 33.5 41.0 38-39 37.739245496408145 40.0 36.0 41.0 33.0 41.0 40-41 37.5974699378221 40.0 36.0 41.0 33.0 41.0 42-43 37.440286113692736 40.0 35.5 41.0 33.0 41.0 44-45 37.325850921514856 39.5 35.0 41.0 32.5 41.0 46-47 37.196520937000955 39.0 35.0 41.0 32.0 41.0 48-49 37.31429273491301 39.5 35.0 41.0 32.0 41.0 50-51 37.218553118524284 39.5 35.0 41.0 32.0 41.0 52-53 36.944944975177464 39.0 35.0 41.0 31.0 41.0 54-55 36.40255461548627 39.0 35.0 41.0 29.0 41.0 56-57 36.04474416345539 39.0 35.0 41.0 27.5 41.0 58-59 35.842371022673106 39.0 35.0 41.0 27.0 41.0 60-61 35.659209832641906 39.0 35.0 41.0 26.5 41.0 62-63 35.38587332100013 38.5 35.0 41.0 26.0 41.0 64-65 35.155160614350564 38.0 35.0 40.0 26.0 41.0 66-67 34.90647837152294 37.0 34.0 40.0 26.0 41.0 68-69 34.63536319597125 37.0 34.0 40.0 26.0 41.0 70-71 34.32438356086295 36.0 34.0 39.0 25.5 41.0 72-73 34.01664767534084 36.0 34.0 39.0 25.5 41.0 74-75 33.65930615715931 35.0 33.5 38.5 25.0 40.0 76-77 31.563727442205977 34.0 30.5 36.0 21.0 39.0 78-79 32.82389294111958 35.0 33.0 37.0 24.0 39.0 80-81 32.80030991769594 35.0 33.0 36.5 24.0 39.0 82-83 32.57075071860494 35.0 33.0 36.0 24.0 38.0 84-85 32.328521445786706 35.0 33.0 36.0 24.0 37.0 86-87 32.1185238846342 35.0 33.0 35.0 23.0 37.0 88-89 31.89689334372516 35.0 33.0 35.0 22.0 36.5 90-91 31.732223490599964 35.0 33.0 35.0 21.0 36.0 92-93 31.600983705119646 35.0 33.0 35.0 20.0 36.0 94-95 31.497949764411864 35.0 33.0 35.0 20.0 36.0 96-97 31.390148343877634 35.0 33.0 35.0 20.0 35.0 98-99 31.271783231580763 35.0 32.0 35.0 19.0 35.0 100 31.086163259514528 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7614504855383721 1101 2 1.2196645625371652 1101 3 0.7805828050880663 1101 4 0.6000204215259259 1101 5 0.4698107731308738 1101 6 0.42979279290648265 1101 7 0.40512384421413117 1101 8 0.40108346555977903 1101 9 0.494679550484193 1101 10-11 0.5162375871498739 1101 12-13 0.4830393854279649 1101 14-15 0.635059829324355 1101 16-17 0.6096575569759679 1101 18-19 0.5616638226565556 1101 20-21 0.5836592096230859 1101 22-23 0.5564899313828136 1101 24-25 0.6131261578344152 1101 26-27 0.628796164583882 1101 28-29 0.6195287262556164 1101 30-31 0.5802035407041686 1101 32-33 0.5828578610446655 1101 34-35 0.597163885151268 1101 36-37 0.5870861519187045 1101 38-39 0.7137243327872298 1101 40-41 0.6679766014127537 1101 42-43 0.6337255626838925 1101 44-45 0.6194653103240881 1101 46-47 0.5820102405184286 1101 48-49 0.5745976558871604 1101 50-51 0.5768939376173066 1101 52-53 0.5772835076346752 1101 54-55 0.6421590389124106 1101 56-57 0.7082856269145523 1101 58-59 0.6754022656684384 1101 60-61 0.639789351000978 1101 62-63 0.6956545087236847 1101 64-65 0.6824709259538864 1101 66-67 0.676166993662008 1101 68-69 0.6767095632414524 1101 70-71 0.6457146022282387 1101 72-73 0.6223165534194592 1101 74-75 0.556745744352753 1101 76-77 0.47211553116171956 1101 78-79 0.6027232651475956 1101 80-81 0.6002356750582223 1101 82-83 0.5360942793963588 1101 84-85 0.5503831945356765 1101 86-87 0.536392555282255 1101 88-89 0.5378666193265396 1101 90-91 0.5492944369865604 1101 92-93 0.46373712721721816 1101 94-95 0.3786254740975359 1101 96-97 0.4151140735603285 1101 98-99 0.38415047168885863 1101 100 0.3178264129171353 1102 1 1.6654005144206074 1102 2 1.1445174039598633 1102 3 0.7034767586706039 1102 4 0.5443010124260894 1102 5 0.43200553444373924 1102 6 0.3773414378861588 1102 7 0.32356334754692284 1102 8 0.3631377142658039 1102 9 0.4366689082114803 1102 10-11 0.4457440046623802 1102 12-13 0.3588465329734305 1102 14-15 0.5175445054089778 1102 16-17 0.5097557073305339 1102 18-19 0.4823155728548443 1102 20-21 0.49176505474557786 1102 22-23 0.5195033094468471 1102 24-25 0.5325977007698981 1102 26-27 0.5015868332922864 1102 28-29 0.506147706192337 1102 30-31 0.5094059135105553 1102 32-33 0.4921507526575013 1102 34-35 0.4697361349114786 1102 36-37 0.5375132808202849 1102 38-39 0.602883414537132 1102 40-41 0.5678348279539378 1102 42-43 0.5789643969018314 1102 44-45 0.541830249405777 1102 46-47 0.5326439229520616 1102 48-49 0.5267709652966559 1102 50-51 0.5170269959175116 1102 52-53 0.4536713534108827 1102 54-55 0.5087293825292463 1102 56-57 0.5778140568093946 1102 58-59 0.5484827221940805 1102 60-61 0.5231886588343713 1102 62-63 0.5792872513795757 1102 64-65 0.5335365000061145 1102 66-67 0.49706867792217935 1102 68-69 0.4800158752008272 1102 70-71 0.4949908355825343 1102 72-73 0.4331282731331427 1102 74-75 0.3994457712121431 1102 76-77 0.32406203901368613 1102 78-79 0.4390007859083198 1102 80-81 0.4105729469752504 1102 82-83 0.3862226025972504 1102 84-85 0.40209035450041597 1102 86-87 0.40291716166941427 1102 88-89 0.41520515618134723 1102 90-91 0.40658126062139743 1102 92-93 0.3378398852618538 1102 94-95 0.3153337277702839 1102 96-97 0.28792973612630135 1102 98-99 0.32006188012356773 1102 100 0.23523299729409075 1103 1 1.6419581947787343 1103 2 1.0749705682585748 1103 3 0.660023342232499 1103 4 0.5200397279200999 1103 5 0.389534499567155 1103 6 0.3552488850391171 1103 7 0.32139252212292746 1103 8 0.34895316616940164 1103 9 0.4237921073754194 1103 10-11 0.37187621405334426 1103 12-13 0.36027234417345966 1103 14-15 0.5330715737492753 1103 16-17 0.47069155706407173 1103 18-19 0.4026437807485621 1103 20-21 0.4121211361836217 1103 22-23 0.43990826627153723 1103 24-25 0.4870749581703677 1103 26-27 0.4775995958442465 1103 28-29 0.47671424350944136 1103 30-31 0.4971174463868522 1103 32-33 0.4813471596108627 1103 34-35 0.4745646281341749 1103 36-37 0.5025947861324269 1103 38-39 0.571661069749787 1103 40-41 0.5260031633032654 1103 42-43 0.4781657856212149 1103 44-45 0.5049535328275141 1103 46-47 0.5022558183207408 1103 48-49 0.4873344450193997 1103 50-51 0.422345186072711 1103 52-53 0.4295993010437371 1103 54-55 0.48711639380628213 1103 56-57 0.49609818338857536 1103 58-59 0.4455083102218964 1103 60-61 0.45819865073763566 1103 62-63 0.4830988650520638 1103 64-65 0.4611707972025769 1103 66-67 0.378124405135118 1103 68-69 0.4061527024452758 1103 70-71 0.4411579989623391 1103 72-73 0.4140020892524561 1103 74-75 0.3607841413315924 1103 76-77 0.1949626982786441 1103 78-79 0.32338960558972474 1103 80-81 0.34211307647866107 1103 82-83 0.32881453457401477 1103 84-85 0.3239658555537659 1103 86-87 0.2972656579086532 1103 88-89 0.36464662414744353 1103 90-91 0.3126412348013581 1103 92-93 0.29165168279259035 1103 94-95 0.27067594154150143 1103 96-97 0.26369908066087433 1103 98-99 0.32691458370521076 1103 100 0.22645544447145838 1104 1 1.5937926029642817 1104 2 1.0818503319102 1104 3 0.6314673787944329 1104 4 0.5237468795228821 1104 5 0.37753434677541264 1104 6 0.34377981788265544 1104 7 0.2906721316885168 1104 8 0.3115875609162799 1104 9 0.3617246755284711 1104 10-11 0.3779532659320566 1104 12-13 0.34882488887028984 1104 14-15 0.49800533877346 1104 16-17 0.4629178696887948 1104 18-19 0.404523944450645 1104 20-21 0.40441916994134885 1104 22-23 0.3931864028083538 1104 24-25 0.45575667541746157 1104 26-27 0.4228405148712895 1104 28-29 0.4320401693820983 1104 30-31 0.46307110742967694 1104 32-33 0.46357017127193245 1104 34-35 0.4122653955980198 1104 36-37 0.44821344838371147 1104 38-39 0.5039218411131259 1104 40-41 0.5327847151415028 1104 42-43 0.5077283481590129 1104 44-45 0.49945677119537635 1104 46-47 0.5558804985243526 1104 48-49 0.5212899126680028 1104 50-51 0.48036702162179523 1104 52-53 0.49529599024154436 1104 54-55 0.549326463439705 1104 56-57 0.5894270062930147 1104 58-59 0.5521987963109254 1104 60-61 0.5417421094539279 1104 62-63 0.5912506058660227 1104 64-65 0.5620243611371549 1104 66-67 0.5513528545213475 1104 68-69 0.5693247923245437 1104 70-71 0.5389019592597535 1104 72-73 0.4974114767020552 1104 74-75 0.46809113186333207 1104 76-77 0.424840390447379 1104 78-79 0.4905596745926175 1104 80-81 0.4270582918294821 1104 82-83 0.4108728409214706 1104 84-85 0.41743780605695946 1104 86-87 0.4305988494932862 1104 88-89 0.44651786302464913 1104 90-91 0.4812337245795497 1104 92-93 0.4712892263125035 1104 94-95 0.43373041116717204 1104 96-97 0.4323198993997508 1104 98-99 0.45848381583860487 1104 100 0.3580974901676619 1105 1 1.563403510070163 1105 2 1.0244071462624298 1105 3 0.5997093268349971 1105 4 0.4881502399607811 1105 5 0.332979189143785 1105 6 0.2743784804583669 1105 7 0.22223510003810532 1105 8 0.2556936865421804 1105 9 0.3189904864098452 1105 10-11 0.30215975629493386 1105 12-13 0.2741338760358971 1105 14-15 0.3918509477952057 1105 16-17 0.3272313861401983 1105 18-19 0.3019768012836579 1105 20-21 0.3281198430065544 1105 22-23 0.2778652744175787 1105 24-25 0.32855639210877285 1105 26-27 0.2853783107703052 1105 28-29 0.3065015590498703 1105 30-31 0.31395016863710623 1105 32-33 0.3313541050949027 1105 34-35 0.31654814619702876 1105 36-37 0.36886955686488676 1105 38-39 0.4091163026173277 1105 40-41 0.39883185110235786 1105 42-43 0.40463938640970554 1105 44-45 0.39666046868762805 1105 46-47 0.42198619722183395 1105 48-49 0.38067494878549013 1105 50-51 0.3890325927510929 1105 52-53 0.36176863183809616 1105 54-55 0.4318401600314772 1105 56-57 0.4534687903344192 1105 58-59 0.4220194890432367 1105 60-61 0.39944350157444575 1105 62-63 0.39410160870462363 1105 64-65 0.37901199734529456 1105 66-67 0.39211644038842763 1105 68-69 0.4389671733905516 1105 70-71 0.4173273314606689 1105 72-73 0.3200550013804886 1105 74-75 0.27971082495828625 1105 76-77 0.2653781383328315 1105 78-79 0.4040152124764589 1105 80-81 0.3885646121761255 1105 82-83 0.3409654893890277 1105 84-85 0.3696707969790012 1105 86-87 0.354369926042601 1105 88-89 0.4123214063746623 1105 90-91 0.40949765512124614 1105 92-93 0.37130012947671176 1105 94-95 0.3416247763100877 1105 96-97 0.35897574682001476 1105 98-99 0.31652867642424454 1105 100 0.3130185473977818 1106 1 1.437857419248754 1106 2 0.8838546005992178 1106 3 0.49578304876457935 1106 4 0.44227630339614166 1106 5 0.2753557044858965 1106 6 0.24849053695422185 1106 7 0.18021883761277735 1106 8 0.21193832941163748 1106 9 0.25124596230405416 1106 10-11 0.24137129948228875 1106 12-13 0.21489959898753597 1106 14-15 0.3412104832310021 1106 16-17 0.25512032871877466 1106 18-19 0.20231342771203487 1106 20-21 0.22598448064461252 1106 22-23 0.19978176707570583 1106 24-25 0.24104055334230168 1106 26-27 0.23142719087650931 1106 28-29 0.24837278899132542 1106 30-31 0.26127103463944934 1106 32-33 0.26109049372965387 1106 34-35 0.26034148458624173 1106 36-37 0.26380745061064914 1106 38-39 0.31747537268346804 1106 40-41 0.30645949427845665 1106 42-43 0.24847907836506522 1106 44-45 0.258095800877868 1106 46-47 0.3004364643901454 1106 48-49 0.27835686536633375 1106 50-51 0.26095577571824435 1106 52-53 0.1998869231341729 1106 54-55 0.2346402415261295 1106 56-57 0.24562095547154428 1106 58-59 0.19439895397503903 1106 60-61 0.1883799588759345 1106 62-63 0.21044284740140284 1106 64-65 0.18547432649008755 1106 66-67 0.18062111712924178 1106 68-69 0.19892782442470036 1106 70-71 0.22236237625654098 1106 72-73 0.19567080218202904 1106 74-75 0.16979180684039363 1106 76-77 0.10969344958237315 1106 78-79 0.21918694345122702 1106 80-81 0.19621808577363709 1106 82-83 0.2033772197995276 1106 84-85 0.21534755659528315 1106 86-87 0.18940957102260114 1106 88-89 0.22981504389202811 1106 90-91 0.23756448442464517 1106 92-93 0.20163819244985248 1106 94-95 0.16957391031515812 1106 96-97 0.17263376334588187 1106 98-99 0.2305520992639707 1106 100 0.16141184883327142 1107 1 1.5871048446055305 1107 2 1.0363279471667646 1107 3 0.596757430045642 1107 4 0.49592456814524866 1107 5 0.32452224936427854 1107 6 0.3013441362925562 1107 7 0.24917265013900902 1107 8 0.268597429142595 1107 9 0.33352613158371014 1107 10-11 0.31083318996554254 1107 12-13 0.2929339956823114 1107 14-15 0.42556009612779633 1107 16-17 0.4002450955070742 1107 18-19 0.3125231983932224 1107 20-21 0.31798439571658577 1107 22-23 0.3363258438464811 1107 24-25 0.38592017052455674 1107 26-27 0.3299025743296369 1107 28-29 0.30294182369861034 1107 30-31 0.3522831426543931 1107 32-33 0.379880273719273 1107 34-35 0.3978735761960408 1107 36-37 0.43034721347036253 1107 38-39 0.45151527874703845 1107 40-41 0.43221649378576643 1107 42-43 0.4214472425234206 1107 44-45 0.38154290201519814 1107 46-47 0.4651288069757058 1107 48-49 0.4457256120831872 1107 50-51 0.4406558826196587 1107 52-53 0.3887643233361331 1107 54-55 0.4801411610239015 1107 56-57 0.44129266870641004 1107 58-59 0.42950615938639203 1107 60-61 0.388593693374645 1107 62-63 0.4276897094573755 1107 64-65 0.4130562928142325 1107 66-67 0.3977755149245681 1107 68-69 0.375328041001346 1107 70-71 0.4150970183910374 1107 72-73 0.3814140876700307 1107 74-75 0.3780914539964897 1107 76-77 0.29565081808194904 1107 78-79 0.38406095123357886 1107 80-81 0.37129855910131937 1107 82-83 0.36877936861101546 1107 84-85 0.38567945551393024 1107 86-87 0.3464601457914398 1107 88-89 0.4139353225791247 1107 90-91 0.3806404736181115 1107 92-93 0.383836399764963 1107 94-95 0.3714377703514167 1107 96-97 0.34948398345049014 1107 98-99 0.3964431208578105 1107 100 0.3249238902814895 1108 1 1.4029118209942872 1108 2 0.8897842107552343 1108 3 0.4527408656686944 1108 4 0.3788793234314056 1108 5 0.18589218021726595 1108 6 0.14839697836927712 1108 7 0.10540126538408856 1108 8 0.12862807185393876 1108 9 0.14295083351409943 1108 10-11 0.0995374807628977 1108 12-13 0.11591769246876993 1108 14-15 0.16111877337063163 1108 16-17 0.09649394247885823 1108 18-19 0.09867260964844604 1108 20-21 0.09690904328120808 1108 22-23 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7543.0 16 16819.0 17 26748.0 18 36074.0 19 48535.0 20 70593.0 21 107130.0 22 166452.0 23 271139.0 24 439226.0 25 555688.0 26 541064.0 27 452264.0 28 409397.0 29 440594.0 30 514102.0 31 623763.0 32 767697.0 33 968839.0 34 1291998.0 35 1886564.0 36 3198611.0 37 6023993.0 38 7842506.0 39 2402335.0 40 4588.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.783048505528484 33.892332157431525 27.97623138040403 21.348387956635957 2 20.70218203706059 34.156285825509016 27.05999166492447 18.081540472505928 3 25.918516944461313 25.927222208333482 27.110296757809465 21.043964089395743 4 19.57763021220377 27.73503594329621 25.345858244925395 27.341475599574622 5 21.041510739280664 33.13262324146278 26.339663019289496 19.486202999967066 6 27.85285404909123 27.100496038641342 25.959474610024785 19.08717530224264 7 20.463492973564293 26.178424874019207 33.046768180231034 20.311313972185463 8 26.96723166977057 26.692615793402215 26.069583320398493 20.270569216428726 9 26.475557679464423 25.567346256454943 27.38855270506717 20.56854335901346 10-11 20.082429061886426 30.10764273404763 29.653801442786737 20.156126761279207 12-13 20.019431797296676 26.73722211492789 29.582162443863925 23.661183643911507 14-15 20.002383559626697 33.50685940452836 26.227186370805367 20.26357066503958 16-17 23.54046893453614 26.522166039979243 26.290406847289272 23.646958178195344 18-19 20.07483407488672 29.990894149905543 26.307256447184134 23.6270153280236 20-21 23.49261766877487 30.075870990484344 26.260212482871122 20.171298857869665 22-23 23.50238383434903 26.67528768235725 29.659146509144545 20.16318197414918 24-25 20.194385281799523 29.9200899503811 26.12913550694076 23.756389260878617 26-27 20.186890869544538 26.545653479282244 33.05465374423076 20.212801906942456 28-29 20.185080777980676 26.661515508487682 33.002433147007494 20.15097056652415 30-31 20.17241436149448 26.585838091295177 33.00514774704337 20.236599800166978 32-33 20.26270048656588 26.681012667052002 32.8312216028321 20.225065243550024 34-35 20.280072892076156 26.78816250320395 32.750232895856335 20.18153170886356 36-37 20.25143687079034 26.73926582138381 32.78628733075194 20.223009977073904 38-39 20.269727879289206 26.73812869862109 32.77515021130269 20.216993210787027 40-41 20.27773815466969 26.80471065609531 32.747833440216986 20.169717749018012 42-43 20.310137667786215 26.815923986356122 32.683959889283955 20.189978456573705 44-45 20.32461771675548 26.883444372114685 32.63592063691295 20.156017274216882 46-47 20.36312291393008 26.982756440271498 32.538841911287136 20.115278734511286 48-49 20.398458260653783 26.987359957153608 32.444215520704475 20.16996626148813 50-51 20.428766353876977 27.066952441993063 32.33407835740221 20.17020284672774 52-53 20.558692415255596 27.20146201116783 32.02877298253252 20.21107259104405 54-55 20.909618907006912 27.46101224115388 31.20528590817112 20.424082943668083 56-57 21.335829849321854 28.077090943031912 29.93459300042031 20.652486207225927 58-59 21.467352176933048 28.629935615711144 29.167141564426718 20.735570642929094 60-61 21.485655595030416 28.947693615585994 28.76982580644175 20.796824982941835 62-63 21.4964867972253 29.129326565779962 28.537342785160973 20.836843851833763 64-65 21.47491417582331 29.222089504531397 28.461525332971433 20.841470986673862 66-67 21.43639061323922 29.07791943170497 28.59619069240413 20.889499262651682 68-69 21.376406892910094 28.970496326538935 28.76830302422685 20.88479375632412 70-71 21.317899635670134 28.88419821219147 28.882488057721766 20.915414094416633 72-73 21.287641698900885 28.79024309568295 28.899046574042597 21.02306863137357 74-75 21.348683153753754 28.847398933911663 28.74159985778758 21.062318054547006 76-77 21.41510736172749 28.952113872966844 28.564888051323145 21.067890713982518 78-79 21.50728741385513 29.10375064064762 28.209617084009697 21.179344861487557 80-81 21.574610597661387 29.298757613873832 27.857287019042897 21.269344769421885 82-83 21.623717654260336 29.45851654643292 27.50240155826243 21.415364241044312 84-85 21.650338303357795 29.536456146767254 27.25027923656166 21.562926313313298 86-87 21.704920594310675 29.560743764047743 27.061367917319867 21.672967724321715 88-89 21.819715387767477 29.580463521711664 26.861483140899868 21.73833794962099 90-91 21.872323447285194 29.52498731738999 26.744928638768904 21.85776059655591 92-93 21.95135931030858 29.506966592934415 26.65606647122374 21.885607625533268 94-95 22.006541735674002 29.492267189166675 26.574078961344412 21.92711211381491 96-97 22.058559599036247 29.42313724657901 26.52448901978327 21.993814134601468 98-99 22.110841046938823 29.361554364878344 26.510599045232418 22.017005542950418 100 22.099758241933106 29.36161383661693 26.455116389230938 22.08351153221903 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20219.0 1 15144.5 2 8254.0 3 5974.5 4 5510.0 5 5513.0 6 5697.0 7 5833.5 8 6037.0 9 6463.0 10 7178.0 11 10225.0 12 109961.5 13 136523.0 14 64703.5 15 68716.5 16 79535.5 17 90927.5 18 102026.5 19 113366.5 20 127488.0 21 147444.5 22 174142.0 23 203419.5 24 233130.5 25 267627.5 26 308573.0 27 355189.0 28 412748.5 29 471283.5 30 517612.0 31 561749.5 32 611259.5 33 656780.5 34 693581.0 35 738446.5 36 794906.5 37 837752.0 38 867912.5 39 891514.0 40 905519.5 41 921145.5 42 948354.0 43 964884.0 44 972101.5 45 982589.5 46 983607.0 47 981332.0 48 966142.5 49 941667.5 50 927878.0 51 915637.0 52 889263.0 53 846945.0 54 789612.0 55 727440.5 56 664679.0 57 596731.0 58 524679.5 59 446825.5 60 365891.5 61 294213.5 62 229910.0 63 170031.0 64 122202.0 65 86471.0 66 60613.0 67 43378.5 68 31468.5 69 22474.0 70 16422.5 71 12574.0 72 9791.5 73 7432.5 74 5822.5 75 4885.0 76 3611.0 77 2096.5 78 1204.0 79 783.5 80 511.0 81 368.0 82 264.0 83 194.0 84 131.5 85 86.5 86 55.0 87 38.0 88 26.5 89 20.5 90 17.0 91 10.0 92 7.0 93 4.5 94 4.0 95 3.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10599130943377255 2 0.0 3 0.0 4 0.0 5 0.009817889313823285 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.38316265292762E-5 20-21 0.010949401915748705 22-23 0.0 24-25 8.842625037808661E-4 26-27 0.004969040166877333 28-29 0.0 30-31 0.0 32-33 1.7170145704482835E-6 34-35 0.0 36-37 1.7170145704482835E-6 38-39 0.0 40-41 0.00756344918282469 42-43 0.03925953815330001 44-45 0.015224768196164931 46-47 0.004847132132375505 48-49 0.0016277298127849729 50-51 0.0 52-53 0.001459462384881041 54-55 6.181252453613821E-5 56-57 9.048666786262455E-4 58-59 3.0906262268069104E-5 60-61 3.1077963725113935E-4 62-63 3.039115789693462E-4 64-65 7.211461195882791E-5 66-67 2.9360949154665653E-4 68-69 5.44293618832106E-4 70-71 4.653109485914849E-4 72-73 0.0013014970443997991 74-75 0.0016912593518915594 76-77 0.0025428985788339084 78-79 0.0046015990488014 80-81 0.004344046863234158 82-83 0.0076870742318969655 84-85 0.00253087947684077 86-87 0.002960133119452841 88-89 0.005755432840142647 90-91 0.005837849539524164 92-93 0.00572280956330413 94-95 0.002826205982957875 96-97 0.005734828665297268 98-99 0.008020175058563932 100 0.29787112171050917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9120312E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.473260358104156 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32059819444736 30.82317518888361 2 12.757383714585838 10.581805925665126 3 4.34483241086778 5.405830973647465 4 2.285593180525475 3.7916400419456138 5 1.3293138671228582 2.7565490054412276 6 0.9108013070065081 2.266433984398948 7 0.6484861131331234 1.8826383386009509 8 0.486952104066041 1.6156393115086038 9 0.38268507527249 1.4284077985742998 >10 2.209614060817518 17.03403697214633 >50 0.19508694035864912 5.710403193398542 >100 0.12557439564457062 9.361460772846886 >500 0.002469781806035476 0.6018330182672532 >1k 5.513833440206361E-4 0.47789224094973376 >5k 1.642028624040683E-5 0.03738705406892132 >10k+ 4.105071554872408E-5 6.224866179656386 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1727217 5.931313510651946 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 35579 0.12217932280395898 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 32429 0.11136213101013478 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005374255605503128 6.868058281793135E-6 0.0 0.0 3.4340291408965673E-6 2 0.005590599441379612 6.868058281793135E-6 0.0 0.0 6.868058281793135E-6 3 0.005927134297187475 1.0302087422689702E-5 0.0 3.4340291408965673E-6 6.868058281793135E-6 4 0.00597177667601913 1.7170145704482835E-5 0.0 1.0302087422689702E-5 6.868058281793135E-6 5 0.0059752107051600275 2.747223312717254E-5 0.0 1.0302087422689702E-5 1.0302087422689702E-5 6 0.0059752107051600275 3.0906262268069104E-5 0.0 1.373611656358627E-5 1.0302087422689702E-5 7 0.005978644734300924 3.0906262268069104E-5 0.0 1.373611656358627E-5 1.373611656358627E-5 8 0.0060061169674280965 3.434029140896567E-5 0.0 1.373611656358627E-5 1.373611656358627E-5 9 0.006009550996568993 4.120834969075881E-5 0.0 3.0906262268069104E-5 1.373611656358627E-5 10-11 0.006021570098562131 4.2925364261207094E-5 0.0 3.6057305979413956E-5 1.5453131134034552E-5 12-13 0.006030155171414372 4.9793422543000225E-5 0.0 4.120834969075881E-5 1.888716027493112E-5 14-15 0.006037023229696166 5.494446625434508E-5 0.0 4.120834969075881E-5 2.0604174845379404E-5 16-17 0.00604217427340751 6.009550996568993E-5 0.0 4.120834969075881E-5 2.747223312717254E-5 18-19 0.006066212477393786 7.554864109972448E-5 0.0 4.9793422543000225E-5 3.262327683851739E-5 20-21 0.0060868166522391656 8.928475766331075E-5 0.0 5.151043711344851E-5 3.434029140896567E-5 22-23 0.006128025001929925 9.958684508600045E-5 0.0 5.151043711344851E-5 3.949133512031052E-5 24-25 0.006277405269558925 1.0817191793824186E-4 0.0 5.151043711344851E-5 4.635939340210365E-5 26-27 0.007051778840831101 1.1847400536093157E-4 0.0 5.494446625434508E-5 4.807640797255194E-5 28-29 0.009967269581452286 1.253420636427247E-4 0.0 5.837849539524164E-5 4.9793422543000225E-5 30-31 0.017668079929912837 1.3049310735406956E-4 0.0 6.352953910658649E-5 5.666148082479336E-5 32-33 0.03347148203631884 1.4079519477675927E-4 0.0 6.524655367703478E-5 6.868058281793134E-5 34-35 0.0633234973581327 1.4766325305855238E-4 0.0 6.524655367703478E-5 7.383162652927619E-5 36-37 0.11252626688889872 1.6311638419258697E-4 0.0 6.524655367703478E-5 8.41337139519659E-5 38-39 0.18297365769982132 1.665504133334835E-4 0.0 7.039759738837963E-5 9.615281594510388E-5 40-41 0.2805859360298063 1.802865298970698E-4 0.0 8.585072852241419E-5 9.958684508600045E-5 42-43 0.40715394807583105 1.9573966103110432E-4 0.0 8.756774309286247E-5 9.958684508600045E-5 44-45 0.5674200880814739 2.094757775946906E-4 0.0 9.271878680420732E-5 1.0302087422689702E-4 46-47 0.7659876721101064 2.1806085044693202E-4 0.0 9.44358013746556E-5 1.0645490336779358E-4 48-49 1.0059833836945153 2.214948795878286E-4 0.0 1.0988893250869015E-4 1.0988893250869015E-4 50-51 1.287171991838549 2.2664592329917343E-4 0.0 1.1160594707913845E-4 1.0988893250869015E-4 52-53 1.6057073839044032 2.3007995244007E-4 0.0 1.2362504907227642E-4 1.15039976220035E-4 54-55 1.9652038755628718 2.4038203986275972E-4 0.0 1.27059078213173E-4 1.2019101993137986E-4 56-57 2.371379125333547 2.5755218556724254E-4 0.0 1.2877609278362127E-4 1.2362504907227644E-4 58-59 2.8235772336505187 2.609862147081391E-4 0.0 1.3392713649496612E-4 1.3392713649496612E-4 60-61 3.317838764914332 2.66137258419484E-4 0.0 1.459462384881041E-4 1.3736116563586268E-4 62-63 3.8476562339029883 2.8159038955351853E-4 0.0 1.5109728219944897E-4 1.4079519477675927E-4 64-65 4.411832881460885 2.936094915466565E-4 0.0 1.5281429676989726E-4 1.5109728219944897E-4 66-67 5.014489542557099 3.0219456439889794E-4 0.0 1.6139936962213867E-4 1.562483259107938E-4 68-69 5.657478875913142 3.0562859353979447E-4 0.0 1.665504133334835E-4 1.6483339876303523E-4 70-71 6.333567099143718 3.124966518215876E-4 0.0 1.7513548618572494E-4 1.665504133334835E-4 72-73 7.038456524778993 3.2279873924427735E-4 0.0 1.768525007561732E-4 1.768525007561732E-4 74-75 7.772052717017592 3.313838120965187E-4 0.0 1.802865298970698E-4 1.785695153266215E-4 76-77 8.532652397405633 3.4511992866010505E-4 0.0 1.9230563189020776E-4 1.785695153266215E-4 78-79 9.3186003638972 3.5027097237144987E-4 0.0 1.9402264646065605E-4 1.888716027493112E-4 80-81 10.127628440244735 3.5370500151234646E-4 0.0 1.991736901720009E-4 1.888716027493112E-4 82-83 10.95624250179737 3.6400708893503616E-4 0.0 2.008907047424492E-4 1.888716027493112E-4 84-85 11.800441904605968 3.760261909281741E-4 0.0 2.094757775946906E-4 1.9230563189020778E-4 86-87 12.662379784941866 3.81177234639519E-4 0.0 2.1290980673558716E-4 1.991736901720009E-4 88 13.318603866606924 3.81177234639519E-4 0.0 2.1634383587648375E-4 1.991736901720009E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 217070 0.0 86.15791 8 GTGGTAT 219800 0.0 85.47264 6 AGTGGTA 221495 0.0 84.827675 5 TGGTATC 220760 0.0 84.79016 7 GTATCAA 221395 0.0 84.33896 9 AAGCAGT 221425 0.0 83.14309 1 GCAGTGG 226420 0.0 83.0282 3 AGCAGTG 232975 0.0 80.5509 2 CAGTGGT 233005 0.0 80.48808 4 CAACGCA 214220 0.0 44.62831 12-13 ATCAACG 215375 0.0 44.44131 10-11 ACGCAGA 216825 0.0 44.21267 14-15 TCAACGC 213615 0.0 43.663563 12-13 AGAGTAC 219095 0.0 43.569584 18-19 AACGCAG 215480 0.0 43.373997 14-15 GAGTACT 213935 0.0 43.23215 20-21 CGCAGAG 217865 0.0 42.959583 16-17 TATCAAC 219855 0.0 42.437073 10-11 AGTACTT 226075 0.0 41.936646 20-21 GTACTTT 223740 0.0 41.342915 22-23 >>END_MODULE