FastQCFastQC Report
Thu 26 May 2016
SRR1660749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31912010
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15707614.922162533792136No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319950.10026005883051554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2110300.083.041835
GGTATCA2108200.083.0238958
GTGGTAT2115650.082.773726
TGGTATC2123050.082.14677
GTATCAA2139300.081.700579
GCAGTGG2190400.080.558243
AAGCAGT2198100.080.104651
CAGTGGT2252600.077.70854
AGCAGTG2297100.076.862762
CAACGCA2086850.043.06544512-13
GAGTACT1978600.043.0506220-21
ATCAACG2104100.042.8742310-11
ACGCAGA2107650.042.63854614-15
AGAGTAC2087000.042.40284318-19
TCAACGC2088800.041.95933512-13
AACGCAG2097500.041.844614-15
AGTACTT2101850.041.5766520-21
CGCAGAG2121500.041.24093616-17
TATCAAC2151100.040.55741510-11
GTACTTT2118250.040.2332122-23
Produced by FastQC (version 0.11.5)