FastQCFastQC Report
Thu 26 May 2016
SRR1660749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31912010
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17258905.40827732255035No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT369620.1158247318172688No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT321590.10077397193094387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2292400.083.260468
AGTGGTA2303800.083.218195
GTGGTAT2309200.082.982366
TGGTATC2321300.082.246157
GTATCAA2326050.081.9327249
GCAGTGG2364150.081.213143
AAGCAGT2330000.080.710821
CAGTGGT2430400.078.7499164
AGCAGTG2479150.077.377682
CAACGCA2268800.043.11029412-13
GAGTACT2183450.042.95997620-21
ATCAACG2281650.042.92722710-11
ACGCAGA2293300.042.7096414-15
AGAGTAC2289500.042.45728318-19
TCAACGC2267600.041.9829512-13
AACGCAG2274300.041.9245814-15
AGTACTT2303050.041.84538320-21
CGCAGAG2306400.041.3899916-17
TATCAAC2336200.040.76625410-11
GTACTTT2318150.040.44302722-23
Produced by FastQC (version 0.11.5)