##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660749_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31912010 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.020617002814927 33.0 31.0 34.0 27.0 34.0 2 31.802999341000458 34.0 31.0 34.0 28.0 34.0 3 32.30344597535536 34.0 31.0 34.0 30.0 34.0 4 35.83308096230855 37.0 35.0 37.0 35.0 37.0 5 35.88818889816091 37.0 35.0 37.0 35.0 37.0 6 35.913087549170356 37.0 35.0 37.0 35.0 37.0 7 35.899689646625205 37.0 35.0 37.0 35.0 37.0 8 35.90902071038459 37.0 35.0 37.0 35.0 37.0 9 37.61593143145794 39.0 37.0 39.0 35.0 39.0 10-11 37.55750444738517 39.0 37.0 39.0 35.0 39.0 12-13 37.6258138707026 39.0 37.0 39.0 35.0 39.0 14-15 38.959628240276935 40.0 38.0 41.0 35.0 41.0 16-17 38.9784520154011 40.0 38.0 41.0 35.0 41.0 18-19 38.97218061475915 40.0 38.5 41.0 35.0 41.0 20-21 38.90549600291551 40.0 38.0 41.0 35.0 41.0 22-23 38.8003336674813 40.0 38.0 41.0 34.5 41.0 24-25 38.757443373200246 40.0 38.0 41.0 34.0 41.0 26-27 38.72527336886645 40.0 38.0 41.0 34.0 41.0 28-29 38.62809235457121 40.0 38.0 41.0 34.0 41.0 30-31 38.53089468510445 40.0 38.0 41.0 34.0 41.0 32-33 38.42691555624356 40.0 38.0 41.0 34.0 41.0 34-35 38.31814508080187 40.0 38.0 41.0 34.0 41.0 36-37 38.13088686673136 40.0 38.0 41.0 33.0 41.0 38-39 37.70944450067545 40.0 36.0 41.0 33.0 41.0 40-41 37.56129120353121 40.0 36.0 41.0 33.0 41.0 42-43 37.3978506994702 40.0 35.5 41.0 32.5 41.0 44-45 37.2726305707475 39.5 35.0 41.0 32.0 41.0 46-47 37.134764529091086 39.0 35.0 41.0 31.0 41.0 48-49 37.24329843215767 39.5 35.0 41.0 32.0 41.0 50-51 37.13937440794234 39.0 35.0 41.0 31.5 41.0 52-53 36.86877767962595 39.0 35.0 41.0 31.0 41.0 54-55 36.36179386694853 39.0 35.0 41.0 29.0 41.0 56-57 36.03779276203536 39.0 35.0 41.0 27.5 41.0 58-59 35.83269143811374 39.0 35.0 41.0 27.0 41.0 60-61 35.63263957676122 39.0 35.0 41.0 27.0 41.0 62-63 35.345771309923755 38.0 35.0 40.5 26.0 41.0 64-65 35.10209306778232 37.5 34.5 40.0 26.0 41.0 66-67 34.83995539610322 37.0 34.0 40.0 26.0 41.0 68-69 34.556412162693604 37.0 34.0 39.5 26.0 41.0 70-71 34.237603146903 36.0 34.0 39.0 25.5 41.0 72-73 33.92240784895718 36.0 34.0 39.0 25.0 41.0 74-75 33.566794147407194 35.0 33.5 38.5 25.0 40.0 76-77 31.4910437637742 33.5 30.5 35.5 21.0 38.5 78-79 32.75484812771116 35.0 33.0 37.0 24.0 39.0 80-81 32.741031652346564 35.0 33.0 36.0 24.0 39.0 82-83 32.52612920652757 35.0 33.0 36.0 24.0 37.5 84-85 32.30206282838342 35.0 33.0 36.0 24.0 37.0 86-87 32.10672392619581 35.0 33.0 35.0 23.5 37.0 88-89 31.899309726964866 35.0 33.0 35.0 23.0 36.0 90-91 31.75113899124499 35.0 33.0 35.0 22.5 36.0 92-93 31.637468683420444 35.0 33.0 35.0 20.5 36.0 94-95 31.553080501666926 35.0 33.0 35.0 20.0 35.5 96-97 31.461586954253274 35.0 33.0 35.0 20.0 35.0 98-99 31.35673273165808 35.0 32.0 35.0 20.0 35.0 100 31.177277457609218 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7531787469245401 1101 2 1.193779877247767 1101 3 0.767093081129353 1101 4 0.6217405969994871 1101 5 0.4779529758707852 1101 6 0.423524019458398 1101 7 0.394175586153942 1101 8 0.3951488600982245 1101 9 0.4836224168128993 1101 10-11 0.4902534722920535 1101 12-13 0.44981207052357064 1101 14-15 0.6189018275284681 1101 16-17 0.6172931427810155 1101 18-19 0.5391759711657471 1101 20-21 0.5886686181403675 1101 22-23 0.5587296952756233 1101 24-25 0.5904248496442293 1101 26-27 0.5745990298251797 1101 28-29 0.5770168838497582 1101 30-31 0.5546366859546339 1101 32-33 0.5442255166953913 1101 34-35 0.5521396274699484 1101 36-37 0.5512404575924208 1101 38-39 0.6285010223834675 1101 40-41 0.6146660921078038 1101 42-43 0.5692612987395904 1101 44-45 0.5440505754305534 1101 46-47 0.4927513620608508 1101 48-49 0.4605840695072274 1101 50-51 0.47555726157005296 1101 52-53 0.48966461291431784 1101 54-55 0.5214331578409386 1101 56-57 0.5568425013286245 1101 58-59 0.5460105361668965 1101 60-61 0.5035298880610242 1101 62-63 0.5761337619232876 1101 64-65 0.5416253681325855 1101 66-67 0.5033226151683152 1101 68-69 0.52008505864044 1101 70-71 0.5302325857341188 1101 72-73 0.5064680839387918 1101 74-75 0.4458690094306448 1101 76-77 0.34678321696524605 1101 78-79 0.48734682478636415 1101 80-81 0.5052947829408438 1101 82-83 0.4587955200182918 1101 84-85 0.44642687610158305 1101 86-87 0.39861175471603616 1101 88-89 0.4538301950683028 1101 90-91 0.4745874157841037 1101 92-93 0.4162941375057798 1101 94-95 0.33245844991256845 1101 96-97 0.3500902949614826 1101 98-99 0.3711131061201769 1101 100 0.3192394428974623 1102 1 1.6703613233257855 1102 2 1.1522483685802634 1102 3 0.7130292299106102 1102 4 0.5675943347375352 1102 5 0.42288868485324116 1102 6 0.40982676181669575 1102 7 0.35595243634334395 1102 8 0.3899892478147535 1102 9 0.4781175861196729 1102 10-11 0.4588274522820015 1102 12-13 0.38907823707017286 1102 14-15 0.5574736636963209 1102 16-17 0.5428299258813993 1102 18-19 0.5244702501558152 1102 20-21 0.5263399544508829 1102 22-23 0.5331269108290542 1102 24-25 0.5606633183247212 1102 26-27 0.5464205850104662 1102 28-29 0.5545743138484411 1102 30-31 0.5581959823396261 1102 32-33 0.5347680140274349 1102 34-35 0.5090310066253849 1102 36-37 0.5969098739210992 1102 38-39 0.6465639372563885 1102 40-41 0.6008598148075706 1102 42-43 0.5902829087815107 1102 44-45 0.5844956707281668 1102 46-47 0.6037042279253768 1102 48-49 0.5531134170129093 1102 50-51 0.5426351847739284 1102 52-53 0.49473229306236277 1102 54-55 0.5374285454707746 1102 56-57 0.5800543566647676 1102 58-59 0.5549655805329863 1102 60-61 0.4969028137949323 1102 62-63 0.5470069438124057 1102 64-65 0.5230259933089485 1102 66-67 0.47784863344439543 1102 68-69 0.47389926492972023 1102 70-71 0.46221776498759226 1102 72-73 0.41486225085110107 1102 74-75 0.41825584005766103 1102 76-77 0.36228718067159704 1102 78-79 0.5011501385678301 1102 80-81 0.48801954928787694 1102 82-83 0.4475144917403284 1102 84-85 0.43722062622966007 1102 86-87 0.451485390591003 1102 88-89 0.487341255456311 1102 90-91 0.4949859144781108 1102 92-93 0.4465290121315917 1102 94-95 0.3920365110461752 1102 96-97 0.4170387218532099 1102 98-99 0.42142405490332635 1102 100 0.3413673754805622 1103 1 1.673453096466048 1103 2 1.122037077738181 1103 3 0.6867662793126144 1103 4 0.553901608569106 1103 5 0.42989179865691796 1103 6 0.37856844123064093 1103 7 0.36059955984341485 1103 8 0.3823325986802857 1103 9 0.45856521789031035 1103 10-11 0.4193986066462756 1103 12-13 0.3949732914833888 1103 14-15 0.5653313498438379 1103 16-17 0.5176616480945029 1103 18-19 0.44676749394325554 1103 20-21 0.44936917625496875 1103 22-23 0.4795055646568045 1103 24-25 0.5297663043040828 1103 26-27 0.5052136491560688 1103 28-29 0.5253315028193057 1103 30-31 0.5225677932111097 1103 32-33 0.5007819592349279 1103 34-35 0.5293416207862478 1103 36-37 0.5308488838361498 1103 38-39 0.5873713944176089 1103 40-41 0.5459117506601885 1103 42-43 0.5293931588331375 1103 44-45 0.5215004563647554 1103 46-47 0.5646049681195606 1103 48-49 0.519575969922009 1103 50-51 0.46335470328605766 1103 52-53 0.4557504321514685 1103 54-55 0.5431286543961065 1103 56-57 0.6119437101635228 1103 58-59 0.5688349919131497 1103 60-61 0.5825275281802078 1103 62-63 0.5707124714782594 1103 64-65 0.5330646492315481 1103 66-67 0.5274152938789314 1103 68-69 0.5462553088559119 1103 70-71 0.5085812436250094 1103 72-73 0.47952655145117973 1103 74-75 0.4510275412054412 1103 76-77 0.35548805193128885 1103 78-79 0.4358976363892637 1103 80-81 0.4945553244267131 1103 82-83 0.47486082342554425 1103 84-85 0.4639722858783273 1103 86-87 0.4439761582721289 1103 88-89 0.48954858013400226 1103 90-91 0.46283198120819335 1103 92-93 0.45693734450865975 1103 94-95 0.4308191402375705 1103 96-97 0.4128641467496017 1103 98-99 0.4769406423336946 1103 100 0.4314809254658023 1104 1 1.5848055261002898 1104 2 1.0643570550813273 1104 3 0.6208701375614822 1104 4 0.5195182474128615 1104 5 0.3860511547190555 1104 6 0.33753931968595907 1104 7 0.29839670870035206 1104 8 0.32169401244269125 1104 9 0.4355813565563622 1104 10-11 0.38828636530639216 1104 12-13 0.3566065061176431 1104 14-15 0.5171351436241878 1104 16-17 0.4816469667307075 1104 18-19 0.4244207279134855 1104 20-21 0.39260875238533544 1104 22-23 0.41365232791515183 1104 24-25 0.4465482362231157 1104 26-27 0.4242825263948049 1104 28-29 0.45348427154366533 1104 30-31 0.4296427755650498 1104 32-33 0.44210594149619453 1104 34-35 0.4147908614790268 1104 36-37 0.4538750119306414 1104 38-39 0.5184948415917248 1104 40-41 0.5129608253437894 1104 42-43 0.4796264864884705 1104 44-45 0.4749304231837712 1104 46-47 0.5086649794718028 1104 48-49 0.4974380334853947 1104 50-51 0.45669161596062935 1104 52-53 0.44321495998953964 1104 54-55 0.5113067740013975 1104 56-57 0.5340835170365565 1104 58-59 0.5029483869707931 1104 60-61 0.4938882828006399 1104 62-63 0.5710612011216227 1104 64-65 0.5371395005877986 1104 66-67 0.5503289366398292 1104 68-69 0.5429984064151583 1104 70-71 0.5364326818652927 1104 72-73 0.5035796677455053 1104 74-75 0.495820357676898 1104 76-77 0.40348559197843414 1104 78-79 0.49431608755924117 1104 80-81 0.46887603397477307 1104 82-83 0.4450639500450819 1104 84-85 0.45598371528767245 1104 86-87 0.4979041745513655 1104 88-89 0.5051570788420712 1104 90-91 0.4589198251466051 1104 92-93 0.46841595967352134 1104 94-95 0.4806248258844654 1104 96-97 0.46713566158208053 1104 98-99 0.4688294370875745 1104 100 0.3760683515480956 1105 1 1.5841362367232108 1105 2 1.0445491244525975 1105 3 0.6110882956531825 1105 4 0.5062594619260139 1105 5 0.3638440001686547 1105 6 0.28075126077724377 1105 7 0.235564946802306 1105 8 0.26535259894021834 1105 9 0.3571063861941681 1105 10-11 0.3049788251881651 1105 12-13 0.2787517206208818 1105 14-15 0.3983065117854707 1105 16-17 0.3453933261179145 1105 18-19 0.3231353302287232 1105 20-21 0.3264074849214893 1105 22-23 0.3097048792000976 1105 24-25 0.365134716263114 1105 26-27 0.3280720938589141 1105 28-29 0.3657268521721093 1105 30-31 0.37389168947366613 1105 32-33 0.3781203367835033 1105 34-35 0.37969975395261457 1105 36-37 0.4054882086849929 1105 38-39 0.45093156453563665 1105 40-41 0.42574108218316553 1105 42-43 0.3972438934858502 1105 44-45 0.41255733337015243 1105 46-47 0.444647711689484 1105 48-49 0.4231494271665923 1105 50-51 0.3988903893123279 1105 52-53 0.3436201189254291 1105 54-55 0.4446252909488493 1105 56-57 0.41045758557797996 1105 58-59 0.4348218974422977 1105 60-61 0.38071235990846475 1105 62-63 0.41547625037375013 1105 64-65 0.3837710152024414 1105 66-67 0.3874219603774094 1105 68-69 0.43314382772354776 1105 70-71 0.3937534831633158 1105 72-73 0.3178957286814281 1105 74-75 0.260454826296872 1105 76-77 0.25173470625881933 1105 78-79 0.351253862111399 1105 80-81 0.3697642832220964 1105 82-83 0.36549389613853833 1105 84-85 0.3867063071147925 1105 86-87 0.35167462612423606 1105 88-89 0.37130416275309486 1105 90-91 0.3813850681715998 1105 92-93 0.3572598134371958 1105 94-95 0.3329123978235806 1105 96-97 0.30892514062361087 1105 98-99 0.34248474557879405 1105 100 0.2829641831511971 1106 1 1.4267357009910668 1106 2 0.9011049544950573 1106 3 0.4940275839761057 1106 4 0.4417894687194561 1106 5 0.25682253218762696 1106 6 0.2562958070607877 1106 7 0.18808099699528213 1106 8 0.19421431687789692 1106 9 0.2681243931759738 1106 10-11 0.23677040350866463 1106 12-13 0.21648969185040556 1106 14-15 0.33165464283309376 1106 16-17 0.2668000523244629 1106 18-19 0.19811634262342182 1106 20-21 0.22013213943932186 1106 22-23 0.19428108315008785 1106 24-25 0.24488893557145275 1106 26-27 0.24291218029175354 1106 28-29 0.2721841461543022 1106 30-31 0.2701552404843781 1106 32-33 0.24191690590359372 1106 34-35 0.25357515259421604 1106 36-37 0.25046854547749575 1106 38-39 0.3504184608300349 1106 40-41 0.34681243067042544 1106 42-43 0.2796626651389218 1106 44-45 0.2543210833055056 1106 46-47 0.3159791631731821 1106 48-49 0.3285508190783162 1106 50-51 0.30010030300115886 1106 52-53 0.25819323572736863 1106 54-55 0.27374247817915887 1106 56-57 0.2696663161357975 1106 58-59 0.25426454208168536 1106 60-61 0.22957890978688056 1106 62-63 0.28553314427324494 1106 64-65 0.295424426334165 1106 66-67 0.2388348423163933 1106 68-69 0.24637442130308074 1106 70-71 0.27727720777973985 1106 72-73 0.2539991129184287 1106 74-75 0.19057617874357646 1106 76-77 0.15937728917256422 1106 78-79 0.2854467546359132 1106 80-81 0.265778513074018 1106 82-83 0.240466638081287 1106 84-85 0.2714391788270234 1106 86-87 0.23159960330944074 1106 88-89 0.2798545856311101 1106 90-91 0.27844370216032743 1106 92-93 0.25023497369722136 1106 94-95 0.2754983957353865 1106 96-97 0.26271583448620106 1106 98-99 0.2906668654224447 1106 100 0.2505767595744892 1107 1 1.5925184164552704 1107 2 1.0523251424225073 1107 3 0.6109200084577608 1107 4 0.517097496369658 1107 5 0.364968784416412 1107 6 0.3362402368739268 1107 7 0.2767816862363688 1107 8 0.2985263447020756 1107 9 0.3594266683012606 1107 10-11 0.31378874845116655 1107 12-13 0.32156060069265635 1107 14-15 0.4476709280190221 1107 16-17 0.41694783657595735 1107 18-19 0.36678733902603256 1107 20-21 0.3654160429230586 1107 22-23 0.3741426501146208 1107 24-25 0.42693049377466963 1107 26-27 0.39166411674243307 1107 28-29 0.34885356116394206 1107 30-31 0.3767381159786112 1107 32-33 0.4080210936151474 1107 34-35 0.4384490195479813 1107 36-37 0.5056870137165888 1107 38-39 0.4934716590643049 1107 40-41 0.49510131465530804 1107 42-43 0.4601902359312007 1107 44-45 0.453493641660927 1107 46-47 0.49917936854173206 1107 48-49 0.47774366225594633 1107 50-51 0.442547035985811 1107 52-53 0.39485530473562847 1107 54-55 0.4781676944118516 1107 56-57 0.3896929866345076 1107 58-59 0.3407712565690062 1107 60-61 0.321401931659139 1107 62-63 0.3864514081321744 1107 64-65 0.3896419835306588 1107 66-67 0.39219136693009204 1107 68-69 0.36524020146693204 1107 70-71 0.4266773156180719 1107 72-73 0.41034633536373377 1107 74-75 0.3700924628198905 1107 76-77 0.301640183440405 1107 78-79 0.4179608043120382 1107 80-81 0.39871840421562865 1107 82-83 0.35557177605792134 1107 84-85 0.3398339144087572 1107 86-87 0.347290145476137 1107 88-89 0.3962180063120364 1107 90-91 0.3578397188172069 1107 92-93 0.39718143485703905 1107 94-95 0.36956737875623347 1107 96-97 0.3176079677517656 1107 98-99 0.37837020307002334 1107 100 0.279819774374392 1108 1 1.4054060765045762 1108 2 0.8995646915859616 1108 3 0.4676147459168547 1108 4 0.38662936979137896 1108 5 0.22924742772352857 1108 6 0.16445068905292004 1108 7 0.14681626104611922 1108 8 0.15634619504226066 1108 9 0.17375419868348985 1108 10-11 0.14305373861287052 1108 12-13 0.11813409769887784 1108 14-15 0.17730253934816886 1108 16-17 0.157289259679537 1108 18-19 0.1281956767639727 1108 20-21 0.15961162069910984 1108 22-23 0.11294706055583958 1108 24-25 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2316 48-49 -1.7509401911981755 2316 50-51 -1.561960111424895 2316 52-53 -1.3614775140775919 2316 54-55 -1.2312387546096915 2316 56-57 -1.407524881505303 2316 58-59 -1.2402498060458669 2316 60-61 -1.1604813624286834 2316 62-63 -2.2128914314406316 2316 64-65 -1.5923327127567717 2316 66-67 -1.2862463442617482 2316 68-69 -1.203677216584289 2316 70-71 -1.0830693206430269 2316 72-73 -1.0568346021926942 2316 74-75 -1.1160230506076374 2316 76-77 -0.9796488236974596 2316 78-79 -1.260212086989398 2316 80-81 -1.1321151312395799 2316 82-83 -1.0393441718787173 2316 84-85 -0.9668533874495182 2316 86-87 -1.1129141443532546 2316 88-89 -1.0786847427014017 2316 90-91 -1.0340106937824878 2316 92-93 -0.9767858116215251 2316 94-95 -0.9620966230149897 2316 96-97 -0.9670822526158318 2316 98-99 -0.9888375611400093 2316 100 -0.945007176095249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 52.0 7 873.0 8 1094.0 9 488.0 10 228.0 11 252.0 12 338.0 13 912.0 14 3355.0 15 9275.0 16 20403.0 17 32946.0 18 44527.0 19 60708.0 20 88555.0 21 131954.0 22 195283.0 23 304948.0 24 480017.0 25 574283.0 26 553837.0 27 485180.0 28 460368.0 29 495215.0 30 576365.0 31 691740.0 32 844062.0 33 1062849.0 34 1419546.0 35 2082222.0 36 3530051.0 37 6707908.0 38 8589076.0 39 2458525.0 40 4575.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.707147505438304 33.30986577125417 27.900512719647647 22.082474003659886 2 21.10132204145085 33.55079795976499 27.055506688547666 18.29237331023649 3 25.660505245517285 26.03807155989234 26.710953023642197 21.59047017094818 4 19.658266589913953 27.909962424804956 25.396049951099915 27.035721034181176 5 21.47890547242165 32.566003476828755 26.12826371073231 19.826827340017285 6 27.812682435233633 26.991684321984106 25.817862929975266 19.377770312806998 7 20.86660790091254 26.081143118217874 32.35927476833957 20.692974212530014 8 26.82468449966016 26.643448657731057 25.942311374307042 20.589555468301747 9 26.271065344990806 25.432030135362833 27.36586006334292 20.93104445630344 10-11 20.522229718529168 29.71811709760683 29.24597353786239 20.513679646001613 12-13 20.46048807329905 26.592229696593854 29.11047596187141 23.836806268235687 14-15 20.418637058587034 32.89767394783343 26.010812230254377 20.672876763325156 16-17 23.700824548500705 26.426317239183618 26.04795028580149 23.82490792651419 18-19 20.537835465603994 29.608390138001422 26.087658588511715 23.76611580788287 20-21 23.682451675038486 29.667583772373384 26.071440978543187 20.578523574044944 22-23 23.698131205148158 26.57754870345052 29.161450500924257 20.562869590477064 24-25 20.584829989647847 29.59946829563566 25.885813701947765 23.92988801276873 26-27 20.620732052891423 26.405542319303223 32.33993829767615 20.633787330129213 28-29 20.62115799036162 26.547187093511187 32.258989640577326 20.572665275549866 30-31 20.655607402980884 26.420285027486518 32.29250053506501 20.63160703446759 32-33 20.683031247483314 26.561539056925593 32.09384961962596 20.66158007596513 34-35 20.689085394495677 26.613978561676312 32.01567685645624 20.681259187371776 36-37 20.657391997558285 26.549902372179 32.14138814822382 20.651317482038895 38-39 20.685350123668172 26.59383097460799 32.051362480771346 20.669456420952486 40-41 20.750169553209215 26.651034902522046 32.00184477401542 20.596950770253322 42-43 20.716526311534334 26.672999263609114 31.941948867942234 20.668525556914318 44-45 20.747480441083336 26.698382155896383 31.93388560814282 20.62025179487746 46-47 20.771651629487533 26.77310657551746 31.856461369941936 20.598780425053075 48-49 20.842386887410903 26.77861188666744 31.770700789035278 20.608300436886378 50-51 20.81635096003041 26.875306506860586 31.689448580644093 20.618893952464916 52-53 20.97451147677416 26.946042137052 31.374080335186388 20.70536605098745 54-55 21.290361397079906 27.169598323388794 30.62917260879062 20.910867670740686 56-57 21.59973511947192 27.715060274593878 29.55507557554318 21.13012903039102 58-59 21.716725888899106 28.23390867172796 28.887018746113142 21.162346693259796 60-61 21.801962768544104 28.521841850693157 28.425459350784465 21.25073602997827 62-63 21.866382923198238 28.724358644123114 28.13233072377585 21.27692770890279 64-65 21.780459396710008 28.844048958035607 28.15659872969975 21.218892915554637 66-67 21.73182486206835 28.799479116112465 28.20241050751255 21.26628551430664 68-69 21.600128579253926 28.742969046703692 28.372662863594922 21.284239510447463 70-71 21.51474884834761 28.67591769825922 28.454998296950023 21.354335156443145 72-73 21.477831144621444 28.574237669443004 28.5027762304485 21.445154955487055 74-75 21.53144698880796 28.571167357721666 28.41831637765019 21.479069275820184 76-77 21.571776937616665 28.63633098183452 28.326037176750518 21.4658549037983 78-79 21.59424583103629 28.805621698742563 28.001341123103796 21.59879134711736 80-81 21.650165222315653 28.991702172325528 27.638119209200468 21.720013396158354 82-83 21.607746257120418 29.1594682186483 27.284029636671736 21.94875588755955 84-85 21.56308004211624 29.222550704875417 27.061347555129263 22.153021697879083 86-87 21.536144738774286 29.253841504867495 26.885004641963967 22.325009114394252 88-89 21.562594488929676 29.25280350030281 26.702135732440148 22.48246627832737 90-91 21.486449808319545 29.211391087954837 26.63098083026213 22.67117827346349 92-93 21.466635600329802 29.222317872555525 26.553175300319236 22.757871226795437 94-95 21.452489973522564 29.26843006903625 26.436048610935387 22.8430313465058 96-97 21.42362865219584 29.1980322218879 26.36812704751319 23.01021207840307 98-99 21.37417678971477 29.15411372766866 26.35014070719822 23.12156877541835 100 21.311598658982305 29.126495094391576 26.29058495005757 23.27132129656855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 20606.0 1 15267.5 2 8150.0 3 6101.0 4 5677.0 5 5548.0 6 5735.5 7 5933.5 8 6007.0 9 6322.0 10 6878.5 11 9644.5 12 108292.0 13 134446.5 14 63400.0 15 67275.0 16 78007.5 17 89000.0 18 99129.0 19 109962.5 20 122453.0 21 140255.5 22 164952.0 23 194780.0 24 228166.0 25 264918.5 26 306215.5 27 357314.0 28 420459.0 29 484567.5 30 537252.5 31 585061.0 32 636595.0 33 679751.0 34 716177.5 35 764507.5 36 818412.0 37 863856.0 38 910137.5 39 942487.0 40 960488.0 41 994084.0 42 1038995.5 43 1058207.5 44 1062336.5 45 1081200.5 46 1096834.0 47 1105451.0 48 1100895.0 49 1088618.5 50 1081239.5 51 1070111.0 52 1049879.5 53 1001829.5 54 928492.0 55 854044.5 56 779074.5 57 696053.0 58 607159.0 59 514948.5 60 421828.0 61 338943.0 62 265602.5 63 197281.0 64 143021.5 65 103643.0 66 74490.0 67 54601.5 68 40212.5 69 29804.0 70 22558.0 71 17246.5 72 13850.0 73 10824.5 74 8294.5 75 6820.0 76 5120.5 77 3147.0 78 1804.0 79 1163.0 80 762.0 81 525.0 82 357.0 83 229.0 84 146.0 85 109.5 86 79.5 87 63.0 88 41.5 89 21.0 90 18.0 91 17.0 92 14.0 93 12.5 94 7.0 95 4.0 96 4.0 97 3.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10643328326858759 2 0.0 3 0.0 4 0.0 5 0.009548129371982524 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.267232932052854E-5 20-21 0.01088775041120882 22-23 0.0 24-25 9.134491998467035E-4 26-27 0.004833603398845763 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.007553582491356702 42-43 0.03920624241468964 44-45 0.015315550477704162 46-47 0.004850838289408909 48-49 0.0015950107812074512 50-51 0.0 52-53 0.0014148278344109319 54-55 6.267232932052854E-5 56-57 8.2884155526399E-4 58-59 2.5068931728211414E-5 60-61 3.023939889715502E-4 62-63 2.6949101607827273E-4 64-65 8.460764458271354E-5 66-67 3.1179483836962953E-4 68-69 5.468160733216116E-4 70-71 4.5594119580684515E-4 72-73 0.0012346448876144121 74-75 0.0016811852340231782 76-77 0.0025147272139862077 78-79 0.004518674944010108 80-81 0.004289920941990179 82-83 0.007376533161026209 84-85 0.002517860830452234 86-87 0.002970668409793053 88-89 0.005817558969178062 90-91 0.0058629964079354455 92-93 0.005640509638847569 94-95 0.00274974844893819 96-97 0.005660878145876741 98-99 0.007783903301609644 100 0.3004354786802837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.191201E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.56893729473335 #Duplication Level Percentage of deduplicated Percentage of total 1 65.7643541329177 24.04932542515306 2 16.65671632547026 12.182368296845667 3 6.353696872514518 6.970438275621806 4 3.0916762395330326 4.522372581564019 5 1.8518548546325646 3.38601820290029 6 1.151020333592147 2.5254954242456575 7 0.8245869884858784 2.1107988913195173 8 0.6301859686605576 1.8436184937574984 9 0.486669287703875 1.6017280798784 >10 2.8302034465634422 18.59208211409132 >50 0.19629583386026622 4.972570540909179 >100 0.1611312437097761 10.71137006057968 >500 8.481481635849197E-4 0.18959760941341178 >1k 6.922727659914084E-4 0.5352879735266778 >5k 1.701285673591126E-5 0.03415871150360222 >10k+ 5.103856980568147E-5 5.772769318690257 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1725890 5.40827732255035 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36962 0.1158247318172688 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 32159 0.10077397193094387 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09185256585216663 6.267232932052854E-6 0.0 0.0 3.133616466026427E-5 2 0.09721731724200387 1.5668082330132135E-5 0.0 3.133616466026427E-6 3.133616466026427E-5 3 0.10376970927246514 1.8801698796158564E-5 0.0 3.133616466026427E-6 3.133616466026427E-5 4 0.10485394056971027 3.760339759231713E-5 0.0 3.133616466026427E-6 3.4469781126290696E-5 5 0.10499808692714749 4.70042469903964E-5 0.0 3.133616466026427E-6 3.4469781126290696E-5 6 0.1051171643528565 5.327147992244926E-5 0.0 3.133616466026427E-6 4.073701405834355E-5 7 0.10525504347736166 5.953871285450212E-5 0.0 3.133616466026427E-6 4.073701405834355E-5 8 0.10566868085087715 5.953871285450212E-5 0.0 3.133616466026427E-6 4.073701405834355E-5 9 0.10580029274245026 7.207317871860782E-5 0.0 3.133616466026427E-6 4.387063052436998E-5 10-11 0.10588960081173202 8.774126104873996E-5 0.0 4.700424699039641E-6 4.70042469903964E-5 12-13 0.10597577526454774 9.087487751476639E-5 0.0 6.267232932052854E-6 5.79719046214889E-5 14-15 0.10608388503262564 9.400849398079282E-5 0.0 6.267232932052854E-6 5.953871285450212E-5 16-17 0.10618886118423754 1.0654295984489852E-4 0.0 6.267232932052854E-6 6.110552108751533E-5 18-19 0.1063988134874613 1.1907742570900423E-4 0.0 1.2534465864105709E-5 6.423913755354175E-5 20-21 0.1065272917625684 1.2534465864105708E-4 0.0 1.2534465864105709E-5 6.893956225258139E-5 22-23 0.10665420322944245 1.3004508334009672E-4 0.0 1.2534465864105709E-5 8.617445281572675E-5 24-25 0.1069910669995403 1.3787912450516278E-4 0.0 1.2534465864105709E-5 9.087487751476639E-5 26-27 0.10798912384396972 1.4727997390324207E-4 0.0 1.5668082330132135E-5 9.24416857477796E-5 28-29 0.1105602561543444 1.61381248000361E-4 0.0 1.5668082330132135E-5 1.0027572691284567E-4 30-31 0.11682122185346519 1.7234890563145347E-4 0.0 1.8801698796158564E-5 1.0027572691284567E-4 32-33 0.1300215812166015 1.8018294679651955E-4 0.0 1.8801698796158564E-5 1.0184253514585888E-4 34-35 0.15477088406527822 2.068186867577442E-4 0.0 1.8801698796158564E-5 1.0810976807791173E-4 36-37 0.19533084879329132 2.209199608548631E-4 0.0 2.5068931728211417E-5 1.159438092429778E-4 38-39 0.2557814440394071 2.3502123495198202E-4 0.0 2.5068931728211417E-5 1.2221104217503067E-4 40-41 0.33791353161395976 2.3502123495198202E-4 0.0 2.5068931728211417E-5 1.2221104217503067E-4 42-43 0.4445881033504314 2.538229337481406E-4 3.133616466026427E-6 2.8202548194237842E-5 1.2534465864105708E-4 44-45 0.5806842000864252 2.6009016668019345E-4 3.133616466026427E-6 2.8202548194237842E-5 1.2534465864105708E-4 46-47 0.7500765385821826 2.6949101607827273E-4 3.133616466026427E-6 2.9769356427251055E-5 1.2534465864105708E-4 48-49 0.9580593638570557 2.914263313404577E-4 3.133616466026427E-6 3.133616466026427E-5 1.3004508334009672E-4 50-51 1.202356103548476 3.023939889715502E-4 3.133616466026427E-6 3.133616466026427E-5 1.4101274097118923E-4 52-53 1.4760602669653213 3.274629206997616E-4 3.133616466026427E-6 3.133616466026427E-5 1.504135903692685E-4 54-55 1.7876059828259017 3.4156419479688054E-4 3.133616466026427E-6 3.133616466026427E-5 1.5511401506830813E-4 56-57 2.143233848322309 3.634995100590655E-4 3.133616466026427E-6 3.290297289327749E-5 1.629480562333742E-4 58-59 2.5462482620179676 3.760339759231713E-4 3.133616466026427E-6 3.760339759231713E-5 1.6764848093241385E-4 60-61 2.990034159553096 3.9796929118535623E-4 3.133616466026427E-6 3.760339759231713E-5 1.7078209739844027E-4 62-63 3.4663689313208415 4.0266971588439585E-4 3.133616466026427E-6 3.760339759231713E-5 1.723489056314535E-4 64-65 3.976064183985904 4.1050375704946196E-4 3.133616466026427E-6 3.760339759231713E-5 1.754825220974799E-4 66-67 4.525318210918083 4.261718393795941E-4 3.133616466026427E-6 3.760339759231713E-5 1.8174975502953278E-4 68-69 5.116034684120493 4.371394970106866E-4 3.133616466026427E-6 3.760339759231713E-5 1.8645017972857242E-4 70-71 5.745728645735571 4.434067299427394E-4 3.133616466026427E-6 3.760339759231713E-5 1.9428422089363848E-4 72-73 6.407705124183654 4.543743875738319E-4 3.133616466026427E-6 3.760339759231713E-5 1.9428422089363848E-4 74-75 7.099371051839104 4.6690885343793765E-4 3.133616466026427E-6 3.760339759231713E-5 1.9428422089363848E-4 76-77 7.824176540431017 4.888441687001227E-4 3.133616466026427E-6 3.760339759231713E-5 1.9428422089363848E-4 78-79 8.575895094041396 4.966782098651887E-4 3.133616466026427E-6 4.230382229135677E-5 1.974178373596649E-4 80-81 9.356801718224581 5.092126757292943E-4 3.133616466026427E-6 4.70042469903964E-5 2.0211826205870454E-4 82-83 10.156992618139691 5.264475662924398E-4 3.133616466026427E-6 4.70042469903964E-5 2.099523032237706E-4 84-85 10.977542624234575 5.28014374525453E-4 3.133616466026427E-6 4.70042469903964E-5 2.209199608548631E-4 86-87 11.81990103412477 5.35848415690519E-4 3.133616466026427E-6 4.70042469903964E-5 2.2875400201992916E-4 88 12.463934424688386 5.389820321565455E-4 3.133616466026427E-6 4.70042469903964E-5 2.318876184859556E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 229240 0.0 83.26046 8 AGTGGTA 230380 0.0 83.21819 5 GTGGTAT 230920 0.0 82.98236 6 TGGTATC 232130 0.0 82.24615 7 GTATCAA 232605 0.0 81.932724 9 GCAGTGG 236415 0.0 81.21314 3 AAGCAGT 233000 0.0 80.71082 1 CAGTGGT 243040 0.0 78.749916 4 AGCAGTG 247915 0.0 77.37768 2 CAACGCA 226880 0.0 43.110294 12-13 GAGTACT 218345 0.0 42.959976 20-21 ATCAACG 228165 0.0 42.927227 10-11 ACGCAGA 229330 0.0 42.70964 14-15 AGAGTAC 228950 0.0 42.457283 18-19 TCAACGC 226760 0.0 41.98295 12-13 AACGCAG 227430 0.0 41.92458 14-15 AGTACTT 230305 0.0 41.845383 20-21 CGCAGAG 230640 0.0 41.38999 16-17 TATCAAC 233620 0.0 40.766254 10-11 GTACTTT 231815 0.0 40.443027 22-23 >>END_MODULE