FastQCFastQC Report
Thu 26 May 2016
SRR1660748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30920493
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9856633.1877337790183353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337990.10930938261560061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1295250.081.7327968
GTGGTAT1319500.080.3018956
TGGTATC1322100.079.9023747
AGTGGTA1328050.079.802975
GTATCAA1344100.078.720369
GCAGTGG1388650.076.785913
AAGCAGT1407500.075.644341
CAGTGGT1442100.073.468884
AGCAGTG1478700.072.169862
CAACGCA1261250.043.55828512-13
ATCAACG1279800.043.0682310-11
ACGCAGA1285850.042.74016614-15
TCAACGC1258100.042.2523412-13
AGAGTAC1286250.042.0411218-19
AACGCAG1267500.042.00761414-15
GAGTACT1260050.041.20623420-21
CGCAGAG1298500.040.9366616-17
TATCAAC1328550.039.8226410-11
AGTACTT1367050.039.3106120-21
GTACTTT1371550.037.94454222-23
Produced by FastQC (version 0.11.5)