FastQCFastQC Report
Thu 26 May 2016
SRR1660748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30920493
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10881543.5192000334535414No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT326160.10548344103051656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1438850.081.921858
GTGGTAT1471850.080.458676
AGTGGTA1475550.080.3592155
TGGTATC1474350.079.9939357
GTATCAA1483600.079.374829
GCAGTGG1521300.077.9611053
AAGCAGT1517900.076.604761
CAGTGGT1574600.075.080374
AGCAGTG1626550.072.8528752
CAACGCA1395200.043.7614912-13
ATCAACG1413050.043.2702110-11
ACGCAGA1428300.042.8692414-15
TCAACGC1390000.042.36157612-13
AGAGTAC1448250.042.03256218-19
AACGCAG1405350.041.97731414-15
GAGTACT1410850.041.3433920-21
CGCAGAG1437900.041.14637816-17
TATCAAC1463100.040.2820310-11
AGTACTT1522300.039.74765820-21
GTACTTT1521450.038.3744222-23
Produced by FastQC (version 0.11.5)