FastQCFastQC Report
Thu 26 May 2016
SRR1660747_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660747_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30258098
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14923204.931968955880835No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT428310.1415521887727378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344960.11400584398926858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1922250.084.044488
AGTGGTA1948000.082.924155
GTGGTAT1953000.082.711576
TGGTATC1954050.082.4147347
GTATCAA1961900.082.2884759
AAGCAGT1999550.081.304541
GCAGTGG2021300.080.452363
CAGTGGT2054550.078.516084
AGCAGTG2105950.077.31282
CAACGCA1894750.044.36491812-13
ATCAACG1907050.044.1650610-11
ACGCAGA1918050.043.8173214-15
AGAGTAC1922500.043.19227618-19
TCAACGC1892850.042.86097712-13
AACGCAG1907950.042.56787514-15
GAGTACT1876400.042.26223420-21
CGCAGAG1931450.041.9511716-17
AGTACTT1978700.041.5769520-21
TATCAAC1969150.040.985610-11
GCAGAGT2071100.040.3973616-17
Produced by FastQC (version 0.11.5)