FastQCFastQC Report
Thu 26 May 2016
SRR1660747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30258098
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16265615.375622089663402No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT526260.17392368813135578No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT303270.10022771424694307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2077400.084.236628
AGTGGTA2120450.082.931345
GTGGTAT2123200.082.813516
GTATCAA2113150.082.682539
TGGTATC2119050.082.6674357
AAGCAGT2104800.081.794181
GCAGTGG2179450.080.744533
CAGTGGT2220400.079.003514
AGCAGTG2255050.077.920882
CAACGCA2043000.044.4607512-13
ATCAACG2057400.044.2214110-11
ACGCAGA2073550.043.94305414-15
AGAGTAC2097650.043.18486818-19
TCAACGC2038150.042.87994412-13
AACGCAG2057650.042.5294514-15
GAGTACT2044950.042.28463720-21
CGCAGAG2083000.042.1088916-17
AGTACTT2151750.041.7466220-21
TATCAAC2124200.041.14169710-11
GCAGAGT2244800.040.6828816-17
Produced by FastQC (version 0.11.5)