##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660747_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30258098 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01962800173362 33.0 31.0 34.0 27.0 34.0 2 31.809613512389312 34.0 31.0 34.0 28.0 34.0 3 32.31055940793106 34.0 31.0 34.0 30.0 34.0 4 35.83927783563924 37.0 35.0 37.0 35.0 37.0 5 35.89861923905462 37.0 35.0 37.0 35.0 37.0 6 35.916246454089745 37.0 35.0 37.0 35.0 37.0 7 35.90793767010735 37.0 35.0 37.0 35.0 37.0 8 35.91612648620544 37.0 35.0 37.0 35.0 37.0 9 37.62749449750609 39.0 37.0 39.0 35.0 39.0 10-11 37.57394747680439 39.0 37.0 39.0 35.0 39.0 12-13 37.636795478684746 39.0 37.0 39.0 35.0 39.0 14-15 38.966502107964615 40.0 38.0 41.0 35.0 41.0 16-17 38.98546023943739 40.0 38.0 41.0 35.0 41.0 18-19 38.98311977507641 40.0 38.5 41.0 35.0 41.0 20-21 38.92212765653677 40.0 38.5 41.0 35.0 41.0 22-23 38.81617745768422 40.0 38.0 41.0 34.5 41.0 24-25 38.77007113930294 40.0 38.0 41.0 34.0 41.0 26-27 38.73604226544576 40.0 38.0 41.0 34.0 41.0 28-29 38.6419529079455 40.0 38.0 41.0 34.0 41.0 30-31 38.54902198413132 40.0 38.0 41.0 34.0 41.0 32-33 38.44853409160087 40.0 38.0 41.0 34.0 41.0 34-35 38.34164419389481 40.0 38.0 41.0 34.0 41.0 36-37 38.15466416626716 40.0 38.0 41.0 33.5 41.0 38-39 37.73677644906828 40.0 36.0 41.0 33.0 41.0 40-41 37.59399283788426 40.0 36.0 41.0 33.0 41.0 42-43 37.43766741386058 40.0 36.0 41.0 33.0 41.0 44-45 37.327152519632925 40.0 35.0 41.0 32.0 41.0 46-47 37.198583731204785 39.0 35.0 41.0 32.0 41.0 48-49 37.32130461405737 40.0 35.0 41.0 32.0 41.0 50-51 37.230928609590734 40.0 35.0 41.0 32.0 41.0 52-53 36.970585543744356 39.0 35.0 41.0 31.0 41.0 54-55 36.47280109939494 39.0 35.0 41.0 29.5 41.0 56-57 36.1682125558586 39.0 35.0 41.0 28.0 41.0 58-59 35.98013887059259 39.0 35.0 41.0 28.0 41.0 60-61 35.79697269801955 39.0 35.0 41.0 27.0 41.0 62-63 35.52289744054633 38.5 35.0 41.0 26.5 41.0 64-65 35.287426096643614 38.0 35.0 40.0 26.0 41.0 66-67 35.028808915880965 37.0 34.0 40.0 26.0 41.0 68-69 34.742022119169555 37.0 34.0 40.0 26.0 41.0 70-71 34.418490316212214 36.0 34.0 39.0 26.0 41.0 72-73 34.104961422228186 36.0 34.0 39.0 26.0 41.0 74-75 33.75124490640489 35.0 34.0 38.5 25.5 40.0 76-77 31.652127407347283 34.0 30.5 36.0 21.5 39.0 78-79 32.919503681295495 35.0 33.0 37.0 24.5 39.0 80-81 32.90033780378397 35.0 33.0 36.5 25.0 39.0 82-83 32.672930202023934 35.0 33.0 36.0 25.0 38.0 84-85 32.430206270731226 35.0 33.0 36.0 24.0 37.0 86-87 32.21856443190844 35.0 33.0 35.5 24.0 37.0 88-89 31.998786490148852 35.0 33.0 35.0 23.5 36.5 90-91 31.836697319838144 35.0 33.0 35.0 23.0 36.0 92-93 31.707201473800502 35.0 33.0 35.0 23.0 36.0 94-95 31.607634144750275 35.0 33.0 35.0 21.0 36.0 96-97 31.50495668961083 35.0 33.0 35.0 20.0 35.0 98-99 31.393124329890135 35.0 32.5 35.0 20.0 35.0 100 31.210263381392974 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.7644880261856883 1101 2 1.2181447962226386 1101 3 0.7785279612309637 1101 4 0.6027277248270266 1101 5 0.47869441742170693 1101 6 0.4359267830049802 1101 7 0.39952546956244817 1101 8 0.4104004629329552 1101 9 0.5045594842790138 1101 10-11 0.5181522329206487 1101 12-13 0.4737721807472042 1101 14-15 0.6283537630197671 1101 16-17 0.6032647037716359 1101 18-19 0.5603517460321186 1101 20-21 0.5841019026254557 1101 22-23 0.5749780234822452 1101 24-25 0.6237804079839933 1101 26-27 0.5861507673994097 1101 28-29 0.6109175342610911 1101 30-31 0.6162163634298281 1101 32-33 0.604918365127638 1101 34-35 0.6170426779543092 1101 36-37 0.5938977419320253 1101 38-39 0.6786729739948569 1101 40-41 0.6665935312773712 1101 42-43 0.6242684064361228 1101 44-45 0.5976400759630991 1101 46-47 0.5834607089971797 1101 48-49 0.580587184673945 1101 50-51 0.5497408065399156 1101 52-53 0.5820234570004814 1101 54-55 0.6668242563081463 1101 56-57 0.7390429571016952 1101 58-59 0.7121904298444122 1101 60-61 0.6783368913284775 1101 62-63 0.6839497725954615 1101 64-65 0.6672727003177954 1101 66-67 0.5820393701532751 1101 68-69 0.582865037073681 1101 70-71 0.5651429043856311 1101 72-73 0.5433066129116639 1101 74-75 0.5453685215414765 1101 76-77 0.4786613144579448 1101 78-79 0.5714963788645164 1101 80-81 0.5198322872804937 1101 82-83 0.478150593111053 1101 84-85 0.49575018502993373 1101 86-87 0.49270600457396796 1101 88-89 0.517713684907541 1101 90-91 0.5359986799134759 1101 92-93 0.4886880091460739 1101 94-95 0.4335668414710909 1101 96-97 0.4841026787419942 1101 98-99 0.4983949080456149 1101 100 0.41600349413706184 1102 1 1.675643788917327 1102 2 1.1486432266332756 1102 3 0.7128640872576639 1102 4 0.5501453111177383 1102 5 0.41698571786036354 1102 6 0.39140261524296704 1102 7 0.356650582697732 1102 8 0.376158556368722 1102 9 0.465755666936019 1102 10-11 0.44643792409517147 1102 12-13 0.39808443298715446 1102 14-15 0.5691315930031422 1102 16-17 0.5372640203349803 1102 18-19 0.5228553848821278 1102 20-21 0.5066347256830923 1102 22-23 0.49872899495181855 1102 24-25 0.5507234165162203 1102 26-27 0.5220008931481743 1102 28-29 0.5359333017305374 1102 30-31 0.5514318227314234 1102 32-33 0.5077429799611508 1102 34-35 0.4914100851637997 1102 36-37 0.5384750005175007 1102 38-39 0.5759373872611846 1102 40-41 0.5539022228019235 1102 42-43 0.5668556865987497 1102 44-45 0.5808366968390146 1102 46-47 0.5704665987456536 1102 48-49 0.5195022989419016 1102 50-51 0.517235897218562 1102 52-53 0.44133465793490245 1102 54-55 0.5009708120042546 1102 56-57 0.5532054008049272 1102 58-59 0.5354601544615392 1102 60-61 0.49449881275344154 1102 62-63 0.5404570466532945 1102 64-65 0.5126804578634463 1102 66-67 0.4553549476973302 1102 68-69 0.45490287649238326 1102 70-71 0.4973116551893568 1102 72-73 0.434321266672157 1102 74-75 0.4110692803258047 1102 76-77 0.35232415111682513 1102 78-79 0.4739629263480012 1102 80-81 0.4806392797732997 1102 82-83 0.4372816511561268 1102 84-85 0.4451321282660814 1102 86-87 0.42290700069282394 1102 88-89 0.44519292970736046 1102 90-91 0.4257615579975891 1102 92-93 0.3782302906001007 1102 94-95 0.3477488727229989 1102 96-97 0.37460493042948073 1102 98-99 0.4251396035670929 1102 100 0.3506984589267468 1103 1 1.6533176545119517 1103 2 1.0969729301331057 1103 3 0.6606820754466582 1103 4 0.5424967503469702 1103 5 0.41179983937681897 1103 6 0.3636076176235008 1103 7 0.321297846145292 1103 8 0.3674752001329935 1103 9 0.4369908463460348 1103 10-11 0.3953842158105303 1103 12-13 0.37543628495361503 1103 14-15 0.5299581888933673 1103 16-17 0.49534489942569593 1103 18-19 0.4222996849329306 1103 20-21 0.44228960550850616 1103 22-23 0.429441294343647 1103 24-25 0.5103277906169978 1103 26-27 0.4758287395003862 1103 28-29 0.49157606948186583 1103 30-31 0.5038766953627061 1103 32-33 0.46826734682102966 1103 34-35 0.49234076175174124 1103 36-37 0.5013009625359501 1103 38-39 0.5356485153533228 1103 40-41 0.5258488485701278 1103 42-43 0.49184002200554033 1103 44-45 0.49916962131146647 1103 46-47 0.5199159364447823 1103 48-49 0.4905262961729022 1103 50-51 0.44939989106020306 1103 52-53 0.4309674131862735 1103 54-55 0.4929004397848331 1103 56-57 0.5269760301560211 1103 58-59 0.5107138580271382 1103 60-61 0.48163122349514254 1103 62-63 0.5196583300604445 1103 64-65 0.4834569694030719 1103 66-67 0.4574747624464308 1103 68-69 0.5235820090579821 1103 70-71 0.5114467032517354 1103 72-73 0.4518451813039306 1103 74-75 0.4058978631108374 1103 76-77 0.2550753251916831 1103 78-79 0.39260613918539633 1103 80-81 0.4090299451899071 1103 82-83 0.3960516259235689 1103 84-85 0.4133230689277525 1103 86-87 0.35554642890067356 1103 88-89 0.4245700894075881 1103 90-91 0.4161690069977624 1103 92-93 0.35928004300064487 1103 94-95 0.33826979226920884 1103 96-97 0.3544692477678524 1103 98-99 0.3933996041373362 1103 100 0.3079053688247413 1104 1 1.582678182445143 1104 2 1.0601237297303072 1104 3 0.6129602933707119 1104 4 0.504338772053373 1104 5 0.3601944255589089 1104 6 0.3333410443341194 1104 7 0.2920028034811324 1104 8 0.2916139753482767 1104 9 0.3818244550466048 1104 10-11 0.3727694410024398 1104 12-13 0.3418158673423477 1104 14-15 0.48872071253033766 1104 16-17 0.47359230494122784 1104 18-19 0.39189267173362197 1104 20-21 0.3826421743074988 1104 22-23 0.3983879179777787 1104 24-25 0.45761832732618046 1104 26-27 0.46138562495666946 1104 28-29 0.452881635565177 1104 30-31 0.42423705283223967 1104 32-33 0.4387267325280675 1104 34-35 0.39495479456797966 1104 36-37 0.45499761532243355 1104 38-39 0.47648815468244976 1104 40-41 0.47829866707009927 1104 42-43 0.46306285524907054 1104 44-45 0.484756018147408 1104 46-47 0.5281188134733128 1104 48-49 0.5147286680186767 1104 50-51 0.46305657277539325 1104 52-53 0.45449474821855773 1104 54-55 0.5250012924845606 1104 56-57 0.57961580829447 1104 58-59 0.5248353729560975 1104 60-61 0.4910361684669411 1104 62-63 0.560230055727807 1104 64-65 0.5516324525710417 1104 66-67 0.5524991909574979 1104 68-69 0.5176611683692371 1104 70-71 0.5173779295825156 1104 72-73 0.4836079527452384 1104 74-75 0.45912660102382574 1104 76-77 0.43694422689938506 1104 78-79 0.5030361997587178 1104 80-81 0.4650612992824037 1104 82-83 0.4397197052918642 1104 84-85 0.4819902862541028 1104 86-87 0.46784613317353774 1104 88-89 0.5189570033041448 1104 90-91 0.5286794571527054 1104 92-93 0.5258040108708997 1104 94-95 0.5369503370258712 1104 96-97 0.5012200340211628 1104 98-99 0.5504801623874762 1104 100 0.4773276301810512 1105 1 1.5886744288510783 1105 2 1.0500792809388315 1105 3 0.624470991151 1105 4 0.503136365747828 1105 5 0.37248887742578773 1105 6 0.30926094335212895 1105 7 0.2510811821221637 1105 8 0.2943784413089858 1105 9 0.3432003014243463 1105 10-11 0.3324687588493802 1105 12-13 0.2931458278034995 1105 14-15 0.40528459684604456 1105 16-17 0.34014140291382944 1105 18-19 0.32600217936502673 1105 20-21 0.34635250950902474 1105 22-23 0.33878448172326614 1105 24-25 0.36976190779876106 1105 26-27 0.33001453713736595 1105 28-29 0.3436241546255161 1105 30-31 0.36211492459133154 1105 32-33 0.3842981501001361 1105 34-35 0.3733980469272211 1105 36-37 0.43113357959001775 1105 38-39 0.45315637962992383 1105 40-41 0.42402293499031174 1105 42-43 0.40701217365992903 1105 44-45 0.4313025390616758 1105 46-47 0.4690270263154517 1105 48-49 0.4432266671707765 1105 50-51 0.42290011356890034 1105 52-53 0.37963202796721873 1105 54-55 0.48083753082213576 1105 56-57 0.5047251954910621 1105 58-59 0.5101735586098286 1105 60-61 0.4567433425048151 1105 62-63 0.5183769284726409 1105 64-65 0.4550356090233407 1105 66-67 0.47493359183526707 1105 68-69 0.5324152738205967 1105 70-71 0.49873604374321445 1105 72-73 0.42285233631262287 1105 74-75 0.38469646008255154 1105 76-77 0.3667184094304119 1105 78-79 0.48421226760720515 1105 80-81 0.44868410984380347 1105 82-83 0.4600274958657167 1105 84-85 0.49291398818886023 1105 86-87 0.46535330711374456 1105 88-89 0.4944375687566023 1105 90-91 0.5184656054943844 1105 92-93 0.46565484379536315 1105 94-95 0.43929676235969595 1105 96-97 0.4278437627380818 1105 98-99 0.4736762593417012 1105 100 0.45249855679583817 1106 1 1.3991708798342017 1106 2 0.8654535094616023 1106 3 0.4684185371341627 1106 4 0.4116460332914187 1106 5 0.23140306505767683 1106 6 0.20729387995778126 1106 7 0.16122721248133587 1106 8 0.1827066604411769 1106 9 0.22162521942710356 1106 10-11 0.20540619654099856 1106 12-13 0.19147001626600257 1106 14-15 0.3233317824823203 1106 16-17 0.250302130625748 1106 18-19 0.19713584015074304 1106 20-21 0.20023166806898018 1106 22-23 0.1772205549541752 1106 24-25 0.20799936284753784 1106 26-27 0.21171748700978554 1106 28-29 0.25869730795287893 1106 30-31 0.22413079096516952 1106 32-33 0.21987505846195177 1106 34-35 0.2325060190794872 1106 36-37 0.22382409277876292 1106 38-39 0.2976566634651263 1106 40-41 0.2666804424693012 1106 42-43 0.24438025688095166 1106 44-45 0.21891811310083398 1106 46-47 0.22559819956349259 1106 48-49 0.2424462964346219 1106 50-51 0.22938615308630972 1106 52-53 0.18709506704900036 1106 54-55 0.22482836246201288 1106 56-57 0.2011637662491026 1106 58-59 0.13782062714891197 1106 60-61 0.16403839738315185 1106 62-63 0.2150768072003828 1106 64-65 0.20641635097435795 1106 66-67 0.18980705041182233 1106 68-69 0.17595374765767957 1106 70-71 0.20379137721551643 1106 72-73 0.1590299497140606 1106 74-75 0.10911982624683247 1106 76-77 0.06428683783184397 1106 78-79 0.21153884646218302 1106 80-81 0.1899477032119421 1106 82-83 0.14482704071949115 1106 84-85 0.16542717625230807 1106 86-87 0.13356361804447658 1106 88-89 0.1575402809526949 1106 90-91 0.18420173113513272 1106 92-93 0.12019271522008168 1106 94-95 0.14807737628083117 1106 96-97 0.15091028444553345 1106 98-99 0.2148355448788486 1106 100 0.1536774251798647 1107 1 1.5671337813606954 1107 2 1.0207148586966 1107 3 0.6069390684045146 1107 4 0.4957386781754991 1107 5 0.33511908017480607 1107 6 0.3205929328029171 1107 7 0.2607990513491245 1107 8 0.280172005838466 1107 9 0.3463426196122512 1107 10-11 0.29591302969286204 1107 12-13 0.2938038800106497 1107 14-15 0.42216799193872845 1107 16-17 0.38183487006260464 1107 18-19 0.3248698984852112 1107 20-21 0.3347081627856312 1107 22-23 0.34060907145759245 1107 24-25 0.3884173034272891 1107 26-27 0.337233057061205 1107 28-29 0.3223195483633461 1107 30-31 0.3704129970260084 1107 32-33 0.3898235113046553 1107 34-35 0.37144967710518983 1107 36-37 0.4432252375700614 1107 38-39 0.4374874657284167 1107 40-41 0.4227964402790647 1107 42-43 0.3786753139683654 1107 44-45 0.3878809698950505 1107 46-47 0.4376075203780161 1107 48-49 0.40923040897568796 1107 50-51 0.41178095492651323 1107 52-53 0.39488210535752444 1107 54-55 0.44600582518256005 1107 56-57 0.35746785785384105 1107 58-59 0.37109608474045785 1107 60-61 0.3238725258218338 1107 62-63 0.3731595877577476 1107 64-65 0.373018906115135 1107 66-67 0.3748913180466644 1107 68-69 0.3535292282221931 1107 70-71 0.36607281654639223 1107 72-73 0.38625083386189374 1107 74-75 0.34832037589485765 1107 76-77 0.2678588042957344 1107 78-79 0.376878340369295 1107 80-81 0.36329752771894874 1107 82-83 0.34485534084646474 1107 84-85 0.3216819465783374 1107 86-87 0.3055378453175308 1107 88-89 0.3413664223651196 1107 90-91 0.37619453543182857 1107 92-93 0.37636372387747485 1107 94-95 0.3706317936406691 1107 96-97 0.31922662593258977 1107 98-99 0.3359081475932264 1107 100 0.30139063811554223 1108 1 1.4248595588871353 1108 2 0.901528935398531 1108 3 0.4640554085337385 1108 4 0.39498431779558985 1108 5 0.2340826586005349 1108 6 0.1783095840785407 1108 7 0.14180509129038654 1108 8 0.1405639007670274 1108 9 0.16887437945658235 1108 10-11 0.15235091288268876 1108 12-13 0.14743694858113798 1108 14-15 0.20031314648159793 1108 16-17 0.16373636999942676 1108 18-19 0.13642272410110223 1108 20-21 0.15979874408367323 1108 22-23 0.1068321500540037 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48-49 -1.7011498952570037 2316 50-51 -1.5105194273305855 2316 52-53 -1.3274385084765754 2316 54-55 -1.2982661558749768 2316 56-57 -1.4394253884553052 2316 58-59 -1.259424767598233 2316 60-61 -1.201396537214407 2316 62-63 -2.279774186182898 2316 64-65 -1.6272212062746192 2316 66-67 -1.3386353452646276 2316 68-69 -1.268037510611478 2316 70-71 -1.1337456151635408 2316 72-73 -1.0702212356533067 2316 74-75 -1.236745560637317 2316 76-77 -1.0562268231099239 2316 78-79 -1.3682046156746566 2316 80-81 -1.2146504589317217 2316 82-83 -1.0919508707817087 2316 84-85 -1.0061774586150563 2316 86-87 -1.185146927288848 2316 88-89 -1.1563151458213525 2316 90-91 -1.1185438611975371 2316 92-93 -1.048844646499692 2316 94-95 -1.0571337042787512 2316 96-97 -1.0068617457863347 2316 98-99 -1.0399178072238762 2316 100 -1.0026743109080734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 56.0 7 834.0 8 1067.0 9 434.0 10 218.0 11 181.0 12 229.0 13 664.0 14 2714.0 15 8124.0 16 18553.0 17 30881.0 18 41975.0 19 54773.0 20 75865.0 21 109757.0 22 167536.0 23 271280.0 24 439278.0 25 545671.0 26 523648.0 27 447198.0 28 421870.0 29 461171.0 30 539585.0 31 652402.0 32 801592.0 33 1006567.0 34 1341861.0 35 1956807.0 36 3286892.0 37 6202484.0 38 8267487.0 39 2572663.0 40 5781.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.19034622926296 33.29342601509409 26.87324314391898 24.64298461172397 2 20.104581590025916 32.29913856449272 30.494666254303226 17.101613591178136 3 24.116439837031393 26.644632455086896 27.45544680303435 21.78348090484736 4 18.210460551750476 28.73369634799914 26.304888694590122 26.750954405660266 5 21.22517242507644 32.96863312947836 26.87033654082922 18.935857904615972 6 26.875512796607374 27.688075436863212 26.943825087750064 18.492586678779347 7 20.24188037199166 27.0871784472375 32.87543387558597 19.795507305184877 8 25.384837473921856 26.67846802532003 27.663149878092142 20.273544622665973 9 25.108785753817042 25.21346186399423 29.30505083300345 20.37270154918528 10-11 19.929099641358818 29.9310601082725 30.035152903530154 20.104687346838524 12-13 19.938576443238436 27.08381240618627 29.628843491748885 23.348767658826407 14-15 19.952167846108505 33.195655589455754 26.574089356178305 20.27808720825744 16-17 23.20180700055899 26.852475988411435 26.601072215444603 23.34464479558497 18-19 20.05849976455042 30.008744278654515 26.620401984984472 23.312353971810595 20-21 23.171291909171096 30.094526569591963 26.58856086646028 20.145620654776664 22-23 23.13094497876238 27.03432813258784 29.679560493194252 20.155166395455524 24-25 20.172438443334897 29.924668710348257 26.432527573375104 23.470365272941745 26-27 20.172155479582425 26.876895268117178 32.75703984350127 20.19390940879913 28-29 20.177048140963784 26.98749108420496 32.695085461088794 20.140375313742457 30-31 20.135330383291112 26.925040033910925 32.67184044416804 20.26778913862993 32-33 20.251606693851016 27.05167059740503 32.48466575790719 20.212056950836764 34-35 20.273964345016 27.12557147511387 32.39434448259107 20.206119697279057 36-37 20.22715373583627 27.108409127368148 32.42025985903014 20.244177277765445 38-39 20.2595487042766 27.099497911261604 32.461480765603326 20.179472618858473 40-41 20.27039177939133 27.136395776745285 32.425756420731 20.167456023132385 42-43 20.29308442672785 27.16206491039158 32.34915070198975 20.195699960890824 44-45 20.317535775384556 27.235467271861886 32.320314241928386 20.126682710825172 46-47 20.366856141952496 27.3291652439068 32.21934162294387 20.08463699119682 48-49 20.40250319271042 27.36767592444589 32.08478029080861 20.145040592035087 50-51 20.453061854714065 27.455973934647183 31.974522985549193 20.116441225089563 52-53 20.572820586537766 27.613316574390634 31.653198817166285 20.160664021905315 54-55 20.884019870992788 27.841210522460358 30.910022681383026 20.36474692516383 56-57 21.291054878226017 28.400136711682165 29.78168285219713 20.527125557894692 58-59 21.397477854641153 28.933072592041874 29.06952205293728 20.599927500379692 60-61 21.441420444844795 29.204981860342848 28.68810061360934 20.665497081203014 62-63 21.463973510614455 29.33463908139487 28.49820757358883 20.703179834401848 64-65 21.4580419579264 29.440592598213378 28.36300580013803 20.73835964372219 66-67 21.399688740080453 29.346103491370208 28.44113760822492 20.813070160324422 68-69 21.37573436369743 29.24111337819488 28.550743545180126 20.83240871292757 70-71 21.320626504955374 29.202138465594356 28.553872622662528 20.923362406787742 72-73 21.256826476239585 29.121929492357012 28.55852821266465 21.06271581873875 74-75 21.306510388901582 29.13136173688456 28.438579915418217 21.123547958795644 76-77 21.341306599344207 29.17934106120452 28.27683510087834 21.202517238572927 78-79 21.377274115468467 29.30283145374915 27.946140192614628 21.373754238167756 80-81 21.407523160937966 29.43046734609373 27.66228892586898 21.499720567099317 82-83 21.39636984355181 29.532188357345067 27.340258808293843 21.731182990809277 84-85 21.380454221281052 29.543887609255627 27.143024374857205 21.932633794606122 86-87 21.38322949866371 29.539779494061502 27.000011732731032 22.076979274543756 88-89 21.426112558621785 29.501424155614014 26.838264146836355 22.234199138927845 90-91 21.389142389444014 29.398667598438198 26.75261921929497 22.459570792822824 92-93 21.38758046539398 29.313048814536224 26.7533348101555 22.546035909914288 94-95 21.37526761326414 29.234005552778996 26.737373502352263 22.653353331604603 96-97 21.36626643790408 29.11322460671095 26.749944792680175 22.770564162704794 98-99 21.37643462613084 29.003382998346922 26.79572662411932 22.824455751402915 100 21.357402999134774 28.94313467532003 26.74518949942498 22.954272826120214 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21369.0 1 16875.5 2 10088.0 3 7131.5 4 6470.0 5 6501.0 6 6700.0 7 7029.0 8 7278.5 9 7610.5 10 8596.5 11 11913.5 12 114063.0 13 139997.0 14 64704.0 15 67801.5 16 78170.0 17 89478.5 18 100620.5 19 111882.0 20 126567.5 21 148197.0 22 176550.5 23 208600.5 24 241213.5 25 277657.0 26 321307.0 27 370205.0 28 426923.5 29 485588.0 30 538191.0 31 589368.0 32 645911.0 33 702803.0 34 748601.0 35 797882.5 36 860388.0 37 909137.5 38 943501.5 39 969787.0 40 980771.0 41 990262.0 42 1007581.5 43 1011231.0 44 1008184.0 45 1014587.0 46 1012202.0 47 1004975.5 48 990614.0 49 969635.0 50 951516.5 51 930627.5 52 901249.0 53 855294.0 54 796841.0 55 734516.0 56 666571.5 57 592722.5 58 516423.0 59 438404.5 60 360039.0 61 289647.5 62 227454.5 63 170469.5 64 124648.0 65 90291.5 66 64188.0 67 47054.5 68 35081.0 69 26021.5 70 20000.5 71 15915.5 72 13129.5 73 10381.0 74 7963.5 75 6322.5 76 4798.0 77 3086.0 78 1869.5 79 1215.5 80 749.0 81 528.5 82 379.0 83 220.5 84 130.0 85 98.5 86 64.0 87 39.5 88 32.5 89 27.0 90 21.5 91 16.5 92 8.0 93 5.0 94 5.0 95 4.0 96 2.0 97 2.5 98 2.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10651693969660617 2 0.0 3 0.0 4 0.0 5 0.00967674835344905 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.088476083328172E-5 20-21 0.010985488909448308 22-23 0.0 24-25 9.452015126661298E-4 26-27 0.004940826088936588 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.6524501969687586E-6 40-41 0.007711985069253196 42-43 0.039662109627644146 44-45 0.01559747740918811 46-47 0.004856551128891181 48-49 0.0016507977467717897 50-51 0.0 52-53 0.0013930155160446637 54-55 7.436025886359413E-5 56-57 8.576216522267857E-4 58-59 2.8091653348468896E-5 60-61 2.776116330907514E-4 62-63 3.023983860452828E-4 64-65 8.262250984843793E-5 66-67 3.5197189195434553E-4 68-69 6.130590230754094E-4 70-71 4.659909555451899E-4 72-73 0.0011798494406356935 74-75 0.001718548204847509 76-77 0.002430754239741044 78-79 0.0044996218863459295 80-81 0.004235229854830928 82-83 0.007492209193056352 84-85 0.0024158821879683248 86-87 0.002852129039968077 88-89 0.005778618338799749 90-91 0.005796795290966405 92-93 0.005586934115951373 94-95 0.002696798721453014 96-97 0.0055968488171331855 98-99 0.00790862664269248 100 0.3018398578787074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0258098E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.709158617313236 #Duplication Level Percentage of deduplicated Percentage of total 1 73.16641850881179 32.71209010571189 2 14.185109746718116 12.684086433400326 3 4.806253620337815 6.44650666400158 4 2.230033190029913 3.9881163045968164 5 1.3500138085653401 3.017899075135547 6 0.8493664773208015 2.278467633525858 7 0.5977202404267182 1.8706498326611727 8 0.43261854522221993 1.5473608927305227 9 0.33968129297197697 1.366817832613635 >10 1.754671503707107 14.290013819866193 >50 0.17506988837770357 5.551966168963284 >100 0.11202910842672427 7.917240482190048 >500 5.291579627482916E-4 0.14748855716060494 >1k 4.336008639155185E-4 0.4160958260635873 >5k 1.4660073443778408E-5 0.03712737323881729 >10k+ 3.6650183479482695E-5 5.728072998140407 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1626561 5.375622089663402 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 52626 0.17392368813135578 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 30327 0.10022771424694307 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.9413613506043903E-4 9.914701181812552E-6 0.0 0.0 6.609800787875034E-6 2 3.0074593584831404E-4 1.9829402363625104E-5 0.0 0.0 9.914701181812552E-6 3 3.106606370301266E-4 2.6439203151500138E-5 0.0 6.609800787875034E-6 1.3219601575750069E-5 4 3.3379493978768923E-4 3.635390433331269E-5 0.0 9.914701181812552E-6 1.3219601575750069E-5 5 3.3379493978768923E-4 3.635390433331269E-5 0.0 9.914701181812552E-6 2.6439203151500138E-5 6 3.5692924254525187E-4 3.635390433331269E-5 0.0 1.6524501969687587E-5 3.3049003939375174E-5 7 3.6023414293918937E-4 3.965880472725021E-5 0.0 2.313430275756262E-5 3.3049003939375174E-5 8 3.7345374451493944E-4 4.957350590906276E-5 0.0 2.9744103545437654E-5 4.2963705121187725E-5 9 3.7675864490887694E-4 5.2878406303000275E-5 0.0 3.3049003939375174E-5 4.2963705121187725E-5 10-11 3.9658804727250207E-4 6.279310748481283E-5 0.0 3.3049003939375174E-5 4.626860551512524E-5 12-13 4.197223500300647E-4 7.931760945450042E-5 0.0 3.800635453028145E-5 4.626860551512524E-5 14-15 4.560762543633774E-4 9.5842111424188E-5 0.0 4.2963705121187725E-5 4.7921055712094E-5 16-17 4.8251545751487753E-4 1.0245191221206303E-4 0.0 4.626860551512524E-5 5.618330669693779E-5 18-19 5.353938638178778E-4 1.1732396398478185E-4 0.0 4.7921055712094E-5 5.783575689390655E-5 20-21 5.800100191360343E-4 1.2228131457568813E-4 0.0 5.4530856499969033E-5 6.609800787875035E-5 22-23 6.461080270147846E-4 1.2558621496962566E-4 0.0 5.948820709087531E-5 7.270780866662538E-5 24-25 8.890182059691921E-4 1.3219601575750067E-4 0.0 6.940290827268787E-5 8.262250984843793E-5 26-27 0.0017036761530747901 1.43763167136282E-4 0.0 6.940290827268787E-5 9.088476083328172E-5 28-29 0.004621903200921618 1.487205177271883E-4 0.0 7.105535846965662E-5 9.749456162115675E-5 30-31 0.012649506257795847 1.5863521890900083E-4 0.0 7.766515925753165E-5 1.0245191221206303E-4 32-33 0.029965531871831468 1.635925694999071E-4 0.0 8.592741024237545E-5 1.0575681260600055E-4 34-35 0.06278815013422193 1.718548204847509E-4 0.0 9.5842111424188E-5 1.0740926280296932E-4 36-37 0.11758174621550899 1.7350727068171965E-4 0.0 1.0079946201509428E-4 1.156715137878131E-4 38-39 0.2010717924173555 1.7681217107565715E-4 0.0 1.0245191221206303E-4 1.2889111536356317E-4 40-41 0.3161104177797296 1.8176952166656344E-4 0.0 1.0245191221206303E-4 1.4045826674234448E-4 42-43 0.46909921436568813 1.949891232423135E-4 0.0 1.0740926280296932E-4 1.4211071693931323E-4 44-45 0.6639396170902745 2.0986117501503235E-4 0.0 1.2062886437871938E-4 1.4541561733325076E-4 46-47 0.9070216508651667 2.1977587619684489E-4 0.0 1.3880581654537573E-4 1.4541561733325076E-4 48-49 1.2042230810409826 2.2803812718168867E-4 0.0 1.4706806753021952E-4 1.5863521890900083E-4 50-51 1.5519184318855732 2.3630037816653248E-4 0.0 1.586352189090008E-4 1.6524501969687587E-4 52-53 1.945966663205334 2.4291017895440752E-4 0.0 1.7185482048475087E-4 1.8342197186353221E-4 54-55 2.3920472463272477 2.4951997974228255E-4 0.0 1.7846462127262593E-4 1.8507442206050097E-4 56-57 2.894618491882735 2.5612978053015756E-4 0.0 1.8011707146959468E-4 1.9333667304534475E-4 58-59 3.453463928895993 2.610871311210639E-4 0.0 1.8176952166656344E-4 1.9829402363625103E-4 60-61 4.058131809871195 2.676969319089389E-4 0.0 1.883793224544385E-4 2.1647097580290736E-4 62-63 4.703461532843208 2.743067326968139E-4 0.0 2.032513742271573E-4 2.2308077659078242E-4 64-65 5.387080179329184 2.7761163309075146E-4 0.0 2.0820872481806357E-4 2.3630037816653248E-4 66-67 6.111539132433242 2.825689836816577E-4 0.0 2.0986117501503235E-4 2.4456262915137627E-4 68-69 6.87773402016214 2.9083123466650153E-4 0.0 2.2142832639381364E-4 2.4456262915137627E-4 70-71 7.679066608879381 2.9413613506043903E-4 0.0 2.313430275756262E-4 2.5117242993925133E-4 72-73 8.509229496183137 2.9413613506043903E-4 0.0 2.3795282836350126E-4 2.5447733033318883E-4 74-75 9.365782013132485 3.090081868331578E-4 0.0 2.4786752954531377E-4 2.5778223072712634E-4 76-77 10.250036205183815 3.189228880149704E-4 0.0 2.528248801362201E-4 2.627395813180326E-4 78-79 11.155464894059104 3.271851389998142E-4 0.0 2.5778223072712634E-4 2.710018323028764E-4 80-81 12.080658539740337 3.3544738998465796E-4 0.0 2.594346809240951E-4 2.7761163309075146E-4 82-83 13.019972372354667 3.470145413634393E-4 0.0 2.627395813180326E-4 2.7761163309075146E-4 84-85 13.972870998038278 3.6023414293918937E-4 0.0 2.643920315150014E-4 2.7761163309075146E-4 86-87 14.942079307165969 3.6353904333312687E-4 0.0 2.676969319089389E-4 2.7761163309075146E-4 88 15.676761969638672 3.6684394372706443E-4 0.0 2.676969319089389E-4 2.8091653348468897E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 207740 0.0 84.23662 8 AGTGGTA 212045 0.0 82.93134 5 GTGGTAT 212320 0.0 82.81351 6 GTATCAA 211315 0.0 82.68253 9 TGGTATC 211905 0.0 82.667435 7 AAGCAGT 210480 0.0 81.79418 1 GCAGTGG 217945 0.0 80.74453 3 CAGTGGT 222040 0.0 79.00351 4 AGCAGTG 225505 0.0 77.92088 2 CAACGCA 204300 0.0 44.46075 12-13 ATCAACG 205740 0.0 44.22141 10-11 ACGCAGA 207355 0.0 43.943054 14-15 AGAGTAC 209765 0.0 43.184868 18-19 TCAACGC 203815 0.0 42.879944 12-13 AACGCAG 205765 0.0 42.52945 14-15 GAGTACT 204495 0.0 42.284637 20-21 CGCAGAG 208300 0.0 42.10889 16-17 AGTACTT 215175 0.0 41.74662 20-21 TATCAAC 212420 0.0 41.141697 10-11 GCAGAGT 224480 0.0 40.68288 16-17 >>END_MODULE