FastQCFastQC Report
Thu 26 May 2016
SRR1660746_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660746_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34141453
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17556875.142390981426596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA586960.17192004101290007No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT417960.12242009735203713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2356200.084.4685068
GTGGTAT2373650.083.961376
AGTGGTA2375450.083.9149865
GTATCAA2381100.083.500719
TGGTATC2376600.083.47197
AAGCAGT2449400.081.635171
GCAGTGG2492450.080.353943
CAGTGGT2499450.079.631544
AGCAGTG2593600.077.2538152
CAACGCA2318150.044.29722612-13
ATCAACG2339250.044.0123610-11
ACGCAGA2351150.043.76014314-15
AGAGTAC2332750.043.2160318-19
TCAACGC2311650.043.08065812-13
AACGCAG2341450.042.65638714-15
GAGTACT2253300.042.5444420-21
AGTACTT2345800.042.0830820-21
CGCAGAG2378350.041.9030816-17
TATCAAC2381900.041.6791810-11
GTACTTT2336950.040.76290522-23
Produced by FastQC (version 0.11.5)