##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660746_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34141453 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77872781219944 34.0 31.0 34.0 30.0 34.0 2 31.897324756506407 34.0 31.0 34.0 30.0 34.0 3 31.96013145661961 34.0 31.0 34.0 30.0 34.0 4 35.35610139381004 37.0 35.0 37.0 33.0 37.0 5 35.32165792709525 37.0 35.0 37.0 33.0 37.0 6 35.39752321027462 37.0 35.0 37.0 33.0 37.0 7 35.35334615079212 37.0 35.0 37.0 33.0 37.0 8 35.3605254293073 37.0 35.0 37.0 33.0 37.0 9 37.02933129413092 39.0 37.0 39.0 33.0 39.0 10-11 36.964188811764984 39.0 37.0 39.0 33.0 39.0 12-13 36.98478540148833 39.0 37.0 39.0 33.0 39.0 14-15 38.34445949034448 40.0 38.0 41.0 33.0 41.0 16-17 38.318432229583195 40.0 38.0 41.0 33.0 41.0 18-19 38.27439195396868 40.0 38.0 41.0 33.0 41.0 20-21 38.14898677569464 40.0 38.0 41.0 33.0 41.0 22-23 37.97203282473069 40.0 38.0 41.0 32.5 41.0 24-25 37.95097891703672 40.0 38.0 41.0 32.5 41.0 26-27 37.928014311517444 40.0 38.0 41.0 32.0 41.0 28-29 37.83924122971568 40.0 38.0 41.0 32.0 41.0 30-31 37.74243793900629 40.0 38.0 41.0 32.0 41.0 32-33 37.611936067864484 40.0 38.0 41.0 31.5 41.0 34-35 37.45008418358762 40.0 37.0 41.0 31.0 41.0 36-37 37.25524814365692 40.0 37.0 41.0 30.5 41.0 38-39 36.83823071326226 39.0 35.0 41.0 30.0 41.0 40-41 36.76863735119885 39.0 35.0 41.0 30.0 41.0 42-43 36.77091156606603 39.0 35.0 41.0 30.0 41.0 44-45 36.766866000108436 39.5 35.0 41.0 30.0 41.0 46-47 36.85921845505521 40.0 35.0 41.0 30.0 41.0 48-49 36.744516482646475 40.0 35.0 41.0 30.0 41.0 50-51 35.36789040583598 38.5 34.0 40.0 28.0 40.5 52-53 35.53532159278634 38.5 34.5 39.5 28.0 40.5 54-55 35.94395633952662 39.0 35.0 41.0 26.5 41.0 56-57 35.597474776483594 39.0 35.0 41.0 24.5 41.0 58-59 35.4113626329846 39.0 35.0 41.0 24.5 41.0 60-61 35.17662722204588 39.0 35.0 41.0 24.0 41.0 62-63 34.93129940896189 38.0 34.5 40.5 24.0 41.0 64-65 34.66482002098739 37.0 34.0 40.0 23.0 41.0 66-67 34.388775486503164 37.0 34.0 40.0 23.0 41.0 68-69 34.105229938514924 36.0 34.0 39.5 23.0 41.0 70-71 33.80387786952125 36.0 33.5 39.0 23.0 41.0 72-73 33.461165976152216 35.0 33.0 39.0 23.0 41.0 74-75 33.085643206222066 35.0 33.0 37.5 22.0 40.0 76-77 32.70396639240866 35.0 33.0 37.0 20.5 39.0 78-79 32.3440637104695 35.0 32.5 36.5 20.0 39.0 80-81 32.037978861063706 35.0 32.0 36.0 20.0 38.5 82-83 31.743648212628795 35.0 32.0 36.0 19.5 37.0 84-85 31.474984192969174 35.0 32.0 35.0 19.0 37.0 86-87 31.274394355741098 35.0 32.0 35.0 18.0 36.5 88-89 31.077900990329848 35.0 31.0 35.0 18.0 36.0 90-91 30.91567715351775 35.0 31.0 35.0 18.0 36.0 92-93 30.770962969853684 35.0 31.0 35.0 17.0 36.0 94-95 30.59492715497492 35.0 31.0 35.0 16.5 35.0 96-97 30.463367083410304 35.0 31.0 35.0 15.0 35.0 98-99 30.36266473486058 34.0 30.5 35.0 15.0 35.0 100 30.284428345800045 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5292634457202006 1101 2 0.5402494551118444 1101 3 0.5299627894531618 1101 4 0.4750131299367908 1101 5 0.484916992725104 1101 6 0.4378962375435549 1101 7 0.49267662831006476 1101 8 0.5068962766669571 1101 9 0.5722998838289826 1101 10-11 0.5823113017937445 1101 12-13 0.5827576537069064 1101 14-15 0.6949230377384765 1101 16-17 0.653247077159115 1101 18-19 0.6789323798980504 1101 20-21 0.7038529557670827 1101 22-23 0.7443661469915455 1101 24-25 0.7445833258359116 1101 26-27 0.703574559775042 1101 28-29 0.6836163732838685 1101 30-31 0.6493127920865831 1101 32-33 0.6529153182802361 1101 34-35 0.6721833265016954 1101 36-37 0.6240872893838372 1101 38-39 0.6171657179174588 1101 40-41 0.6279178124613551 1101 42-43 0.6420829443869351 1101 44-45 0.659148550192036 1101 46-47 0.6490615457730726 1101 48-49 0.7221609268340856 1101 50-51 0.6567596556142732 1101 52-53 0.7070293731594859 1101 54-55 0.7406900457193402 1101 56-57 0.7432556360414964 1101 58-59 0.6546063859726488 1101 60-61 0.6743493965952325 1101 62-63 0.634905110488738 1101 64-65 0.5745207044375036 1101 66-67 0.5781771699385629 1101 68-69 0.5722350087206962 1101 70-71 0.5430158213716894 1101 72-73 0.5164566285157051 1101 74-75 0.4799203302774373 1101 76-77 0.4811658762233648 1101 78-79 0.49133906656554416 1101 80-81 0.4581918160141001 1101 82-83 0.45809575386783763 1101 84-85 0.4234498857864182 1101 86-87 0.43219050450947805 1101 88-89 0.43722953684974186 1101 90-91 0.3843772035948412 1101 92-93 0.37763026097402275 1101 94-95 0.4047316246735768 1101 96-97 0.4133502030885232 1101 98-99 0.41126228813214283 1101 100 0.3580678415254148 1102 1 0.4592918226846514 1102 2 0.4845730560580854 1102 3 0.4631715962817289 1102 4 0.4173275026890835 1102 5 0.4227024175434053 1102 6 0.38972478330762783 1102 7 0.4068227541189984 1102 8 0.42170470335265975 1102 9 0.4808174201434028 1102 10-11 0.4835464454689671 1102 12-13 0.4894787168703516 1102 14-15 0.5934295947203765 1102 16-17 0.5763846111264499 1102 18-19 0.5449623932818 1102 20-21 0.5602427871411564 1102 22-23 0.5868358549928487 1102 24-25 0.5548259964213287 1102 26-27 0.5515234340692103 1102 28-29 0.599773251758073 1102 30-31 0.5485718369934958 1102 32-33 0.5583931463148986 1102 34-35 0.5434517172457092 1102 36-37 0.5983285362756234 1102 38-39 0.5993708248941445 1102 40-41 0.5211635939850083 1102 42-43 0.5420642053953202 1102 44-45 0.5273108143545571 1102 46-47 0.5893124377860772 1102 48-49 0.6285132505233904 1102 50-51 0.5933433499422662 1102 52-53 0.6416160938419466 1102 54-55 0.6863016429737385 1102 56-57 0.7175002313926484 1102 58-59 0.5997032096548551 1102 60-61 0.6024559173319801 1102 62-63 0.5696611125189932 1102 64-65 0.5346188512019978 1102 66-67 0.5096456276720218 1102 68-69 0.4998675495623388 1102 70-71 0.5332219385113461 1102 72-73 0.505141939815168 1102 74-75 0.49877388046894566 1102 76-77 0.47549338908307703 1102 78-79 0.4888352881183451 1102 80-81 0.45044170581790155 1102 82-83 0.4507881086550256 1102 84-85 0.414170393817205 1102 86-87 0.3927026996866694 1102 88-89 0.376511148984644 1102 90-91 0.34919268305516127 1102 92-93 0.37753424054063345 1102 94-95 0.4251031943868959 1102 96-97 0.3653072058838056 1102 98-99 0.30006298015058164 1102 100 0.307020418985541 1103 1 0.4145827811334506 1103 2 0.4329310629836165 1103 3 0.44609719091139866 1103 4 0.3774755913111889 1103 5 0.40221503370728584 1103 6 0.3824323318491878 1103 7 0.3666295828682067 1103 8 0.40196540445485596 1103 9 0.4937475709451036 1103 10-11 0.46293483807136937 1103 12-13 0.48470420830417993 1103 14-15 0.5791433224705571 1103 16-17 0.5386641213743957 1103 18-19 0.5299407087990815 1103 20-21 0.5616153474987797 1103 22-23 0.5846639798567637 1103 24-25 0.6310470378178294 1103 26-27 0.5623852855411968 1103 28-29 0.5684755435005329 1103 30-31 0.5564078049110464 1103 32-33 0.5277146323651678 1103 34-35 0.5010909172164304 1103 36-37 0.5022636841064525 1103 38-39 0.5015777109542938 1103 40-41 0.4724354436445637 1103 42-43 0.5045323311375469 1103 44-45 0.5275667027026856 1103 46-47 0.5433123251993095 1103 48-49 0.5879857279545746 1103 50-51 0.4872545043637402 1103 52-53 0.4936610051943333 1103 54-55 0.5420378238793191 1103 56-57 0.5728572716606806 1103 58-59 0.49251240138490715 1103 60-61 0.5365054114356695 1103 62-63 0.512995666288667 1103 64-65 0.49150353033062544 1103 66-67 0.4632718800540587 1103 68-69 0.44910224927397735 1103 70-71 0.4637939860382474 1103 72-73 0.41459694796896684 1103 74-75 0.42744111920465855 1103 76-77 0.4277083052489772 1103 78-79 0.3847393444951308 1103 80-81 0.3316058441412295 1103 82-83 0.33402268218324593 1103 84-85 0.32711028919586127 1103 86-87 0.30143204064622253 1103 88-89 0.2368755341816744 1103 90-91 0.29309696608748226 1103 92-93 0.2149884802087456 1103 94-95 0.3087804890721948 1103 96-97 0.2832408714668979 1103 98-99 0.27483946655677727 1103 100 0.2861827158416794 1104 1 0.39203701862585305 1104 2 0.429070063795141 1104 3 0.40684806807271556 1104 4 0.361864288684032 1104 5 0.3587533638309921 1104 6 0.3183399238227338 1104 7 0.31617561708820574 1104 8 0.3389284179516707 1104 9 0.4354702105989219 1104 10-11 0.3859241525035486 1104 12-13 0.4035979584185583 1104 14-15 0.49913409420715027 1104 16-17 0.4719515186899059 1104 18-19 0.45062142411954653 1104 20-21 0.3887890233347804 1104 22-23 0.43090261690318954 1104 24-25 0.4420774235587501 1104 26-27 0.4151736993297277 1104 28-29 0.42229984297367196 1104 30-31 0.38488401226067026 1104 32-33 0.3431165996206573 1104 34-35 0.37440719589226745 1104 36-37 0.3677383009103323 1104 38-39 0.3417498595977193 1104 40-41 0.34456394765115306 1104 42-43 0.3491795018324737 1104 44-45 0.36873836773902013 1104 46-47 0.38746316673451986 1104 48-49 0.44108944456057486 1104 50-51 0.37018351347721534 1104 52-53 0.38621425878842075 1104 54-55 0.4877177664104124 1104 56-57 0.5231676596155523 1104 58-59 0.4743876175379427 1104 60-61 0.4776988524489951 1104 62-63 0.4605743302604921 1104 64-65 0.4324695646731982 1104 66-67 0.4654892247565172 1104 68-69 0.4716970275033532 1104 70-71 0.44347064560622584 1104 72-73 0.4040136904645095 1104 74-75 0.38364611259788006 1104 76-77 0.36751583950621836 1104 78-79 0.39557094918767177 1104 80-81 0.38056054775788084 1104 82-83 0.350479216701288 1104 84-85 0.33128800452465157 1104 86-87 0.3130615373975729 1104 88-89 0.3151919204342839 1104 90-91 0.283106106958261 1104 92-93 0.300443283523002 1104 94-95 0.3932448685866028 1104 96-97 0.33741550687563304 1104 98-99 0.37464750554747894 1104 100 0.30854289436501503 1105 1 0.31067861193193735 1105 2 0.35662302289579983 1105 3 0.34399064906962806 1105 4 0.3010928824847241 1105 5 0.3180582850052289 1105 6 0.26956782224831954 1105 7 0.2895879397326908 1105 8 0.3112429610301177 1105 9 0.3262339950416262 1105 10-11 0.33649628316388913 1105 12-13 0.3369609239031135 1105 14-15 0.40803069887003574 1105 16-17 0.3556509902852625 1105 18-19 0.3904905495450137 1105 20-21 0.4056928680476872 1105 22-23 0.42930386628820116 1105 24-25 0.46607505145184547 1105 26-27 0.44192844236933126 1105 28-29 0.4385058391045433 1105 30-31 0.4638160340771549 1105 32-33 0.4270617847553808 1105 34-35 0.43745482985007555 1105 36-37 0.4122720830674069 1105 38-39 0.42533866760473416 1105 40-41 0.41003502442519135 1105 42-43 0.4403048395824172 1105 44-45 0.44495089140875166 1105 46-47 0.4517103796945179 1105 48-49 0.47001345400633454 1105 50-51 0.3606966502757203 1105 52-53 0.42716869050511974 1105 54-55 0.4681906045439561 1105 56-57 0.49490737124357054 1105 58-59 0.4667534362734429 1105 60-61 0.4678352190854014 1105 62-63 0.4758416325827426 1105 64-65 0.4560543819032574 1105 66-67 0.4026348507717259 1105 68-69 0.40962762236892303 1105 70-71 0.40318358215491656 1105 72-73 0.3818482525347946 1105 74-75 0.37892770634656614 1105 76-77 0.38027706616570356 1105 78-79 0.3745085672485544 1105 80-81 0.32828624373977533 1105 82-83 0.33060808529029373 1105 84-85 0.34143122209562193 1105 86-87 0.29242129353029256 1105 88-89 0.3280264485136648 1105 90-91 0.31999009353144103 1105 92-93 0.28630995344331467 1105 94-95 0.37450671249233736 1105 96-97 0.3493694591599059 1105 98-99 0.303275038693819 1105 100 0.3131825148488012 1106 1 0.2336218830463288 1106 2 0.2750955126323156 1106 3 0.25788462116272015 1106 4 0.25732774014259974 1106 5 0.2727768293787207 1106 6 0.2348966891913875 1106 7 0.23410917198908976 1106 8 0.266653075406893 1106 9 0.25479784235641745 1106 10-11 0.25043288198952496 1106 12-13 0.26339628694918815 1106 14-15 0.3357110819593885 1106 16-17 0.30389259187457895 1106 18-19 0.2917821267273979 1106 20-21 0.3370150052981131 1106 22-23 0.31177537048387904 1106 24-25 0.3125673235068973 1106 26-27 0.3628897978298298 1106 28-29 0.3485199285076561 1106 30-31 0.31823388662456153 1106 32-33 0.32857718220594734 1106 34-35 0.34603993451658255 1106 36-37 0.29432358695957817 1106 38-39 0.3045416039647364 1106 40-41 0.34999574780827203 1106 42-43 0.3300564717008925 1106 44-45 0.36640918200168215 1106 46-47 0.38103814891744037 1106 48-49 0.3747852152154394 1106 50-51 0.24588015424530596 1106 52-53 0.29813017288314114 1106 54-55 0.4432466419618848 1106 56-57 0.4607688008131987 1106 58-59 0.40123632623990346 1106 60-61 0.38734316663520474 1106 62-63 0.35255745819143414 1106 64-65 0.32926054754814515 1106 66-67 0.35776244036390636 1106 68-69 0.3543989567709289 1106 70-71 0.37550945332068153 1106 72-73 0.34902836875658494 1106 74-75 0.3415188886763403 1106 76-77 0.3336373208329988 1106 78-79 0.3305707593457754 1106 80-81 0.29420759114604067 1106 82-83 0.30184943122154095 1106 84-85 0.2725433774996944 1106 86-87 0.2820311442276555 1106 88-89 0.3021573745478108 1106 90-91 0.30395592208167344 1106 92-93 0.2572640100198811 1106 94-95 0.29613852618405545 1106 96-97 0.3051456667497483 1106 98-99 0.29815471090536505 1106 100 0.2927922679548516 1107 1 0.19940899415390945 1107 2 0.2262625424558884 1107 3 0.2105258454756047 1107 4 0.20254941613325173 1107 5 0.22309355999276903 1107 6 0.18869412508411187 1107 7 0.21129659562815561 1107 8 0.227725003603247 1107 9 0.22698337205099506 1107 10-11 0.20862852573501556 1107 12-13 0.2069910919560698 1107 14-15 0.24755147195155303 1107 16-17 0.2384964264776812 1107 18-19 0.22521837931505928 1107 20-21 0.22765933083559986 1107 22-23 0.2336825401486351 1107 24-25 0.2600100225392836 1107 26-27 0.2444435287112583 1107 28-29 0.26164690277444436 1107 30-31 0.21573203955676235 1107 32-33 0.19025564591983368 1107 34-35 0.21519063405714434 1107 36-37 0.21623865109185658 1107 38-39 0.22399070745141358 1107 40-41 0.23200854671112126 1107 42-43 0.28150544817116696 1107 44-45 0.3129747161729668 1107 46-47 0.28676608478333065 1107 48-49 0.3593152608337675 1107 50-51 0.29321119627520176 1107 52-53 0.2821242771818575 1107 54-55 0.3783632833421464 1107 56-57 0.4235433326093272 1107 58-59 0.3884748300473788 1107 60-61 0.39152920409730996 1107 62-63 0.3785453289751004 1107 64-65 0.32797717489442846 1107 66-67 0.3571110012483345 1107 68-69 0.34298917640208515 1107 70-71 0.3371612110434299 1107 72-73 0.3143339607451381 1107 74-75 0.3246801415558309 1107 76-77 0.3424225348817984 1107 78-79 0.3376440650372885 1107 80-81 0.29396615844376583 1107 82-83 0.3481307190572167 1107 84-85 0.3501395898015147 1107 86-87 0.29774419849618994 1107 88-89 0.26918936576572605 1107 90-91 0.27174945673967343 1107 92-93 0.2758478439176031 1107 94-95 0.31525797568007263 1107 96-97 0.2898276913893021 1107 98-99 0.2750613680347058 1107 100 0.3002854950514475 1108 1 0.1002393182248511 1108 2 0.12875265908536093 1108 3 0.13027596139173525 1108 4 0.12686742454429378 1108 5 0.16900069222423753 1108 6 0.09277193864808453 1108 7 0.13252753887322655 1108 8 0.14942504685573255 1108 9 0.1500216470375335 1108 10-11 0.11174170699565167 1108 12-13 0.11449948397823562 1108 14-15 0.14758467426364774 1108 16-17 0.1249996046247901 1108 18-19 0.11284975645248352 1108 20-21 0.12061805551205396 1108 22-23 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-1.171129390045941 2316 86-87 -1.3563908908294948 2316 88-89 -1.3072183787910632 2316 90-91 -1.1974992531055086 2316 92-93 -1.1336545926927286 2316 94-95 -1.3259755430116549 2316 96-97 -1.4738525966772862 2316 98-99 -1.3190680374616264 2316 100 -1.0878365974833528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17688.0 3 4390.0 4 3119.0 5 2733.0 6 4648.0 7 23394.0 8 45230.0 9 53278.0 10 55679.0 11 53613.0 12 52681.0 13 57096.0 14 65941.0 15 77400.0 16 93095.0 17 112434.0 18 124850.0 19 141036.0 20 161431.0 21 194092.0 22 246881.0 23 346432.0 24 497232.0 25 616107.0 26 625237.0 27 553477.0 28 503667.0 29 495897.0 30 551930.0 31 662807.0 32 820531.0 33 1057921.0 34 1462066.0 35 2249949.0 36 3926727.0 37 7283340.0 38 8565930.0 39 2327307.0 40 4187.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.651965390426717 33.45422001986696 25.635339598842965 25.258474990863355 2 20.77161615046378 31.800037312505765 29.122099073964762 18.30624746306569 3 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21.040400988736536 22-23 24.208185614331104 26.098176702631136 28.63195459039479 21.061683092642962 24-25 21.13350373557225 29.070223434228602 25.407306285261487 24.38896654493766 26-27 21.16372156666299 26.00835579979061 31.727665861819652 21.100256771726748 28-29 21.21238952595871 26.085831817036976 31.632095266075392 21.06968339092892 30-31 21.171269995106 26.02083274662194 31.618321134647477 21.189576123624583 32-33 21.250795650923454 26.113322681944023 31.47869493926283 21.15718672786969 34-35 21.25148091854451 26.225342629266894 31.386997976456716 21.136178475731874 36-37 21.2039151876465 26.187339202121475 31.39223032215415 21.21651528807788 38-39 21.261640719792005 26.205220232579624 31.386560683441278 21.146578364187096 40-41 21.256796228175823 26.237066136926536 31.37283442655664 21.133303208341005 42-43 21.265010179756093 26.255442680320723 31.293548549901324 21.185998590021864 44-45 21.275948129708787 26.307361453169126 31.28867167171122 21.128018745410866 46-47 21.250562352381813 26.435679602880903 31.21362096948913 21.100137075248153 48-49 21.318705045462075 26.41760612825538 31.091231968713767 21.17245685756878 50-51 21.306766777774452 26.48223068248147 31.05101695824392 21.159985581500155 52-53 21.39327004645763 26.646434042061607 30.806361937039604 21.153933974441156 54-55 21.714475271547514 26.874323294576254 30.07798071343453 21.333220720441705 56-57 22.12826758637878 27.372094165423565 29.075154211824554 21.424484036373098 58-59 22.208252650701272 27.86512131910208 28.493889846932912 21.432736183263735 60-61 22.30361393585394 28.07082409263985 28.190554775277292 21.435007196228913 62-63 22.28468073443172 28.200843426688298 28.09973748932595 21.414738349554028 64-65 22.34983813111396 28.27546568186372 28.046018614225453 21.328677572796867 66-67 22.40702044685483 28.099661800289468 28.22219148825791 21.27112626459779 68-69 22.459369452367113 27.93935404822075 28.454032433582082 21.147244065830055 70-71 22.536985345137534 27.856188115715813 28.57803832782661 21.028788211320045 72-73 22.657597632327008 27.78263822887602 28.541432100071596 21.018332038725372 74-75 22.825075398187685 27.848290657970097 28.401790071476622 20.92484387236559 76-77 23.040404007674447 28.016182850766118 28.09668716422295 20.846725977336483 78-79 23.263033938449844 28.153590898873155 27.738330932654492 20.84504423002251 80-81 23.468062802478496 28.308029539712454 27.48033814184018 20.743569515968872 82-83 23.657803671938467 28.444938003215768 27.17099289712271 20.72626542772305 84-85 23.808597451007106 28.489433484736953 26.985547265535857 20.716421798720077 86-87 23.949208407936975 28.514485797261674 26.87901136105567 20.657294433745683 88-89 24.168124245378642 28.57816845206952 26.67681483343083 20.57689246912101 90-91 24.345302633179873 28.54947400816211 26.53676516398314 20.568458194674882 92-93 24.49693077944045 28.551878369670142 26.498252157028134 20.45293869386127 94-95 24.702889262952677 28.560695574459356 26.402568364087426 20.333846798500545 96-97 24.847012533436782 28.52333530589245 26.304572170111484 20.325079990559285 98-99 25.017349225454062 28.500146390848624 26.24934182038072 20.23316256331659 100 25.132416590011374 28.499146879983034 26.113360917508118 20.255075612497475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 65480.0 1 44110.0 2 18904.5 3 13997.5 4 12829.5 5 12480.0 6 12631.0 7 12873.0 8 12759.5 9 12979.5 10 13894.5 11 17847.5 12 140256.5 13 172830.5 14 84593.5 15 88259.0 16 99028.0 17 109524.5 18 119605.0 19 130028.5 20 141520.0 21 157795.0 22 181660.0 23 209742.5 24 237070.0 25 266887.0 26 299468.5 27 340167.5 28 393985.0 29 447642.0 30 492005.5 31 541849.5 32 595871.0 33 634799.5 34 673633.0 35 728284.5 36 785775.5 37 825004.5 38 864922.5 39 910557.0 40 942736.5 41 983577.0 42 1038577.5 43 1060153.0 44 1061895.0 45 1082140.0 46 1102557.5 47 1118987.0 48 1132990.5 49 1143285.0 50 1149523.5 51 1153873.5 52 1149628.5 53 1127762.5 54 1088759.0 55 1033277.0 56 966988.5 57 887487.0 58 792850.5 59 684392.5 60 566889.0 61 458307.0 62 362915.5 63 276042.5 64 205769.0 65 152465.5 66 111577.5 67 83713.0 68 63834.5 69 49792.5 70 39952.5 71 33001.0 72 28407.0 73 23513.0 74 19832.5 75 18049.0 76 14367.5 77 9005.5 78 5735.0 79 4421.0 80 3523.0 81 2849.5 82 2398.5 83 1986.5 84 1615.0 85 1395.0 86 1219.5 87 1047.0 88 927.5 89 819.0 90 684.5 91 594.5 92 518.5 93 434.0 94 393.0 95 329.5 96 263.5 97 219.5 98 171.0 99 121.0 100 72.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4049622609793438 2 0.3848928163660756 3 0.16895297338399745 4 0.1758009537555417 5 0.2741652500846991 6 0.10295695382384575 7 0.1376157013586973 8 0.14324815056933868 9 0.13591688672418248 10-11 0.1326276301128719 12-13 0.13464570473904552 14-15 0.14068381916844605 16-17 0.1447053234670475 18-19 0.15648425976480848 20-21 0.13716317228795155 22-23 0.09971309656914717 24-25 0.09476749568918463 26-27 0.08908671813118205 28-29 0.09943777143872581 30-31 0.10365258912677208 32-33 0.10465283946761142 34-35 0.10524156660819328 36-37 0.10129035808757172 38-39 0.10044827324718723 40-41 0.10153932230125064 42-43 0.06377877356303494 44-45 0.06680734999766999 46-47 0.08543133767622603 48-49 0.08963590389665022 50-51 0.09924152905853185 52-53 0.10440533974930709 54-55 0.10666652060766131 56-57 0.10713662362290205 58-59 0.10654057400544728 60-61 0.10468945185197595 62-63 0.10874024605806906 64-65 0.11560726486948285 66-67 0.12410133804205696 68-69 0.12928565166807635 70-71 0.12767324226066185 72-73 0.11454843471366025 74-75 0.11231361477204851 76-77 0.11403293234180747 78-79 0.11305611392696145 80-81 0.10735483343371473 82-83 0.11032922353949025 84-85 0.11619599201006471 86-87 0.1297938315630562 88-89 0.13752636714084782 90-91 0.14436702503551915 92-93 0.14352786918588378 94-95 0.1337494335698015 96-97 0.13022439320318324 98-99 0.12497564178068227 100 0.11933001211166966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.4141453E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.28562182832133 #Duplication Level Percentage of deduplicated Percentage of total 1 65.71020144712239 26.471763257615855 2 17.33899510089048 13.970243990351802 3 6.7823773769068785 8.196968703090974 4 3.181005925740717 5.125952070321589 5 1.8024013207281726 3.630542899486104 6 1.0833710238320198 2.6186565219514826 7 0.7669968079624684 2.162926034437392 8 0.5303908680032291 1.7093700743658549 9 0.3904021368768007 1.41548335624686 >10 2.1440856259314476 15.664877346711336 >50 0.1575940347037372 4.366147700623577 >100 0.11043646885676975 8.317544578806796 >500 0.001150630018490913 0.29516431091655654 >1k 5.25873917533332E-4 0.47736702902827843 >5k 3.631028293141012E-5 0.08832572230446667 >10k+ 2.9048225973845525E-5 5.488666403741034 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1755687 5.142390981426596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58696 0.17192004101290007 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 41796 0.12242009735203713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.5985154761866753E-4 1.171596299665395E-5 2.9289907491634876E-6 5.857981498326975E-6 5.857981498326975E-6 2 4.6278053836783104E-4 3.221889824079836E-5 2.9289907491634876E-6 8.786972247490463E-6 5.857981498326975E-6 3 4.7742549211364846E-4 4.100587048828882E-5 2.9289907491634876E-6 1.4644953745817437E-5 1.171596299665395E-5 4 4.862124643611389E-4 5.5650824234106264E-5 2.9289907491634876E-6 2.050293524414441E-5 1.4644953745817437E-5 5 4.891414551103024E-4 7.02957779799237E-5 2.9289907491634876E-6 2.050293524414441E-5 1.7573944494980926E-5 6 4.920704458594659E-4 7.615375947825068E-5 2.9289907491634876E-6 2.050293524414441E-5 2.050293524414441E-5 7 4.920704458594659E-4 8.494073172574114E-5 2.9289907491634876E-6 2.050293524414441E-5 2.9289907491634875E-5 8 4.949994366086294E-4 9.079871322406811E-5 2.9289907491634876E-6 2.34319259933079E-5 3.221889824079836E-5 9 5.037864088561198E-4 9.079871322406811E-5 2.9289907491634876E-6 2.34319259933079E-5 3.221889824079836E-5 10-11 5.125733811036104E-4 1.0251467622072207E-4 2.9289907491634876E-6 2.4896421367889643E-5 3.221889824079836E-5 12-13 5.31611820973173E-4 1.2155311609028473E-4 2.9289907491634876E-6 2.636091674247139E-5 3.221889824079836E-5 14-15 5.491857654681539E-4 1.376625652106839E-4 2.9289907491634876E-6 2.636091674247139E-5 3.514788898996185E-5 16-17 5.696887007122983E-4 1.742749495752275E-4 2.9289907491634876E-6 2.636091674247139E-5 3.514788898996185E-5 18-19 5.931206267056062E-4 1.801329310735545E-4 2.9289907491634876E-6 2.9289907491634875E-5 3.661238436454359E-5 20-21 6.28268515695568E-4 2.079583431906076E-4 2.9289907491634876E-6 3.221889824079836E-5 4.100587048828882E-5 22-23 6.85383835304256E-4 2.372482506822425E-4 5.857981498326975E-6 3.9541375113707084E-5 4.68638519866158E-5 24-25 8.040079606453773E-4 2.5482219517722343E-4 5.857981498326975E-6 4.100587048828882E-5 5.7115319608688003E-5 26-27 0.001246285563769064 2.738606350467861E-4 5.857981498326975E-6 4.3934861237452316E-5 6.736678723076021E-5 28-29 0.002731283873594952 2.899700841671853E-4 5.857981498326975E-6 4.9792842735779286E-5 6.736678723076021E-5 30-31 0.006736678723076022 3.0022155178925744E-4 5.857981498326975E-6 6.590229185617847E-5 6.883128260534195E-5 32-33 0.015416742808222018 3.192599916588201E-4 7.322476872908719E-6 7.176027335450544E-5 7.761825485283242E-5 34-35 0.03168728641982519 3.456209084012915E-4 8.786972247490463E-6 7.322476872908718E-5 8.054724560199591E-5 36-37 0.05849194526079485 3.6026586214710893E-4 8.786972247490463E-6 7.468926410366894E-5 8.786972247490463E-5 38-39 0.09776092423482974 3.7198182514376296E-4 8.786972247490463E-6 7.615375947825068E-5 9.37277039732316E-5 40-41 0.15264728188340432 3.880912742641621E-4 8.786972247490463E-6 7.615375947825068E-5 9.37277039732316E-5 42-43 0.2249977468738662 3.983427418862343E-4 8.786972247490463E-6 7.615375947825068E-5 1.0837265771904904E-4 44-45 0.31778231582586713 4.159166863812152E-4 8.786972247490463E-6 7.615375947825068E-5 1.1130164846821253E-4 46-47 0.43427852938772116 4.3056164012703264E-4 8.786972247490463E-6 8.054724560199591E-5 1.1276614384279427E-4 48-49 0.5761544477910767 4.4227760312368666E-4 8.786972247490463E-6 8.201174097657765E-5 1.1423063921737602E-4 50-51 0.7429970833403019 4.525290707457588E-4 8.786972247490463E-6 8.201174097657765E-5 1.1423063921737602E-4 52-53 0.9346424125534434 4.68638519866158E-4 8.786972247490463E-6 8.201174097657765E-5 1.171596299665395E-4 54-55 1.1541439082865046 4.818189782373937E-4 8.786972247490463E-6 8.34762363511594E-5 1.171596299665395E-4 56-57 1.4061308404185375 5.330763163477548E-4 8.786972247490463E-6 8.494073172574114E-5 1.171596299665395E-4 58-59 1.6934648329114756 5.623662238393896E-4 8.786972247490463E-6 8.640522710032288E-5 1.2008862071570299E-4 60-61 2.0138685954578444 5.828691590835341E-4 8.786972247490463E-6 8.786972247490463E-5 1.3180458371235695E-4 62-63 2.3643076936415097 6.311975064447315E-4 8.786972247490463E-6 1.0251467622072207E-4 1.3619806983610215E-4 64-65 2.744739657096609 6.648809000601117E-4 8.786972247490463E-6 1.1423063921737602E-4 1.376625652106839E-4 66-67 3.157954349511721 6.795258538059291E-4 8.786972247490463E-6 1.1862412534112125E-4 1.435205467090109E-4 68-69 3.6053620799325676 6.839193399296743E-4 8.786972247490463E-6 1.2301761146486648E-4 1.4791403283275612E-4 70-71 4.0870858659706135 7.014932844246554E-4 8.786972247490463E-6 1.2301761146486648E-4 1.5230751895650135E-4 72-73 4.597326305942515 7.205317242942179E-4 8.786972247490463E-6 1.2301761146486648E-4 1.5816550045482834E-4 74-75 5.136925484688657 7.48357136411271E-4 8.786972247490463E-6 1.2594660221402996E-4 1.5816550045482834E-4 76-77 5.707412335380102 7.747180531537425E-4 8.786972247490463E-6 1.2887559296319344E-4 1.6109449120399181E-4 78-79 6.30812783509829 7.835050254012329E-4 8.786972247490463E-6 1.303400883377752E-4 1.640234819531553E-4 80-81 6.938449573309022 8.215819051403582E-4 8.786972247490463E-6 1.3180458371235695E-4 1.669524727023188E-4 82-83 7.593541786285428 8.376913542607574E-4 8.786972247490463E-6 1.332690790869387E-4 1.71345958826064E-4 84-85 8.272360288825436 8.772327293744645E-4 8.786972247490463E-6 1.3912706058526566E-4 1.7866843569897274E-4 86-87 8.975143793675096 9.006646553677724E-4 8.786972247490463E-6 1.435205467090109E-4 1.7866843569897274E-4 88 9.517597273906299 9.138451137390082E-4 8.786972247490463E-6 1.435205467090109E-4 1.7866843569897274E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 235620 0.0 84.468506 8 GTGGTAT 237365 0.0 83.96137 6 AGTGGTA 237545 0.0 83.914986 5 GTATCAA 238110 0.0 83.50071 9 TGGTATC 237660 0.0 83.4719 7 AAGCAGT 244940 0.0 81.63517 1 GCAGTGG 249245 0.0 80.35394 3 CAGTGGT 249945 0.0 79.63154 4 AGCAGTG 259360 0.0 77.253815 2 CAACGCA 231815 0.0 44.297226 12-13 ATCAACG 233925 0.0 44.01236 10-11 ACGCAGA 235115 0.0 43.760143 14-15 AGAGTAC 233275 0.0 43.21603 18-19 TCAACGC 231165 0.0 43.080658 12-13 AACGCAG 234145 0.0 42.656387 14-15 GAGTACT 225330 0.0 42.54444 20-21 AGTACTT 234580 0.0 42.08308 20-21 CGCAGAG 237835 0.0 41.90308 16-17 TATCAAC 238190 0.0 41.67918 10-11 GTACTTT 233695 0.0 40.762905 22-23 >>END_MODULE