FastQCFastQC Report
Thu 26 May 2016
SRR1660746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34141453
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17496765.124784818033374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA474080.13885759343634263No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT447200.13098446630259117No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385470.11290380640800496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450900.084.063578
GTGGTAT2440800.083.750436
GTATCAA2469950.083.2954949
AGTGGTA2455100.083.281745
TGGTATC2475150.083.24497
AAGCAGT2411000.082.644971
GCAGTGG2496800.081.916943
CAGTGGT2573700.079.291464
AGCAGTG2605400.078.169972
CAACGCA2390250.044.31389212-13
ATCAACG2415800.043.95499410-11
ACGCAGA2430800.043.68774814-15
AGAGTAC2445350.043.2334518-19
TCAACGC2385000.042.97576512-13
AACGCAG2410100.042.6179114-15
GAGTACT2367950.042.47703620-21
AGTACTT2468200.042.10774620-21
CGCAGAG2461200.041.7484716-17
TATCAAC2475350.041.4991210-11
GTACTTT2452200.040.79252622-23
Produced by FastQC (version 0.11.5)