FastQCFastQC Report
Thu 26 May 2016
SRR1660745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27770456
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14622215.2653834708367775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA593660.21377394739214942No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT356760.12846746196749523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1854400.085.375918
GTGGTAT1882600.084.123236
AGTGGTA1883450.084.086985
TGGTATC1879600.083.944147
GTATCAA1890450.083.651159
AAGCAGT1922600.082.8427351
GCAGTGG1947550.081.817263
CAGTGGT1970450.080.2764744
AGCAGTG2009350.079.277592
CAACGCA1828850.044.74436612-13
ATCAACG1840150.044.60083810-11
ACGCAGA1848650.044.2541914-15
AGAGTAC1845200.043.59138518-19
TCAACGC1824800.043.4848112-13
AACGCAG1844000.043.1037614-15
GAGTACT1810950.042.7935420-21
CGCAGAG1863800.042.52806516-17
AGTACTT1898300.042.08232520-21
TATCAAC1885750.041.8885110-11
GTACTTT1905250.040.6883122-23
Produced by FastQC (version 0.11.5)