FastQCFastQC Report
Thu 26 May 2016
SRR1660745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27770456
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14789475.325612946362853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535040.19266518345971703No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT378960.13646156908622603No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT328350.11823716542501139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1952650.085.4961558
AAGCAGT1912600.084.398321
TGGTATC1983050.084.129577
GTGGTAT1972650.084.0329366
GTATCAA1988300.083.868649
AGTGGTA1979050.083.803895
GCAGTGG1983450.083.6245353
CAGTGGT2050800.080.7305454
AGCAGTG2051550.080.631962
CAACGCA1918700.044.73004512-13
ATCAACG1931800.044.50325410-11
ACGCAGA1946250.044.22493414-15
AGAGTAC1972800.043.49809318-19
TCAACGC1915850.043.40863812-13
AACGCAG1937300.043.04696314-15
GAGTACT1932750.042.74464420-21
CGCAGAG1960450.042.5364816-17
AGTACTT2024200.042.13767220-21
TATCAAC1985900.041.9247310-11
GTACTTT2020500.040.85887522-23
Produced by FastQC (version 0.11.5)