FastQCFastQC Report
Thu 26 May 2016
SRR1660744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36439376
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16637624.565835594989332No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT465280.12768605038681233No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400250.10983997091497943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2144100.084.430268
AGTGGTA2164950.083.733245
GTGGTAT2177600.083.1900256
TGGTATC2176800.082.927847
GTATCAA2181900.082.867089
AAGCAGT2260550.080.7337951
GCAGTGG2287600.079.820733
CAGTGGT2316100.078.262824
AGCAGTG2379100.076.8078162
CAACGCA2107850.044.6436612-13
ATCAACG2125250.044.33382810-11
ACGCAGA2141750.043.96817414-15
AGAGTAC2140250.043.30340218-19
TCAACGC2104100.043.14711412-13
AACGCAG2130700.042.70026414-15
GAGTACT2091400.042.47403720-21
CGCAGAG2159900.042.04058516-17
AGTACTT2218050.041.50675620-21
TATCAAC2184200.041.35124210-11
CAGAGTA2258650.039.72210318-19
Produced by FastQC (version 0.11.5)