##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660744_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36439376 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798389796795643 34.0 31.0 34.0 30.0 34.0 2 31.917721121239836 34.0 31.0 34.0 30.0 34.0 3 31.976166551260373 34.0 31.0 34.0 30.0 34.0 4 35.377138071738656 37.0 35.0 37.0 33.0 37.0 5 35.34350214998193 37.0 35.0 37.0 33.0 37.0 6 35.41674997398419 37.0 35.0 37.0 33.0 37.0 7 35.38233127263211 37.0 35.0 37.0 33.0 37.0 8 35.3822704867394 37.0 35.0 37.0 33.0 37.0 9 37.056127031373975 39.0 37.0 39.0 33.0 39.0 10-11 37.00695477057565 39.0 37.0 39.0 33.0 39.0 12-13 37.012909963112435 39.0 37.0 39.0 33.0 39.0 14-15 38.37503706155671 40.0 38.0 41.0 33.0 41.0 16-17 38.354202374376555 40.0 38.0 41.0 33.0 41.0 18-19 38.32163845231598 40.0 38.0 41.0 33.5 41.0 20-21 38.210542504899095 40.0 38.0 41.0 33.0 41.0 22-23 38.05154804242531 40.0 38.0 41.0 33.0 41.0 24-25 38.024100110825174 40.0 38.0 41.0 33.0 41.0 26-27 37.99128680194743 40.0 38.0 41.0 33.0 41.0 28-29 37.90238566379402 40.0 38.0 41.0 32.0 41.0 30-31 37.80867363096448 40.0 38.0 41.0 32.0 41.0 32-33 37.68139867433515 40.0 38.0 41.0 32.0 41.0 34-35 37.523383742904926 40.0 37.5 41.0 31.0 41.0 36-37 37.34133225826919 40.0 37.0 41.0 31.0 41.0 38-39 36.96483611574468 40.0 35.5 41.0 30.5 41.0 40-41 36.90320020847777 39.5 35.0 41.0 30.0 41.0 42-43 36.91767663639465 40.0 35.0 41.0 30.0 41.0 44-45 36.921389282297255 40.0 35.0 41.0 30.0 41.0 46-47 37.01997248800309 40.0 35.5 41.0 31.0 41.0 48-49 36.91788890676942 40.0 35.0 41.0 30.0 41.0 50-51 35.56230744730645 38.5 34.0 40.0 28.0 40.5 52-53 35.743339197136635 38.5 35.0 39.5 28.5 40.5 54-55 36.202536604907834 39.0 35.0 41.0 27.5 41.0 56-57 35.901642250954026 39.0 35.0 41.0 26.0 41.0 58-59 35.72944304809171 39.0 35.0 41.0 26.0 41.0 60-61 35.50402493171123 39.0 35.0 41.0 26.0 41.0 62-63 35.27203816552731 38.5 35.0 41.0 25.5 41.0 64-65 35.0105925935724 38.0 34.5 40.5 25.0 41.0 66-67 34.72874292633331 37.0 34.0 40.0 25.0 41.0 68-69 34.43649472757163 37.0 34.0 40.0 25.0 41.0 70-71 34.1210191826556 36.0 34.0 39.0 24.0 41.0 72-73 33.77103309892024 36.0 33.5 39.0 24.0 41.0 74-75 33.38943461874868 35.0 33.0 38.5 23.5 40.0 76-77 32.993220671506556 35.0 33.0 37.0 23.0 39.5 78-79 32.62072266275909 35.0 33.0 37.0 21.5 39.0 80-81 32.30057153009426 35.0 33.0 36.0 20.5 39.0 82-83 31.990668377526553 35.0 32.0 36.0 20.0 37.5 84-85 31.706142278616408 35.0 32.0 35.5 20.0 37.0 86-87 31.49051771632972 35.0 32.0 35.0 19.5 37.0 88-89 31.28289869727736 35.0 32.0 35.0 19.0 36.0 90-91 31.11011815899372 35.0 31.5 35.0 18.5 36.0 92-93 30.957627594940156 35.0 31.0 35.0 18.0 36.0 94-95 30.77774720950216 35.0 31.0 35.0 18.0 35.5 96-97 30.638095174846022 35.0 31.0 35.0 17.0 35.0 98-99 30.53105939574816 34.5 31.0 35.0 17.0 35.0 100 30.448528289836798 34.0 31.0 35.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5028527955195905 1101 2 0.5311878252440536 1101 3 0.5317847512273346 1101 4 0.4471908145909751 1101 5 0.47066895907843787 1101 6 0.4452071749532891 1101 7 0.47094176152287304 1101 8 0.4765966929184131 1101 9 0.5645858431207671 1101 10-11 0.5589293578537067 1101 12-13 0.5485570047052946 1101 14-15 0.6670103640337004 1101 16-17 0.6322685078344961 1101 18-19 0.6304624937771948 1101 20-21 0.6664563733094937 1101 22-23 0.7108739239964947 1101 24-25 0.7176486105157878 1101 26-27 0.6873147068795475 1101 28-29 0.6835100577141944 1101 30-31 0.6532641343810326 1101 32-33 0.6474190129567887 1101 34-35 0.6689296785799286 1101 36-37 0.6406641200194656 1101 38-39 0.6516306394900298 1101 40-41 0.651499028482192 1101 42-43 0.6691082883256954 1101 44-45 0.6718372467250049 1101 46-47 0.6995327278886023 1101 48-49 0.7569035973876836 1101 50-51 0.6700222671407374 1101 52-53 0.6876269027277928 1101 54-55 0.7304855796818472 1101 56-57 0.7516967038845834 1101 58-59 0.6556682779619081 1101 60-61 0.663731646497574 1101 62-63 0.608448172686515 1101 64-65 0.5707937509186038 1101 66-67 0.5759934765754764 1101 68-69 0.5596757771064844 1101 70-71 0.5226895107724445 1101 72-73 0.5105673637599537 1101 74-75 0.5129109425949778 1101 76-77 0.48255853465882836 1101 78-79 0.5001380524428711 1101 80-81 0.4129546521223304 1101 82-83 0.41577741136383395 1101 84-85 0.4166468399591672 1101 86-87 0.3783828148762858 1101 88-89 0.36403859459879584 1101 90-91 0.37531479938249745 1101 92-93 0.380866282770878 1101 94-95 0.41663957476992053 1101 96-97 0.40221036236408736 1101 98-99 0.3686346236693794 1101 100 0.381622231575264 1102 1 0.44833804622859574 1102 2 0.5059297460981611 1102 3 0.4937878051124116 1102 4 0.4227861208918 1102 5 0.44622132164391104 1102 6 0.40023461178596165 1102 7 0.41271564877292377 1102 8 0.44035136931353236 1102 9 0.5057536944645094 1102 10-11 0.49251612344669127 1102 12-13 0.501027430623715 1102 14-15 0.5864987824068848 1102 16-17 0.5651651430838385 1102 18-19 0.5358972384157639 1102 20-21 0.5694769449947259 1102 22-23 0.6108204794139098 1102 24-25 0.5739710412123955 1102 26-27 0.5536570698258387 1102 28-29 0.5558794761794204 1102 30-31 0.5445734464485525 1102 32-33 0.5513699087993942 1102 34-35 0.5314142001314011 1102 36-37 0.5663501102138753 1102 38-39 0.580469283942918 1102 40-41 0.5583090109707527 1102 42-43 0.5787619233101537 1102 44-45 0.5575938203697532 1102 46-47 0.6068660370523062 1102 48-49 0.6414037374646142 1102 50-51 0.6106507248239552 1102 52-53 0.6420693090987797 1102 54-55 0.6755366045834705 1102 56-57 0.6881055282263944 1102 58-59 0.5955577949484336 1102 60-61 0.6213411474912718 1102 62-63 0.5988303463364844 1102 64-65 0.57089080441677 1102 66-67 0.57466154809903 1102 68-69 0.5349319352456376 1102 70-71 0.5338591547745466 1102 72-73 0.48670236841977754 1102 74-75 0.46994253135230224 1102 76-77 0.4438022939885826 1102 78-79 0.44566914152281356 1102 80-81 0.4363001881142452 1102 82-83 0.43322726141560963 1102 84-85 0.41418330585166174 1102 86-87 0.3569759065722842 1102 88-89 0.36659699540690127 1102 90-91 0.35298651568117023 1102 92-93 0.36038809075379774 1102 94-95 0.40580747340287004 1102 96-97 0.353512344604912 1102 98-99 0.3193195633571726 1102 100 0.2800889156838089 1103 1 0.3650369752176097 1103 2 0.3842492857462716 1103 3 0.39856277899160375 1103 4 0.32255881633552264 1103 5 0.36545441642157783 1103 6 0.32339485810266666 1103 7 0.31234948448202715 1103 8 0.360464388941665 1103 9 0.37904093415090045 1103 10-11 0.36787910938468826 1103 12-13 0.40288150965645286 1103 14-15 0.4813686593777078 1103 16-17 0.4530335894627484 1103 18-19 0.4407733828461389 1103 20-21 0.4580739382371135 1103 22-23 0.4910304679720099 1103 24-25 0.5502843885126154 1103 26-27 0.4914161733888065 1103 28-29 0.4885153459829681 1103 30-31 0.4304215077355451 1103 32-33 0.38383505629028747 1103 34-35 0.43327959829068163 1103 36-37 0.4465027908403272 1103 38-39 0.4265110213280181 1103 40-41 0.3976009269243761 1103 42-43 0.45480724719008947 1103 44-45 0.4907329315234108 1103 46-47 0.48924759749326796 1103 48-49 0.5193632260606122 1103 50-51 0.42564638603431604 1103 52-53 0.4038839713071454 1103 54-55 0.4798671592953738 1103 56-57 0.5053569893314815 1103 58-59 0.4281570838628568 1103 60-61 0.4118378160037466 1103 62-63 0.3810763795307821 1103 64-65 0.36116959674746596 1103 66-67 0.3999100907321633 1103 68-69 0.3875733885731947 1103 70-71 0.35840451267131357 1103 72-73 0.3437613209543713 1103 74-75 0.3402407948728623 1103 76-77 0.33607828399168227 1103 78-79 0.3608891001025327 1103 80-81 0.30652954394154364 1103 82-83 0.32928475388946765 1103 84-85 0.3086547570661473 1103 86-87 0.27993851081744836 1103 88-89 0.26593817104241424 1103 90-91 0.24195887654533266 1103 92-93 0.21603137215537416 1103 94-95 0.2824520581380021 1103 96-97 0.2534916281665325 1103 98-99 0.25885641460498476 1103 100 0.3095347544275633 1104 1 0.36015821192360775 1104 2 0.39946994504762756 1104 3 0.3785269293959672 1104 4 0.29879923908375616 1104 5 0.3187580054062451 1104 6 0.27011690104389174 1104 7 0.24567373386893365 1104 8 0.2923354698226177 1104 9 0.3765395036886048 1104 10-11 0.3346822812179795 1104 12-13 0.34203836513164987 1104 14-15 0.42885653893297615 1104 16-17 0.4251536994439462 1104 18-19 0.4081937760818377 1104 20-21 0.3477030483229626 1104 22-23 0.3941682681203602 1104 24-25 0.43410864642787317 1104 26-27 0.36741229185895463 1104 28-29 0.3745596391016548 1104 30-31 0.3409062413133199 1104 32-33 0.3451602614111309 1104 34-35 0.3394058803662432 1104 36-37 0.3436754616261197 1104 38-39 0.30952943390972365 1104 40-41 0.3397705390953192 1104 42-43 0.30499552734460167 1104 44-45 0.3371021105617231 1104 46-47 0.3645445490810246 1104 48-49 0.3974387374480486 1104 50-51 0.3613740967727779 1104 52-53 0.3634435655697459 1104 54-55 0.406129887637654 1104 56-57 0.45153939619560646 1104 58-59 0.41903276491390784 1104 60-61 0.42406112349204506 1104 62-63 0.408426241767593 1104 64-65 0.4035984992650299 1104 66-67 0.41464046123477516 1104 68-69 0.4273950906051738 1104 70-71 0.3844498969006338 1104 72-73 0.35596166895005865 1104 74-75 0.3331721195847308 1104 76-77 0.3403907786159692 1104 78-79 0.339423450335957 1104 80-81 0.31947194539078083 1104 82-83 0.3112412421982498 1104 84-85 0.2769525460479194 1104 86-87 0.2598625807055903 1104 88-89 0.24120547008823223 1104 90-91 0.21019786601175028 1104 92-93 0.2404492220771921 1104 94-95 0.309276301609156 1104 96-97 0.2866152909932218 1104 98-99 0.25690508309526194 1104 100 0.18660045797985347 1105 1 0.2924048678341187 1105 2 0.3315948307900669 1105 3 0.3243621968156418 1105 4 0.2953356952618549 1105 5 0.2981909289854485 1105 6 0.2584118705003249 1105 7 0.3001263073962761 1105 8 0.2836514290865395 1105 9 0.30322108392547875 1105 10-11 0.3154282545124971 1105 12-13 0.3107617071479538 1105 14-15 0.365395777796536 1105 16-17 0.30935158205650737 1105 18-19 0.30034704134086354 1105 20-21 0.32576412919144104 1105 22-23 0.3763657957006359 1105 24-25 0.4100838549701322 1105 26-27 0.3860654854749086 1105 28-29 0.38418241637858586 1105 30-31 0.39237898866439025 1105 32-33 0.37159747554809286 1105 34-35 0.3872718000442461 1105 36-37 0.37004926956232964 1105 38-39 0.376110207306219 1105 40-41 0.36469057610060673 1105 42-43 0.39616968128965624 1105 44-45 0.4385401343162272 1105 46-47 0.4457297410424985 1105 48-49 0.44495944635746554 1105 50-51 0.31886386399135347 1105 52-53 0.4036133186091817 1105 54-55 0.4864961087886357 1105 56-57 0.4940416619714014 1105 58-59 0.4035411600711143 1105 60-61 0.4148757265197247 1105 62-63 0.39753039050619066 1105 64-65 0.4135765556312023 1105 66-67 0.3789568081924699 1105 68-69 0.3582393459328017 1105 70-71 0.37074519407916284 1105 72-73 0.35049392498923737 1105 74-75 0.3526574293397857 1105 76-77 0.37030798265185183 1105 78-79 0.3735195979312138 1105 80-81 0.3027766122661575 1105 82-83 0.2928792341031823 1105 84-85 0.29922875046126407 1105 86-87 0.26324101780882714 1105 88-89 0.28696957650897303 1105 90-91 0.2564307894248863 1105 92-93 0.26237983051930414 1105 94-95 0.30600981085323653 1105 96-97 0.284959087998903 1105 98-99 0.3048722995360649 1105 100 0.27025367558447044 1106 1 0.2366436636288647 1106 2 0.2729650956868994 1106 3 0.26683665375733767 1106 4 0.2635298205082037 1106 5 0.2478951889495633 1106 6 0.21914195170698036 1106 7 0.2598669901183257 1106 8 0.27802624256587904 1106 9 0.2836655786804769 1106 10-11 0.26415796468737085 1106 12-13 0.2523913115938612 1106 14-15 0.3583069177048088 1106 16-17 0.3219459265018969 1106 18-19 0.29231852928586477 1106 20-21 0.30740375385082785 1106 22-23 0.31142535226772594 1106 24-25 0.30639183649523005 1106 26-27 0.3134994376433937 1106 28-29 0.3149877570860724 1106 30-31 0.2830479333341742 1106 32-33 0.3062909985269542 1106 34-35 0.336772897447446 1106 36-37 0.28634024670094504 1106 38-39 0.2918551003456926 1106 40-41 0.2919994375142707 1106 42-43 0.3131068550657048 1106 44-45 0.3171803634702144 1106 46-47 0.32858915639531006 1106 48-49 0.3555769367971422 1106 50-51 0.214047437947535 1106 52-53 0.27932962832606023 1106 54-55 0.42534459905479594 1106 56-57 0.44111230914823096 1106 58-59 0.37384792661686106 1106 60-61 0.37175074159113564 1106 62-63 0.3798956416356347 1106 64-65 0.3342012398996346 1106 66-67 0.33309819137461716 1106 68-69 0.3283667429008261 1106 70-71 0.31223595599169585 1106 72-73 0.2845252186819067 1106 74-75 0.29877450638133496 1106 76-77 0.30606890680414267 1106 78-79 0.31674385831105667 1106 80-81 0.2615356965164821 1106 82-83 0.2939234438697156 1106 84-85 0.2948791002791644 1106 86-87 0.21845705947865923 1106 88-89 0.24573375111888396 1106 90-91 0.2708914155895421 1106 92-93 0.24377203964013106 1106 94-95 0.27919249104620647 1106 96-97 0.32575526861903725 1106 98-99 0.2811157372984354 1106 100 0.25027636038730705 1107 1 0.21703856301982327 1107 2 0.23814965968678337 1107 3 0.23305920315472406 1107 4 0.2437277028493341 1107 5 0.23845847149702593 1107 6 0.2111259861513659 1107 7 0.2126205415068867 1107 8 0.22146153102306698 1107 9 0.24909299226329296 1107 10-11 0.22704008549808918 1107 12-13 0.22933493228806157 1107 14-15 0.27355753228924584 1107 16-17 0.2427159415611584 1107 18-19 0.2538073550118014 1107 20-21 0.2581253992871595 1107 22-23 0.2710204008182515 1107 24-25 0.2962388328344261 1107 26-27 0.27529129228809524 1107 28-29 0.2907714178179859 1107 30-31 0.24438033321375485 1107 32-33 0.1530746120145423 1107 34-35 0.26404949822320134 1107 36-37 0.23525061045403106 1107 38-39 0.2554542006768159 1107 40-41 0.2366446460925573 1107 42-43 0.30167250125776235 1107 44-45 0.313687622112397 1107 46-47 0.2911207314343258 1107 48-49 0.343827745741784 1107 50-51 0.2740433500565018 1107 52-53 0.27208295766597956 1107 54-55 0.34935843557351376 1107 56-57 0.379192331207868 1107 58-59 0.3177893372569258 1107 60-61 0.33789427621426427 1107 62-63 0.30905945062148277 1107 64-65 0.2762120995067292 1107 66-67 0.30003916944454545 1107 68-69 0.29071106349562825 1107 70-71 0.3047264783479875 1107 72-73 0.2826958723440143 1107 74-75 0.2841880606742748 1107 76-77 0.28670485780692445 1107 78-79 0.3127290108386447 1107 80-81 0.260056314323144 1107 82-83 0.26676677059863607 1107 84-85 0.2518956443221576 1107 86-87 0.2463254862775628 1107 88-89 0.2701196730403055 1107 90-91 0.27379746308567476 1107 92-93 0.21852792864597248 1107 94-95 0.25645380482033175 1107 96-97 0.29284345380416354 1107 98-99 0.3031742422433936 1107 100 0.3016275668245072 1108 1 0.14317595507166914 1108 2 0.16319791105137682 1108 3 0.19220838321149358 1108 4 0.16735073170900705 1108 5 0.15999890014187912 1108 6 0.11298295535341651 1108 7 0.14461377926302532 1108 8 0.1432343917412453 1108 9 0.17799841654196769 1108 10-11 0.1347342144949124 1108 12-13 0.14457937811477706 1108 14-15 0.17122553775953264 1108 16-17 0.16100300145994595 1108 18-19 0.13319827483253022 1108 20-21 0.11715027233025666 1108 22-23 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-1.1927111047148387 2316 82-83 -1.1324087009968622 2316 84-85 -1.228498664686711 2316 86-87 -1.4652652163390485 2316 88-89 -1.3969671278052118 2316 90-91 -1.3100923878664155 2316 92-93 -1.1872023736205186 2316 94-95 -1.4325640055675812 2316 96-97 -1.5881179511150698 2316 98-99 -1.4054691888301107 2316 100 -1.205425285245859 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18815.0 3 4832.0 4 3377.0 5 2757.0 6 5318.0 7 29558.0 8 57963.0 9 67393.0 10 68554.0 11 63241.0 12 58116.0 13 58326.0 14 63636.0 15 74356.0 16 91352.0 17 112220.0 18 124329.0 19 137839.0 20 157176.0 21 189877.0 22 241777.0 23 344709.0 24 492918.0 25 599686.0 26 599724.0 27 531891.0 28 499499.0 29 506459.0 30 568883.0 31 690245.0 32 860498.0 33 1115712.0 34 1532603.0 35 2321665.0 36 4041057.0 37 7668645.0 38 9570278.0 39 2858598.0 40 5494.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.496639947713845 32.765286247137496 26.93186680807294 24.806206997075712 2 20.633311050145327 32.01527440635819 29.131252642260286 18.2201619012362 3 24.234961679670285 26.02252809799695 27.229514781055965 22.5129954412768 4 18.75379546482331 28.3508418406269 25.690328557702745 27.20503413684704 5 21.81956529342585 31.90467779017421 26.66533055643286 19.610426359967086 6 26.98572441672654 27.57774678713185 26.34969581748527 19.086832978656336 7 20.99122013859601 26.61129491324469 31.812823690937314 20.584661257221985 8 25.52168166260089 26.70420013034845 26.910473867307587 20.86364433974307 9 25.10579742810532 25.180815648857457 28.591810249381243 21.121576673655984 10-11 20.693657010077207 29.38680098178013 29.19995141661244 20.719590591530224 12-13 20.680112794886945 26.892968626641238 28.945905697622404 23.48101288084941 14-15 20.64817280269645 32.19298523081663 26.360358609461247 20.79848335702567 16-17 23.398616295218606 26.725508906180217 26.405601327406348 23.47027347119483 18-19 20.687008459067403 29.413880320353865 26.42849955265142 23.47061166792731 20-21 23.36528257362944 29.52503738571568 26.38319041216382 20.726489628491066 22-23 23.363222946784227 26.873911056652595 29.00531648153228 20.757549515030902 24-25 20.7860201517026 29.37392272712583 26.231849003187513 23.608208117984056 26-27 20.803948950166216 26.76541660645002 31.655844929480708 20.77478951390305 28-29 20.786031634930143 26.85828122126362 31.636640161151043 20.719046982655193 30-31 20.74604032296757 26.78850220683224 31.62157257790634 20.843884892293847 32-33 20.868472527241284 26.92139481389425 31.422128663610028 20.78800399525444 34-35 20.87204026554345 27.041512279515796 31.303599675157706 20.782847779783047 36-37 20.795288541557298 26.997374574912275 31.37367596268013 20.833660920850296 38-39 20.856263253629223 26.986829159817944 31.400415600298494 20.756491986254343 40-41 20.87670843771058 27.034723799880283 31.360225875154967 20.728341887254167 42-43 20.879719033669673 27.05986450584572 31.315679169473583 20.744737291011024 44-45 20.902168384522493 27.136449467190243 31.29517610782646 20.666206040460803 46-47 20.89882095636164 27.236354924029104 31.217258706237 20.647565413372256 48-49 20.981244945204157 27.25456237878155 31.09052487776371 20.673667798250587 50-51 21.00537455712952 27.3148378654961 31.04834774703053 20.631439830343854 52-53 21.12593965146318 27.432205505479345 30.796919217188044 20.64493562586943 54-55 21.441627642889987 27.649701696247046 30.14027296197563 20.768397698887334 56-57 21.879684666127908 28.113746027011643 29.19593236211437 20.81063694474608 58-59 22.02445941022689 28.5449688365641 28.64092606846787 20.78964568474114 60-61 22.201599819699005 28.698512351958794 28.35431771874847 20.745570109593732 62-63 22.266970223584007 28.837195604306604 28.20288928525593 20.692944886853454 64-65 22.390761338746696 28.895496871924685 28.12374290713372 20.589998882194894 66-67 22.506071329771792 28.732951449914662 28.257657497647138 20.503319722666404 68-69 22.64737676007888 28.586479833719753 28.413733109698093 20.352410296503272 70-71 22.79504888756677 28.497834914612625 28.472792867136793 20.234323330683814 72-73 22.97220137032509 28.417524055694106 28.45004271233236 20.160231861648448 74-75 23.22389843193626 28.492114445906182 28.27589118158025 20.008095940577313 76-77 23.522559378898716 28.589801149929407 27.963783909519037 19.923855561652843 78-79 23.813676743639924 28.705104552080424 27.607226144610397 19.873992559669254 80-81 24.09117741373521 28.850616213125956 27.306875668305686 19.751330704833148 82-83 24.329236152446757 28.98321676200674 26.970610651769185 19.716936433777317 84-85 24.5142321023495 28.991945822365857 26.80553832276122 19.68828375252342 86-87 24.723735773373313 28.96760582251492 26.703607872637402 19.60505053147436 88-89 24.978193399136902 28.957173597416002 26.53678174006026 19.52785126338683 90-91 25.173613002920913 28.90356578311925 26.436991929547975 19.48582928441186 92-93 25.362604855610627 28.890545735474486 26.37300110607999 19.3738483028349 94-95 25.563212495645516 28.858202108502507 26.27776945299235 19.300815942859625 96-97 25.71895410558466 28.775150166148432 26.197818099801832 19.30807762846507 98-99 25.856895989529622 28.76608681098776 26.15065416109943 19.226363038383198 100 25.950336981430866 28.768392176520273 26.041833867855097 19.23943697419376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46388.0 1 33496.5 2 17573.0 3 13596.5 4 12451.5 5 12346.0 6 12668.0 7 12977.0 8 13056.5 9 13223.5 10 14115.0 11 17823.5 12 133235.0 13 164062.5 14 80734.0 15 84254.5 16 95081.5 17 105638.5 18 115241.0 19 125054.0 20 136733.0 21 155667.0 22 184101.0 23 216113.5 24 249510.0 25 287467.5 26 332685.0 27 387572.5 28 454887.5 29 522756.5 30 580542.5 31 637620.5 32 699658.5 33 754519.5 34 799707.0 35 855858.0 36 919478.0 37 977278.0 38 1036494.5 39 1079676.0 40 1101519.5 41 1134714.0 42 1178224.5 43 1193268.5 44 1197439.0 45 1218347.0 46 1240642.5 47 1257536.0 48 1263671.5 49 1259070.0 50 1246281.0 51 1222824.5 52 1186217.5 53 1130792.5 54 1056515.0 55 978646.0 56 901251.5 57 813991.5 58 715973.5 59 612958.0 60 505284.0 61 404366.5 62 315157.0 63 235503.5 64 172606.0 65 125787.5 66 91452.0 67 67240.5 68 49664.5 69 37377.0 70 29056.5 71 23013.0 72 18878.0 73 15423.0 74 12834.5 75 11188.5 76 9132.0 77 6639.5 78 4899.5 79 3968.0 80 3299.0 81 2796.5 82 2397.0 83 2002.5 84 1735.5 85 1527.5 86 1318.0 87 1130.0 88 942.5 89 806.5 90 705.5 91 612.0 92 537.5 93 461.5 94 387.0 95 334.0 96 274.0 97 210.5 98 170.5 99 130.0 100 75.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.402822485214895 2 0.38409549054846603 3 0.16857313912290922 4 0.17488224825803822 5 0.27459032229311503 6 0.10348969751842073 7 0.13805121141481677 8 0.1435013596281122 9 0.135979276922854 10-11 0.1326477160311417 12-13 0.1346729976934841 14-15 0.14099857253318498 16-17 0.14525358502297075 18-19 0.15706361162715848 20-21 0.1376080095334234 22-23 0.10027888512690228 24-25 0.09511825888566258 26-27 0.0895638278767452 28-29 0.09951048558021411 30-31 0.10363651671752008 32-33 0.10480420960007658 34-35 0.10518154866318238 36-37 0.10123664027616719 38-39 0.10047098501357432 40-41 0.10144520586741113 42-43 0.0640515907846501 44-45 0.06711283969297388 46-47 0.08568752659211289 48-49 0.089841000570372 50-51 0.09906179513063011 52-53 0.104045415047722 54-55 0.1065097821653148 56-57 0.10712724608676065 58-59 0.1064356864947413 60-61 0.1043486584402543 62-63 0.10870795372566204 64-65 0.11568392389595256 66-67 0.12451365797262828 68-69 0.12919952306537852 70-71 0.12784247458024528 72-73 0.1147344015989736 74-75 0.11220691594718857 76-77 0.11409772768886053 78-79 0.11286828841415945 80-81 0.10709157039352156 82-83 0.11032570919985017 84-85 0.11625885141392103 86-87 0.13009004325430817 88-89 0.1376670116414727 90-91 0.14442755551028097 92-93 0.14356722244639974 94-95 0.13368779970326605 96-97 0.1304056359252694 98-99 0.12531773321255557 100 0.11965627512392088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.6439376E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.22408075380561 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30767421171915 27.577538908927284 2 14.379576708755287 11.280513560595193 3 5.3531762643699 6.299202542490012 4 2.6133199728001757 4.100202945945887 5 1.5776996044354845 3.094190834481231 6 1.064591322164542 2.5054569594229608 7 0.7538854829047804 2.0699325542405194 8 0.5622930185781795 1.7644341414409304 9 0.4487134350315762 1.5840334809896446 >10 2.5895974709348555 18.406274082027707 >50 0.1878448591515342 5.143821535023088 >100 0.15991496764692428 10.596851983550824 >500 0.0012205367703920541 0.30947724664803566 >1k 4.4322402968377604E-4 0.38496508331682877 >5k 2.0966017829164935E-5 0.04828150567761385 >10k+ 2.7954689747477816E-5 4.834822635222306 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1663762 4.565835594989332 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 46528 0.12768605038681233 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40025 0.10983997091497943 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.528068757269609E-4 2.7442840953149143E-6 0.0 2.7442840953149143E-6 1.92099886672044E-5 2 5.516011031582977E-4 1.0977136381259657E-5 0.0 5.488568190629829E-6 1.92099886672044E-5 3 6.860710238287286E-4 1.6465704571889485E-5 0.0 8.232852285944742E-6 2.1954272762519314E-5 4 7.903538194506953E-4 1.6465704571889485E-5 0.0 1.3721420476574572E-5 2.1954272762519314E-5 5 8.452395013569936E-4 2.1954272762519314E-5 0.0 1.3721420476574572E-5 2.1954272762519314E-5 6 9.385451605977007E-4 3.293140914377897E-5 0.0 1.3721420476574572E-5 2.1954272762519314E-5 7 9.989194106946287E-4 3.84199773344088E-5 0.0 1.3721420476574572E-5 2.1954272762519314E-5 8 0.0010592936607915569 4.1164261429723716E-5 0.0 1.3721420476574572E-5 2.1954272762519314E-5 9 0.0011279007631744297 4.1164261429723716E-5 0.0 2.4698556857834227E-5 2.4698556857834227E-5 10-11 0.0012829528145597224 5.2141397810983374E-5 0.0 2.4698556857834227E-5 2.4698556857834227E-5 12-13 0.0014366327238973576 5.76299660016132E-5 0.0 2.6070698905491687E-5 3.155926709612151E-5 14-15 0.0016232440423787717 5.900210804927066E-5 0.0 3.293140914377897E-5 3.8419977334408796E-5 16-17 0.0017577139630492026 6.449067623990048E-5 0.0 4.253640347738117E-5 4.253640347738117E-5 18-19 0.0020870280544869925 7.135138647818777E-5 0.0 4.390854552503863E-5 4.665282962035354E-5 20-21 0.002259917952491832 8.095638081178998E-5 0.0 4.5280687572696085E-5 5.351353985864083E-5 22-23 0.0024794606801170254 8.507280695476234E-5 0.0 5.076925576332591E-5 6.311853419224303E-5 24-25 0.0028705211636994004 8.918923309773471E-5 0.0 5.488568190629829E-5 6.860710238287285E-5 26-27 0.004072517597447332 9.193351719304963E-5 0.0 6.174639214458558E-5 7.135138647818777E-5 28-29 0.00734370423906271 9.742208538367946E-5 0.0 6.586281828755794E-5 7.135138647818777E-5 30-31 0.015967617008589827 1.0702707971728166E-4 0.0 6.72349603352154E-5 7.135138647818777E-5 32-33 0.034479185373536586 1.2074850019385623E-4 0.0 7.135138647818777E-5 9.056137514539217E-5 34-35 0.06876215443425815 1.4133063090871807E-4 0.0 8.370066490710488E-5 9.6049943336022E-5 36-37 0.1256264651732785 1.509356252423203E-4 0.0 9.193351719304963E-5 1.0428279562196675E-4 38-39 0.21094488555457153 1.5779633548060757E-4 0.0 1.0153851152665182E-4 1.0839922176493911E-4 40-41 0.3280558371800878 1.6191276162357994E-4 1.3721420476574571E-6 1.0428279562196675E-4 1.0977136381259657E-4 42-43 0.4793729179116569 1.6740132981420976E-4 2.7442840953149143E-6 1.0839922176493911E-4 1.1114350586025404E-4 44-45 0.6719146343230467 1.701456139095247E-4 2.7442840953149143E-6 1.1937635814619876E-4 1.1251564790791149E-4 46-47 0.9100704688247132 1.7700632414781195E-4 2.7442840953149143E-6 1.2486492633682862E-4 1.1251564790791149E-4 48-49 1.2003773061316965 1.8661131848141417E-4 2.7442840953149143E-6 1.3172563657511588E-4 1.1663207405088386E-4 50-51 1.5367606733990176 1.893556025767291E-4 2.7442840953149143E-6 1.3858634681340317E-4 1.1800421609854131E-4 52-53 1.9175451851864862 1.9896059691033128E-4 2.7442840953149143E-6 1.4819134114700538E-4 1.221206422415137E-4 54-55 2.344558534701582 2.0582130714861857E-4 2.7442840953149143E-6 1.536799093376352E-4 1.2623706838448605E-4 56-57 2.8263368176227823 2.1679844352987822E-4 2.7442840953149143E-6 1.5916847752826504E-4 1.3035349452745842E-4 58-59 3.367103487172777 2.2503129581582298E-4 2.7442840953149143E-6 1.605406195759225E-4 1.344699206704308E-4 60-61 3.9555164171856294 2.3189200605411027E-4 2.7442840953149143E-6 1.6465704571889486E-4 1.3858634681340317E-4 62-63 4.581634438526061 2.4149700038771245E-4 2.7442840953149143E-6 1.6740132981420976E-4 1.44074915004033E-4 64-65 5.242582913604228 2.565905629119445E-4 2.7442840953149143E-6 1.6877347186186723E-4 1.5505205138529265E-4 66-67 5.948112283810787 2.6070698905491683E-4 2.7442840953149143E-6 1.7151775595718215E-4 1.6740132981420976E-4 68-69 6.695763670596335 2.6070698905491683E-4 2.7442840953149143E-6 1.7563418210015451E-4 1.7151775595718215E-4 70-71 7.4768088783957225 2.6070698905491683E-4 2.7442840953149143E-6 1.7700632414781198E-4 1.728898980048396E-4 72-73 8.285486282750835 2.661955572455467E-4 2.7442840953149143E-6 1.824948923384418E-4 1.7700632414781198E-4 74-75 9.123601622596391 2.6756769929320415E-4 2.7442840953149143E-6 1.8661131848141417E-4 1.7975060824312688E-4 76-77 9.989044543463093 2.7717269362680634E-4 2.7442840953149143E-6 1.8661131848141417E-4 1.8386703438609927E-4 78-79 10.879995584995747 2.812891197697787E-4 2.7442840953149143E-6 1.8661131848141417E-4 1.8661131848141417E-4 80-81 11.789149462932624 2.88149830008066E-4 2.7442840953149143E-6 1.8661131848141417E-4 1.8661131848141417E-4 82-83 12.718895076578699 2.908941141033809E-4 2.7442840953149143E-6 1.8661131848141417E-4 1.8661131848141417E-4 84-85 13.663955167618678 2.991269663893257E-4 2.7442840953149143E-6 1.893556025767291E-4 1.8798346052907163E-4 86-87 14.623292396664532 3.1147624481824276E-4 2.7442840953149143E-6 1.9621631281501638E-4 1.9484417076735892E-4 88 15.353621862240452 3.128483868659002E-4 2.7442840953149143E-6 1.9758845486267382E-4 1.9484417076735892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 214410 0.0 84.43026 8 AGTGGTA 216495 0.0 83.73324 5 GTGGTAT 217760 0.0 83.190025 6 TGGTATC 217680 0.0 82.92784 7 GTATCAA 218190 0.0 82.86708 9 AAGCAGT 226055 0.0 80.733795 1 GCAGTGG 228760 0.0 79.82073 3 CAGTGGT 231610 0.0 78.26282 4 AGCAGTG 237910 0.0 76.807816 2 CAACGCA 210785 0.0 44.64366 12-13 ATCAACG 212525 0.0 44.333828 10-11 ACGCAGA 214175 0.0 43.968174 14-15 AGAGTAC 214025 0.0 43.303402 18-19 TCAACGC 210410 0.0 43.147114 12-13 AACGCAG 213070 0.0 42.700264 14-15 GAGTACT 209140 0.0 42.474037 20-21 CGCAGAG 215990 0.0 42.040585 16-17 AGTACTT 221805 0.0 41.506756 20-21 TATCAAC 218420 0.0 41.351242 10-11 CAGAGTA 225865 0.0 39.722103 18-19 >>END_MODULE