FastQCFastQC Report
Thu 26 May 2016
SRR1660744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36439376
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16212894.449277616609022No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT502340.1378563672440494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2178100.084.006188
AGTGGTA2186850.082.996415
TGGTATC2212750.082.639897
GTGGTAT2196750.082.588066
GTATCAA2218050.082.38729
AAGCAGT2159950.081.653011
GCAGTGG2242400.080.855583
CAGTGGT2332250.077.669914
AGCAGTG2330100.077.520282
CAACGCA2132950.044.4689312-13
ATCAACG2148850.044.2109110-11
ACGCAGA2169900.043.84595514-15
AGAGTAC2193250.043.1355118-19
TCAACGC2127300.042.89132712-13
AACGCAG2154300.042.4704814-15
GAGTACT2143400.042.200120-21
CGCAGAG2185300.041.83912316-17
AGTACTT2273300.041.3838620-21
TATCAAC2213800.041.2727110-11
GCAGAGT2402900.039.6960716-17
Produced by FastQC (version 0.11.5)