##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660744_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36439376 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.156085246904336 34.0 31.0 34.0 27.0 34.0 2 31.821518129179818 34.0 31.0 34.0 28.0 34.0 3 31.584610449970384 34.0 31.0 34.0 28.0 34.0 4 35.531449934817765 37.0 35.0 37.0 33.0 37.0 5 35.875115891117346 37.0 35.0 37.0 33.0 37.0 6 35.33014464901924 37.0 35.0 37.0 33.0 37.0 7 35.557925443070154 37.0 35.0 37.0 33.0 37.0 8 35.91933887671402 37.0 35.0 37.0 33.0 37.0 9 37.722547965695135 39.0 37.0 39.0 35.0 39.0 10-11 37.75713579727601 39.0 37.5 39.0 35.0 39.0 12-13 37.81379331248702 39.0 38.0 39.0 35.0 39.0 14-15 39.25232534168532 41.0 39.0 41.0 36.0 41.0 16-17 39.2192405133392 41.0 39.0 41.0 36.0 41.0 18-19 39.21655298103897 41.0 39.0 41.0 36.0 41.0 20-21 39.16173939147586 40.5 39.0 41.0 36.0 41.0 22-23 39.0550395401941 40.0 39.0 41.0 35.5 41.0 24-25 39.05598479512931 40.0 39.0 41.0 35.5 41.0 26-27 38.99345956143706 40.0 39.0 41.0 35.0 41.0 28-29 38.90942513669828 40.0 38.5 41.0 35.0 41.0 30-31 38.81293754865616 40.0 38.0 41.0 35.0 41.0 32-33 38.69011391687937 40.0 38.0 41.0 35.0 41.0 34-35 38.60307754721157 40.0 38.0 41.0 34.0 41.0 36-37 38.48339059099146 40.0 38.0 41.0 34.0 41.0 38-39 38.13098004202926 40.0 37.0 41.0 34.0 41.0 40-41 37.998887974920315 40.0 37.0 41.0 33.0 41.0 42-43 37.837549029379645 40.0 37.0 41.0 33.0 41.0 44-45 37.71207753392923 40.0 36.5 41.0 33.0 41.0 46-47 37.55719156661739 40.0 36.0 41.0 33.0 41.0 48-49 37.60802904528332 40.0 36.0 41.0 33.0 41.0 50-51 37.61664438216505 40.0 36.0 41.0 33.0 41.0 52-53 37.389307503509386 40.0 36.0 41.0 32.5 41.0 54-55 36.93289017901953 40.0 36.0 41.0 31.0 41.0 56-57 36.518842432976896 39.0 35.0 41.0 30.0 41.0 58-59 36.3994625895899 39.0 35.0 41.0 30.0 41.0 60-61 36.254050878917354 39.0 35.0 41.0 29.0 41.0 62-63 36.01767312096672 39.0 35.0 41.0 29.0 41.0 64-65 35.7469884912409 38.0 35.0 40.5 29.0 41.0 66-67 35.45515181160073 37.5 35.0 40.0 28.5 41.0 68-69 35.17518513489364 37.0 35.0 40.0 28.0 41.0 70-71 34.861307065192335 36.5 34.0 39.0 28.0 41.0 72-73 34.51413077161365 36.0 34.0 39.0 28.0 41.0 74-75 34.133231438979635 35.5 34.0 38.5 27.0 40.0 76-77 32.42414859134799 34.5 32.0 36.5 25.0 39.0 78-79 33.3655828381913 35.0 33.5 37.0 26.0 39.0 80-81 33.27016313341919 35.0 34.0 36.5 26.0 39.0 82-83 33.017418835602456 35.0 34.0 36.0 26.0 38.0 84-85 32.77378373603324 35.0 34.0 36.0 26.0 37.0 86-87 32.56475353749197 35.0 34.0 35.0 26.0 37.0 88-89 32.39551589741822 35.0 33.0 35.0 25.0 36.0 90-91 32.238881656480615 35.0 33.0 35.0 25.0 36.0 92-93 32.098304331556065 35.0 33.0 35.0 25.0 36.0 94-95 31.997791866688388 35.0 33.0 35.0 25.0 36.0 96-97 31.90040578631204 35.0 33.0 35.0 24.0 35.0 98-99 31.809244400892048 35.0 33.0 35.0 24.0 35.0 100 31.75644942438092 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6119273242786214 1101 2 1.2054655654424344 1101 3 1.5728004219697027 1101 4 0.9757896821998173 1101 5 0.5856804975812651 1101 6 1.1261705816593732 1101 7 0.8227570473769674 1101 8 0.4991070119831491 1101 9 0.511862860150309 1101 10-11 0.42052297048450527 1101 12-13 0.41150506189315905 1101 14-15 0.5312958158231353 1101 16-17 0.5433918740496608 1101 18-19 0.5172923297205045 1101 20-21 0.5282545591616739 1101 22-23 0.4998097913952151 1101 24-25 0.5102803434799199 1101 26-27 0.5201917496671058 1101 28-29 0.49444894978130804 1101 30-31 0.5123855388576786 1101 32-33 0.5558467561693874 1101 34-35 0.5513882006383923 1101 36-37 0.5185773671964995 1101 38-39 0.5563975971178081 1101 40-41 0.553234702228977 1101 42-43 0.5131473095464614 1101 44-45 0.4910466147806005 1101 46-47 0.5108761182798673 1101 48-49 0.4709347797571084 1101 50-51 0.46562648748915336 1101 52-53 0.45467584308793363 1101 54-55 0.4756417522578502 1101 56-57 0.44631859725772927 1101 58-59 0.44052313618616523 1101 60-61 0.4656913200748036 1101 62-63 0.462959204678711 1101 64-65 0.4275644023721483 1101 66-67 0.4567533310465208 1101 68-69 0.4375287720007748 1101 70-71 0.414336056898982 1101 72-73 0.4074724878402094 1101 74-75 0.4049928156828102 1101 76-77 0.3007380342549837 1101 78-79 0.40775613016440104 1101 80-81 0.4108530949542626 1101 82-83 0.4031709805453687 1101 84-85 0.39331538419575196 1101 86-87 0.3824668240564435 1101 88-89 0.33890947903255864 1101 90-91 0.36810567215567147 1101 92-93 0.35907587127658047 1101 94-95 0.3238272996640674 1101 96-97 0.3622057973096915 1101 98-99 0.3584725147310799 1101 100 0.38003797272858364 1102 1 1.5172828332487462 1102 2 1.1478270098077452 1102 3 1.515418052131409 1102 4 0.9263241570892973 1102 5 0.5144339858398084 1102 6 1.0809312620021458 1102 7 0.7872756122429863 1102 8 0.45636799650876014 1102 9 0.4742950560657704 1102 10-11 0.3761905952357054 1102 12-13 0.36055321992895273 1102 14-15 0.4719789434456132 1102 16-17 0.4820688410813432 1102 18-19 0.42468297376328223 1102 20-21 0.42149783937415464 1102 22-23 0.4380365148546801 1102 24-25 0.4350518608695495 1102 26-27 0.42486469419129946 1102 28-29 0.4300841212377762 1102 30-31 0.4465541660481307 1102 32-33 0.4563667330363117 1102 34-35 0.42876578399165766 1102 36-37 0.42020460194189013 1102 38-39 0.45515263849483745 1102 40-41 0.4508780160351691 1102 42-43 0.4535632165998109 1102 44-45 0.4321370352084415 1102 46-47 0.4633321343038901 1102 48-49 0.428812063840212 1102 50-51 0.35762108050676744 1102 52-53 0.3719598335677645 1102 54-55 0.4306075814012047 1102 56-57 0.4713237555686547 1102 58-59 0.44936141490712345 1102 60-61 0.47580765892434584 1102 62-63 0.41671881690798074 1102 64-65 0.3649570395180177 1102 66-67 0.34238171100819415 1102 68-69 0.3394787808801496 1102 70-71 0.33277811958883063 1102 72-73 0.3220374100021175 1102 74-75 0.3198794478778595 1102 76-77 0.18138812149906158 1102 78-79 0.38465694992707 1102 80-81 0.41651477949135085 1102 82-83 0.39259220549664775 1102 84-85 0.3925501105186697 1102 86-87 0.3854275534284781 1102 88-89 0.40586409701556647 1102 90-91 0.41989369816533184 1102 92-93 0.39549408783192774 1102 94-95 0.3557684595369963 1102 96-97 0.3689119249611821 1102 98-99 0.3941746804772599 1102 100 0.42059173756334545 1103 1 1.5325538232816918 1103 2 1.1361533126326258 1103 3 1.479985641703152 1103 4 0.913922315794153 1103 5 0.5190322151061793 1103 6 1.039598039611782 1103 7 0.7631681306459939 1103 8 0.43688774492108706 1103 9 0.43996298041887627 1103 10-11 0.3658970447562879 1103 12-13 0.33035192173937133 1103 14-15 0.4124414571698978 1103 16-17 0.4447919566235612 1103 18-19 0.3869500369574581 1103 20-21 0.40613613191101194 1103 22-23 0.38034629128565456 1103 24-25 0.3822232072374874 1103 26-27 0.3949303104850088 1103 28-29 0.37269173449545434 1103 30-31 0.3896512786418356 1103 32-33 0.4301381429914315 1103 34-35 0.42711952347952575 1103 36-37 0.36526213048270506 1103 38-39 0.410063245769301 1103 40-41 0.4546821894283468 1103 42-43 0.46428537342406173 1103 44-45 0.42493972990327933 1103 46-47 0.46096962399307273 1103 48-49 0.424934795721299 1103 50-51 0.40772796915826603 1103 52-53 0.40051085666406294 1103 54-55 0.4416342358506853 1103 56-57 0.46661676789282325 1103 58-59 0.4369759741614061 1103 60-61 0.444479238157264 1103 62-63 0.4035309954959345 1103 64-65 0.3817668705614352 1103 66-67 0.38036527796509034 1103 68-69 0.35259775136862714 1103 70-71 0.38008962635998245 1103 72-73 0.3507161600824631 1103 74-75 0.389046672890494 1103 76-77 0.2514120300650191 1103 78-79 0.38100539434950065 1103 80-81 0.41473601905664736 1103 82-83 0.41992146826320464 1103 84-85 0.3911517171874621 1103 86-87 0.3465586663582485 1103 88-89 0.38082377318291805 1103 90-91 0.3954969358980307 1103 92-93 0.3888670372833829 1103 94-95 0.3868069232175415 1103 96-97 0.4095628704638017 1103 98-99 0.42419529734374706 1103 100 0.38046100219272105 1104 1 1.4374863109607396 1104 2 1.0568355226266597 1104 3 1.4368531229012262 1104 4 0.8998025693735698 1104 5 0.48365525525342434 1104 6 1.0279264843948681 1104 7 0.7702722371144617 1104 8 0.43184269345458404 1104 9 0.4164166174731889 1104 10-11 0.34183683321893454 1104 12-13 0.3094654116994704 1104 14-15 0.3773041278285163 1104 16-17 0.398736596041239 1104 18-19 0.37803924642521736 1104 20-21 0.3594031753704954 1104 22-23 0.35666708945931447 1104 24-25 0.35666918526752767 1104 26-27 0.35644133298346503 1104 28-29 0.3048820180874472 1104 30-31 0.33844836880473395 1104 32-33 0.3797385162426181 1104 34-35 0.3758541146018928 1104 36-37 0.39555447638597485 1104 38-39 0.41474375489914905 1104 40-41 0.38146799872183834 1104 42-43 0.41732884839424855 1104 44-45 0.39537195844355466 1104 46-47 0.3973561079842298 1104 48-49 0.3468014326348765 1104 50-51 0.3643034256934996 1104 52-53 0.3870233969899388 1104 54-55 0.3634091888576165 1104 56-57 0.43438139347078675 1104 58-59 0.4584556781349747 1104 60-61 0.43000900310843804 1104 62-63 0.3704087815668373 1104 64-65 0.37328200955446533 1104 66-67 0.3981077540071354 1104 68-69 0.38894777658897084 1104 70-71 0.39985819573150394 1104 72-73 0.41460996248979143 1104 74-75 0.4047227407503371 1104 76-77 0.26758584261303753 1104 78-79 0.4192666501212159 1104 80-81 0.44351758208300396 1104 82-83 0.4532702192763338 1104 84-85 0.4255070481750849 1104 86-87 0.4172458051469121 1104 88-89 0.40109440352152603 1104 90-91 0.4135288743576382 1104 92-93 0.3956987106176584 1104 94-95 0.39649673353705595 1104 96-97 0.4088491044174205 1104 98-99 0.39548614479473443 1104 100 0.40354043021561026 1105 1 1.3758046370757917 1105 2 1.0111910573797296 1105 3 1.365371123305529 1105 4 0.842430805640916 1105 5 0.43976782152326876 1105 6 0.993892585085824 1105 7 0.7281607997882276 1105 8 0.3540762856902262 1105 9 0.35677337140417364 1105 10-11 0.26836715798582134 1105 12-13 0.2516649704740601 1105 14-15 0.31726685405202915 1105 16-17 0.33354776509113293 1105 18-19 0.30214869942730616 1105 20-21 0.3293115483474125 1105 22-23 0.322080172934065 1105 24-25 0.29812659924669305 1105 26-27 0.32267466051338545 1105 28-29 0.310814355651182 1105 30-31 0.3112619585879983 1105 32-33 0.366781108860323 1105 34-35 0.37829728660128836 1105 36-37 0.36896638736845944 1105 38-39 0.3888472084277055 1105 40-41 0.3814600547822522 1105 42-43 0.38736337639091545 1105 44-45 0.3463358830855512 1105 46-47 0.3595610416043584 1105 48-49 0.36127360444245227 1105 50-51 0.3555765190534359 1105 52-53 0.3691256226677595 1105 54-55 0.38141080643194414 1105 56-57 0.39435753766334614 1105 58-59 0.3461021508643327 1105 60-61 0.2982607532893411 1105 62-63 0.2698727824651286 1105 64-65 0.25584829831657885 1105 66-67 0.27218301393126865 1105 68-69 0.2678924961803588 1105 70-71 0.275330832457918 1105 72-73 0.2711736448831559 1105 74-75 0.28257970112225905 1105 76-77 0.1910572316519321 1105 78-79 0.29299444572595235 1105 80-81 0.2991240935669808 1105 82-83 0.2863727862390846 1105 84-85 0.2913121456931762 1105 86-87 0.28900010841405077 1105 88-89 0.3156871529836067 1105 90-91 0.2998833704936672 1105 92-93 0.28483793816964464 1105 94-95 0.28951448349044284 1105 96-97 0.2873867547698552 1105 98-99 0.31525537427225814 1105 100 0.2707171467039764 1106 1 1.3468876264994627 1106 2 0.9889964598831789 1106 3 1.3443417125723087 1106 4 0.7872826128196806 1106 5 0.3995565356341828 1106 6 0.9300753294298687 1106 7 0.6677862706624822 1106 8 0.32293709828722683 1106 9 0.3044415054562677 1106 10-11 0.2060959649770524 1106 12-13 0.18367805882913046 1106 14-15 0.23160386396380517 1106 16-17 0.22985182101079715 1106 18-19 0.2069552826586687 1106 20-21 0.23502835238083009 1106 22-23 0.246404550255221 1106 24-25 0.21884335413005118 1106 26-27 0.19748275510923463 1106 28-29 0.22774505544212076 1106 30-31 0.2168564992752664 1106 32-33 0.2668028180855728 1106 34-35 0.269939056968191 1106 36-37 0.24120500233192388 1106 38-39 0.2562311306940117 1106 40-41 0.2528085927905366 1106 42-43 0.2937770041884349 1106 44-45 0.2915742017662737 1106 46-47 0.27391482525570865 1106 48-49 0.2144640541855125 1106 50-51 0.22245976087292973 1106 52-53 0.23951943346633442 1106 54-55 0.25014397477465167 1106 56-57 0.27093061492688975 1106 58-59 0.2275405725656512 1106 60-61 0.2052460091402608 1106 62-63 0.16488208488286205 1106 64-65 0.1500628045076695 1106 66-67 0.16518251516050242 1106 68-69 0.16319122208268055 1106 70-71 0.1633767444038341 1106 72-73 0.10752068611837018 1106 74-75 0.1476684587156214 1106 76-77 0.03952745380357214 1106 78-79 0.15551284180165226 1106 80-81 0.20632563530212167 1106 82-83 0.21957595125750373 1106 84-85 0.19076012262786435 1106 86-87 0.1880370896908019 1106 88-89 0.19042848412811963 1106 90-91 0.20331814725688702 1106 92-93 0.20048921569502198 1106 94-95 0.15752765074160635 1106 96-97 0.18575025344349427 1106 98-99 0.20650939030614168 1106 100 0.22625901106525603 1107 1 1.3002802795402424 1107 2 0.9188601802480925 1107 3 1.2855223405350031 1107 4 0.7918330213025158 1107 5 0.39429473541999727 1107 6 0.9223956812135654 1107 7 0.6421326190509546 1107 8 0.28414490804956216 1107 9 0.24127373320410328 1107 10-11 0.1695462540975683 1107 12-13 0.1049188477704277 1107 14-15 0.16041520926345498 1107 16-17 0.1888934385584804 1107 18-19 0.1391803219015486 1107 20-21 0.13477687733429633 1107 22-23 0.1788548365033904 1107 24-25 0.16021417141413252 1107 26-27 0.14080765749962154 1107 28-29 0.15539581746711661 1107 30-31 0.11778925837406717 1107 32-33 0.17328608743583374 1107 34-35 0.1928075925734518 1107 36-37 0.1718793618830432 1107 38-39 0.172524189424081 1107 40-41 0.19756372417885615 1107 42-43 0.2053371784575475 1107 44-45 0.15793903533013776 1107 46-47 0.2237892491644473 1107 48-49 0.20449951934661215 1107 50-51 0.17836741567028724 1107 52-53 0.18333214484650284 1107 54-55 0.2483253203087159 1107 56-57 0.23702425388535886 1107 58-59 0.2013874959383557 1107 60-61 0.19681906954541972 1107 62-63 0.15350117824376497 1107 64-65 0.14602003382027817 1107 66-67 0.15441949435044933 1107 68-69 0.16528145041107223 1107 70-71 0.1601289770033958 1107 72-73 0.16496977568541382 1107 74-75 0.15994714400529375 1107 76-77 -0.006838235928022129 1107 78-79 0.12985874187710778 1107 80-81 0.21474219920364135 1107 82-83 0.23113213766444574 1107 84-85 0.2092230613010031 1107 86-87 0.1868921002368964 1107 88-89 0.21526304506674165 1107 90-91 0.19815448040041161 1107 92-93 0.1880658071015162 1107 94-95 0.1561177311094042 1107 96-97 0.19662736558803573 1107 98-99 0.2369580295278304 1107 100 0.23418047302548572 1108 1 1.2715448654951977 1108 2 0.8958677754692452 1108 3 1.2423332977162787 1108 4 0.7627939637717063 1108 5 0.3398389104450743 1108 6 0.8567550624169442 1108 7 0.5994955569816582 1108 8 0.26430334444452086 1108 9 0.19379068727648274 1108 10-11 0.13332160165374063 1108 12-13 0.11878527965077978 1108 14-15 0.12878101686145982 1108 16-17 0.1338023813292324 1108 18-19 0.10561347298832402 1108 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-0.8761812460955056 2316 94-95 -0.9227133643609662 2316 96-97 -0.9942957322096397 2316 98-99 -1.15063991506727 2316 100 -1.0794976110971213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 76.0 7 985.0 8 1194.0 9 507.0 10 475.0 11 880.0 12 1205.0 13 1832.0 14 3953.0 15 9444.0 16 20073.0 17 32711.0 18 42657.0 19 54428.0 20 72384.0 21 102138.0 22 153418.0 23 248775.0 24 417629.0 25 557802.0 26 569524.0 27 491876.0 28 453802.0 29 484326.0 30 561388.0 31 684131.0 32 843596.0 33 1075423.0 34 1465832.0 35 2237256.0 36 4017880.0 37 8169383.0 38 1.0631659E7 39 3024877.0 40 5857.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.968302592891073 32.31213151874369 27.269065347198723 24.450500541166516 2 20.881873839884157 31.983390332939283 29.2550827461707 17.879653081005856 3 24.095529078246102 26.32075697549523 27.393550215488972 22.1901637307697 4 18.966228584130782 28.540611595785847 25.864617951595953 26.62854186848742 5 21.79230620511636 31.992088775421855 26.745402945413566 19.470202074048217 6 27.6269843949021 27.53764734328553 26.131681787630257 18.70368647418211 7 20.88447540826271 26.696519210644205 31.919046671937366 20.49995870915572 8 25.408651723945923 26.711300917271117 27.06188961302502 20.818157745757937 9 25.021771092396616 25.159229542219098 28.766761230125383 21.052238135258904 10-11 20.591677153613215 29.44048382204199 29.320986625213674 20.64685239913112 12-13 20.585088528344524 26.949267474390926 29.018228619703667 23.447415377560883 14-15 20.536279216197336 32.25891409336977 26.443299962106927 20.761506728325973 16-17 23.370495619544602 26.726855151854746 26.460531883077426 23.44211734552323 18-19 20.608794179131937 29.45374668325824 26.505772217394725 23.4316869202151 20-21 23.29848117358498 29.570114952685778 26.462810585057824 20.66859328867142 22-23 23.265482355936644 26.898673898479082 29.119246226203654 20.71659751938062 24-25 20.697367408189837 29.399775269397495 26.305747507799637 23.59710981461303 26-27 20.74654164501234 26.74546129114829 31.7702401892351 20.737756874604276 28-29 20.7214294456359 26.86923507243258 31.737023569431265 20.67231191250026 30-31 20.695986639852766 26.77686695040687 31.726416894018257 20.800729515722107 32-33 20.840189777114198 26.90659090732868 31.52066038691964 20.732558928637474 34-35 20.82976626943761 27.025069602945358 31.430249253491315 20.714914874125718 36-37 20.75570054653837 26.983379629483515 31.475984298051063 20.784935525927057 38-39 20.79890004300386 26.961473360994546 31.517651583744623 20.721975012256973 40-41 20.825724280844536 27.02003344739994 31.47444246214251 20.67979980961302 42-43 20.82517570716029 27.033515270635654 31.418472104071586 20.72283691813247 44-45 20.86041574124116 27.11609543234448 31.382904909078686 20.64058391733568 46-47 20.905284561443786 27.223088689080612 31.26488606755012 20.606740681925483 48-49 20.9325542183116 27.243523986616086 31.149487588399992 20.674434206672316 50-51 20.950363645740364 27.337773357163798 31.07471860281364 20.6371443942822 52-53 21.06546243968001 27.484174337764745 30.792699699979863 20.65766352257538 54-55 21.325630757135865 27.690159408770782 30.14867176652407 20.83553806756928 56-57 21.707499635477923 28.19451995956001 29.16438748872397 20.933592916238098 58-59 21.803211534309835 28.668428349991675 28.54425433718715 20.984105778511335 60-61 21.83722268282744 28.911017732346362 28.232769304050738 21.018990280775462 62-63 21.810841485041436 29.05015695635429 28.0768778219688 21.062123736635474 64-65 21.849779468110285 29.13065664386345 27.95906562205055 21.060498265975717 66-67 21.785806763157957 29.040893927624918 28.042490736850894 21.13080857236623 68-69 21.741623920370206 28.93004299457361 28.19133850323652 21.136994581819664 70-71 21.707224078798145 28.873960309854436 28.229080569292996 21.18973504205442 72-73 21.671786436135154 28.82254273034843 28.188803055933914 21.316867777582495 74-75 21.747856593831095 28.903868433776335 27.997203066110426 21.351071906282147 76-77 21.778261168810356 29.045632357861816 27.74713129705273 21.4289751762751 78-79 21.856499055329913 29.215369045996752 27.36510125195523 21.5630306467181 80-81 21.91795246266649 29.409764945089695 27.021448684200323 21.65083390804349 82-83 21.956650224638107 29.55460181975887 26.653748763429576 21.834999192173445 84-85 21.95013671620329 29.607851686497817 26.445337254946427 21.996674342352463 86-87 21.985559924148284 29.645685975819674 26.289311070502862 22.079443029529184 88-89 21.98865175173848 29.682730021849746 26.11378001868834 22.21483820772343 90-91 22.024205986653804 29.667178255416825 25.950871608395047 22.35774414953432 92-93 22.04574718301847 29.68053746543074 25.87089516947694 22.402820182073853 94-95 22.083604261537882 29.701873082250213 25.750128811762963 22.464393844448942 96-97 22.09529323807579 29.663112773811218 25.675258889434012 22.566335098678987 98-99 22.073985232704732 29.649333090732515 25.65770809053689 22.618973586025863 100 22.041454322842313 29.623828714387734 25.573536563152306 22.76118039961765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31455.0 1 24337.0 2 13750.0 3 9634.5 4 8767.5 5 8782.0 6 9174.0 7 9429.5 8 9625.5 9 9970.0 10 11134.5 11 20680.0 12 141340.0 13 165489.5 14 76162.0 15 80569.0 16 92481.5 17 103919.5 18 114076.5 19 124198.0 20 137123.0 21 157060.0 22 186535.0 23 220475.5 24 254969.5 25 294073.5 26 340767.0 27 397337.5 28 465601.5 29 534552.0 30 593766.0 31 650542.5 32 710924.0 33 764688.5 34 808994.5 35 861794.5 36 923683.0 37 980988.0 38 1038781.0 39 1083581.0 40 1108732.5 41 1143364.5 42 1187691.5 43 1205695.5 44 1210969.0 45 1231187.5 46 1250639.5 47 1260632.5 48 1261516.0 49 1256296.0 50 1246186.5 51 1228345.0 52 1195365.0 53 1138260.5 54 1062827.5 55 982621.0 56 901854.0 57 810816.0 58 707952.5 59 602249.0 60 493975.5 61 392149.5 62 302828.0 63 223770.0 64 161190.5 65 115281.0 66 81586.0 67 58489.5 68 41641.5 69 30142.5 70 22538.0 71 16822.5 72 13141.5 73 10197.0 74 7967.0 75 6578.0 76 4927.0 77 2880.0 78 1573.5 79 967.0 80 615.0 81 436.0 82 310.0 83 213.0 84 138.0 85 94.5 86 74.5 87 49.5 88 30.5 89 27.0 90 18.0 91 11.5 92 9.0 93 5.5 94 4.5 95 2.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3284523862318608 2 0.006262456305508635 3 2.4493970478528504 4 7.574224103069164E-4 5 3.0187125048464057E-5 6 0.008798174809579616 7 0.007483662727923771 8 2.6070698905491683E-4 9 2.7442840953149143E-6 10-11 7.546781262116014E-5 12-13 4.116426142972371E-6 14-15 0.0 16-17 3.84199773344088E-5 18-19 0.0 20-21 3.3754694372373445E-4 22-23 2.4698556857834227E-5 24-25 5.241582622051487E-4 26-27 0.003608733585339112 28-29 0.006272061299842237 30-31 0.005634015247681519 32-33 0.015322710246190824 34-35 0.009872562032895405 36-37 0.005107112701381055 38-39 0.005355470412007055 40-41 0.008400253615758952 42-43 0.019082379456772255 44-45 0.03413614986162222 46-47 0.03311664832021273 48-49 0.039679603734158346 50-51 0.03998833569488128 52-53 0.06216763975321642 54-55 0.03315918472369011 56-57 0.022261632581194582 58-59 0.017095517771764258 60-61 0.018205580688319144 62-63 0.024768536102264756 64-65 0.04472908646953779 66-67 0.04765998188333412 68-69 0.050452290950317044 70-71 0.06113167250723504 72-73 0.0685412395645853 74-75 0.059386307822614746 76-77 0.05942609994199681 78-79 0.05429017225761495 80-81 0.05171191735006659 82-83 0.04822393226492133 84-85 0.05024509750112076 86-87 0.05822135922415356 88-89 0.06482959532567188 90-91 0.06065965564284086 92-93 0.06507520875220256 94-95 0.07339999455534035 96-97 0.0729499319637087 98-99 0.047764264678956087 100 0.007126905795532832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.6439376E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.4029174579882 #Duplication Level Percentage of deduplicated Percentage of total 1 68.6747161724177 26.373094566201242 2 15.350326212551346 11.789946209876039 3 5.572882199554083 6.420448053377015 4 2.7115166738289616 4.165206040440493 5 1.65189474363678 3.1718788744583915 6 1.1482852225151008 2.6458501571085016 7 0.7917267565562045 2.128323209691605 8 0.5914414846816608 1.817046281396785 9 0.4543089282717823 1.5702109445563501 >10 2.6931775264443334 18.755723421797335 >50 0.19407666869380877 5.241377138100827 >100 0.1640801074390535 10.41005459832349 >500 0.0010481162977004853 0.2587423094096221 >1k 4.5472869208260894E-4 0.35616743981083754 >5k 2.8648186723933283E-5 0.06975652915715959 >10k+ 3.581023268483598E-5 4.826174226294279 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1621289 4.449277616609022 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 50234 0.1378563672440494 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.034097620112924E-4 5.488568190629829E-6 0.0 0.0 1.3721420476574572E-5 2 4.912268530613697E-4 2.4698556857834227E-5 0.0 1.0977136381259657E-5 1.3721420476574572E-5 3 5.872767963973917E-4 3.84199773344088E-5 0.0 1.3721420476574572E-5 1.3721420476574572E-5 4 6.72349603352154E-4 4.665282962035354E-5 0.0 1.92099886672044E-5 1.3721420476574572E-5 5 7.299795693537672E-4 4.665282962035354E-5 0.0 1.92099886672044E-5 1.3721420476574572E-5 6 8.068195240225848E-4 5.488568190629829E-5 0.0 1.92099886672044E-5 1.6465704571889485E-5 7 8.507280695476234E-4 5.488568190629829E-5 0.0 2.1954272762519314E-5 2.1954272762519314E-5 8 8.836594786914025E-4 5.488568190629829E-5 0.0 2.4698556857834227E-5 2.1954272762519314E-5 9 9.6049943336022E-4 5.76299660016132E-5 0.0 2.4698556857834227E-5 2.1954272762519314E-5 10-11 0.0011306450472697445 6.174639214458558E-5 0.0 2.4698556857834227E-5 2.88149830008066E-5 12-13 0.0013186285077988163 6.311853419224303E-5 0.0 3.293140914377897E-5 4.116426142972371E-5 14-15 0.0014819134114700536 6.586281828755794E-5 0.0 4.1164261429723716E-5 5.2141397810983374E-5 16-17 0.0016616640197131805 6.860710238287285E-5 0.0 4.9397113715668455E-5 5.76299660016132E-5 18-19 0.001984117400912683 7.546781262116014E-5 0.0 5.2141397810983374E-5 6.449067623990048E-5 20-21 0.002150146588679235 8.507280695476234E-5 0.0 5.351353985864083E-5 6.860710238287285E-5 22-23 0.00240124858340055 9.056137514539217E-5 0.0 5.488568190629829E-5 6.99792444305303E-5 24-25 0.002845822606841566 9.330565924070708E-5 0.0 5.6257823953955744E-5 8.095638081178998E-5 26-27 0.004032725478065267 9.6049943336022E-5 0.0 6.311853419224303E-5 8.644494900241981E-5 28-29 0.00734919280725334 9.6049943336022E-5 0.0 6.72349603352154E-5 9.193351719304963E-5 30-31 0.016106203355403234 1.0428279562196675E-4 0.0 7.409567057350269E-5 9.879422743133691E-5 32-33 0.034742636646686816 1.1251564790791149E-4 0.0 7.409567057350269E-5 1.0428279562196675E-4 34-35 0.06950311113999318 1.2074850019385623E-4 0.0 7.546781262116016E-5 1.1114350586025404E-4 36-37 0.1269670479538398 1.2760921043214352E-4 0.0 7.68399546688176E-5 1.2074850019385623E-4 38-39 0.2131293356944422 1.3035349452745842E-4 0.0 7.68399546688176E-5 1.2760921043214352E-4 40-41 0.3314107244866103 1.3172563657511588E-4 0.0 7.821209671647505E-5 1.3035349452745842E-4 42-43 0.4839668494872141 1.3584206271808827E-4 0.0 8.370066490710488E-5 1.344699206704308E-4 44-45 0.6777819137188299 1.4819134114700538E-4 0.0 8.781709105007726E-5 1.5230776728997775E-4 46-47 0.917473175171825 1.605406195759225E-4 0.0 8.918923309773471E-5 1.6191276162357994E-4 48-49 1.2093758136802344 1.660291877665523E-4 0.0 9.193351719304963E-5 1.632849036712374E-4 50-51 1.5476568533994655 1.7288989800483962E-4 0.0 9.879422743133691E-5 1.6465704571889486E-4 52-53 1.9307150045599024 1.7837846619546944E-4 0.0 1.0016636947899436E-4 1.6740132981420976E-4 54-55 2.360821162250418 1.8112275029078434E-4 0.0 1.0153851152665182E-4 1.7426204005249708E-4 56-57 2.8465251435699668 1.8386703438609927E-4 0.0 1.0153851152665182E-4 1.8386703438609927E-4 58-59 3.3926747263729213 1.9621631281501638E-4 0.0 1.0702707971728166E-4 1.9072774462438653E-4 60-61 3.9862674926156805 2.0033273895798872E-4 0.0 1.1114350586025404E-4 1.92099886672044E-4 62-63 4.617422647413062 2.0582130714861857E-4 0.0 1.1251564790791149E-4 1.92099886672044E-4 64-65 5.283242226760414 2.1405415943456332E-4 0.0 1.1388778995556894E-4 1.9758845486267382E-4 66-67 5.99512873107377 2.1954272762519315E-4 0.0 1.1663207405088386E-4 2.0582130714861857E-4 68-69 6.7500017014561395 2.2503129581582298E-4 0.0 1.1800421609854131E-4 2.113098753392484E-4 70-71 7.538241050011394 2.3463629014942516E-4 0.0 1.2074850019385623E-4 2.2228701172050805E-4 72-73 8.355325568692503 2.40124858340055E-4 1.3721420476574571E-6 1.2074850019385623E-4 2.2914772195879534E-4 74-75 9.202575258149317 2.469855685783423E-4 2.7442840953149143E-6 1.221206422415137E-4 2.3326414810176773E-4 76-77 10.078534275669266 2.6756769929320415E-4 2.7442840953149143E-6 1.2349278428917115E-4 2.3600843219708263E-4 78-79 10.97941962562696 2.8952197205572345E-4 2.7442840953149143E-6 1.2486492633682862E-4 2.373805742447401E-4 80-81 11.897495171157706 2.977548243416682E-4 2.7442840953149143E-6 1.2760921043214352E-4 2.469855685783423E-4 82-83 12.83672365849514 3.0324339253229803E-4 2.7442840953149143E-6 1.3309777862277334E-4 2.5384627881662957E-4 84-85 13.791608286596347 3.073598186752704E-4 2.7442840953149143E-6 1.344699206704308E-4 2.5796270495960196E-4 86-87 14.761612547920688 3.128483868659002E-4 2.7442840953149143E-6 1.399584888610606E-4 2.5796270495960196E-4 88 15.500443805623894 3.2108123915184495E-4 2.7442840953149143E-6 1.4270277295637553E-4 2.5796270495960196E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 217810 0.0 84.00618 8 AGTGGTA 218685 0.0 82.99641 5 TGGTATC 221275 0.0 82.63989 7 GTGGTAT 219675 0.0 82.58806 6 GTATCAA 221805 0.0 82.3872 9 AAGCAGT 215995 0.0 81.65301 1 GCAGTGG 224240 0.0 80.85558 3 CAGTGGT 233225 0.0 77.66991 4 AGCAGTG 233010 0.0 77.52028 2 CAACGCA 213295 0.0 44.46893 12-13 ATCAACG 214885 0.0 44.21091 10-11 ACGCAGA 216990 0.0 43.845955 14-15 AGAGTAC 219325 0.0 43.13551 18-19 TCAACGC 212730 0.0 42.891327 12-13 AACGCAG 215430 0.0 42.47048 14-15 GAGTACT 214340 0.0 42.2001 20-21 CGCAGAG 218530 0.0 41.839123 16-17 AGTACTT 227330 0.0 41.38386 20-21 TATCAAC 221380 0.0 41.27271 10-11 GCAGAGT 240290 0.0 39.69607 16-17 >>END_MODULE