FastQCFastQC Report
Thu 26 May 2016
SRR1660743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27531115
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT430607215.640746842254662No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT945460.3434150778128674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA664280.24128336247914406No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA351930.12782991171988495No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA302690.1099446934858977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7098250.090.733871
AGCAGTG7335350.087.830562
GCAGTGG7448800.086.419053
AGTGGTA7614350.084.362745
CAGTGGT7597450.084.186654
GTGGTAT7666900.083.73666
TGGTATC7664550.083.267777
GGTATCA7715050.082.9316948
GTATCAA7786000.082.04739
AGTACTT6596700.044.65614720-21
GAGTACT6580200.043.70061520-21
TACTTTT6602550.043.43480722-23
AGAGTAC7366500.043.17388518-19
GTACTTT6481050.043.1481522-23
ACGCAGA7652300.043.04853414-15
ATCAACG7752300.042.57451210-11
CAACGCA7743850.042.47714212-13
GCAGAGT7680000.042.45638316-17
AACGCAG7657750.041.81159614-15
CGCAGAG7655800.041.69926516-17
Produced by FastQC (version 0.11.5)