##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660743_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27531115 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85989328801249 34.0 31.0 34.0 30.0 34.0 2 31.96147148417345 34.0 31.0 34.0 30.0 34.0 3 32.035076094811274 34.0 31.0 34.0 30.0 34.0 4 35.4135435488174 37.0 35.0 37.0 33.0 37.0 5 35.38314924767849 37.0 35.0 37.0 33.0 37.0 6 35.47891031656364 37.0 35.0 37.0 33.0 37.0 7 35.20934132162827 37.0 35.0 37.0 33.0 37.0 8 35.374661614685785 37.0 35.0 37.0 33.0 37.0 9 37.07276261785983 39.0 37.0 39.0 33.0 39.0 10-11 36.76802274444751 39.0 37.0 39.0 32.5 39.0 12-13 37.089373532455916 39.0 37.0 39.0 33.0 39.0 14-15 38.55045774571789 41.0 38.0 41.0 34.0 41.0 16-17 38.49029329179004 40.5 38.0 41.0 33.5 41.0 18-19 38.36837233072471 40.0 38.0 41.0 33.0 41.0 20-21 38.07328170326556 40.0 38.0 41.0 32.5 41.0 22-23 37.60709050468897 39.5 37.0 41.0 32.5 41.0 24-25 37.795553067865214 40.0 38.0 41.0 32.5 41.0 26-27 38.053326572498065 40.0 38.0 41.0 33.0 41.0 28-29 38.07991459844616 40.0 38.0 41.0 33.0 41.0 30-31 37.98339555081587 40.0 38.0 41.0 32.5 41.0 32-33 37.82688381854494 40.0 38.0 41.0 32.0 41.0 34-35 37.6369652119066 40.0 37.5 41.0 31.5 41.0 36-37 37.270671147899385 39.5 37.0 40.5 31.0 41.0 38-39 36.134935599230175 38.0 35.0 40.0 30.0 41.0 40-41 35.94917096528782 38.0 35.0 40.0 30.0 41.0 42-43 35.71607472127445 38.0 35.0 40.0 28.5 41.0 44-45 35.52210482575805 38.0 35.0 40.0 27.0 41.0 46-47 35.399490013390306 38.0 35.0 41.0 25.5 41.0 48-49 35.073673223914106 38.0 35.0 41.0 24.5 41.0 50-51 33.4473408541572 36.5 32.0 39.0 21.5 40.0 52-53 33.18372670340449 36.5 33.0 39.5 19.0 40.0 54-55 32.62000373032476 37.0 31.0 40.0 11.0 41.0 56-57 31.538095805418706 36.5 27.5 40.0 7.5 41.0 58-59 31.187850818973367 36.0 26.0 40.0 7.0 41.0 60-61 30.953292719891657 35.5 26.0 40.0 7.0 41.0 62-63 30.705154658647132 35.0 26.0 40.0 7.0 41.0 64-65 30.46263872712747 35.0 25.5 39.5 7.0 41.0 66-67 30.24206271703852 35.0 25.0 39.0 7.0 41.0 68-69 30.020214255761164 35.0 25.0 39.0 7.0 41.0 70-71 29.765388379656983 35.0 25.0 37.5 7.0 40.5 72-73 29.421154446523506 35.0 24.5 37.0 7.0 40.0 74-75 28.99224068839929 35.0 24.0 36.5 7.0 39.0 76-77 28.54430498001988 35.0 22.5 36.0 7.0 39.0 78-79 28.145021950618418 34.0 20.0 35.5 7.0 38.5 80-81 27.805711610299838 34.0 20.0 35.0 7.0 37.0 82-83 27.49141758334161 34.0 19.0 35.0 7.0 37.0 84-85 27.208272984948124 34.0 19.0 35.0 7.0 36.5 86-87 26.984500863840786 34.0 18.5 35.0 7.0 36.0 88-89 26.769211054474184 34.0 18.0 35.0 7.0 36.0 90-91 26.57031455137215 34.0 17.0 35.0 7.0 35.5 92-93 26.388774174238854 33.5 17.0 35.0 7.0 35.0 94-95 26.192642397520043 33.0 15.0 35.0 7.0 35.0 96-97 26.029146240535482 33.0 15.0 35.0 7.0 35.0 98-99 25.8769004451872 33.0 15.0 35.0 7.0 35.0 100 25.752660980131026 33.0 13.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5133451590074145 1101 2 0.5528126471302244 1101 3 0.5567550217471222 1101 4 0.507875217883182 1101 5 0.5126043061315855 1101 6 0.4776349586508317 1101 7 0.5644038567818583 1101 8 0.5287138901313853 1101 9 0.5952132410565625 1101 10-11 0.6799875197110552 1101 12-13 0.6068144195950183 1101 14-15 0.6832455115022569 1101 16-17 0.6602842048472652 1101 18-19 0.6717342838069058 1101 20-21 0.6989835434070102 1101 22-23 0.8125325759949007 1101 24-25 0.7948730547693614 1101 26-27 0.7133162326130815 1101 28-29 0.6800862451680345 1101 30-31 0.6676015369437565 1101 32-33 0.678706574584055 1101 34-35 0.690468559316237 1101 36-37 0.656070796660245 1101 38-39 0.6673049965299143 1101 40-41 0.6816482764086658 1101 42-43 0.7426160322606634 1101 44-45 0.7603685955128157 1101 46-47 0.7498076783657055 1101 48-49 0.814148938325161 1101 50-51 0.7540926966317372 1101 52-53 0.8257308050544268 1101 54-55 0.8924193107107712 1101 56-57 0.8497892670996059 1101 58-59 0.6862392810281754 1101 60-61 0.7268677461920561 1101 62-63 0.6918990251325674 1101 64-65 0.6406900133829048 1101 66-67 0.6406976444138301 1101 68-69 0.6469071754566933 1101 70-71 0.5703053694015132 1101 72-73 0.5411697910725337 1101 74-75 0.4982529816840682 1101 76-77 0.532403959394145 1101 78-79 0.56654649359972 1101 80-81 0.48107403708468155 1101 82-83 0.4676996414374379 1101 84-85 0.44159314217007406 1101 86-87 0.42890638861076624 1101 88-89 0.378869419407291 1101 90-91 0.38509570320742625 1101 92-93 0.3800862532389431 1101 94-95 0.46677580520503525 1101 96-97 0.39756487059155177 1101 98-99 0.3812501786753302 1101 100 0.4136930699166932 1102 1 0.4925547597638271 1102 2 0.5179209930592421 1102 3 0.5068526225016896 1102 4 0.4461856257993233 1102 5 0.44599097981362945 1102 6 0.3913160618512279 1102 7 0.4483086741230977 1102 8 0.4779052343334911 1102 9 0.534754274687792 1102 10-11 0.5475728142084648 1102 12-13 0.5399216750102411 1102 14-15 0.5961013698836908 1102 16-17 0.5993225905646185 1102 18-19 0.564451036082815 1102 20-21 0.5743934642745572 1102 22-23 0.6540516389263829 1102 24-25 0.5998998446286379 1102 26-27 0.5412382422334474 1102 28-29 0.565597130627026 1102 30-31 0.5384786041843554 1102 32-33 0.5199992661392017 1102 34-35 0.543707250644438 1102 36-37 0.5469669686069736 1102 38-39 0.5720337996967757 1102 40-41 0.5496814974913491 1102 42-43 0.6007544115104295 1102 44-45 0.5919044166354155 1102 46-47 0.6371381371893747 1102 48-49 0.639178732966549 1102 50-51 0.6314308221954903 1102 52-53 0.634534022553467 1102 54-55 0.6751015522989405 1102 56-57 0.6090367228167644 1102 58-59 0.4596057429185372 1102 60-61 0.4465076453252834 1102 62-63 0.38172977108497363 1102 64-65 0.41177640265214066 1102 66-67 0.40557690879346 1102 68-69 0.36975056243879223 1102 70-71 0.3922552911574968 1102 72-73 0.3585007723669307 1102 74-75 0.30721922961461345 1102 76-77 0.3333346362745395 1102 78-79 0.3459106375153205 1102 80-81 0.2683341982772589 1102 82-83 0.3523199732362734 1102 84-85 0.2943532836836873 1102 86-87 0.33676256461757603 1102 88-89 0.28387648427030854 1102 90-91 0.24984272725327727 1102 92-93 0.2369480949459657 1102 94-95 0.25083753063354663 1102 96-97 0.23754843347826693 1102 98-99 0.1960944064440291 1102 100 0.2519465009037205 1103 1 0.37156973698487405 1103 2 0.39369405252003986 1103 3 0.39805494051208967 1103 4 0.3286985670916138 1103 5 0.35807016915389056 1103 6 0.33776850257970636 1103 7 0.3325995732965268 1103 8 0.3666614221968416 1103 9 0.4152139911437658 1103 10-11 0.448195834237076 1103 12-13 0.40668974282717585 1103 14-15 0.4831239239663887 1103 16-17 0.484534175765873 1103 18-19 0.46163375933866035 1103 20-21 0.4981883052003866 1103 22-23 0.5668878866216431 1103 24-25 0.5432568351300731 1103 26-27 0.4712753799665137 1103 28-29 0.46102448106355354 1103 30-31 0.4526139839135297 1103 32-33 0.39441003516601114 1103 34-35 0.43645488448047587 1103 36-37 0.39076892614355074 1103 38-39 0.43974572706341775 1103 40-41 0.44769312310138787 1103 42-43 0.49481110071314305 1103 44-45 0.5110475182485459 1103 46-47 0.5764552240880718 1103 48-49 0.6257799460599927 1103 50-51 0.5912734787038687 1103 52-53 0.5935487980167764 1103 54-55 0.6205588605401928 1103 56-57 0.5466074265627832 1103 58-59 0.4870037087712511 1103 60-61 0.5346096855896896 1103 62-63 0.4798087100882462 1103 64-65 0.42421179098820616 1103 66-67 0.39415427634003564 1103 68-69 0.3904695654922712 1103 70-71 0.39497884356181245 1103 72-73 0.3897394650798063 1103 74-75 0.41111044777990813 1103 76-77 0.39603849833978444 1103 78-79 0.33010530468623855 1103 80-81 0.2637891401577974 1103 82-83 0.3267869393057019 1103 84-85 0.34961080776279374 1103 86-87 0.2937204628571273 1103 88-89 0.30863194780906866 1103 90-91 0.3172711367032939 1103 92-93 0.2689356773294804 1103 94-95 0.34055913464477783 1103 96-97 0.33792051374150134 1103 98-99 0.33215055168784957 1103 100 0.2911942872385538 1104 1 0.41595972653842495 1104 2 0.4449733167019687 1104 3 0.4244791403809174 1104 4 0.36802782906897136 1104 5 0.38578151711782027 1104 6 0.31793816919846307 1104 7 0.3361231322224256 1104 8 0.36806354746229175 1104 9 0.4220207150388475 1104 10-11 0.39293924721053486 1104 12-13 0.41292288287679213 1104 14-15 0.4632728232903389 1104 16-17 0.46631252711767956 1104 18-19 0.4383096835645901 1104 20-21 0.393897448637901 1104 22-23 0.47648863587971846 1104 24-25 0.4824596634600553 1104 26-27 0.4064169620151503 1104 28-29 0.38547860049478544 1104 30-31 0.37193917656156117 1104 32-33 0.3509935280216254 1104 34-35 0.3260857677019189 1104 36-37 0.31434873362618987 1104 38-39 0.32607955921245235 1104 40-41 0.32154250597750433 1104 42-43 0.3568050683118358 1104 44-45 0.3418576458826408 1104 46-47 0.3942550215218219 1104 48-49 0.4163738979968983 1104 50-51 0.4040713155871529 1104 52-53 0.43372299608871856 1104 54-55 0.44625348207123494 1104 56-57 0.4668770391901056 1104 58-59 0.42377430099040225 1104 60-61 0.4031890477979623 1104 62-63 0.3620601339352163 1104 64-65 0.3286773921117856 1104 66-67 0.34389878382357963 1104 68-69 0.33198037965011906 1104 70-71 0.2865468882601512 1104 72-73 0.2773696654136799 1104 74-75 0.20679585699788916 1104 76-77 0.22738900571336984 1104 78-79 0.28845393512055395 1104 80-81 0.23010783415664804 1104 82-83 0.19985630848023916 1104 84-85 0.210943786243444 1104 86-87 0.18552676881408559 1104 88-89 0.15951860584078403 1104 90-91 0.1498472620650304 1104 92-93 0.1749239482912941 1104 94-95 0.25787741127108177 1104 96-97 0.22394454498343208 1104 98-99 0.20192468435076805 1104 100 0.1466818643193868 1105 1 0.29465703492859063 1105 2 0.35938681768784875 1105 3 0.3255651894627789 1105 4 0.31266652289325947 1105 5 0.30519709863955313 1105 6 0.28969737057422407 1105 7 0.3454210107647242 1105 8 0.32856936280599314 1105 9 0.3358269972205363 1105 10-11 0.3709056005430611 1105 12-13 0.37152240732856256 1105 14-15 0.38675139388863755 1105 16-17 0.3408656375156127 1105 18-19 0.37247973011681523 1105 20-21 0.4166793645169804 1105 22-23 0.499866181052937 1105 24-25 0.4999310545568605 1105 26-27 0.45565437907633566 1105 28-29 0.4338377486736036 1105 30-31 0.39046442961696215 1105 32-33 0.4055790993055908 1105 34-35 0.43794795639082196 1105 36-37 0.4238179143497973 1105 38-39 0.45160467918375247 1105 40-41 0.48174629624230647 1105 42-43 0.5233467395480815 1105 44-45 0.5532610730151006 1105 46-47 0.5558189417235226 1105 48-49 0.5741366000634471 1105 50-51 0.485716004839567 1105 52-53 0.5900379998943208 1105 54-55 0.6693157281063336 1105 56-57 0.6522026335351718 1105 58-59 0.6002072520350659 1105 60-61 0.5040083955735604 1105 62-63 0.5093493750598874 1105 64-65 0.5518796604146452 1105 66-67 0.4740409613261356 1105 68-69 0.5051924609224265 1105 70-71 0.4817823277453108 1105 72-73 0.4810703879568159 1105 74-75 0.46143237742437293 1105 76-77 0.41354322179479297 1105 78-79 0.4265665970066088 1105 80-81 0.4184678779697961 1105 82-83 0.4066614057509774 1105 84-85 0.4636682092593567 1105 86-87 0.4029500523863554 1105 88-89 0.4102183536840087 1105 90-91 0.41817688508471207 1105 92-93 0.345963111711427 1105 94-95 0.39673945755911433 1105 96-97 0.35680795168137536 1105 98-99 0.35484987880644425 1105 100 0.3292308581496961 1106 1 0.2355872122905538 1106 2 0.2504586505129325 1106 3 0.23610328928942437 1106 4 0.2448451743227622 1106 5 0.21531620723668965 1106 6 0.19868162398407918 1106 7 0.23200999100337327 1106 8 0.24514609722328373 1106 9 0.24991919293739784 1106 10-11 0.25125733114485627 1106 12-13 0.25299851439862664 1106 14-15 0.3089283909962077 1106 16-17 0.2687014885807102 1106 18-19 0.26897094666390586 1106 20-21 0.28042136790625705 1106 22-23 0.30783723072694613 1106 24-25 0.28553587654011636 1106 26-27 0.287998186728025 1106 28-29 0.263714295696424 1106 30-31 0.23066578362877266 1106 32-33 0.25051610427062343 1106 34-35 0.27725510328574643 1106 36-37 0.21853857643191077 1106 38-39 0.28400452041424273 1106 40-41 0.3083941244359778 1106 42-43 0.34250038598701593 1106 44-45 0.3623560790750986 1106 46-47 0.39580925908959586 1106 48-49 0.381843045308365 1106 50-51 0.2742962127013229 1106 52-53 0.36528035699213746 1106 54-55 0.5243965553422143 1106 56-57 0.5051061991283312 1106 58-59 0.42234034441854007 1106 60-61 0.3950913802703262 1106 62-63 0.3897693785126428 1106 64-65 0.3620125468352775 1106 66-67 0.3372956918832273 1106 68-69 0.3542925512615547 1106 70-71 0.3504008533244054 1106 72-73 0.2937382827544397 1106 74-75 0.33850990508025447 1106 76-77 0.33898504803317664 1106 78-79 0.33956168951356247 1106 80-81 0.2680553264703178 1106 82-83 0.28183094742063375 1106 84-85 0.2802505379181568 1106 86-87 0.21335624827436916 1106 88-89 0.22228696975385276 1106 90-91 0.22073807436027693 1106 92-93 0.2043196894879955 1106 94-95 0.26247722954420993 1106 96-97 0.2720188403575108 1106 98-99 0.24270316156484384 1106 100 0.23618041096358056 1107 1 0.19234942877213967 1107 2 0.21420125675665957 1107 3 0.23651131121678048 1107 4 0.22151460786313493 1107 5 0.239883131738587 1107 6 0.21120152585246643 1107 7 0.20809853127139633 1107 8 0.22309022257844902 1107 9 0.25721679866128255 1107 10-11 0.2517719422070641 1107 12-13 0.24417723782623568 1107 14-15 0.2418676507902191 1107 16-17 0.263100300106764 1107 18-19 0.23604143383203535 1107 20-21 0.2613456003097667 1107 22-23 0.3096280916431553 1107 24-25 0.30976988384124127 1107 26-27 0.2757313836166304 1107 28-29 0.28880020360191594 1107 30-31 0.24846769168468796 1107 32-33 0.20953189473164002 1107 34-35 0.23597290338791765 1107 36-37 0.2305970645134039 1107 38-39 0.2390434752176489 1107 40-41 0.2719214495350144 1107 42-43 0.2872412294479929 1107 44-45 0.2902306101920118 1107 46-47 0.2791502779303485 1107 48-49 0.32276862082087376 1107 50-51 0.19403633517659102 1107 52-53 0.1981007217360755 1107 54-55 0.263012814984684 1107 56-57 0.26813226164210846 1107 58-59 0.19624431231698125 1107 60-61 0.22640375970151183 1107 62-63 0.16321379926712254 1107 64-65 0.1401227829288807 1107 66-67 0.1515211380252275 1107 68-69 0.1813968395507537 1107 70-71 0.19652342971659564 1107 72-73 0.16813305826256197 1107 74-75 0.16913126681634694 1107 76-77 0.14907884253919335 1107 78-79 0.14084677895279896 1107 80-81 0.08413337270937049 1107 82-83 0.07136690353794606 1107 84-85 0.07765695636237169 1107 86-87 0.06904566737481588 1107 88-89 0.09615793002516426 1107 90-91 0.10443312313017827 1107 92-93 0.11947422471889624 1107 94-95 0.08693404805766036 1107 96-97 0.14684544943853695 1107 98-99 0.16676683735701303 1107 100 0.15614739200277583 1108 1 0.11068355430353449 1108 2 0.13469309395139817 1108 3 0.16174565800461949 1108 4 0.1550448216233633 1108 5 0.16407099216031185 1108 6 0.12470277224663562 1108 7 0.12314672976552288 1108 8 0.13215910102840667 1108 9 0.17292692860397096 1108 10-11 0.13319803668797192 1108 12-13 0.14800610834171124 1108 14-15 0.18569325947132853 1108 16-17 0.15507109833584565 1108 18-19 0.15098413035682512 1108 20-21 0.12031919164007121 1108 22-23 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2316 94-95 -1.2158833532305167 2316 96-97 -1.4172153916482841 2316 98-99 -1.2846964588686554 2316 100 -1.069122735817139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14345.0 3 3621.0 4 2516.0 5 2047.0 6 3244.0 7 12565.0 8 22303.0 9 25044.0 10 27478.0 11 33635.0 12 45962.0 13 60152.0 14 76389.0 15 96228.0 16 125773.0 17 163044.0 18 183395.0 19 207698.0 20 250487.0 21 334075.0 22 487110.0 23 790349.0 24 1151537.0 25 1295783.0 26 1162377.0 27 885680.0 28 679438.0 29 589255.0 30 563528.0 31 595373.0 32 679599.0 33 818449.0 34 1075225.0 35 1650443.0 36 2722429.0 37 4375723.0 38 4930098.0 39 1385765.0 40 2953.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.74412415665959 46.70404444165489 20.590308836926294 18.961522564759235 2 17.293010434119918 46.86916237153343 21.435594879751463 14.402232314595187 3 41.5617659837154 20.41061050834891 20.684299424304378 17.343324083631316 4 15.360259956631687 22.818661663186543 19.947219232668882 41.87385914751289 5 18.004751718518772 46.48478091503544 20.069104978887523 15.441362387558263 6 42.74288128595444 21.819375090104838 20.523417986280247 14.91432563766047 7 17.114856158327047 21.200346891548453 45.656310174846475 16.028486775278022 8 41.92262208813617 21.826574535918873 20.145276778722266 16.105526597222685 9 40.93706555991219 20.56692433276222 22.105031832357945 16.390978274967647 10-11 16.044076506765805 34.51697268481908 32.89851304938217 16.540437759032947 12-13 16.027172819135522 22.23131081699296 32.35236886254342 29.3891475013281 14-15 16.38809057400232 47.023726524857715 19.796859927968974 16.79132297317099 16-17 29.30738825546046 21.791516311064267 19.937349758448605 28.96374567502667 18-19 16.73855576079532 34.738657622777794 20.010861033880932 28.51192558254595 20-21 29.064198273939347 34.73839279569944 20.35341192977678 15.84399700058443 22-23 28.753387241230477 22.281401797300767 32.78710801871335 16.17810294275541 24-25 16.435395446545805 34.17357514794773 20.842619330421478 28.548410075084984 26-27 16.703123549204758 22.613850139818034 44.61271942386201 16.070306887115198 28-29 18.035026191508862 22.394653589376375 43.46604269866947 16.104277520445294 30-31 17.542815250733 22.476595632505823 43.562376119723176 16.418212997038008 32-33 16.92270906547715 22.948716508277986 43.54346281313903 16.585111613105838 34-35 16.7274493278698 22.984630679043097 43.57163703509282 16.716282957994288 36-37 16.646784139015043 23.143800305491613 43.54051763412343 16.668897921369904 38-39 16.575644418013812 23.51571433399396 43.230273317987255 16.678367930004974 40-41 16.554558836822014 23.72367636357369 42.923613723439665 16.79815107616463 42-43 16.72537627505217 23.982126298967803 42.55539231379172 16.737105112188306 44-45 16.82075805902801 24.299609183304565 42.12861932339918 16.75101343426825 46-47 16.766631158191576 24.918393671838977 41.528374925145464 16.786600244823983 48-49 16.91879612476702 25.24144769073871 40.98048808472451 16.859268099769757 50-51 17.060928048761202 25.640549763333002 40.37677836563519 16.921743822270603 52-53 17.403744732881982 26.083719974377573 39.37657379052041 17.135961502220038 54-55 18.241530012799533 27.08011695096278 37.04525630210958 17.633096734128113 56-57 19.357795423017603 28.580748789063875 33.870451878590444 18.19100390932808 58-59 19.493726891462366 29.891765017989407 32.248175219904546 18.366332870643674 60-61 19.592739521322777 30.600343810319792 31.338601896694446 18.468314771662982 62-63 19.575384334556738 30.941978513227014 30.81873073827397 18.66390641394228 64-65 19.547256949163636 30.853354703603415 30.836656073958345 18.7627322732746 66-67 19.59291114908186 30.326246091081586 31.399399928642858 18.681442831193696 68-69 19.631048827198853 29.81162449618035 32.09229423889491 18.46503243772589 70-71 19.685182168577438 29.42688287755138 32.470016565722645 18.41791838814853 72-73 19.875415261854062 29.202123048274743 32.448240179936256 18.47422150993494 74-75 20.30092418183188 29.309973419845136 32.01267582770986 18.37642657061312 76-77 20.83571275426834 29.553446206371504 31.325755404934046 18.285085634426107 78-79 21.289103625675086 29.90705989760336 30.563204791443177 18.240631685278377 80-81 21.640361670030867 30.19488420441184 29.941949714721012 18.222804410836282 82-83 21.990254668262406 30.47980461046945 29.247493078099634 18.282447643168513 84-85 22.15470641219967 30.694074111260512 28.793131884970595 18.358087591569223 86-87 22.391678410763877 30.815350277299387 28.462474395237336 18.330496916699403 88-89 22.685395826994633 30.90511140326485 28.128481891934115 18.281010877806406 90-91 22.885477364736666 30.96894708078901 27.7673827583919 18.37819279608242 92-93 23.157672907575677 30.935418311720113 27.484603609230895 18.42230517147331 94-95 23.46850176368519 30.86210394144745 27.297034810243698 18.37235948462366 96-97 23.774430569915896 30.821119847932664 27.0496436130998 18.35480596905164 98-99 24.22236230826193 30.81031655794017 26.714341905661875 18.252979228136024 100 24.54007644351845 30.71271072870886 26.503004140887565 18.244208686885123 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 65942.0 1 47131.0 2 24088.0 3 19067.5 4 18242.0 5 18386.5 6 19218.0 7 19694.0 8 19723.0 9 20310.5 10 22476.5 11 31890.5 12 266977.5 13 340148.0 14 185603.0 15 199032.0 16 226494.0 17 251192.0 18 271354.5 19 291339.5 20 313431.0 21 339384.5 22 370796.5 23 399696.0 24 422202.5 25 448683.0 26 480042.0 27 518870.0 28 566683.0 29 601684.0 30 626523.5 31 659717.5 32 689555.5 33 708559.5 34 716362.0 35 709978.5 36 704280.5 37 697109.5 38 687311.0 39 676005.0 40 663975.0 41 657840.5 42 654264.0 43 639217.5 44 621185.5 45 617037.5 46 612296.5 47 609032.0 48 607089.0 49 604042.0 50 604893.5 51 608448.0 52 612763.0 53 608197.5 54 594998.5 55 581199.5 56 565394.0 57 539080.5 58 498570.5 59 446131.5 60 380521.5 61 319351.5 62 266859.0 63 213097.5 64 165129.0 65 126027.0 66 95237.0 67 73541.5 68 58523.5 69 46117.5 70 35936.0 71 28909.0 72 24086.5 73 19648.0 74 16100.5 75 13501.5 76 10469.0 77 7137.0 78 4953.5 79 3710.5 80 2789.5 81 2150.0 82 1639.5 83 1276.5 84 1032.5 85 868.5 86 741.0 87 619.0 88 534.0 89 471.5 90 391.0 91 336.0 92 308.0 93 263.0 94 215.0 95 190.0 96 158.0 97 129.0 98 111.0 99 85.0 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4047565817802875 2 0.38743436290175676 3 0.1693574706291409 4 0.17571754721884675 5 0.2752703622791885 6 0.10326497855244876 7 0.1372846686376487 8 0.142805694575029 9 0.1358208703134617 10-11 0.1322957678975225 12-13 0.13420814957912167 14-15 0.14041022312390908 16-17 0.14442023143632213 18-19 0.15596898273099363 20-21 0.13715027524312037 22-23 0.10002500806814399 24-25 0.09522135227723251 26-27 0.08953142653321523 28-29 0.09935667334940847 30-31 0.10352831696064616 32-33 0.10473240913054192 34-35 0.10494126373014678 36-37 0.10135804525170883 38-39 0.10052081072633637 40-41 0.10163409654857784 42-43 0.0644034940103225 44-45 0.0675617387817384 46-47 0.08571211155087616 48-49 0.08987285840039534 50-51 0.0990878865603518 52-53 0.10404954539618173 54-55 0.10654127157581521 56-57 0.1069989355679928 58-59 0.10620528808949438 60-61 0.104394609517268 62-63 0.10900393972419933 64-65 0.11569636754631986 66-67 0.12429028028832106 68-69 0.12952980654797308 70-71 0.12778995692691705 72-73 0.11477559118110545 74-75 0.11223301344678556 76-77 0.114150843509244 78-79 0.11293222232372355 80-81 0.10725137721447171 82-83 0.11026796408354692 84-85 0.11638286353458623 86-87 0.12982401911437294 88-89 0.13795300335638422 90-91 0.14474895041483063 92-93 0.14386268046172487 94-95 0.13380315326858358 96-97 0.13049416996006155 98-99 0.12539085322189092 100 0.11951568252865895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7531115E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.377414901978135 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84926242112736 24.357089223673942 2 16.63894237354519 11.772855357580262 3 6.063964000072012 6.435821111436195 4 2.5702281570619725 3.6371211162051207 5 1.430773152395887 2.5308527721460248 6 0.8895712579979549 1.8882438887440969 7 0.5760169280043768 1.4264592896801591 8 0.4479188456716625 1.2676968676593214 9 0.336241803525713 1.0705829211646705 >10 1.8716013416985695 12.723813124839978 >50 0.219466077543437 5.346859624890408 >100 0.09847739126454674 6.097733974571589 >500 0.0038518811062940375 0.9333005283861684 >1k 0.0030489139686168307 2.094957980732747 >5k 4.0997097827106914E-4 1.0090052113218542 >10k+ 2.2548403804848707E-4 17.40760700696747 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4306072 15.640746842254662 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 94546 0.3434150778128674 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66428 0.24128336247914406 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 35193 0.12782991171988495 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA 30269 0.1099446934858977 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016926303202758042 3.6322539061712538E-6 0.0 0.0 7.264507812342508E-5 2 0.0016962625741819757 3.6322539061712538E-6 0.0 0.0 7.264507812342508E-5 3 0.0016962625741819757 1.0896761718513762E-5 0.0 0.0 7.264507812342508E-5 4 0.0016962625741819757 1.4529015624685015E-5 0.0 3.6322539061712538E-6 7.627733202959634E-5 5 0.0016962625741819757 2.905803124937003E-5 0.0 1.0896761718513762E-5 7.627733202959634E-5 6 0.0016962625741819757 3.995479296788379E-5 0.0 1.0896761718513762E-5 8.354183984193884E-5 7 0.0016998948280881469 3.995479296788379E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.354183984193884E-5 8 0.0016998948280881469 3.995479296788379E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.354183984193884E-5 9 0.0017071593359004893 4.358704687405505E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.354183984193884E-5 10-11 0.0017107915898066607 6.174831640491131E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.71740937481101E-5 12-13 0.0017471141288683731 6.538057031108257E-5 3.6322539061712538E-6 1.4529015624685015E-5 8.899022070119572E-5 14-15 0.0017997818105078562 7.627733202959634E-5 3.6322539061712538E-6 1.4529015624685015E-5 9.080634765428135E-5 16-17 0.0018978526659744802 8.71740937481101E-5 3.6322539061712538E-6 2.905803124937003E-5 9.080634765428135E-5 18-19 0.0019686816171448195 8.71740937481101E-5 3.6322539061712538E-6 3.2690285155541284E-5 9.080634765428135E-5 20-21 0.0021030750116731558 9.080634765428135E-5 3.6322539061712538E-6 3.2690285155541284E-5 9.988698241970948E-5 22-23 0.002326458626902688 9.44386015604526E-5 3.6322539061712538E-6 3.2690285155541284E-5 1.1441599804439449E-4 24-25 0.0028658483319691196 9.44386015604526E-5 3.6322539061712538E-6 3.2690285155541284E-5 1.1623212499748012E-4 26-27 0.004117159802645117 9.625472851353822E-5 3.6322539061712538E-6 3.632253906171254E-5 1.2349663280982262E-4 28-29 0.0071137692752364 1.0715149023205199E-4 3.6322539061712538E-6 3.632253906171254E-5 1.289450136690795E-4 30-31 0.013186897806354738 1.1986437890365138E-4 3.6322539061712538E-6 4.358704687405505E-5 1.3620952148142202E-4 32-33 0.02433973342525357 1.2349663280982262E-4 3.6322539061712538E-6 4.540317382714067E-5 1.471062831999358E-4 34-35 0.04345991798733905 1.2712888671599388E-4 3.6322539061712538E-6 5.0851554686397554E-5 1.5255466405919267E-4 36-37 0.07354587709215554 1.2712888671599388E-4 3.6322539061712538E-6 5.448380859256881E-5 1.5800304491844953E-4 38-39 0.11764143951307457 1.416579023406789E-4 3.6322539061712538E-6 5.993218945182568E-5 1.6526755273079205E-4 40-41 0.17639677869930076 1.4892241015302142E-4 5.448380859256881E-6 6.174831640491131E-5 1.8524494921473393E-4 42-43 0.2502150748344192 1.6708367968387767E-4 7.2645078123425075E-6 6.174831640491131E-5 1.888772031209052E-4 44-45 0.3410141579808882 1.7798044140239144E-4 1.0896761718513762E-5 6.538057031108257E-5 1.9432558398016207E-4 46-47 0.45386465459172287 1.9250945702707644E-4 1.4529015624685015E-5 6.538057031108257E-5 1.9977396483941896E-4 48-49 0.5892659995790217 2.0340621874559021E-4 1.4529015624685015E-5 6.71966972641682E-5 2.0522234569867584E-4 50-51 0.7473108154173923 2.0340621874559021E-4 1.4529015624685015E-5 6.901282421725382E-5 2.1793523437027524E-4 52-53 0.9200317531636477 2.0703847265176147E-4 1.4529015624685015E-5 6.901282421725382E-5 2.215674882764465E-4 54-55 1.1139069376594446 2.0703847265176147E-4 1.4529015624685015E-5 6.901282421725382E-5 2.3246424999496024E-4 56-57 1.3300296773305402 2.3246424999496024E-4 1.4529015624685015E-5 6.901282421725382E-5 2.3791263085421713E-4 58-59 1.57452395226274 2.4154488476038838E-4 1.4529015624685015E-5 6.901282421725382E-5 2.43361011713474E-4 60-61 1.8413002887823469 2.43361011713474E-4 1.4529015624685015E-5 6.901282421725382E-5 2.57890027338159E-4 62-63 2.123924149094579 2.4880939257273084E-4 1.4529015624685015E-5 7.264507812342508E-5 2.6152228124433027E-4 64-65 2.4198075522912896 2.506255195258165E-4 1.4529015624685015E-5 7.990958593576758E-5 2.6152228124433027E-4 66-67 2.7393914122257668 2.5244164647890215E-4 1.4529015624685015E-5 7.990958593576758E-5 2.742351699159297E-4 68-69 3.0819474619898246 2.6152228124433027E-4 1.4529015624685015E-5 8.172571288885321E-5 2.778674238221009E-4 70-71 3.447933365575641 2.687867890566728E-4 1.4529015624685015E-5 8.71740937481101E-5 2.8694805858752907E-4 72-73 3.827750892036156 2.760512968690153E-4 1.4529015624685015E-5 8.71740937481101E-5 2.942125663998716E-4 74-75 4.221665195906523 2.9784482030604284E-4 1.4529015624685015E-5 9.080634765428135E-5 3.087415820245566E-4 76-77 4.633887875590945 3.014770742122141E-4 1.4529015624685015E-5 9.44386015604526E-5 3.123738359307278E-4 78-79 5.0610609123531685 3.123738359307278E-4 1.4529015624685015E-5 9.44386015604526E-5 3.196383437430703E-4 80-81 5.508926899618849 3.232705976492416E-4 1.4529015624685015E-5 9.44386015604526E-5 3.323512324146697E-4 82-83 5.961956135812153 3.486963749924404E-4 1.4529015624685015E-5 9.807085546662385E-5 3.35983486320841E-4 84-85 6.425954778802094 3.6504151757021106E-4 1.4529015624685015E-5 1.0896761718513762E-4 3.4143186718009786E-4 86-87 6.903719664096423 3.777544062418104E-4 1.4529015624685015E-5 1.1441599804439449E-4 3.468802480393547E-4 88 7.270003412502545 3.922834218664954E-4 1.4529015624685015E-5 1.1623212499748012E-4 3.5232862889861163E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 709825 0.0 90.73387 1 AGCAGTG 733535 0.0 87.83056 2 GCAGTGG 744880 0.0 86.41905 3 AGTGGTA 761435 0.0 84.36274 5 CAGTGGT 759745 0.0 84.18665 4 GTGGTAT 766690 0.0 83.7366 6 TGGTATC 766455 0.0 83.26777 7 GGTATCA 771505 0.0 82.931694 8 GTATCAA 778600 0.0 82.0473 9 AGTACTT 659670 0.0 44.656147 20-21 GAGTACT 658020 0.0 43.700615 20-21 TACTTTT 660255 0.0 43.434807 22-23 AGAGTAC 736650 0.0 43.173885 18-19 GTACTTT 648105 0.0 43.14815 22-23 ACGCAGA 765230 0.0 43.048534 14-15 ATCAACG 775230 0.0 42.574512 10-11 CAACGCA 774385 0.0 42.477142 12-13 GCAGAGT 768000 0.0 42.456383 16-17 AACGCAG 765775 0.0 41.811596 14-15 CGCAGAG 765580 0.0 41.699265 16-17 >>END_MODULE