FastQCFastQC Report
Thu 26 May 2016
SRR1660743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27531115
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT416468315.127186094715018No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1015170.3687355197927872No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT881820.3202994139539935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512870.1862874060858051No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA318880.11582531255998893No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA314800.11434335296627107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6544800.092.294621
AGCAGTG6926200.089.092562
GCAGTGG7071750.087.523283
AGTGGTA7444800.083.386055
CAGTGGT7417600.083.2725144
GTGGTAT7494600.082.830096
TGGTATC7579850.082.399787
GGTATCA7623800.081.984378
GTATCAA7698900.081.05669
TAGCAGT221450.057.6281431
AGTACTT6641650.044.3605920-21
GAGTACT6616150.043.2700620-21
TACTTTT6646050.043.1781222-23
GTACTTT6531050.042.7168422-23
AGAGTAC7373900.042.66494418-19
ACGCAGA7550150.042.5938514-15
ATCAACG7644950.042.1359410-11
CAACGCA7641700.042.01319512-13
GCAGAGT7657900.041.95718416-17
AACGCAG7543200.041.2634614-15
Produced by FastQC (version 0.11.5)