##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660743_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27531115 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.292706888188146 34.0 31.0 34.0 27.0 34.0 2 31.925276400901307 34.0 31.0 34.0 30.0 34.0 3 31.69781209369835 34.0 31.0 34.0 30.0 34.0 4 35.588767036859934 37.0 35.0 37.0 33.0 37.0 5 35.92305084628792 37.0 35.0 37.0 35.0 37.0 6 35.39280159920875 37.0 35.0 37.0 33.0 37.0 7 35.386106665131436 37.0 35.0 37.0 33.0 37.0 8 35.90442719083481 37.0 35.0 37.0 33.0 37.0 9 37.7456801876713 39.0 37.0 39.0 35.0 39.0 10-11 37.50996361752875 39.0 37.0 39.0 34.5 39.0 12-13 37.88916854620672 39.0 38.0 39.0 35.0 39.0 14-15 39.40951830319985 41.0 39.0 41.0 36.0 41.0 16-17 39.35185783430856 41.0 39.0 41.0 36.0 41.0 18-19 39.28069230033001 40.5 39.0 41.0 36.0 41.0 20-21 39.069555464789566 40.5 38.5 41.0 35.5 41.0 22-23 38.652627454427474 40.0 38.0 41.0 35.0 41.0 24-25 38.84263808421853 40.0 38.5 41.0 35.0 41.0 26-27 39.03536425967492 40.0 39.0 41.0 35.0 41.0 28-29 39.03337985039836 41.0 39.0 41.0 35.0 41.0 30-31 38.92630514601389 40.0 38.5 41.0 35.0 41.0 32-33 38.773433150092174 40.0 38.0 41.0 35.0 41.0 34-35 38.64096583084267 40.0 38.0 41.0 35.0 41.0 36-37 38.319638198452914 39.5 38.0 41.0 34.0 41.0 38-39 37.200394281161515 39.0 35.0 41.0 33.5 41.0 40-41 36.961704820164385 39.0 35.0 41.0 33.0 41.0 42-43 36.624959486748 38.0 35.0 41.0 32.5 41.0 44-45 36.343018671782815 38.0 35.0 40.5 31.5 41.0 46-47 36.03337358476037 38.0 35.0 40.0 30.5 41.0 48-49 35.85687125276256 38.0 35.0 40.0 30.0 41.0 50-51 35.564966075656585 38.0 35.0 41.0 28.0 41.0 52-53 34.83494088415961 38.0 34.5 40.0 23.0 41.0 54-55 33.46454313601174 37.0 33.0 40.0 15.0 41.0 56-57 32.20018068647056 37.0 30.5 40.0 9.0 41.0 58-59 31.85526919269343 37.0 30.0 40.0 7.0 41.0 60-61 31.691102176573672 36.0 29.0 40.0 7.0 41.0 62-63 31.43997302688249 35.5 29.0 40.0 7.0 41.0 64-65 31.184199586540537 35.0 29.0 39.5 7.0 41.0 66-67 30.946261584392786 35.0 29.0 39.0 7.0 41.0 68-69 30.72896945873787 35.0 28.5 39.0 7.0 41.0 70-71 30.473069743088864 35.0 28.0 38.0 7.0 40.5 72-73 30.129101164264505 35.0 26.5 37.0 7.0 40.0 74-75 29.70500426517415 35.0 26.0 37.0 7.0 39.0 76-77 28.013678087502086 33.5 23.0 35.0 7.0 38.0 78-79 28.84487894878213 35.0 25.0 36.0 7.0 38.5 80-81 28.73546719775062 35.0 25.0 35.0 7.0 37.0 82-83 28.480378764172826 35.0 24.0 35.0 7.0 37.0 84-85 28.236682386456195 35.0 24.0 35.0 7.0 36.5 86-87 28.021902055183745 35.0 23.0 35.0 7.0 36.0 88-89 27.841833449171965 34.5 21.0 35.0 7.0 36.0 90-91 27.66058772047554 34.0 20.0 35.0 7.0 36.0 92-93 27.485161679793936 34.0 20.0 35.0 7.0 35.0 94-95 27.350716344034737 34.0 19.0 35.0 7.0 35.0 96-97 27.216021436109653 34.0 19.0 35.0 7.0 35.0 98-99 27.06838932967299 34.0 19.0 35.0 7.0 35.0 100 26.96625904907956 34.0 19.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.4911663731909464 1101 2 1.10775399032147 1101 3 1.4825836160964911 1101 4 0.9226814902096336 1101 5 0.5424838431892809 1101 6 1.0633869253208559 1101 7 0.7858307184525515 1101 8 0.445044541378941 1101 9 0.5043401567374417 1101 10-11 0.40479572432401767 1101 12-13 0.3528971856051015 1101 14-15 0.44391358607304454 1101 16-17 0.4979086984509351 1101 18-19 0.4647013736152559 1101 20-21 0.5008916398698275 1101 22-23 0.5292578349395924 1101 24-25 0.4782926354329007 1101 26-27 0.460346439553625 1101 28-29 0.423502922371938 1101 30-31 0.43961326323657346 1101 32-33 0.46378068743469214 1101 34-35 0.48377997125517425 1101 36-37 0.4585803556385244 1101 38-39 0.504410269173853 1101 40-41 0.5081574093742631 1101 42-43 0.5172100775588788 1101 44-45 0.49702191609833335 1101 46-47 0.49723810665332735 1101 48-49 0.5089885199948938 1101 50-51 0.49324503361590644 1101 52-53 0.4868347464653766 1101 54-55 0.5426267016498585 1101 56-57 0.5080483454304385 1101 58-59 0.4436739987524021 1101 60-61 0.4178875441628378 1101 62-63 0.3861328077526238 1101 64-65 0.38849519144845956 1101 66-67 0.4294878937821167 1101 68-69 0.4282391365058942 1101 70-71 0.4134588325672901 1101 72-73 0.3740744060399379 1101 74-75 0.37245207566313 1101 76-77 0.30399959259649023 1101 78-79 0.44211427792140867 1101 80-81 0.43718686952219343 1101 82-83 0.4105378003905358 1101 84-85 0.4130622907955015 1101 86-87 0.4073604385304641 1101 88-89 0.38482876589391424 1101 90-91 0.38858203876659303 1101 92-93 0.3716520424859411 1101 94-95 0.34810983264151574 1101 96-97 0.3885027667503529 1101 98-99 0.36189718182256314 1101 100 0.4265853483573352 1102 1 1.4348425900241217 1102 2 1.0620204356630936 1102 3 1.4536832282482877 1102 4 0.8804328613500587 1102 5 0.4776942274216154 1102 6 1.0538779925674433 1102 7 0.7929760580711829 1102 8 0.4328768770693756 1102 9 0.4711635876393103 1102 10-11 0.362439545900493 1102 12-13 0.3581366175988663 1102 14-15 0.4359869800912435 1102 16-17 0.454101702821049 1102 18-19 0.4072156282048809 1102 20-21 0.4079267657841186 1102 22-23 0.4377170330828122 1102 24-25 0.4419705278550268 1102 26-27 0.41407345112923366 1102 28-29 0.42425644830133535 1102 30-31 0.3792203997724428 1102 32-33 0.3927540428084626 1102 34-35 0.359882700969095 1102 36-37 0.3822557484870188 1102 38-39 0.4071483955662032 1102 40-41 0.39962798264894417 1102 42-43 0.4405865860780338 1102 44-45 0.38220943935921525 1102 46-47 0.38014323264357586 1102 48-49 0.3328331289140394 1102 50-51 0.36790106029275194 1102 52-53 0.4130684391066666 1102 54-55 0.4574491502146856 1102 56-57 0.4268773101489316 1102 58-59 0.4283155516090602 1102 60-61 0.4138050280818959 1102 62-63 0.3762506191480597 1102 64-65 0.33776412945880097 1102 66-67 0.3629173720870398 1102 68-69 0.2465679448284135 1102 70-71 0.2698555372270164 1102 72-73 0.2731759635320863 1102 74-75 0.2477994899278677 1102 76-77 0.09844255824162573 1102 78-79 0.29535332024867245 1102 80-81 0.32985315849085595 1102 82-83 0.30668971715611093 1102 84-85 0.31253162625186093 1102 86-87 0.31375709715752365 1102 88-89 0.3124324021060616 1102 90-91 0.308247002685043 1102 92-93 0.3216506154733736 1102 94-95 0.25597591717912493 1102 96-97 0.2613765583066261 1102 98-99 0.3277631275716857 1102 100 0.4057629449045166 1103 1 1.3781660687081647 1103 2 1.0023685099749002 1103 3 1.3585948630089675 1103 4 0.8372085301393994 1103 5 0.4593843477100137 1103 6 0.9949654231389928 1103 7 0.7255800049819996 1103 8 0.40463620598593053 1103 9 0.4024177073069737 1103 10-11 0.3183789020314549 1103 12-13 0.2691169695430631 1103 14-15 0.3170133710767047 1103 16-17 0.34268275852771524 1103 18-19 0.29773855743535194 1103 20-21 0.3296122957262142 1103 22-23 0.3378913943389179 1103 24-25 0.3339878612773646 1103 26-27 0.3424612837352683 1103 28-29 0.32616762173793745 1103 30-31 0.31345699248775105 1103 32-33 0.34072021211368053 1103 34-35 0.35489318199746833 1103 36-37 0.3278586317338963 1103 38-39 0.3331295159068972 1103 40-41 0.32691647883046215 1103 42-43 0.36175164723244535 1103 44-45 0.31106721188652386 1103 46-47 0.34867792198924263 1103 48-49 0.2951577114704804 1103 50-51 0.2842207429979453 1103 52-53 0.2890075873985367 1103 54-55 0.30825615970781683 1103 56-57 0.3390630858849093 1103 58-59 0.31114927160895434 1103 60-61 0.27854766104471906 1103 62-63 0.20792898096679124 1103 64-65 0.16895923677639146 1103 66-67 0.16680279150596533 1103 68-69 0.1753411472367148 1103 70-71 0.1130910169836703 1103 72-73 0.1491899947096691 1103 74-75 0.19667615721054332 1103 76-77 0.09165288624891232 1103 78-79 0.27501545330446575 1103 80-81 0.27042488519153807 1103 82-83 0.24903872921213832 1103 84-85 0.2407049803648107 1103 86-87 0.25818507247067757 1103 88-89 0.27230998166282205 1103 90-91 0.27013504266512456 1103 92-93 0.266926958827554 1103 94-95 0.26737394162743655 1103 96-97 0.3390891893212711 1103 98-99 0.32835630069077126 1103 100 0.3105214190694028 1104 1 1.3678068000459227 1104 2 0.970990288130892 1104 3 1.359459925410654 1104 4 0.839454555785835 1104 5 0.45571608345217385 1104 6 0.9940978392047768 1104 7 0.7488391395104941 1104 8 0.406885165565086 1104 9 0.4268290290257539 1104 10-11 0.31988594779427615 1104 12-13 0.2742102060363436 1104 14-15 0.30199375649932136 1104 16-17 0.35344025258720535 1104 18-19 0.33515830642221545 1104 20-21 0.32306265621228647 1104 22-23 0.3298205342788165 1104 24-25 0.3193002524110753 1104 26-27 0.31545478070287203 1104 28-29 0.2859649668752553 1104 30-31 0.30160187767329916 1104 32-33 0.3140079566101406 1104 34-35 0.3115296564934198 1104 36-37 0.30026908321759294 1104 38-39 0.3251929038884356 1104 40-41 0.3223366511048624 1104 42-43 0.3665465357607971 1104 44-45 0.32560851295159665 1104 46-47 0.34216499787424226 1104 48-49 0.25569550934918794 1104 50-51 0.23706659999391633 1104 52-53 0.2679013408814015 1104 54-55 0.2982140915901894 1104 56-57 0.29218284140653594 1104 58-59 0.25896132486909096 1104 60-61 0.2674728486119804 1104 62-63 0.23782118356575666 1104 64-65 0.15843973296968983 1104 66-67 0.19996741899513282 1104 68-69 0.15489260241903224 1104 70-71 0.15662479895655324 1104 72-73 0.1403767377358811 1104 74-75 0.13748771151359662 1104 76-77 0.028894345211401884 1104 78-79 0.12793725909598308 1104 80-81 0.19672324644754724 1104 82-83 0.21325074758558316 1104 84-85 0.20704965557504096 1104 86-87 0.17751815963645257 1104 88-89 0.19390973804978628 1104 90-91 0.18350517081388773 1104 92-93 0.15249143487395145 1104 94-95 0.16742143481052096 1104 96-97 0.22005929758998732 1104 98-99 0.19935357104932905 1104 100 0.21075707867203164 1105 1 1.2930244912057454 1105 2 0.9421444963777503 1105 3 1.2915132112393266 1105 4 0.816152314195314 1105 5 0.4174146960645402 1105 6 0.9795236185825047 1105 7 0.7073677599702677 1105 8 0.3350844569919431 1105 9 0.3396608700955568 1105 10-11 0.25768229934216436 1105 12-13 0.2329694379833782 1105 14-15 0.28611273980200025 1105 16-17 0.2903771193875855 1105 18-19 0.2635628611062515 1105 20-21 0.2792186268128418 1105 22-23 0.30397418819135424 1105 24-25 0.30062897294060775 1105 26-27 0.3047487225761003 1105 28-29 0.29701322846074163 1105 30-31 0.30572489881209464 1105 32-33 0.3428704950406214 1105 34-35 0.34918691556940473 1105 36-37 0.3268310709637845 1105 38-39 0.370613099597378 1105 40-41 0.40105817935948807 1105 42-43 0.3638249301298444 1105 44-45 0.3459858901733739 1105 46-47 0.3433475372736794 1105 48-49 0.3643989213127128 1105 50-51 0.36586374497858287 1105 52-53 0.3391881090314257 1105 54-55 0.368951823758529 1105 56-57 0.3497724249901708 1105 58-59 0.3060407003412138 1105 60-61 0.2162772748957309 1105 62-63 0.22956954651452577 1105 64-65 0.23547491222400296 1105 66-67 0.244305922393373 1105 68-69 0.2131789541230944 1105 70-71 0.2179567190942997 1105 72-73 0.2300112178110041 1105 74-75 0.2568350791460148 1105 76-77 0.16243880855024528 1105 78-79 0.27584732011247226 1105 80-81 0.28187669633671675 1105 82-83 0.3044232798689208 1105 84-85 0.2941557285164258 1105 86-87 0.2992150381042613 1105 88-89 0.28098835942736144 1105 90-91 0.3000352240721327 1105 92-93 0.3053685279221767 1105 94-95 0.28622322261032096 1105 96-97 0.2642737866430025 1105 98-99 0.2784797534234471 1105 100 0.26420835548007204 1106 1 1.2625278073490982 1106 2 0.9280570241068489 1106 3 1.275635507295334 1106 4 0.7565669660040726 1106 5 0.37083234711634816 1106 6 0.934253740859134 1106 7 0.654797629724797 1106 8 0.31546011215456105 1106 9 0.28690418977107157 1106 10-11 0.20538105603121437 1106 12-13 0.18234458696581157 1106 14-15 0.22271653939105107 1106 16-17 0.2376889037224572 1106 18-19 0.1989171777447467 1106 20-21 0.20786810285800073 1106 22-23 0.24358143238360697 1106 24-25 0.23011325238462632 1106 26-27 0.19242472842588398 1106 28-29 0.22085692169179083 1106 30-31 0.22080539861718051 1106 32-33 0.25150608307820477 1106 34-35 0.26253477289738214 1106 36-37 0.22720070879785936 1106 38-39 0.2559610685447211 1106 40-41 0.25089637905499984 1106 42-43 0.26406211150040804 1106 44-45 0.2163181764064177 1106 46-47 0.2284171834800759 1106 48-49 0.19373463647171718 1106 50-51 0.21211389796766156 1106 52-53 0.19574775350984197 1106 54-55 0.22932710253909505 1106 56-57 0.19813981744946574 1106 58-59 0.1171640233510729 1106 60-61 0.12389968866156664 1106 62-63 0.061090033745553995 1106 64-65 0.022989100776381832 1106 66-67 0.021991845583464453 1106 68-69 0.04734125200337047 1106 70-71 -0.0030854703769769287 1106 72-73 -0.027168480943416284 1106 74-75 0.04635621004519308 1106 76-77 -0.06525476750330128 1106 78-79 0.0660531092742005 1106 80-81 0.10989836131581399 1106 82-83 0.10776372906053666 1106 84-85 0.07115461370703713 1106 86-87 0.08255318145259238 1106 88-89 0.106422081286766 1106 90-91 0.146165057766531 1106 92-93 0.08601804405601854 1106 94-95 0.043626478796110035 1106 96-97 0.09298339907052977 1106 98-99 0.10714259012706862 1106 100 0.10110129463696182 1107 1 1.219117668645918 1107 2 0.8618966441670395 1107 3 1.2602723559410691 1107 4 0.7725477768054887 1107 5 0.35381888268086925 1107 6 0.9277795185698992 1107 7 0.6640226719509386 1107 8 0.30948203181343104 1107 9 0.29871850743852235 1107 10-11 0.20875926872238182 1107 12-13 0.14071241063521 1107 14-15 0.17330524812120274 1107 16-17 0.18815828180700578 1107 18-19 0.15913297655171732 1107 20-21 0.14665744891221522 1107 22-23 0.22132842140116793 1107 24-25 0.21528378680736893 1107 26-27 0.17893420363922985 1107 28-29 0.16256805961288734 1107 30-31 0.17108489255184622 1107 32-33 0.19070399384731473 1107 34-35 0.22680206830000316 1107 36-37 0.210548689656882 1107 38-39 0.23619510118958686 1107 40-41 0.2398597195806218 1107 42-43 0.27351769846648466 1107 44-45 0.2401389134684777 1107 46-47 0.2626598447945554 1107 48-49 0.2603058383032817 1107 50-51 0.22570265500932152 1107 52-53 0.22662526180616993 1107 54-55 0.17634089647051354 1107 56-57 0.12528340492197998 1107 58-59 0.14670454898843843 1107 60-61 0.1403805221712986 1107 62-63 0.03311572667222151 1107 64-65 0.06542088781293387 1107 66-67 0.10652405776398766 1107 68-69 0.08460527699841691 1107 70-71 0.10406207336808038 1107 72-73 0.08529131898250242 1107 74-75 0.06685135066976855 1107 76-77 -0.03387925471245268 1107 78-79 0.09574632002211914 1107 80-81 0.10690643720239734 1107 82-83 0.11916040756128865 1107 84-85 0.09842955303295042 1107 86-87 0.06801808969882828 1107 88-89 0.12240794649843423 1107 90-91 0.129249679477212 1107 92-93 0.15836668327878556 1107 94-95 0.11914440530019377 1107 96-97 0.13011034324332016 1107 98-99 0.1349348585493928 1107 100 0.10112084181587733 1108 1 1.173011387854121 1108 2 0.8166433604377872 1108 3 1.2000798733812346 1108 4 0.7044445973663613 1108 5 0.30680363278519707 1108 6 0.8550537249557877 1108 7 0.6011729755987787 1108 8 0.25978892145545274 1108 9 0.20724027402156509 1108 10-11 0.10795496497462409 1108 12-13 0.09959938920405165 1108 14-15 0.13222517431852054 1108 16-17 0.11773049434255256 1108 18-19 0.09467434533366514 1108 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-0.7978647758223367 2316 96-97 -0.8611503178587121 2316 98-99 -1.0115939702372074 2316 100 -0.9568728098197603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 61.0 7 893.0 8 1092.0 9 427.0 10 456.0 11 865.0 12 1161.0 13 2072.0 14 5371.0 15 14925.0 16 34801.0 17 60995.0 18 87822.0 19 124391.0 20 176339.0 21 255334.0 22 399171.0 23 675221.0 24 1089153.0 25 1317876.0 26 1217850.0 27 914119.0 28 678250.0 29 585582.0 30 564150.0 31 601971.0 32 671378.0 33 802462.0 34 1049739.0 35 1648492.0 36 2822718.0 37 4780983.0 38 5481890.0 39 1459908.0 40 3197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.318035002854575 45.31852253641942 21.460879130378313 18.902563330347686 2 17.6860007516334 46.160650632934754 21.845651579923093 14.307697035508756 3 40.94338511811787 20.703773993005683 21.03107533650831 17.32176555236814 4 15.696700016472404 23.14031563448991 20.319971435727027 40.843012913310666 5 18.264836998205812 45.92024774302582 20.313475941469555 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22.54117199891799 44.51239129247943 16.15992580360629 28-29 17.861575898903098 22.44844969585138 43.50697428998721 16.18300011525831 30-31 17.448814947128987 22.47304623393563 43.57119208566621 16.506946733269174 32-33 16.96038616630944 22.926098012860102 43.5173665855342 16.596149235296252 34-35 16.753913331306826 22.983370957420654 43.54376800681651 16.718947704456014 36-37 16.651277536905713 23.132423808188197 43.511014017635894 16.7052846372702 38-39 16.561058076963008 23.50937763738446 43.217354588192606 16.712209697459922 40-41 16.563719783257852 23.704598092317035 42.90560657649202 16.826075547933083 42-43 16.728003654298256 23.94873759358967 42.532735892067485 16.79052286004459 44-45 16.81360062281934 24.253109214245047 42.139989703852876 16.793300459082737 46-47 16.796358448006433 24.84328282733685 41.5388223392671 16.821536385389617 48-49 16.88382016712717 25.189049034854015 41.02070726778882 16.906423530229986 50-51 17.02902549086717 25.594517826768552 40.38869160239266 16.987765079971613 52-53 17.37253728729698 25.992482653118653 39.41773771919924 17.21724234038513 54-55 18.175934303721462 26.865159298113227 37.20191078817323 17.756995609992078 56-57 19.36034056266045 28.34699135287282 33.884558740383184 18.408109344083545 58-59 19.479927276615097 29.75755378095429 32.12243882907511 18.64008011335551 60-61 19.459527306410905 30.48544962600608 31.27059845657857 18.78442461100445 62-63 19.371855346126168 30.868577753350962 30.714772360552555 19.04479453997032 64-65 19.236566705051736 30.887270702750197 30.6749260913749 19.201236500823168 66-67 19.123916325150706 30.46237163023548 31.20762257194702 19.206089472666797 68-69 18.9465428546748 30.036217562376095 31.90044573029351 19.1167938526556 70-71 18.777255263696368 29.682848005826767 32.32260113882144 19.21729559165543 72-73 18.701328265395958 29.505837032480425 32.364057537079624 19.42877716504399 74-75 18.930984031084545 29.56357126302472 31.966593509922063 19.53885119596867 76-77 19.091037170792717 29.77276928732343 31.594037148422927 19.542156393460917 78-79 19.322958549186456 30.17744126978566 30.829102765377915 19.670497415649972 80-81 19.454030372683373 30.478109504861685 30.188456569067363 19.87940355338758 82-83 19.60079074135327 30.74460169197049 29.461398575345697 20.19320899133054 84-85 19.5609106780175 30.95512090172152 28.977585080683426 20.506383339577553 86-87 19.577824818178797 31.03205646447229 28.61876759358547 20.77135112376344 88-89 19.571066906474417 31.098988626590103 28.324634278130766 21.005310188804714 90-91 19.553281017248114 31.13754811727586 27.942790696964437 21.366380168511593 92-93 19.577884139985564 31.11842578957111 27.689341632779147 21.614348437664184 94-95 19.677334608701628 31.058408166258545 27.541137851821674 21.723119373218154 96-97 19.74422348627869 31.037910771173983 27.363220943874587 21.854644798672744 98-99 19.824964449719523 31.124973276647665 27.132018393588474 21.918043880044337 100 19.850147539644343 31.07198023219772 26.9996659185038 22.07820630965413 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 50711.0 1 38133.0 2 20906.0 3 15390.0 4 14647.5 5 14980.5 6 15667.5 7 16053.5 8 16214.0 9 17382.0 10 20322.0 11 41140.5 12 285972.5 13 344302.5 14 177144.5 15 191646.0 16 220061.5 17 245965.0 18 267588.5 19 289139.0 20 313543.5 21 342541.0 22 376589.5 23 407999.5 24 432816.5 25 460702.5 26 492725.0 27 533096.0 28 580827.0 29 615866.5 30 641956.0 31 675609.0 32 707561.0 33 728793.0 34 735084.0 35 725626.0 36 715354.0 37 700557.5 38 683838.5 39 668068.5 40 651946.0 41 644193.5 42 640621.0 43 626499.0 44 609343.5 45 606229.5 46 605069.0 47 601707.5 48 599243.0 49 599125.5 50 604059.5 51 611307.5 52 615912.5 53 611572.0 54 600177.0 55 587248.0 56 570890.5 57 541966.5 58 499495.5 59 445488.0 60 378084.0 61 315609.0 62 261894.0 63 207360.5 64 159311.0 65 120242.0 66 89957.5 67 69129.0 68 54547.0 69 42363.5 70 32539.5 71 25983.5 72 21213.0 73 16760.5 74 13440.0 75 11111.0 76 8292.5 77 5265.5 78 3214.5 79 2074.0 80 1292.5 81 795.0 82 489.0 83 288.5 84 183.5 85 132.0 86 82.5 87 55.0 88 41.0 89 28.0 90 20.5 91 20.0 92 15.0 93 6.0 94 5.0 95 4.5 96 2.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3303643895279941 2 0.006309225035019467 3 2.397865106444109 4 7.990958593576757E-4 5 2.5425777343198777E-5 6 0.008448622585754337 7 0.007609571933428777 8 3.3416735936775535E-4 9 0.0 10-11 8.354183984193884E-5 12-13 0.0 14-15 0.0 16-17 2.3609650390113152E-5 18-19 0.0 20-21 2.86948058587529E-4 22-23 3.813866601479816E-5 24-25 4.885381503800336E-4 26-27 0.0033997896561762933 28-29 0.005978689929557884 30-31 0.005522842064333391 32-33 0.014946724823894711 34-35 0.009629105105259994 36-37 0.005090603849499013 38-39 0.005350310003790257 40-41 0.008261561509586518 42-43 0.018791465583576982 44-45 0.033305952192637316 46-47 0.03270299804421289 48-49 0.03949712897570622 50-51 0.03918475513977549 52-53 0.061819145356081656 54-55 0.0329409106750671 56-57 0.022075023114755798 58-59 0.016775564665651936 60-61 0.01791609239218971 62-63 0.024417826884236254 64-65 0.044199081657244906 66-67 0.04722474916108556 68-69 0.05013236841297564 70-71 0.0606768015025908 72-73 0.06830271857859734 74-75 0.05892242286591008 76-77 0.059301993399104976 78-79 0.054164170248825735 80-81 0.05164338603794289 82-83 0.04773144858099645 84-85 0.04962203673915858 86-87 0.05724432156125896 88-89 0.06424185871149787 90-91 0.0601828149713515 92-93 0.06462506149859895 94-95 0.07303554541833848 96-97 0.07246164930116343 98-99 0.04739364896772252 100 0.006915811437350068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7531115E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.88092787922799 #Duplication Level Percentage of deduplicated Percentage of total 1 67.2438376871097 23.455274526865868 2 17.739315753109853 12.375275868221552 3 6.154651261496054 6.440398403221302 4 2.7320345681109393 3.811836029353419 5 1.4531457307767706 2.534353571661629 6 0.9476114193587735 1.983213934571175 7 0.6379012903785842 1.5575412232633308 8 0.45614614890628824 1.2728640737910266 9 0.3425842378377039 1.0754690483319516 >10 1.9657416034850625 13.191715609650883 >50 0.22250837612699348 5.325576312192183 >100 0.09741143111285716 5.90924081545135 >500 0.0036335754674705675 0.8714179755642524 >1k 0.0028721331205473653 1.9645409288962559 >5k 3.4410101493275174E-4 0.8426926518354345 >10k+ 2.606825870694544E-4 17.388589027128255 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4164683 15.127186094715018 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 101517 0.3687355197927872 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 88182 0.3202994139539935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51287 0.1862874060858051 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA 31888 0.11582531255998893 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 31480 0.11434335296627107 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017507463827745443 3.6322539061712538E-6 0.0 3.6322539061712538E-6 4.358704687405505E-5 2 0.001758010890586887 7.2645078123425075E-6 0.0 3.6322539061712538E-6 4.358704687405505E-5 3 0.001758010890586887 7.2645078123425075E-6 3.6322539061712538E-6 7.2645078123425075E-6 4.72193007802263E-5 4 0.001758010890586887 1.4529015624685015E-5 3.6322539061712538E-6 7.2645078123425075E-6 5.448380859256881E-5 5 0.001758010890586887 2.905803124937003E-5 3.6322539061712538E-6 7.2645078123425075E-6 5.448380859256881E-5 6 0.001758010890586887 2.905803124937003E-5 3.6322539061712538E-6 1.0896761718513762E-5 6.174831640491131E-5 7 0.001758010890586887 2.905803124937003E-5 3.6322539061712538E-6 1.0896761718513762E-5 6.901282421725382E-5 8 0.001758010890586887 3.632253906171254E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.354183984193884E-5 9 0.001758010890586887 3.995479296788379E-5 3.6322539061712538E-6 1.0896761718513762E-5 8.354183984193884E-5 10-11 0.0017689076523054008 3.995479296788379E-5 5.448380859256881E-6 1.0896761718513762E-5 8.535796679502447E-5 12-13 0.0018052301913671132 4.177091992096942E-5 7.2645078123425075E-6 1.0896761718513762E-5 9.080634765428134E-5 14-15 0.001865162380818939 5.266768163948318E-5 7.2645078123425075E-6 1.2712888671599388E-5 1.0170310937279511E-4 16-17 0.001952336474567049 5.448380859256881E-5 7.2645078123425075E-6 1.4529015624685015E-5 1.0170310937279511E-4 18-19 0.002034062187455902 5.993218945182569E-5 7.2645078123425075E-6 1.816126953085627E-5 1.1804825195056574E-4 20-21 0.0021793523437027523 6.901282421725382E-5 7.2645078123425075E-6 2.905803124937003E-5 1.2531275976290825E-4 22-23 0.002419081101510055 7.264507812342508E-5 7.2645078123425075E-6 3.2690285155541284E-5 1.2712888671599388E-4 24-25 0.0029675514413419145 8.899022070119571E-5 7.2645078123425075E-6 3.2690285155541284E-5 1.2712888671599388E-4 26-27 0.0042642660858450525 1.0533536327896636E-4 7.2645078123425075E-6 3.2690285155541284E-5 1.3257726757525076E-4 28-29 0.007337152890465933 1.1623212499748012E-4 7.2645078123425075E-6 4.5403173827140675E-5 1.416579023406789E-4 30-31 0.013531961927441006 1.1986437890365138E-4 7.2645078123425075E-6 5.448380859256881E-5 1.5255466405919267E-4 32-33 0.02491907792328789 1.2168050585673699E-4 1.4529015624685015E-5 5.811606249874006E-5 1.5800304491844953E-4 34-35 0.044794771297856986 1.2349663280982262E-4 1.4529015624685015E-5 5.811606249874006E-5 1.7071593359004893E-4 36-37 0.07551637483625345 1.343933945283364E-4 1.4529015624685015E-5 5.811606249874006E-5 1.8524494921473396E-4 38-39 0.12050002333723134 1.343933945283364E-4 1.4529015624685015E-5 6.174831640491131E-5 1.888772031209052E-4 40-41 0.18032142904491882 1.3620952148142202E-4 1.4529015624685015E-5 6.538057031108257E-5 1.888772031209052E-4 42-43 0.25517673367024907 1.471062831999358E-4 1.4529015624685015E-5 6.538057031108257E-5 1.9250945702707644E-4 44-45 0.34711089616239665 1.5618691796536393E-4 1.4529015624685015E-5 6.538057031108257E-5 1.9977396483941896E-4 46-47 0.46167944887085033 1.6526755273079205E-4 1.4529015624685015E-5 6.538057031108257E-5 2.0703847265176147E-4 48-49 0.598749814528035 1.7253206054313456E-4 1.4529015624685015E-5 6.538057031108257E-5 2.161191074171896E-4 50-51 0.7594770498761129 1.7616431444930582E-4 1.4529015624685015E-5 6.538057031108257E-5 2.2883199608878898E-4 52-53 0.9362170765695468 1.7798044140239144E-4 1.4529015624685015E-5 6.901282421725382E-5 2.3972875780730276E-4 54-55 1.1340241759187741 1.8342882226164833E-4 1.4529015624685015E-5 6.901282421725382E-5 2.3972875780730276E-4 56-57 1.354836155382737 1.888772031209052E-4 1.4529015624685015E-5 7.082895117033945E-5 2.4154488476038838E-4 58-59 1.6044718857191218 1.961417109332477E-4 1.4529015624685015E-5 7.264507812342508E-5 2.5607390038507336E-4 60-61 1.8775774246702324 2.0340621874559021E-4 1.4529015624685015E-5 7.264507812342508E-5 2.57890027338159E-4 62-63 2.167485770191291 2.0340621874559021E-4 1.6345142577770642E-5 7.264507812342508E-5 2.706029160097584E-4 64-65 2.4706518424698745 2.1430298046410398E-4 1.816126953085627E-5 7.446120507651071E-5 2.760512968690153E-4 66-67 2.7982539029022253 2.215674882764465E-4 1.816126953085627E-5 7.627733202959634E-5 2.833158046813578E-4 68-69 3.1503173772656865 2.215674882764465E-4 1.816126953085627E-5 7.990958593576758E-5 2.851319316344434E-4 70-71 3.5257834635466088 2.2701586913570336E-4 1.816126953085627E-5 8.354183984193884E-5 3.0329320116529975E-4 72-73 3.915682310723703 2.43361011713474E-4 1.816126953085627E-5 8.354183984193884E-5 3.0692545507147095E-4 74-75 4.321036398271556 2.43361011713474E-4 1.816126953085627E-5 8.71740937481101E-5 3.087415820245566E-4 76-77 4.745902953803361 2.488093925727309E-4 1.816126953085627E-5 9.080634765428135E-5 3.1418996288381346E-4 78-79 5.186918510202002 2.5970615429124467E-4 1.99773964839419E-5 9.44386015604526E-5 3.287189785084985E-4 80-81 5.647951417877554 2.7241904296284404E-4 2.1793523437027523E-5 9.807085546662385E-5 3.450641210862691E-4 82-83 6.115235434525626 2.7968355077518655E-4 2.1793523437027523E-5 9.807085546662385E-5 3.5414475585169723E-4 84-85 6.5949290466441335 3.032932011652997E-4 2.1793523437027523E-5 1.0533536327896636E-4 3.5959313671095415E-4 86-87 7.0892679065123225 3.1600608983689907E-4 2.1793523437027523E-5 1.0533536327896636E-4 3.6685764452329666E-4 88 7.468062953498251 3.1600608983689907E-4 2.1793523437027523E-5 1.0533536327896636E-4 3.704898984294679E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 654480 0.0 92.29462 1 AGCAGTG 692620 0.0 89.09256 2 GCAGTGG 707175 0.0 87.52328 3 AGTGGTA 744480 0.0 83.38605 5 CAGTGGT 741760 0.0 83.272514 4 GTGGTAT 749460 0.0 82.83009 6 TGGTATC 757985 0.0 82.39978 7 GGTATCA 762380 0.0 81.98437 8 GTATCAA 769890 0.0 81.0566 9 TAGCAGT 22145 0.0 57.628143 1 AGTACTT 664165 0.0 44.36059 20-21 GAGTACT 661615 0.0 43.27006 20-21 TACTTTT 664605 0.0 43.17812 22-23 GTACTTT 653105 0.0 42.71684 22-23 AGAGTAC 737390 0.0 42.664944 18-19 ACGCAGA 755015 0.0 42.59385 14-15 ATCAACG 764495 0.0 42.13594 10-11 CAACGCA 764170 0.0 42.013195 12-13 GCAGAGT 765790 0.0 41.957184 16-17 AACGCAG 754320 0.0 41.26346 14-15 >>END_MODULE