FastQCFastQC Report
Thu 26 May 2016
SRR1660742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26592900
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11698034.398929789530288No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC579390.21787394379702854Illumina Single End PCR Primer 1 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT308910.11616258475006487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1510600.084.264098
AGTGGTA1530250.083.4595955
GTGGTAT1543650.082.686286
GTATCAA1543150.082.392059
TGGTATC1542900.082.336027
AAGCAGT1608800.079.845191
GCAGTGG1618100.079.4080353
CAGTGGT1634450.078.118934
AGCAGTG1688000.076.107542
CAACGCA1482200.044.4408612-13
ATCAACG1495600.044.11164510-11
ACGCAGA1510950.043.62551514-15
TCAACGC1483350.043.00736612-13
AGAGTAC1513700.042.98241418-19
AACGCAG1500600.042.60911614-15
GAGTACT1477000.042.36515420-21
CGCAGAG1520650.041.96175816-17
TATCAAC1538200.041.33015410-11
AGTACTT1567600.041.1985720-21
GTACTTT1574850.039.69801322-23
Produced by FastQC (version 0.11.5)