##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660742_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26592900 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99816296831109 34.0 31.0 34.0 30.0 34.0 2 32.136270282669436 34.0 31.0 34.0 30.0 34.0 3 32.189423492736786 34.0 31.0 34.0 30.0 34.0 4 35.604799062907766 37.0 35.0 37.0 33.0 37.0 5 35.58246065679185 37.0 35.0 37.0 33.0 37.0 6 35.6472423466414 37.0 35.0 37.0 33.0 37.0 7 35.60948851009104 37.0 35.0 37.0 33.0 37.0 8 35.61627220047456 37.0 35.0 37.0 33.0 37.0 9 37.31843992193405 39.0 38.0 39.0 34.0 39.0 10-11 37.25865768682618 39.0 37.0 39.0 34.0 39.0 12-13 37.27670831312117 39.0 37.0 39.0 34.0 39.0 14-15 38.67167689496068 40.0 38.0 41.0 34.0 41.0 16-17 38.6556896765678 40.0 38.0 41.0 34.0 41.0 18-19 38.623262863395865 40.0 38.5 41.0 34.0 41.0 20-21 38.519948971341975 40.0 38.5 41.0 34.0 41.0 22-23 38.371667324737054 40.0 38.0 41.0 34.0 41.0 24-25 38.35466996829981 40.0 38.0 41.0 34.0 41.0 26-27 38.329730435567384 40.0 38.0 41.0 34.0 41.0 28-29 38.24326182928526 40.0 38.0 41.0 33.0 41.0 30-31 38.1416398738009 40.0 38.0 41.0 33.0 41.0 32-33 38.02019283342547 40.0 38.0 41.0 33.0 41.0 34-35 37.88116356245464 40.0 38.0 41.0 32.5 41.0 36-37 37.71199869138003 40.0 38.0 41.0 32.0 41.0 38-39 37.351376288407806 40.0 36.0 41.0 31.5 41.0 40-41 37.28363856518093 40.0 36.0 41.0 31.0 41.0 42-43 37.26427279461811 40.0 36.0 41.0 31.0 41.0 44-45 37.241203291104014 40.0 36.0 41.0 31.0 41.0 46-47 37.29195364176152 40.0 36.0 41.0 31.0 41.0 48-49 37.19200882566399 40.0 36.0 41.0 31.0 41.0 50-51 35.79947732289446 38.5 34.5 40.0 29.0 40.5 52-53 35.98248226030256 38.5 35.0 39.5 29.5 40.5 54-55 36.502079558829614 39.5 35.0 41.0 29.5 41.0 56-57 36.22720192231761 40.0 35.0 41.0 28.0 41.0 58-59 36.07429338282023 39.0 35.0 41.0 27.5 41.0 60-61 35.855928800544504 39.0 35.0 41.0 27.0 41.0 62-63 35.62175191498483 39.0 35.0 41.0 26.5 41.0 64-65 35.34025012315317 38.0 35.0 41.0 26.0 41.0 66-67 35.02248267394681 37.0 34.5 40.0 26.0 41.0 68-69 34.71503931500513 37.0 34.0 40.0 26.0 41.0 70-71 34.40623094886229 36.0 34.0 39.0 26.0 41.0 72-73 34.061002128387656 36.0 34.0 39.0 25.0 41.0 74-75 33.68445197778354 35.0 34.0 38.5 25.0 40.5 76-77 33.29593186527231 35.0 33.0 37.0 24.0 39.5 78-79 32.923288546942985 35.0 33.0 37.0 23.5 39.0 80-81 32.586658318573754 35.0 33.0 36.5 23.0 39.0 82-83 32.28971789462601 35.0 33.0 36.0 22.5 38.0 84-85 32.004969879178276 35.0 33.0 36.0 21.0 37.0 86-87 31.790810968341177 35.0 32.5 35.0 20.0 37.0 88-89 31.587840250593203 35.0 32.0 35.0 20.0 36.0 90-91 31.41012642472239 35.0 32.0 35.0 20.0 36.0 92-93 31.263183744533315 35.0 32.0 35.0 19.5 36.0 94-95 31.092783581331858 35.0 32.0 35.0 19.0 36.0 96-97 30.961719707139878 35.0 31.5 35.0 18.5 35.0 98-99 30.856210210244086 35.0 31.0 35.0 18.0 35.0 100 30.771888022742914 35.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47651755971522647 1101 2 0.5107052647141472 1101 3 0.5146132009420796 1101 4 0.42911389757732366 1101 5 0.43456299594338077 1101 6 0.40314080446863443 1101 7 0.439404672595046 1101 8 0.4368388118142832 1101 9 0.504000940445529 1101 10-11 0.5283478011750233 1101 12-13 0.5044472272432259 1101 14-15 0.6149787447482922 1101 16-17 0.6004202654609756 1101 18-19 0.6033189055497203 1101 20-21 0.6117966188947079 1101 22-23 0.638174660195844 1101 24-25 0.6443092218459938 1101 26-27 0.6277413056846868 1101 28-29 0.6062771087653331 1101 30-31 0.6088446624017365 1101 32-33 0.5834014339903391 1101 34-35 0.5688067085320725 1101 36-37 0.5344394405009751 1101 38-39 0.5491179617251376 1101 40-41 0.5603053950330334 1101 42-43 0.5567215956318989 1101 44-45 0.5899550029651763 1101 46-47 0.5914704709978764 1101 48-49 0.6390280091180003 1101 50-51 0.6005825766013615 1101 52-53 0.6236702749858125 1101 54-55 0.7087061650743252 1101 56-57 0.7088454558122592 1101 58-59 0.6322145534095895 1101 60-61 0.6630489850610388 1101 62-63 0.6287011035950414 1101 64-65 0.5948079184725898 1101 66-67 0.5729321824169844 1101 68-69 0.5568834935093463 1101 70-71 0.5426107371192046 1101 72-73 0.5113375804285099 1101 74-75 0.5035090685490218 1101 76-77 0.5015489273616325 1101 78-79 0.47570670933878745 1101 80-81 0.4486795255467513 1101 82-83 0.403380887929913 1101 84-85 0.3973011672628175 1101 86-87 0.3963470867292358 1101 88-89 0.3615000081376536 1101 90-91 0.38147451412469024 1101 92-93 0.3755632544600225 1101 94-95 0.3748315988857307 1101 96-97 0.3728987839056721 1101 98-99 0.3907827875630261 1101 100 0.3426848930480695 1102 1 0.3823670989359442 1102 2 0.41235650541075586 1102 3 0.41093860038117924 1102 4 0.3336515664237609 1102 5 0.34757618671219603 1102 6 0.3196003485615577 1102 7 0.31869890068877993 1102 8 0.3495479935003516 1102 9 0.3872099187187956 1102 10-11 0.38284979967873056 1102 12-13 0.4033317071543081 1102 14-15 0.4844089131096396 1102 16-17 0.44111262015874075 1102 18-19 0.42438140320631135 1102 20-21 0.4448748519650465 1102 22-23 0.49275303452854047 1102 24-25 0.43187289779896787 1102 26-27 0.44234932130014215 1102 28-29 0.439682397906779 1102 30-31 0.4305472247996889 1102 32-33 0.43720329977959693 1102 34-35 0.4219647820413215 1102 36-37 0.4390837479234051 1102 38-39 0.4618098239815609 1102 40-41 0.44776498809176246 1102 42-43 0.4331148021664646 1102 44-45 0.42522658131536417 1102 46-47 0.43410903224703645 1102 48-49 0.49130667611848367 1102 50-51 0.44944178546960245 1102 52-53 0.4687141655417193 1102 54-55 0.491270385145377 1102 56-57 0.5074008790288573 1102 58-59 0.4105600333236268 1102 60-61 0.39144804137977474 1102 62-63 0.3883506513717023 1102 64-65 0.3719230572364154 1102 66-67 0.36403864604502445 1102 68-69 0.36733732768475846 1102 70-71 0.3642723126806686 1102 72-73 0.3427951205194333 1102 74-75 0.3368717348395762 1102 76-77 0.3005868612001734 1102 78-79 0.33741420294759195 1102 80-81 0.27143874208365304 1102 82-83 0.26204959288617147 1102 84-85 0.27918059072096213 1102 86-87 0.24981395167186804 1102 88-89 0.2081729406757873 1102 90-91 0.19384021463774204 1102 92-93 0.1837560101472313 1102 94-95 0.2262613624486427 1102 96-97 0.1831180445867595 1102 98-99 0.1606741706061996 1102 100 0.14601495440623324 1103 1 0.3631940362838364 1103 2 0.3884229587680821 1103 3 0.38497547069139415 1103 4 0.3319969972161445 1103 5 0.34045085987260393 1103 6 0.3413249454571954 1103 7 0.34808510556420913 1103 8 0.35245072974918656 1103 9 0.3985793592511868 1103 10-11 0.399205679191148 1103 12-13 0.39787222030685143 1103 14-15 0.46515476958366264 1103 16-17 0.44308346659654063 1103 18-19 0.43370397118033566 1103 20-21 0.49151465114589854 1103 22-23 0.4802801582596601 1103 24-25 0.49090552963276224 1103 26-27 0.47683831843632873 1103 28-29 0.47022105647221224 1103 30-31 0.4239643519124101 1103 32-33 0.3770011990917652 1103 34-35 0.42683782542899706 1103 36-37 0.42035234857000603 1103 38-39 0.42671572025923155 1103 40-41 0.4052086892264839 1103 42-43 0.42555818836562764 1103 44-45 0.44130414686198804 1103 46-47 0.470339544106011 1103 48-49 0.48977107588550695 1103 50-51 0.3915497502650638 1103 52-53 0.42579928924111954 1103 54-55 0.48947409493594307 1103 56-57 0.5346013860224943 1103 58-59 0.4559708115158685 1103 60-61 0.490070042342019 1103 62-63 0.4757009245050128 1103 64-65 0.42486429416950244 1103 66-67 0.40672982602288243 1103 68-69 0.3958077183679549 1103 70-71 0.3827746903689828 1103 72-73 0.3883027207262515 1103 74-75 0.3940068216749779 1103 76-77 0.3723692175780968 1103 78-79 0.3443551045757758 1103 80-81 0.2911706180495557 1103 82-83 0.3028873902494169 1103 84-85 0.29607057538683534 1103 86-87 0.25972168344508617 1103 88-89 0.29282040526789643 1103 90-91 0.2837315141407828 1103 92-93 0.22241846341707472 1103 94-95 0.30366823867197823 1103 96-97 0.24522214559643984 1103 98-99 0.2634503200477063 1103 100 0.2253894262408842 1104 1 0.3910413668258883 1104 2 0.4055577433786013 1104 3 0.41268769508741343 1104 4 0.34400789324119074 1104 5 0.34703340359074986 1104 6 0.29108660491164073 1104 7 0.31115473779814096 1104 8 0.3437709200430987 1104 9 0.3901593629014215 1104 10-11 0.3789785593373054 1104 12-13 0.37190216729349856 1104 14-15 0.44416374196215 1104 16-17 0.4329196028182096 1104 18-19 0.4009140097819994 1104 20-21 0.38560625870725573 1104 22-23 0.4137646304463516 1104 24-25 0.42895453968817066 1104 26-27 0.4187408282572207 1104 28-29 0.3996660344124834 1104 30-31 0.38570336743205047 1104 32-33 0.3681166027162419 1104 34-35 0.3516567416532297 1104 36-37 0.3475949561235012 1104 38-39 0.34809479796234655 1104 40-41 0.38689004022949547 1104 42-43 0.3250174698002155 1104 44-45 0.36209178118099317 1104 46-47 0.4007510177120963 1104 48-49 0.4354355198680864 1104 50-51 0.35439325000479727 1104 52-53 0.3782979353616156 1104 54-55 0.47875391418859436 1104 56-57 0.5260128253249547 1104 58-59 0.4972041712182218 1104 60-61 0.48112405954239534 1104 62-63 0.4355460690787467 1104 64-65 0.43851486126114736 1104 66-67 0.43844127096839713 1104 68-69 0.43469043152369835 1104 70-71 0.41412244045190505 1104 72-73 0.4081680383307642 1104 74-75 0.3713037635807268 1104 76-77 0.3215038236428214 1104 78-79 0.3543988152444726 1104 80-81 0.3153357877310299 1104 82-83 0.330297320123222 1104 84-85 0.33962758928286263 1104 86-87 0.2891969658115059 1104 88-89 0.27498952341379024 1104 90-91 0.24153644944993857 1104 92-93 0.2690330618807302 1104 94-95 0.33301249410017775 1104 96-97 0.322016821341343 1104 98-99 0.29988177373542513 1104 100 0.20325437478581065 1105 1 0.24198820159627843 1105 2 0.29542429686908633 1105 3 0.27206462292454603 1105 4 0.21997881309782485 1105 5 0.2349267288925745 1105 6 0.2145978899877221 1105 7 0.24799436841962574 1105 8 0.2645047838296719 1105 9 0.2516763875325623 1105 10-11 0.28201132835442166 1105 12-13 0.25883152985273483 1105 14-15 0.3019564377442876 1105 16-17 0.29465592284943654 1105 18-19 0.26989749139661257 1105 20-21 0.28228484654243147 1105 22-23 0.32113254314761974 1105 24-25 0.35332065735513396 1105 26-27 0.30802048761538714 1105 28-29 0.3039911049931021 1105 30-31 0.3183799000791723 1105 32-33 0.2936069058756132 1105 34-35 0.31388799854278915 1105 36-37 0.3106084210499205 1105 38-39 0.33141193191136153 1105 40-41 0.34882123094613604 1105 42-43 0.33142903926995615 1105 44-45 0.37143271472735506 1105 46-47 0.3524059422487369 1105 48-49 0.37002665467712603 1105 50-51 0.24589955223418514 1105 52-53 0.3303482003557576 1105 54-55 0.4461289441075067 1105 56-57 0.44152120991267907 1105 58-59 0.41619986535229003 1105 60-61 0.3706926559605961 1105 62-63 0.38951986789503934 1105 64-65 0.3769740234086143 1105 66-67 0.3647819218401551 1105 68-69 0.3577556134457751 1105 70-71 0.3680407914826489 1105 72-73 0.33503447129286457 1105 74-75 0.3225957817692944 1105 76-77 0.31205458267231023 1105 78-79 0.31321219187853444 1105 80-81 0.3298576840247449 1105 82-83 0.31993406286360937 1105 84-85 0.29741016689381894 1105 86-87 0.27122004817846346 1105 88-89 0.29491980380952754 1105 90-91 0.26156923845892877 1105 92-93 0.26559485984891396 1105 94-95 0.3315918129914657 1105 96-97 0.29343926482167504 1105 98-99 0.2701691101161501 1105 100 0.2712663727119704 1106 1 0.19420736317045595 1106 2 0.22810259021230905 1106 3 0.2247907013730881 1106 4 0.20465391175016379 1106 5 0.21906947880211192 1106 6 0.179619979898888 1106 7 0.19860593028041507 1106 8 0.21149659193463322 1106 9 0.21731049255631518 1106 10-11 0.20043514416600772 1106 12-13 0.2141321296677532 1106 14-15 0.2707215738393103 1106 16-17 0.2265259082437936 1106 18-19 0.21038082043054374 1106 20-21 0.2431614861770086 1106 22-23 0.2616154370964665 1106 24-25 0.2761129007351073 1106 26-27 0.2670894609096237 1106 28-29 0.266859041301565 1106 30-31 0.24349356285288337 1106 32-33 0.2654537473689942 1106 34-35 0.3008012451192954 1106 36-37 0.2516482063571459 1106 38-39 0.30369539655810485 1106 40-41 0.2944571643043048 1106 42-43 0.2835211666843307 1106 44-45 0.3306681134114413 1106 46-47 0.30102491210335813 1106 48-49 0.3016951628528801 1106 50-51 0.1643182706804538 1106 52-53 0.2505829960241215 1106 54-55 0.4067029889321603 1106 56-57 0.4449729765521724 1106 58-59 0.3709134697372818 1106 60-61 0.34600826447086064 1106 62-63 0.3593481672282408 1106 64-65 0.3314971261688626 1106 66-67 0.32978561146858 1106 68-69 0.3344488914125918 1106 70-71 0.3492658333035621 1106 72-73 0.3369492281031583 1106 74-75 0.32280232505762285 1106 76-77 0.3211429911411656 1106 78-79 0.3352558351814139 1106 80-81 0.28983152438870974 1106 82-83 0.3223040927114553 1106 84-85 0.308811055666105 1106 86-87 0.2800306563443371 1106 88-89 0.3050783597771982 1106 90-91 0.2743552916009051 1106 92-93 0.23499445152415177 1106 94-95 0.2738600770780799 1106 96-97 0.3113831394924276 1106 98-99 0.27069152212614966 1106 100 0.2682517459847986 1107 1 0.17848494026534212 1107 2 0.19487687215818283 1107 3 0.19574218839017732 1107 4 0.22255499562342607 1107 5 0.20638402165114655 1107 6 0.19560816133725467 1107 7 0.20368557636055584 1107 8 0.19609219891582086 1107 9 0.20367233111799976 1107 10-11 0.20243064045171622 1107 12-13 0.2001485772555327 1107 14-15 0.1940682089768302 1107 16-17 0.2086224985174212 1107 18-19 0.2045070893897929 1107 20-21 0.19938825701351703 1107 22-23 0.22112928109268637 1107 24-25 0.23850730132618736 1107 26-27 0.22475663465559848 1107 28-29 0.2258537066071611 1107 30-31 0.20755460466455844 1107 32-33 0.16666024243630773 1107 34-35 0.19997990832974466 1107 36-37 0.18841300236425695 1107 38-39 0.22550057164628612 1107 40-41 0.21584107790430807 1107 42-43 0.2551914630005854 1107 44-45 0.3051080301983191 1107 46-47 0.27924862272557505 1107 48-49 0.31995906553101605 1107 50-51 0.23159189336801234 1107 52-53 0.2518462948945057 1107 54-55 0.34100930177455524 1107 56-57 0.35972041211164907 1107 58-59 0.29180603847445497 1107 60-61 0.3051340780427836 1107 62-63 0.2608426946036744 1107 64-65 0.23922475781383667 1107 66-67 0.28481971096044845 1107 68-69 0.26224459721503734 1107 70-71 0.25189123659842494 1107 72-73 0.25688253846026754 1107 74-75 0.27237612264904243 1107 76-77 0.2723016251928314 1107 78-79 0.25199579276247874 1107 80-81 0.22075871541697722 1107 82-83 0.23615450092260204 1107 84-85 0.24043221499669443 1107 86-87 0.2350759223591723 1107 88-89 0.23922318900152106 1107 90-91 0.2650473659432535 1107 92-93 0.2682616500297108 1107 94-95 0.2667873291198184 1107 96-97 0.26275612693857653 1107 98-99 0.2937994706038687 1107 100 0.2781640621076136 1108 1 0.14612086973716742 1108 2 0.16768636018677086 1108 3 0.1783983542519323 1108 4 0.15637675916548233 1108 5 0.17102352523320974 1108 6 0.12304994349774034 1108 7 0.11845604391798759 1108 8 0.1276790088624793 1108 9 0.14642585861805912 1108 10-11 0.10688071544341682 1108 12-13 0.14784434820921177 1108 14-15 0.18704390920005665 1108 16-17 0.16776302250101338 1108 18-19 0.15668113986791354 1108 20-21 0.16218038139914626 1108 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2316 56-57 -1.4532447338235386 2316 58-59 -1.241383914496076 2316 60-61 -1.1906487477238414 2316 62-63 -1.2385283729393919 2316 64-65 -1.3579064053586691 2316 66-67 -1.2917426873771873 2316 68-69 -1.180462998567819 2316 70-71 -1.593170610411633 2316 72-73 -1.2849454011451158 2316 74-75 -1.2043575072771944 2316 76-77 -1.1241523882805424 2316 78-79 -0.9362042167397533 2316 80-81 -0.9524262158864865 2316 82-83 -0.9021457901493228 2316 84-85 -0.9958663379581338 2316 86-87 -1.0847114948344654 2316 88-89 -1.0098112453812575 2316 90-91 -0.9509389297227386 2316 92-93 -0.8716353200111975 2316 94-95 -1.0630862581651357 2316 96-97 -1.1849678470015448 2316 98-99 -1.1153409037973745 2316 100 -1.0227515177959532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11332.0 3 2837.0 4 2006.0 5 1709.0 6 2190.0 7 7546.0 8 16216.0 9 21880.0 10 24838.0 11 25210.0 12 27091.0 13 30326.0 14 36690.0 15 45798.0 16 59513.0 17 74711.0 18 82471.0 19 92298.0 20 106330.0 21 130342.0 22 165910.0 23 241027.0 24 347434.0 25 423315.0 26 433705.0 27 392604.0 28 346911.0 29 347061.0 30 390107.0 31 473735.0 32 593268.0 33 773403.0 34 1068731.0 35 1644064.0 36 2884695.0 37 5615632.0 38 7338572.0 39 2306675.0 40 4717.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.210806008655284 32.38841518943009 27.179915092627084 25.22086370928754 2 20.672134091770037 31.831057922609872 29.67405609191794 17.822751893702147 3 23.72564162804461 26.5026220113368 27.37774687126131 22.393989489357274 4 18.377381066037422 28.602917735071102 26.175465197272796 26.844236001618683 5 21.581057865468797 31.995455220465907 26.834495849091304 19.58899106497399 6 26.877655495559893 27.83787757126192 26.487531050333253 18.796935882844934 7 21.064123784526423 26.936275443642494 31.74657580147201 20.25302497035908 8 25.1252814387314 27.03896880464785 27.159445157431556 20.67630459918919 9 24.676391042458576 25.54136870477582 28.80959084087617 20.97264941188943 10-11 20.588243930662863 29.513213330663447 29.319281257937526 20.579261480736164 12-13 20.617112462654884 26.982193195566524 29.03048751311566 23.37020682866293 14-15 20.593390751099545 32.10432851517096 26.65821838918397 20.644062344545528 16-17 23.2560588980013 26.79175484005148 26.58611755157818 23.36606871036904 18-19 20.640712980365624 29.38804196703006 26.736114190059627 23.235130862544686 20-21 23.110579041389407 29.633967751683183 26.692217985253762 20.563235221673647 22-23 23.355140204328546 26.948596272347896 29.09790647526816 20.598357048055398 24-25 20.773398619345947 29.47914260851285 26.389909129189448 23.357549642951753 26-27 20.65621806587112 27.103044019259215 31.62570787809021 20.615030036779462 28-29 20.75893800127377 27.09662383416199 31.582197678104464 20.562240486459782 30-31 20.721951833809648 26.85035878943748 31.721559833181722 20.70612954357115 32-33 20.84869417197464 26.98785020195153 31.518202453828753 20.645253172245077 34-35 20.869635368979754 27.240472083502652 31.273800761172716 20.616091786344874 36-37 20.683949237732932 27.202884808031452 31.44177380888602 20.671392145349596 38-39 20.711975461977676 27.037402705156673 31.491504476212473 20.759117356653178 40-41 20.884369463931183 27.083104932711997 31.317662801987023 20.714862801369797 42-43 20.905307915824224 27.111602125301847 31.359905137141915 20.623184821732014 44-45 21.072593711520607 27.186961462504687 31.23174430435841 20.508700521616298 46-47 20.855642461594513 27.289789256550627 31.257275728078 20.597292553776864 48-49 21.050692434247757 27.282356995929163 30.994950387382453 20.672000182440627 50-51 21.097232056224144 27.346058428846693 30.920897077251723 20.635812437677433 52-53 21.102593629052055 27.58167751218884 30.834929569280128 20.48079928947898 54-55 21.40325921496401 27.663935702667935 30.196886510218913 20.735918572149142 56-57 21.8363275789474 28.22466964713068 29.293926762906025 20.645076011015895 58-59 21.982901585459754 28.663458906568255 28.755239222832184 20.598400285139807 60-61 22.204073615276208 28.986849129700204 28.276792122767326 20.532285132256263 62-63 22.298111588614443 29.11963331217009 28.095688613588386 20.48656648562708 64-65 22.438259487484682 29.178370476593642 27.99817701266495 20.385193023256733 66-67 22.580908964309586 29.00394893448446 28.106462284448746 20.308679816757213 68-69 22.72289512342426 28.83527663541456 28.267113158132638 20.17471508302854 70-71 22.915419711120396 28.684156041664316 28.32836056872466 20.07206367849063 72-73 23.089957948983592 28.597896203295974 28.292920095465547 20.01922575225489 74-75 23.331311445884147 28.641199416506996 28.173631513741277 19.853857623867583 76-77 23.640427324933025 28.700325608880927 27.892377672425262 19.76686939376079 78-79 23.92967668056949 28.783139025369586 27.569310931856723 19.7178733622042 80-81 24.2180894324704 28.89198130611889 27.279294524841774 19.610634736568937 82-83 24.410906956735364 28.997963039780437 26.995880574564378 19.59524942891982 84-85 24.55665547562564 28.993058309966674 26.850469771344464 19.59981644306322 86-87 24.750903294659736 28.948342889879225 26.775818508466593 19.524935306994447 88-89 24.963890584272672 28.941021877453192 26.641595818197235 19.453491720076904 90-91 25.150819687437785 28.902116332395313 26.5313587784972 19.415705201669706 92-93 25.318905462512763 28.875015480847694 26.486935538738486 19.319143517901054 94-95 25.47320771758742 28.85721020661927 26.38817426016327 19.281407815630036 96-97 25.59044052060035 28.808775194948815 26.326644558033728 19.274139726417104 98-99 25.736555660552902 28.841630129134188 26.241003425110165 19.180810785202752 100 25.799689929343682 28.85037052528733 26.155551849091868 19.194387696277108 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32026.0 1 22961.5 2 11736.0 3 9039.0 4 8378.0 5 8260.5 6 8303.5 7 8347.0 8 8390.0 9 8525.0 10 9036.0 11 11561.5 12 95228.5 13 116272.5 14 54527.0 15 56817.5 16 64390.0 17 71771.0 18 78433.5 19 85406.5 20 94309.5 21 108997.5 22 131018.5 23 156194.5 24 181770.5 25 210904.5 26 245855.5 27 288687.0 28 341923.5 29 394451.5 30 437187.5 31 477957.0 32 523829.0 33 565753.0 34 601090.5 35 643769.5 36 691709.5 37 735571.5 38 777426.0 39 807905.0 40 822618.0 41 844871.0 42 876343.0 43 887740.0 44 889579.5 45 904253.0 46 919117.5 47 926102.0 48 921133.5 49 909231.0 50 896361.0 51 877692.0 52 852577.5 53 808573.5 54 749616.0 55 692787.5 56 634685.0 57 570443.5 58 501010.5 59 427466.0 60 352370.5 61 283434.5 62 221836.5 63 166300.5 64 122156.5 65 89733.5 66 65768.0 67 48657.0 68 36069.5 69 27431.5 70 21336.5 71 16617.5 72 13329.0 73 10561.0 74 8219.0 75 6758.5 76 5283.0 77 3562.5 78 2389.0 79 1732.5 80 1370.0 81 1100.5 82 838.0 83 665.5 84 547.5 85 461.5 86 403.5 87 331.5 88 287.5 89 245.0 90 203.0 91 180.0 92 168.0 93 145.5 94 117.5 95 102.5 96 83.0 97 64.5 98 51.0 99 41.5 100 26.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3602089279469332 2 0.33565350149852025 3 0.15472550944048974 4 0.15212707151156887 5 0.23073827976640382 6 0.09003154977456389 7 0.11900920922501872 8 0.12221683231238413 9 0.11679057191957253 10-11 0.11277822275870626 12-13 0.11352278239680517 14-15 0.1190806568670585 16-17 0.12237100880310159 18-19 0.13109326173527522 20-21 0.11582226834982269 22-23 0.09419995562725389 24-25 0.07897220686724653 26-27 0.0738731014669329 28-29 0.0833023100150792 30-31 0.08569768622451857 32-33 0.08661898476661063 34-35 0.08689913473145087 36-37 0.083700912649617 38-39 0.0829337905982424 40-41 0.0837178344595738 42-43 0.052265830353214575 44-45 0.05471761259584325 46-47 0.07020482910852145 48-49 0.07472483256809148 50-51 0.08260287520353177 52-53 0.08687281191596254 54-55 0.0891610166623422 56-57 0.08896735594839224 58-59 0.0882848429468016 60-61 0.0867205156263514 62-63 0.09037562657701868 64-65 0.09595982386276035 66-67 0.10295793238044743 68-69 0.10698532315016414 70-71 0.10604334239590267 72-73 0.0955480598204784 74-75 0.09519458201249206 76-77 0.09740757871461932 78-79 0.0968397579805136 80-81 0.10229046098770725 82-83 0.09171244956360532 84-85 0.09651636339022822 86-87 0.10969281274325102 88-89 0.11497805805308935 90-91 0.12049832850121649 92-93 0.11978573228192488 94-95 0.11266729089343397 96-97 0.10930925171756371 98-99 0.10606966521139101 100 0.10039145787033381 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.65929E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.64840883486379 #Duplication Level Percentage of deduplicated Percentage of total 1 66.88121565247673 26.517337815620877 2 16.717227662069917 13.256229538624842 3 6.2793299750378635 7.4689632617794866 4 2.9109639200327773 4.616603504199891 5 1.600256891355108 3.1723819734627763 6 1.1082035804301167 2.636310517749187 7 0.7632002474854153 2.1181772804619676 8 0.5677890927602678 1.8009547265388393 9 0.4216249271753443 1.5045081738855948 >10 2.406120040558371 17.15788445467333 >50 0.20076796513475623 5.613697573005823 >100 0.14218090246895107 8.745965104962695 >500 4.926958416620624E-4 0.12539400536398898 >1k 5.791525810458235E-4 0.41245761856031615 >5k 9.45891833279313E-6 0.03456286259965472 >10k+ 3.783567333117252E-5 4.8185715885108475 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1169803 4.398929789530288 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 57939 0.21787394379702854 Illumina Single End PCR Primer 1 (100% over 50bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 30891 0.11616258475006487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.25342478631514426 0.0 0.0 7.520804425241323E-6 2.6322815488344634E-5 2 0.27063990764452167 3.7604022126206617E-6 0.0 7.520804425241323E-6 2.6322815488344634E-5 3 0.2892313361837182 3.7604022126206617E-6 0.0 7.520804425241323E-6 2.6322815488344634E-5 4 0.29369493361009896 1.1281206637861986E-5 0.0 7.520804425241323E-6 3.7604022126206616E-5 5 0.2942928375619056 1.5041608850482647E-5 0.0 7.520804425241323E-6 3.7604022126206616E-5 6 0.294616232152191 2.256241327572397E-5 0.0 1.1281206637861986E-5 4.8885228764068605E-5 7 0.29508628242876855 4.512482655144794E-5 0.0 1.5041608850482647E-5 4.8885228764068605E-5 8 0.29735004456076625 5.264563097668927E-5 0.0 1.5041608850482647E-5 4.8885228764068605E-5 9 0.2980720417855894 5.640603318930993E-5 0.0 1.5041608850482647E-5 4.8885228764068605E-5 10-11 0.298433040398001 5.828623429562026E-5 0.0 1.5041608850482647E-5 4.8885228764068605E-5 12-13 0.2987977994126252 6.016643540193059E-5 0.0 1.5041608850482647E-5 6.580703872086159E-5 14-15 0.2993092141135416 7.520804425241323E-5 0.0 2.068221216941364E-5 7.520804425241323E-5 16-17 0.2997980664011823 8.648925089027522E-5 0.0 2.256241327572397E-5 8.084864757134423E-5 18-19 0.3002963196943545 9.401005531551655E-5 0.0 2.4442614382034303E-5 8.460904978396489E-5 20-21 0.3006479173012345 1.090516641659992E-4 0.0 2.8203016594654966E-5 9.024965310289588E-5 22-23 0.30103335852802815 1.2785367522910251E-4 0.0 3.0083217700965294E-5 9.024965310289588E-5 24-25 0.30198097988560857 1.3537447965434384E-4 0.0 3.196341880727563E-5 9.401005531551655E-5 26-27 0.3046997506853333 1.3725468076065419E-4 0.0 3.7604022126206616E-5 9.965065863444754E-5 28-29 0.31217543028402317 1.5041608850482646E-4 0.0 4.136442433882728E-5 1.0153085974075787E-4 30-31 0.3288246110804012 1.579368929300678E-4 0.0 4.136442433882728E-5 1.0529126195337854E-4 32-33 0.35965802902278426 1.6921809956792978E-4 1.8802011063103309E-6 4.136442433882728E-5 1.090516641659992E-4 34-35 0.41346562428317335 1.767389039931711E-4 3.7604022126206617E-6 4.512482655144794E-5 1.090516641659992E-4 36-37 0.49688450676684376 1.8425970841841243E-4 7.520804425241323E-6 4.512482655144794E-5 1.090516641659992E-4 38-39 0.6190223706327629 1.880201106310331E-4 7.520804425241323E-6 4.512482655144794E-5 1.1657246859124053E-4 40-41 0.777470678263747 1.880201106310331E-4 7.520804425241323E-6 4.512482655144794E-5 1.222130719101715E-4 42-43 0.9689353173215407 2.0118151837520543E-4 7.520804425241323E-6 4.512482655144794E-5 1.2597347412279217E-4 44-45 1.2044361464902287 2.0306171948151575E-4 7.520804425241323E-6 4.512482655144794E-5 1.3725468076065419E-4 46-47 1.4918380469975068 2.1434292611937774E-4 7.520804425241323E-6 5.264563097668927E-5 1.5041608850482646E-4 48-49 1.8350123529212685 2.237439316509294E-4 7.520804425241323E-6 5.264563097668927E-5 1.579368929300678E-4 50-51 2.2323120080923857 2.3126473607617073E-4 7.520804425241323E-6 6.204663650824093E-5 1.6169729514268845E-4 52-53 2.6629551496828103 2.3690533939510169E-4 7.520804425241323E-6 6.392683761455126E-5 1.767389039931711E-4 54-55 3.139187527497941 2.4066574160772235E-4 7.520804425241323E-6 7.520804425241323E-5 1.8049930620579177E-4 56-57 3.670618849392131 2.50066747139274E-4 7.520804425241323E-6 7.89684464650339E-5 1.880201106310331E-4 58-59 4.2546976824641165 2.5946775267082566E-4 7.520804425241323E-6 7.89684464650339E-5 1.936607139499641E-4 60-61 4.886904023254328 2.6322815488344635E-4 1.1281206637861986E-5 8.084864757134423E-5 2.0118151837520543E-4 62-63 5.54624542641081 2.688687582023773E-4 1.1281206637861986E-5 8.648925089027522E-5 2.068221216941364E-4 64-65 6.241829586092528 2.763895626276187E-4 1.1281206637861986E-5 8.648925089027522E-5 2.068221216941364E-4 66-67 6.974448066965243 2.8955097037179097E-4 1.1281206637861986E-5 8.836945199658555E-5 2.124627250130674E-4 68-69 7.745950986917561 2.95191573690722E-4 1.1281206637861986E-5 9.024965310289588E-5 2.293845349698604E-4 70-71 8.554702570986993 2.970717747970323E-4 1.1281206637861986E-5 9.024965310289588E-5 2.425459427140327E-4 72-73 9.378339707215083 3.083529814348943E-4 1.1281206637861986E-5 9.024965310289588E-5 2.5570735045820503E-4 74-75 10.219455192927436 3.1211338364751495E-4 1.1281206637861986E-5 9.401005531551655E-5 2.5946775267082566E-4 76-77 11.087538403107597 3.252747913916873E-4 1.1281206637861986E-5 9.777045752813721E-5 2.707489593086877E-4 78-79 11.978168984954632 3.459570035611009E-4 1.1281206637861986E-5 1.0153085974075787E-4 2.801499648402393E-4 80-81 12.89131121464752 3.6099861241158354E-4 1.1281206637861986E-5 1.1281206637861986E-4 3.0835298143489426E-4 82-83 13.814061271993651 3.760402212620662E-4 1.1281206637861986E-5 1.1845266969755084E-4 3.271549924979976E-4 84-85 14.74087068352831 3.816808245809972E-4 1.1281206637861986E-5 1.2033287080386117E-4 3.4031640024216993E-4 86-87 15.678312632319152 3.948422323251695E-4 1.1281206637861986E-5 1.2785367522910251E-4 3.515976068800319E-4 88 16.38575710057948 4.136442433882728E-4 1.1281206637861986E-5 1.2785367522910251E-4 3.6475901462420423E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 151060 0.0 84.26409 8 AGTGGTA 153025 0.0 83.459595 5 GTGGTAT 154365 0.0 82.68628 6 GTATCAA 154315 0.0 82.39205 9 TGGTATC 154290 0.0 82.33602 7 AAGCAGT 160880 0.0 79.84519 1 GCAGTGG 161810 0.0 79.408035 3 CAGTGGT 163445 0.0 78.11893 4 AGCAGTG 168800 0.0 76.10754 2 CAACGCA 148220 0.0 44.44086 12-13 ATCAACG 149560 0.0 44.111645 10-11 ACGCAGA 151095 0.0 43.625515 14-15 TCAACGC 148335 0.0 43.007366 12-13 AGAGTAC 151370 0.0 42.982414 18-19 AACGCAG 150060 0.0 42.609116 14-15 GAGTACT 147700 0.0 42.365154 20-21 CGCAGAG 152065 0.0 41.961758 16-17 TATCAAC 153820 0.0 41.330154 10-11 AGTACTT 156760 0.0 41.19857 20-21 GTACTTT 157485 0.0 39.698013 22-23 >>END_MODULE