FastQCFastQC Report
Thu 26 May 2016
SRR1660742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26592900
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11724194.408767001718504No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGAATCATCTCGTA566460.21301174373611TruSeq Adapter, Index 25 (97% over 38bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT333320.1253417265510719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1589500.083.694268
AGTGGTA1600350.082.7891165
GTGGTAT1611250.082.150446
TGGTATC1621900.082.109027
GTATCAA1619650.082.07829
AAGCAGT1602950.081.001861
GCAGTGG1654850.080.087063
CAGTGGT1708850.077.393154
AGCAGTG1714700.077.064332
CAACGCA1555850.044.2609712-13
ATCAACG1569900.043.9052710-11
ACGCAGA1586050.043.5084814-15
AGAGTAC1606050.042.82542418-19
TCAACGC1555800.042.67653312-13
AACGCAG1573000.042.3143314-15
GAGTACT1563850.042.1643820-21
CGCAGAG1598050.041.6703116-17
AGTACTT1650600.041.36219420-21
TATCAAC1609600.041.27348710-11
GTACTTT1663750.039.6555122-23
Produced by FastQC (version 0.11.5)