##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660742_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26592900 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.452385975203907 34.0 31.0 34.0 28.0 34.0 2 32.03436293145915 34.0 31.0 34.0 30.0 34.0 3 31.858448871691316 34.0 31.0 34.0 30.0 34.0 4 35.693458554727016 37.0 35.0 37.0 35.0 37.0 5 35.957592740919566 37.0 35.0 37.0 35.0 37.0 6 35.51481297639596 37.0 35.0 37.0 35.0 37.0 7 35.68468485949257 37.0 35.0 37.0 35.0 37.0 8 35.98557351774346 37.0 35.0 37.0 35.0 37.0 9 37.79349510583652 39.0 38.0 39.0 35.0 39.0 10-11 37.80032252969778 39.0 37.5 39.0 35.0 39.0 12-13 37.85999435939668 39.0 38.0 39.0 35.0 39.0 14-15 39.31337896581418 41.0 39.0 41.0 36.0 41.0 16-17 39.28469762605808 41.0 39.0 41.0 36.0 41.0 18-19 39.28561123457765 41.0 39.0 41.0 36.0 41.0 20-21 39.23568946974568 40.5 39.0 41.0 36.0 41.0 22-23 39.125264581899685 40.0 39.0 41.0 35.5 41.0 24-25 39.13182298658665 40.0 39.0 41.0 36.0 41.0 26-27 39.07406614171452 40.0 39.0 41.0 35.5 41.0 28-29 38.99821882908596 40.0 39.0 41.0 35.0 41.0 30-31 38.902851964246096 40.0 38.5 41.0 35.0 41.0 32-33 38.78487718526374 40.0 38.0 41.0 35.0 41.0 34-35 38.695291750805666 40.0 38.0 41.0 35.0 41.0 36-37 38.573459532431585 40.0 38.0 41.0 34.5 41.0 38-39 38.04541140680408 40.0 37.5 41.0 33.5 41.0 40-41 37.930557423221984 40.0 37.0 41.0 33.0 41.0 42-43 37.91947269759973 40.0 37.0 41.0 33.0 41.0 44-45 37.80530468658928 40.0 37.0 41.0 33.0 41.0 46-47 37.664965742735845 40.0 36.0 41.0 33.0 41.0 48-49 37.67901836580441 40.0 36.0 41.0 33.0 41.0 50-51 37.65443661654051 40.0 36.0 41.0 33.0 41.0 52-53 37.421437244527674 40.0 36.0 41.0 32.5 41.0 54-55 36.96633501799353 40.0 36.0 41.0 31.0 41.0 56-57 36.541627050829355 39.0 35.0 41.0 30.0 41.0 58-59 36.43332558690477 39.0 35.0 41.0 30.0 41.0 60-61 36.302334194465445 39.0 35.0 41.0 29.5 41.0 62-63 36.077418446277015 39.0 35.0 41.0 29.0 41.0 64-65 35.82097939675627 38.5 35.0 40.5 29.0 41.0 66-67 35.533310977742175 37.5 35.0 40.0 29.0 41.0 68-69 35.25970349228554 37.0 35.0 40.0 29.0 41.0 70-71 34.81620120408079 36.5 34.5 39.0 27.5 41.0 72-73 34.43692427302024 36.0 34.0 39.0 26.5 41.0 74-75 34.158582121543716 35.5 34.0 38.5 27.0 40.0 76-77 32.48249109724776 34.5 32.0 36.5 25.0 39.0 78-79 33.40915955010548 35.0 34.0 37.0 26.0 39.0 80-81 33.30892484460138 35.0 34.0 36.5 26.0 39.0 82-83 33.056841186933354 35.0 34.0 36.0 26.0 38.0 84-85 32.815748639674496 35.0 34.0 36.0 26.0 37.0 86-87 32.607431269248565 35.0 34.0 35.0 26.0 37.0 88-89 32.43800512542822 35.0 34.0 35.0 25.0 36.0 90-91 32.28459564395008 35.0 33.0 35.0 25.0 36.0 92-93 32.14859721955861 35.0 33.0 35.0 25.0 36.0 94-95 32.04608314625332 35.0 33.0 35.0 25.0 36.0 96-97 31.947971526234447 35.0 33.0 35.0 24.0 35.0 98-99 31.861966257910947 35.0 33.0 35.0 24.0 35.0 100 31.810766445178977 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3223669655104544 1101 2 0.9975352260015811 1101 3 1.2987223574298241 1101 4 0.7993171783790061 1101 5 0.49652058921292763 1101 6 0.9358350293328002 1101 7 0.6889029222120655 1101 8 0.4246371109889395 1101 9 0.45111894295902033 1101 10-11 0.3937551470092302 1101 12-13 0.3569095967354201 1101 14-15 0.47636958461961143 1101 16-17 0.49411404641050183 1101 18-19 0.4750963799455761 1101 20-21 0.46655514161481193 1101 22-23 0.43753751571361477 1101 24-25 0.44482751911674967 1101 26-27 0.4604364326201136 1101 28-29 0.46326486731536676 1101 30-31 0.4568837194663118 1101 32-33 0.49957596897643697 1101 34-35 0.4932307696657148 1101 36-37 0.43079021902815384 1101 38-39 0.6610419026130714 1101 40-41 0.6845348960305699 1101 42-43 0.5042554404303061 1101 44-45 0.4713087159014222 1101 46-47 0.5023481586788847 1101 48-49 0.43836666427092297 1101 50-51 0.4289159309160553 1101 52-53 0.4119884712110249 1101 54-55 0.41150536888829237 1101 56-57 0.4333145257341968 1101 58-59 0.4617517659083674 1101 60-61 0.4899042722363589 1101 62-63 0.47382773938068823 1101 64-65 0.41980793283526197 1101 66-67 0.43468471525675767 1101 68-69 0.42936884207159665 1101 70-71 0.5617246912058107 1101 72-73 0.5621045004448817 1101 74-75 0.44982707304749425 1101 76-77 0.3445164363879911 1101 78-79 0.4129431209350969 1101 80-81 0.4078631298844613 1101 82-83 0.40968492438063464 1101 84-85 0.37467330199662996 1101 86-87 0.36445451242571636 1101 88-89 0.36010039419994655 1101 90-91 0.3408576895728572 1101 92-93 0.35484991899962637 1101 94-95 0.30571332379580696 1101 96-97 0.30469138430499854 1101 98-99 0.3324289436271002 1101 100 0.3208087118522691 1102 1 1.262912090576485 1102 2 0.9609951711764566 1102 3 1.265922507041381 1102 4 0.7674645230124 1102 5 0.4238205597956366 1102 6 0.8845614712583725 1102 7 0.6468308943482697 1102 8 0.36913746929519675 1102 9 0.3999889459023578 1102 10-11 0.334746219233395 1102 12-13 0.3316179523820324 1102 14-15 0.41935378794833156 1102 16-17 0.43674547596418734 1102 18-19 0.39798058778152523 1102 20-21 0.38394059947034265 1102 22-23 0.38678705087033904 1102 24-25 0.36336862556830596 1102 26-27 0.3847041465413952 1102 28-29 0.3921283437779479 1102 30-31 0.38966555398776137 1102 32-33 0.3803991656503527 1102 34-35 0.3822810966300807 1102 36-37 0.33428001612640657 1102 38-39 0.5330224032440185 1102 40-41 0.5211700295369468 1102 42-43 0.40502010008665934 1102 44-45 0.36088879076399394 1102 46-47 0.39740567511306324 1102 48-49 0.3796416206901938 1102 50-51 0.3406240145760293 1102 52-53 0.37225913753597695 1102 54-55 0.41780226664380393 1102 56-57 0.4381084752811475 1102 58-59 0.46144171553827107 1102 60-61 0.4513805484428275 1102 62-63 0.4042508393312687 1102 64-65 0.41276062031239746 1102 66-67 0.39269296572464185 1102 68-69 0.38895962927892924 1102 70-71 0.5038921251548487 1102 72-73 0.4890478983205355 1102 74-75 0.36361456318726226 1102 76-77 0.12337388724965592 1102 78-79 0.3679242470202766 1102 80-81 0.37773317354816527 1102 82-83 0.3739064790052993 1102 84-85 0.3521071580852677 1102 86-87 0.35309452114640294 1102 88-89 0.3584446229400413 1102 90-91 0.3502391924284396 1102 92-93 0.35805912062355105 1102 94-95 0.3046972200887694 1102 96-97 0.29917178645330367 1102 98-99 0.3387122283752646 1102 100 0.3502327636716167 1103 1 1.2344946733321933 1103 2 0.906715862119519 1103 3 1.1881755755772971 1103 4 0.7438391622364335 1103 5 0.4220781882076068 1103 6 0.8782141329636914 1103 7 0.6546460014688265 1103 8 0.3759248116517213 1103 9 0.39064579249244247 1103 10-11 0.31084971697987385 1103 12-13 0.29195885772330143 1103 14-15 0.3474842474107547 1103 16-17 0.37591741830256353 1103 18-19 0.3306520027343893 1103 20-21 0.33896205154275805 1103 22-23 0.3187318459739359 1103 24-25 0.34694323474192146 1103 26-27 0.3361390143725629 1103 28-29 0.35340514327200623 1103 30-31 0.3261745432933978 1103 32-33 0.32680130874550173 1103 34-35 0.3555782272815051 1103 36-37 0.31287884827921175 1103 38-39 0.5305595263670568 1103 40-41 0.5532383804749799 1103 42-43 0.4278880220508867 1103 44-45 0.3747130713034821 1103 46-47 0.36798106726608637 1103 48-49 0.33661843173690187 1103 50-51 0.345637213683176 1103 52-53 0.344068560332758 1103 54-55 0.35185686128678384 1103 56-57 0.3294982216407689 1103 58-59 0.3574236940085527 1103 60-61 0.3659306295481315 1103 62-63 0.326984103794679 1103 64-65 0.32531896513135905 1103 66-67 0.3080292037810466 1103 68-69 0.2954712583308421 1103 70-71 0.4542600585815322 1103 72-73 0.4526079298862413 1103 74-75 0.3346905675855396 1103 76-77 0.19780325633601592 1103 78-79 0.3398514380528965 1103 80-81 0.36725216510195935 1103 82-83 0.38554799492318637 1103 84-85 0.36765074813568077 1103 86-87 0.3458294914481357 1103 88-89 0.33977903497151374 1103 90-91 0.3525645048516495 1103 92-93 0.32299974437184886 1103 94-95 0.3372584470566622 1103 96-97 0.3319622962798512 1103 98-99 0.3487993888583816 1103 100 0.36093253394905034 1104 1 1.2011892514057223 1104 2 0.9000189614198604 1104 3 1.195020044446352 1104 4 0.7413204175372243 1104 5 0.4208033427008644 1104 6 0.8510286151847097 1104 7 0.6431731155543687 1104 8 0.3712440520207707 1104 9 0.37761568247663746 1104 10-11 0.2888026094182834 1104 12-13 0.26606231220870313 1104 14-15 0.3283614012933569 1104 16-17 0.35653383513616177 1104 18-19 0.3225805707783209 1104 20-21 0.3191037546984674 1104 22-23 0.2951181347423315 1104 24-25 0.3203293574891717 1104 26-27 0.32601420661718805 1104 28-29 0.3006144171744083 1104 30-31 0.3181880406179616 1104 32-33 0.35220178953885295 1104 34-35 0.36039267185900314 1104 36-37 0.3631868397760911 1104 38-39 0.5217963529611751 1104 40-41 0.5059853416304136 1104 42-43 0.3674654008135221 1104 44-45 0.31720263392403325 1104 46-47 0.3590170141846798 1104 48-49 0.28597214257746373 1104 50-51 0.26797911315576783 1104 52-53 0.2898219536596187 1104 54-55 0.25353429590238363 1104 56-57 0.2785267108977152 1104 58-59 0.312115110026312 1104 60-61 0.3290531123116054 1104 62-63 0.2584839883244001 1104 64-65 0.2510670048676076 1104 66-67 0.27093439784244566 1104 68-69 0.23492958510902895 1104 70-71 0.3973679438694049 1104 72-73 0.4059240457734603 1104 74-75 0.29476348503427374 1104 76-77 0.1781045861125392 1104 78-79 0.2762929897369375 1104 80-81 0.28070256224806656 1104 82-83 0.3062467059909295 1104 84-85 0.2920700237616174 1104 86-87 0.3105748309401548 1104 88-89 0.3073158903096953 1104 90-91 0.30322536608949235 1104 92-93 0.31735781204988456 1104 94-95 0.31405975738518777 1104 96-97 0.32175829100919984 1104 98-99 0.3143072410116119 1104 100 0.32100123998536034 1105 1 1.0995808950329362 1105 2 0.8164187222929158 1105 3 1.111445185857086 1105 4 0.7005098665922205 1105 5 0.36811862003177254 1105 6 0.8245191331578425 1105 7 0.5931224861154121 1105 8 0.30798853480642663 1105 9 0.2998087569605232 1105 10-11 0.23182090303415492 1105 12-13 0.22663296198632565 1105 14-15 0.28908903316578716 1105 16-17 0.26290943382164755 1105 18-19 0.23064414300755942 1105 20-21 0.2614706037062078 1105 22-23 0.25644848143353727 1105 24-25 0.252315500928205 1105 26-27 0.27460377223589916 1105 28-29 0.26314126670636284 1105 30-31 0.2618739132186505 1105 32-33 0.2719364632524872 1105 34-35 0.252029092304177 1105 36-37 0.2520348338359213 1105 38-39 0.4960898019791813 1105 40-41 0.4714769714319118 1105 42-43 0.30958903858174125 1105 44-45 0.2995967818269847 1105 46-47 0.3165481395007248 1105 48-49 0.2510895748042117 1105 50-51 0.28290839577344684 1105 52-53 0.2847280897975608 1105 54-55 0.3437478859339862 1105 56-57 0.3383853107929937 1105 58-59 0.2977684676746435 1105 60-61 0.2614349163829246 1105 62-63 0.2282659170780903 1105 64-65 0.22251004814944508 1105 66-67 0.2325453846090184 1105 68-69 0.22823788622240926 1105 70-71 0.38395733205131677 1105 72-73 0.39956376992294196 1105 74-75 0.25956352841722463 1105 76-77 0.10977789286348383 1105 78-79 0.22627587894879042 1105 80-81 0.2873542287510915 1105 82-83 0.28696968993081384 1105 84-85 0.27151464144087356 1105 86-87 0.2845046173491852 1105 88-89 0.2761346166345504 1105 90-91 0.26695468506085973 1105 92-93 0.2631521473667746 1105 94-95 0.2533122818100466 1105 96-97 0.24657574567445906 1105 98-99 0.2767165872136985 1105 100 0.24235323399264885 1106 1 1.1047745927622188 1106 2 0.8022151527699606 1106 3 1.1014799939160653 1106 4 0.6487644427614683 1106 5 0.3284318130259507 1106 6 0.7717467940200109 1106 7 0.5555304352012129 1106 8 0.2760869678830531 1106 9 0.2708351203354482 1106 10-11 0.1974373031479999 1106 12-13 0.16608741172382224 1106 14-15 0.20848848149194765 1106 16-17 0.1981533605826158 1106 18-19 0.18114980057773522 1106 20-21 0.22295499385618456 1106 22-23 0.23329755115795336 1106 24-25 0.21118577086210877 1106 26-27 0.1899820466164428 1106 28-29 0.18095228119896234 1106 30-31 0.1877684025930293 1106 32-33 0.21278197619751893 1106 34-35 0.23291382659760274 1106 36-37 0.22422820533687826 1106 38-39 0.4340728420326414 1106 40-41 0.4190223115803846 1106 42-43 0.3169394950811082 1106 44-45 0.2616072898432904 1106 46-47 0.2513533684394318 1106 48-49 0.2194459377714395 1106 50-51 0.22899365142643546 1106 52-53 0.23574342436085516 1106 54-55 0.30241639775224627 1106 56-57 0.3106655420705522 1106 58-59 0.31603743334434853 1106 60-61 0.28605332836380626 1106 62-63 0.2591298312523733 1106 64-65 0.22328125478978933 1106 66-67 0.1948862847926236 1106 68-69 0.2241639639345081 1106 70-71 0.33427650222918714 1106 72-73 0.34228661778072933 1106 74-75 0.2487385686475605 1106 76-77 0.11116525250461251 1106 78-79 0.24199044402914183 1106 80-81 0.25264403949399394 1106 82-83 0.25491160305478644 1106 84-85 0.26922572595652383 1106 86-87 0.26825567694549335 1106 88-89 0.24081006127876492 1106 90-91 0.2631378217300906 1106 92-93 0.2667079695669017 1106 94-95 0.25080145079419935 1106 96-97 0.2549925239770836 1106 98-99 0.2647137116584801 1106 100 0.28193692430566486 1107 1 1.0522368542064129 1107 2 0.7359461615222358 1107 3 1.0399299765553565 1107 4 0.6400394842979793 1107 5 0.321501870449886 1107 6 0.7767940066960648 1107 7 0.5261589107073945 1107 8 0.25062480044416446 1107 9 0.2709182726195323 1107 10-11 0.1779866305222768 1107 12-13 0.12798117046656898 1107 14-15 0.1887649835099694 1107 16-17 0.18914793365388505 1107 18-19 0.13519561887020615 1107 20-21 0.1267406029566942 1107 22-23 0.16418141933348096 1107 24-25 0.17130283632700838 1107 26-27 0.14409223255486125 1107 28-29 0.15500737462520675 1107 30-31 0.14316639348154325 1107 32-33 0.15594515772407647 1107 34-35 0.17405518549482224 1107 36-37 0.17475148229925708 1107 38-39 0.3875422772162409 1107 40-41 0.3738910405587106 1107 42-43 0.2466451219132324 1107 44-45 0.20466121840059515 1107 46-47 0.23900172726904856 1107 48-49 0.2354182409954504 1107 50-51 0.2119397122431934 1107 52-53 0.2112023190380441 1107 54-55 0.218071418762122 1107 56-57 0.23525706920400324 1107 58-59 0.21174440907131498 1107 60-61 0.18450858198470144 1107 62-63 0.14654730791982473 1107 64-65 0.13305442462510086 1107 66-67 0.18858309143555374 1107 68-69 0.1919631701566189 1107 70-71 0.33123271316322445 1107 72-73 0.372443246599957 1107 74-75 0.2074721157003907 1107 76-77 0.04238511540739154 1107 78-79 0.20481479895767052 1107 80-81 0.2462985808796745 1107 82-83 0.24658709337169427 1107 84-85 0.24707177715994533 1107 86-87 0.20137736716615962 1107 88-89 0.1974637686279408 1107 90-91 0.20977650194004838 1107 92-93 0.2060887447719537 1107 94-95 0.1714876962929779 1107 96-97 0.20058820224062757 1107 98-99 0.21118101641204134 1107 100 0.2161522632277446 1108 1 1.0254530923234952 1108 2 0.7138377629362154 1108 3 1.010252121460688 1108 4 0.6078478320168657 1108 5 0.2959474524583783 1108 6 0.7266681959534935 1108 7 0.5095733097267754 1108 8 0.22103724713899453 1108 9 0.18208902940983762 1108 10-11 0.11277108086535037 1108 12-13 0.10651557355831187 1108 14-15 0.1325805344545401 1108 16-17 0.11915561034118127 1108 18-19 0.09327061579870843 1108 20-21 0.09946200984108344 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-0.7135479215530012 2316 78-79 -0.9868146660203294 2316 80-81 -1.1512616345834914 2316 82-83 -1.2383396140446052 2316 84-85 -1.0023139825536163 2316 86-87 -0.7737688319729585 2316 88-89 -0.6911029248517409 2316 90-91 -0.7330816349752709 2316 92-93 -0.8286565887399817 2316 94-95 -0.8273392330400924 2316 96-97 -0.8928567933480913 2316 98-99 -1.0455550616593783 2316 100 -0.9518432667626975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 48.0 7 779.0 8 948.0 9 406.0 10 349.0 11 538.0 12 761.0 13 1201.0 14 2805.0 15 6812.0 16 14683.0 17 22638.0 18 29890.0 19 38138.0 20 51293.0 21 72546.0 22 109019.0 23 178070.0 24 299940.0 25 400068.0 26 412064.0 27 357515.0 28 326351.0 29 349347.0 30 407501.0 31 486554.0 32 585958.0 33 747276.0 34 1023864.0 35 1596312.0 36 2915303.0 37 5879871.0 38 7909012.0 39 2360283.0 40 4757.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.849912553808979 32.263514329045115 27.215639199067727 24.670933918078187 2 20.960919135988224 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23.161825653585417 29.542276100148413 26.62632872974325 20.66956951652292 22-23 23.2700666340275 27.007658033081107 29.163313393032087 20.55896193985931 24-25 20.565423162991806 29.530651746933973 26.345077882197472 23.558847207876752 26-27 20.6082339645118 26.766404300248848 31.90700310623793 20.718358629001422 28-29 20.586599880623886 26.972545148119043 31.76487154732866 20.67598342392841 30-31 20.691697878668194 26.764412197918762 31.870998687840846 20.672891235572198 32-33 20.70972082704485 27.02286532220668 31.54314034692498 20.724273503823486 34-35 20.830556757257742 27.03218808748688 31.431442888596596 20.705812266658786 36-37 20.657048642727897 26.963081122582434 31.61788877166175 20.76198146302792 38-39 20.800246861230505 27.08541995698447 31.54268805878714 20.57164512299789 40-41 20.716969615944592 27.1515528606674 31.611641646062953 20.51983587732505 42-43 20.696065127063832 27.171515168701728 31.43240177936829 20.700017924866152 44-45 20.75195362732273 27.12972013955361 31.505499288944634 20.612826944179027 46-47 20.783220430471435 27.233047451587755 31.390942583378145 20.592789534562666 48-49 20.97967402905492 27.260410166692555 31.239527042346758 20.52038876190576 50-51 20.88101043624696 27.477994789014364 31.159258004728585 20.48173677001009 52-53 21.109674288057732 27.469112484809266 30.77709030163251 20.644122925500493 54-55 21.36654744460297 27.661270412089777 30.143910382754115 20.82827176055314 56-57 21.620071402826603 28.153551339482384 29.286217159415507 20.940160098275506 58-59 21.684247417141847 28.623849970909475 28.816375107629668 20.87552750431901 60-61 21.737773382707168 28.871444635989086 28.344085058156576 21.04669692314717 62-63 21.849532142259505 29.033467370920352 28.00953148930442 21.107468997515724 64-65 21.742434647358746 29.119207640076144 28.117908560491696 21.020449152073418 66-67 21.83241059272177 29.132061425003425 27.982060376381114 21.05346760589369 68-69 21.62907127451337 29.199225529937074 28.091797669084745 21.079905526464806 70-71 21.70863828555617 29.091569890174178 28.07671226336207 21.123079560907584 72-73 21.557115369995795 29.047832320238225 28.086427446526812 21.308624863239167 74-75 21.632839606015175 29.041494032352865 27.972713737576278 21.35295262405568 76-77 21.68196951690518 29.133886412016746 27.751004089348896 21.433139981729173 78-79 21.72940770652725 29.260238087610507 27.426392710300167 21.58396149556207 80-81 21.778431229141187 29.380497482834016 27.14651173149989 21.694559556524908 82-83 21.766423460642244 29.474235758574718 26.830107385296188 21.929233395486854 84-85 21.729473225940335 29.477832700237737 26.670004075167274 22.122689998654653 86-87 21.737029946565873 29.48133255692235 26.559352567451832 22.222284929059942 88-89 21.705855847415712 29.506046559717582 26.428816580652743 22.35928101221397 90-91 21.728567586506436 29.397127223751653 26.319656276187036 22.554648913554878 92-93 21.72837875804904 29.374161634238177 26.27515073770543 22.622308870007345 94-95 21.729842850932084 29.3292977273039 26.20858742983924 22.732271991924772 96-97 21.717343448672572 29.235659716371863 26.193331395330897 22.853665439624667 98-99 21.668988024397326 29.19347682959959 26.21300698784823 22.924528158154857 100 21.625559707996455 29.119403281995655 26.17493880804192 23.080098201965974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22586.0 1 17033.5 2 9219.0 3 6459.5 4 5866.0 5 5901.0 6 6088.5 7 6174.0 8 6238.5 9 6535.0 10 7365.0 11 13571.5 12 103437.0 13 121960.0 14 54169.5 15 56372.5 16 64794.0 17 72280.5 18 79553.0 19 87290.0 20 96365.5 21 111606.0 22 134415.0 23 159906.5 24 186240.5 25 215971.5 26 250870.5 27 293934.0 28 347242.5 29 400378.5 30 444224.0 31 484492.5 32 528836.0 33 570860.5 34 605967.5 35 644271.5 36 689248.5 37 734169.0 38 777646.5 39 808356.5 40 824653.5 41 849520.5 42 881469.5 43 894265.0 44 897505.5 45 910010.5 46 920757.0 47 921454.0 48 911473.0 49 901460.5 50 894325.0 51 881475.5 52 858344.5 53 813422.5 54 753071.5 55 694319.0 56 634584.0 57 568010.5 58 496339.0 59 421592.0 60 345622.5 61 276712.0 62 215233.0 63 160047.5 64 116293.5 65 84332.5 66 61466.5 67 45164.0 68 33103.0 69 24810.5 70 18851.0 71 14386.5 72 11325.0 73 8849.0 74 6834.5 75 5390.5 76 3931.0 77 2289.0 78 1279.0 79 819.5 80 514.0 81 328.5 82 226.0 83 157.0 84 90.0 85 52.5 86 36.0 87 29.5 88 22.5 89 13.5 90 10.0 91 6.5 92 4.0 93 4.0 94 3.5 95 1.5 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30307337672837487 2 0.004956210116234032 3 1.9691910246719988 4 6.01664354019306E-4 5 3.760402212620662E-5 6 0.0075696896540053925 7 0.006031685149043542 8 2.3314493718248105E-4 9 0.0 10-11 7.520804425241325E-5 12-13 3.384361991358595E-5 14-15 0.0 16-17 2.8203016594654966E-5 18-19 0.0 20-21 2.5382714935189466E-4 22-23 3.9672243343147985E-4 24-25 4.098838411756522E-4 26-27 0.002874827491548496 28-29 0.004948689311808791 30-31 0.004634695727054966 32-33 0.012371723279521978 34-35 0.00807170334939025 36-37 0.004228572288091934 38-39 0.4989677695926356 40-41 0.006825130015906501 42-43 0.0155605443558243 44-45 0.02789278341211376 46-47 0.026851151999217837 48-49 0.03256320296018862 50-51 0.03479688187448529 52-53 0.0533977114192134 54-55 0.030818376333532634 56-57 0.024132381199493097 58-59 0.019565372712265303 60-61 0.020825107453493225 62-63 0.022859485050521003 64-65 0.038480195841747236 66-67 0.03911758401678644 68-69 0.040809765012465735 70-71 0.1336052104133058 72-73 0.056494402641306514 74-75 0.05248581388265289 76-77 0.05438857740223894 78-79 0.050067875259937805 80-81 0.04891531198176957 82-83 0.045923912021629834 84-85 0.04661394582764573 86-87 0.053371388603725055 88-89 0.05507485080604222 90-91 0.049154097522270984 92-93 0.052767844048599445 94-95 0.05945007878042635 96-97 0.05906087715142011 98-99 0.039095021603510714 100 0.005937675093728025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.65929E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.32235680951516 #Duplication Level Percentage of deduplicated Percentage of total 1 65.86548060099244 25.899859296224243 2 17.40012922373845 13.684281797350309 3 6.346665121211046 7.486974913402969 4 2.964667534079117 4.66310858386578 5 1.7501999125396466 3.4410992724433105 6 1.0742209915621135 2.5344540673485962 7 0.7773384139108535 2.139674493148162 8 0.5936080149509889 1.8673652935112635 9 0.44309457584749645 1.5681170710652435 >10 2.439771448682202 17.30281720247236 >50 0.20610115018157957 5.726002575922682 >100 0.13763302427975097 8.184954035808111 >500 4.5537089019347284E-4 0.1178715651546628 >1k 5.675339840383799E-4 0.4063440724658441 >5k 9.583307151796235E-6 0.02256507280749803 >10k+ 5.7499842909943436E-5 4.954510687009051 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1172419 4.408767001718504 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGAATCATCTCGTA 56646 0.21301174373611 TruSeq Adapter, Index 25 (97% over 38bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 33332 0.1253417265510719 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.24315888827468987 0.0 0.0 0.0 3.7604022126206617E-6 2 0.26024991633105077 0.0 0.0 0.0 7.520804425241323E-6 3 0.2786495643574037 3.7604022126206617E-6 0.0 0.0 7.520804425241323E-6 4 0.2831883698280368 1.1281206637861986E-5 0.0 3.7604022126206617E-6 1.1281206637861986E-5 5 0.28377499257320565 1.5041608850482647E-5 0.0 3.7604022126206617E-6 1.1281206637861986E-5 6 0.28411342877234147 3.0083217700965294E-5 0.0 3.7604022126206617E-6 1.5041608850482647E-5 7 0.28457595824449383 3.7604022126206616E-5 0.0 3.7604022126206617E-6 1.8802011063103308E-5 8 0.2869149284207439 3.7604022126206616E-5 0.0 7.520804425241323E-6 1.8802011063103308E-5 9 0.287667008863268 4.8885228764068605E-5 0.0 7.520804425241323E-6 2.6322815488344634E-5 10-11 0.28802424707346697 5.264563097668927E-5 0.0 7.520804425241323E-6 2.6322815488344634E-5 12-13 0.2883025168372009 5.828623429562026E-5 0.0 7.520804425241323E-6 3.0083217700965297E-5 14-15 0.2885225003666392 6.392683761455126E-5 0.0 9.401005531551654E-6 4.324462544513761E-5 16-17 0.2887537651027154 6.768723982717192E-5 0.0 1.1281206637861986E-5 5.828623429562026E-5 18-19 0.2891335657261901 7.520804425241323E-5 0.0 1.1281206637861986E-5 6.016643540193059E-5 20-21 0.28953216836072787 9.589025642182688E-5 0.0 1.1281206637861986E-5 6.392683761455126E-5 22-23 0.2899382917996909 1.0153085974075787E-4 0.0 1.1281206637861986E-5 7.708824535872356E-5 24-25 0.29089719436390915 1.0529126195337854E-4 0.0 1.3161407744172317E-5 7.89684464650339E-5 26-27 0.2936065641581024 1.1845266969755086E-4 0.0 1.8802011063103308E-5 8.272884867765456E-5 28-29 0.30114052999108787 1.334942785480335E-4 0.0 1.8802011063103308E-5 8.272884867765456E-5 30-31 0.3179100436582697 1.391348818669645E-4 0.0 1.8802011063103308E-5 8.272884867765456E-5 32-33 0.34909317900642656 1.579368929300678E-4 0.0 1.8802011063103308E-5 9.965065863444754E-5 34-35 0.40326177287922715 1.767389039931711E-4 1.8802011063103309E-6 1.8802011063103308E-5 1.090516641659992E-4 36-37 0.4870209717631398 1.8049930620579177E-4 3.7604022126206617E-6 1.8802011063103308E-5 1.1845266969755084E-4 38-39 0.6099748429091976 1.8049930620579177E-4 3.7604022126206617E-6 2.256241327572397E-5 1.2409327301648185E-4 40-41 0.770829807956259 1.8425970841841243E-4 3.7604022126206617E-6 2.256241327572397E-5 1.2785367522910251E-4 42-43 0.9646954638268108 1.9930131726889508E-4 3.7604022126206617E-6 2.256241327572397E-5 1.4289528407958517E-4 44-45 1.2027289238856989 2.0870232280044675E-4 3.7604022126206617E-6 2.4442614382034303E-5 1.5229628961113678E-4 46-47 1.4931748699840934 2.2562413275723972E-4 3.7604022126206617E-6 3.384361991358596E-5 1.579368929300678E-4 48-49 1.8398407093622735 2.481865460329637E-4 3.7604022126206617E-6 3.384361991358596E-5 1.6169729514268845E-4 50-51 2.2410925472588548 2.6134795377713604E-4 3.7604022126206617E-6 3.384361991358596E-5 1.767389039931711E-4 52-53 2.677150668035453 2.6322815488344635E-4 1.1281206637861986E-5 3.948422323251695E-5 1.8425970841841243E-4 54-55 3.158561119697363 2.6510835598975667E-4 1.1281206637861986E-5 4.8885228764068605E-5 1.880201106310331E-4 56-57 3.6957308153680115 2.8391036705285996E-4 1.1281206637861986E-5 5.264563097668927E-5 1.9554091505627442E-4 58-59 4.287153713961246 2.9331137258441166E-4 1.1281206637861986E-5 5.264563097668927E-5 2.0306171948151575E-4 60-61 4.924429076934069 3.0271237811596325E-4 1.1281206637861986E-5 5.640603318930993E-5 2.068221216941364E-4 62-63 5.58823971812025 3.1211338364751495E-4 1.1281206637861986E-5 5.828623429562026E-5 2.181033283319984E-4 64-65 6.287418822317235 3.1211338364751495E-4 1.1281206637861986E-5 7.144764203979258E-5 2.2750433386355004E-4 66-67 7.024940867675206 3.4031640024216993E-4 1.1281206637861986E-5 7.332784314610291E-5 2.3314493718248105E-4 68-69 7.801106686371174 3.4219660134848025E-4 1.1281206637861986E-5 7.708824535872356E-5 2.3314493718248105E-4 70-71 8.616875180969355 3.515976068800319E-4 1.8802011063103308E-5 7.89684464650339E-5 2.4066574160772235E-4 72-73 9.451624305735741 3.6099861241158354E-4 1.8802011063103308E-5 7.89684464650339E-5 2.4066574160772235E-4 74-75 10.306469019926372 3.7227981904944555E-4 1.8802011063103308E-5 8.272884867765456E-5 2.5382714935189466E-4 76-77 11.188841758514492 3.835610256873075E-4 1.8802011063103308E-5 8.648925089027522E-5 2.5570735045820503E-4 78-79 12.092838690026284 4.042432378567212E-4 2.068221216941364E-5 8.648925089027522E-5 2.5570735045820503E-4 80-81 13.01956349251116 4.1552444449458314E-4 2.256241327572397E-5 8.648925089027522E-5 2.6134795377713604E-4 82-83 13.955762628370731 4.268056511324451E-4 2.256241327572397E-5 8.836945199658555E-5 2.745093615213083E-4 84-85 14.897134197473761 4.343264555576865E-4 2.256241327572397E-5 9.024965310289588E-5 2.8203016594654964E-4 86-87 15.849645958131681 4.474878633018588E-4 2.256241327572397E-5 9.024965310289588E-5 2.8203016594654964E-4 88 16.56883980310534 4.5124826551447945E-4 2.6322815488344634E-5 9.024965310289588E-5 2.8579056815917033E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 158950 0.0 83.69426 8 AGTGGTA 160035 0.0 82.789116 5 GTGGTAT 161125 0.0 82.15044 6 TGGTATC 162190 0.0 82.10902 7 GTATCAA 161965 0.0 82.0782 9 AAGCAGT 160295 0.0 81.00186 1 GCAGTGG 165485 0.0 80.08706 3 CAGTGGT 170885 0.0 77.39315 4 AGCAGTG 171470 0.0 77.06433 2 CAACGCA 155585 0.0 44.26097 12-13 ATCAACG 156990 0.0 43.90527 10-11 ACGCAGA 158605 0.0 43.50848 14-15 AGAGTAC 160605 0.0 42.825424 18-19 TCAACGC 155580 0.0 42.676533 12-13 AACGCAG 157300 0.0 42.31433 14-15 GAGTACT 156385 0.0 42.16438 20-21 CGCAGAG 159805 0.0 41.67031 16-17 AGTACTT 165060 0.0 41.362194 20-21 TATCAAC 160960 0.0 41.273487 10-11 GTACTTT 166375 0.0 39.65551 22-23 >>END_MODULE