FastQCFastQC Report
Thu 26 May 2016
SRR1660741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28792991
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13589704.719794480538684No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT350410.1216997567220439No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292170.10147261185890692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1749200.084.561378
AGTGGTA1774900.083.6658255
TGGTATC1774250.083.200157
GTGGTAT1783100.083.189596
GTATCAA1781500.082.939029
AAGCAGT1842150.081.054191
GCAGTGG1863000.080.179843
CAGTGGT1891950.078.36384
AGCAGTG1932600.077.3628542
CAACGCA1722350.044.44606812-13
ATCAACG1736600.044.182410-11
ACGCAGA1749000.043.79401414-15
AGAGTAC1746000.043.1839418-19
TCAACGC1720550.043.0472312-13
AACGCAG1737600.042.7122514-15
GAGTACT1699850.042.61697820-21
CGCAGAG1765050.041.977516-17
AGTACTT1795950.041.6464920-21
TATCAAC1781450.041.439510-11
GTACTTT1813250.039.93526522-23
Produced by FastQC (version 0.11.5)