##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660741_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28792991 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836844946049546 34.0 31.0 34.0 30.0 34.0 2 31.95838383028703 34.0 31.0 34.0 30.0 34.0 3 32.01941205066191 34.0 31.0 34.0 30.0 34.0 4 35.424530504663444 37.0 35.0 37.0 33.0 37.0 5 35.39554619386364 37.0 35.0 37.0 33.0 37.0 6 35.46775689264099 37.0 35.0 37.0 33.0 37.0 7 35.4299833594919 37.0 35.0 37.0 33.0 37.0 8 35.431913551461186 37.0 35.0 37.0 33.0 37.0 9 37.108381654410266 39.0 37.0 39.0 33.0 39.0 10-11 37.05729317944079 39.0 37.0 39.0 33.0 39.0 12-13 37.066299867908825 39.0 37.0 39.0 33.0 39.0 14-15 38.43297000648526 40.0 38.0 41.0 33.0 41.0 16-17 38.41194030519441 40.0 38.0 41.0 33.0 41.0 18-19 38.37889339110341 40.0 38.0 41.0 33.5 41.0 20-21 38.264873958387994 40.0 38.0 41.0 33.5 41.0 22-23 38.101867221783245 40.0 38.0 41.0 33.0 41.0 24-25 38.075877841242686 40.0 38.0 41.0 33.0 41.0 26-27 38.04621027388228 40.0 38.0 41.0 33.0 41.0 28-29 37.95560429967141 40.0 38.0 41.0 33.0 41.0 30-31 37.86051865886389 40.0 38.0 41.0 32.0 41.0 32-33 37.73158742000788 40.0 38.0 41.0 32.0 41.0 34-35 37.57079399983142 40.0 37.5 41.0 31.5 41.0 36-37 37.38412084385398 40.0 37.0 41.0 31.0 41.0 38-39 36.99574384265948 40.0 35.5 41.0 30.5 41.0 40-41 36.932611308078414 39.5 35.0 41.0 30.0 41.0 42-43 36.94396775242975 40.0 35.0 41.0 30.0 41.0 44-45 36.94406900971143 40.0 35.0 41.0 30.0 41.0 46-47 37.039269261744984 40.0 35.0 41.0 31.0 41.0 48-49 36.93320869304617 40.0 35.0 41.0 30.0 41.0 50-51 35.568093603057775 38.5 34.0 40.0 28.0 40.5 52-53 35.7459124514018 38.5 35.0 39.5 28.5 40.5 54-55 36.19816463319146 39.0 35.0 41.0 27.5 41.0 56-57 35.88408119878897 39.0 35.0 41.0 26.0 41.0 58-59 35.70328162503159 39.0 35.0 41.0 26.0 41.0 60-61 35.472701550873964 39.0 35.0 41.0 26.0 41.0 62-63 35.235235408506185 38.5 35.0 41.0 25.5 41.0 64-65 34.97060437382139 38.0 34.5 40.5 25.0 41.0 66-67 34.68804798709519 37.0 34.0 40.0 25.0 41.0 68-69 34.396486683165364 37.0 34.0 40.0 24.5 41.0 70-71 34.08241114999133 36.0 34.0 39.0 24.0 41.0 72-73 33.73575214884761 35.5 33.5 39.0 24.0 41.0 74-75 33.353776323550406 35.0 33.0 38.5 23.5 40.0 76-77 32.960247130977116 35.0 33.0 37.0 22.5 39.5 78-79 32.59269799375828 35.0 33.0 37.0 21.5 39.0 80-81 32.274677941586546 35.0 33.0 36.0 20.0 39.0 82-83 31.964169196593716 35.0 32.0 36.0 20.0 37.5 84-85 31.68095287495488 35.0 32.0 35.5 20.0 37.0 86-87 31.46342800926795 35.0 32.0 35.0 19.0 37.0 88-89 31.25465964963487 35.0 32.0 35.0 19.0 36.0 90-91 31.07981043025367 35.0 31.0 35.0 18.5 36.0 92-93 30.925937722135224 35.0 31.0 35.0 18.0 36.0 94-95 30.744019751195697 35.0 31.0 35.0 17.5 35.5 96-97 30.605413553597124 35.0 31.0 35.0 17.0 35.0 98-99 30.49813447654674 34.5 31.0 35.0 17.0 35.0 100 30.41065348160599 34.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5333335145550429 1101 2 0.5608285009644369 1101 3 0.5476895803687825 1101 4 0.488052300677424 1101 5 0.4934454964037158 1101 6 0.4649290260412329 1101 7 0.4780715761304464 1101 8 0.5053227268023477 1101 9 0.5778573980196882 1101 10-11 0.5863671031759523 1101 12-13 0.563405967812777 1101 14-15 0.6969357856007292 1101 16-17 0.6584225443998264 1101 18-19 0.6723605764245448 1101 20-21 0.7077147777807724 1101 22-23 0.7506832163234876 1101 24-25 0.7452832683264106 1101 26-27 0.6986185218804053 1101 28-29 0.7101127505286442 1101 30-31 0.6748154880887967 1101 32-33 0.6478731425250501 1101 34-35 0.6795960959442127 1101 36-37 0.6545138039460809 1101 38-39 0.6210959468945063 1101 40-41 0.6396826459289784 1101 42-43 0.6306753133640228 1101 44-45 0.6515212586621857 1101 46-47 0.6571854326777782 1101 48-49 0.719700868733284 1101 50-51 0.6517785321797476 1101 52-53 0.634605872561103 1101 54-55 0.6618594741498569 1101 56-57 0.6724505538900658 1101 58-59 0.5830018437847073 1101 60-61 0.5690232586853128 1101 62-63 0.518447092797949 1101 64-65 0.495134594682888 1101 66-67 0.4856556973464663 1101 68-69 0.5037686656956808 1101 70-71 0.5137891682742364 1101 72-73 0.48641310084585143 1101 74-75 0.43945585135686116 1101 76-77 0.44690489289975943 1101 78-79 0.4306864892248896 1101 80-81 0.37068598579529777 1101 82-83 0.40828776393364663 1101 84-85 0.36794195094677207 1101 86-87 0.29211914373539116 1101 88-89 0.2828935802269186 1101 90-91 0.27656735411809663 1101 92-93 0.2797361777165328 1101 94-95 0.3244155869441592 1101 96-97 0.28158405309224577 1101 98-99 0.2435994591633026 1101 100 0.2389376829657195 1102 1 0.44638497910083075 1102 2 0.47833594449732075 1102 3 0.47676028757660305 1102 4 0.4127492394633876 1102 5 0.40444383276263807 1102 6 0.3795781902083135 1102 7 0.3924680800488005 1102 8 0.4012695230256824 1102 9 0.4767314958826674 1102 10-11 0.4597111608126383 1102 12-13 0.47457416830035726 1102 14-15 0.5661514988045013 1102 16-17 0.5570233877850725 1102 18-19 0.5321124662029035 1102 20-21 0.5443039988345006 1102 22-23 0.5894379787805306 1102 24-25 0.5583760411027967 1102 26-27 0.5622756963042761 1102 28-29 0.5571758437938215 1102 30-31 0.5393428069490795 1102 32-33 0.5381371226129943 1102 34-35 0.5336173304241854 1102 36-37 0.5118468865554888 1102 38-39 0.533159510599738 1102 40-41 0.5063411614064606 1102 42-43 0.5053644902216732 1102 44-45 0.5189328009590071 1102 46-47 0.5431173817595294 1102 48-49 0.5886607610231351 1102 50-51 0.5104228649872837 1102 52-53 0.5469011230062222 1102 54-55 0.5595953898597728 1102 56-57 0.5591105971332908 1102 58-59 0.45266273501776766 1102 60-61 0.47381125723367035 1102 62-63 0.4554772399545257 1102 64-65 0.4234513235447892 1102 66-67 0.45517427547032696 1102 68-69 0.39679688038828687 1102 70-71 0.41059082129547875 1102 72-73 0.3639047763191243 1102 74-75 0.3279433978702073 1102 76-77 0.34784965232389453 1102 78-79 0.3209377534577058 1102 80-81 0.3176186367443421 1102 82-83 0.334846653913349 1102 84-85 0.291686331783108 1102 86-87 0.27705718671272095 1102 88-89 0.27380511407312014 1102 90-91 0.25459229702317643 1102 92-93 0.2548855707157749 1102 94-95 0.294108397568678 1102 96-97 0.3164806414502692 1102 98-99 0.24661517625302665 1102 100 0.17939606725230206 1103 1 0.35006946862782584 1103 2 0.37661840025916504 1103 3 0.40221398152164056 1103 4 0.32765228930232837 1103 5 0.3335867841210316 1103 6 0.331897520732241 1103 7 0.3617466989972584 1103 8 0.387234754838957 1103 9 0.42882350982274176 1103 10-11 0.39745687635970484 1103 12-13 0.43627754733181945 1103 14-15 0.5060123638852261 1103 16-17 0.47014037952259713 1103 18-19 0.4614724991647492 1103 20-21 0.4769255114821789 1103 22-23 0.5357193129700377 1103 24-25 0.554102498432961 1103 26-27 0.5053741105722693 1103 28-29 0.5036317113332061 1103 30-31 0.4708293874558649 1103 32-33 0.46302669333312707 1103 34-35 0.4668051619323563 1103 36-37 0.461257216782478 1103 38-39 0.4492550923203069 1103 40-41 0.45511124352307064 1103 42-43 0.44913185561547664 1103 44-45 0.4602934518475763 1103 46-47 0.5165475571056888 1103 48-49 0.5458390269380828 1103 50-51 0.47222509392553036 1103 52-53 0.4339620773077115 1103 54-55 0.543429817141849 1103 56-57 0.578426807369631 1103 58-59 0.4907743044228994 1103 60-61 0.5109328666436639 1103 62-63 0.49974048482332023 1103 64-65 0.4619254383054212 1103 66-67 0.43628939765309127 1103 68-69 0.44008591904378846 1103 70-71 0.39844061382836315 1103 72-73 0.3555299600077788 1103 74-75 0.37673183843303093 1103 76-77 0.3910296446798398 1103 78-79 0.38350452123113854 1103 80-81 0.3285174484586477 1103 82-83 0.31060294117181897 1103 84-85 0.30392922748580276 1103 86-87 0.25876366263223716 1103 88-89 0.252974052650238 1103 90-91 0.22230315652722865 1103 92-93 0.23530914254704527 1103 94-95 0.3316552278904865 1103 96-97 0.3014173001803364 1103 98-99 0.2599914613635548 1103 100 0.26543257503068673 1104 1 0.3705079721258393 1104 2 0.39948425328562465 1104 3 0.3777069745537389 1104 4 0.32609893701368264 1104 5 0.3187256569336867 1104 6 0.2805115315583606 1104 7 0.30411563035417544 1104 8 0.3150050349941367 1104 9 0.36413541638493996 1104 10-11 0.32851032474501807 1104 12-13 0.3639452713481788 1104 14-15 0.4358029033937356 1104 16-17 0.39244102542783565 1104 18-19 0.4116498758259226 1104 20-21 0.3494448509436978 1104 22-23 0.405035359245872 1104 24-25 0.3946825401370191 1104 26-27 0.34700944657245003 1104 28-29 0.37483454683436435 1104 30-31 0.32375349809903753 1104 32-33 0.2887900165050681 1104 34-35 0.3020424225699614 1104 36-37 0.30333438263892276 1104 38-39 0.3238023569665316 1104 40-41 0.32961449898682105 1104 42-43 0.2941865237059389 1104 44-45 0.3311784623558509 1104 46-47 0.36724466210822726 1104 48-49 0.4083866151037796 1104 50-51 0.3358562110919152 1104 52-53 0.35768299834494854 1104 54-55 0.5131223966007568 1104 56-57 0.5479003486276994 1104 58-59 0.5027617749523756 1104 60-61 0.4757477242684942 1104 62-63 0.4301181965304366 1104 64-65 0.4058432374540786 1104 66-67 0.4113420843154003 1104 68-69 0.427284628628648 1104 70-71 0.3979519430841165 1104 72-73 0.387593347868723 1104 74-75 0.36862327857573973 1104 76-77 0.38018139167404996 1104 78-79 0.37412235558497287 1104 80-81 0.3137372271432568 1104 82-83 0.3185979221876103 1104 84-85 0.29022345565410745 1104 86-87 0.2634237678972191 1104 88-89 0.30294250737852835 1104 90-91 0.2647805893042765 1104 92-93 0.2867295701721737 1104 94-95 0.32573360937498563 1104 96-97 0.2941421871063987 1104 98-99 0.2725268372935332 1104 100 0.19457920565313458 1105 1 0.3068235881055834 1105 2 0.33970123602466273 1105 3 0.32910840593314106 1105 4 0.2935537471052001 1105 5 0.31238509904436285 1105 6 0.2629876658798551 1105 7 0.2828033880873875 1105 8 0.31244707788083304 1105 9 0.3103039348612171 1105 10-11 0.3042317323698782 1105 12-13 0.32001016077617805 1105 14-15 0.3749056127337056 1105 16-17 0.3526560526534439 1105 18-19 0.3645564152922276 1105 20-21 0.36773936429536747 1105 22-23 0.3966946347382816 1105 24-25 0.42480379324337036 1105 26-27 0.4025629288691306 1105 28-29 0.40500241552501137 1105 30-31 0.41215216353538153 1105 32-33 0.3903519335803125 1105 34-35 0.4337996616706832 1105 36-37 0.41982331875529866 1105 38-39 0.46241573700825 1105 40-41 0.42579909637662894 1105 42-43 0.4555023015312827 1105 44-45 0.4658896905436478 1105 46-47 0.47800721925634804 1105 48-49 0.5073468055338424 1105 50-51 0.38089546578963507 1105 52-53 0.4485594636104935 1105 54-55 0.515743363802379 1105 56-57 0.555809302816904 1105 58-59 0.46642240294072224 1105 60-61 0.4350600634603765 1105 62-63 0.4653487516519519 1105 64-65 0.43373326174941695 1105 66-67 0.3711892896441569 1105 68-69 0.37427372288117766 1105 70-71 0.4074840321529436 1105 72-73 0.3647371181168566 1105 74-75 0.39927060043873297 1105 76-77 0.4083514929091976 1105 78-79 0.41701390122899085 1105 80-81 0.3627870840882821 1105 82-83 0.34406925489600226 1105 84-85 0.3743169537683855 1105 86-87 0.28001544745083606 1105 88-89 0.32924478613290376 1105 90-91 0.2909156685548524 1105 92-93 0.3155361813089108 1105 94-95 0.34475655823105456 1105 96-97 0.30582417107279625 1105 98-99 0.27736243830936047 1105 100 0.30999120930717794 1106 1 0.25677990427623243 1106 2 0.3090491142707741 1106 3 0.27871309303730385 1106 4 0.27516780875247804 1106 5 0.26705664239702287 1106 6 0.24382150266230695 1106 7 0.25173291737890224 1106 8 0.25957593223887443 1106 9 0.25603315660031 1106 10-11 0.23964030899963973 1106 12-13 0.24638648071596236 1106 14-15 0.3423054391676459 1106 16-17 0.3070195356821941 1106 18-19 0.2831583971852609 1106 20-21 0.3143669070415527 1106 22-23 0.29169870047155655 1106 24-25 0.3096271289386081 1106 26-27 0.34590739366078793 1106 28-29 0.3162695043958834 1106 30-31 0.2806332989343616 1106 32-33 0.312499740636234 1106 34-35 0.3239925451248453 1106 36-37 0.2738905817641708 1106 38-39 0.2808734222897442 1106 40-41 0.31686570777881684 1106 42-43 0.3235084988933963 1106 44-45 0.3406173240790267 1106 46-47 0.31123072620830783 1106 48-49 0.3462976526355277 1106 50-51 0.2567149468137586 1106 52-53 0.2983622235991277 1106 54-55 0.39489303036241097 1106 56-57 0.44425081171817027 1106 58-59 0.37303431265571163 1106 60-61 0.3715067988099392 1106 62-63 0.3661987279742007 1106 64-65 0.32738753218907846 1106 66-67 0.3124001421752709 1106 68-69 0.2712136602520374 1106 70-71 0.32380409837900714 1106 72-73 0.2987274979665244 1106 74-75 0.3092679348009568 1106 76-77 0.30667995242895785 1106 78-79 0.33081174174344596 1106 80-81 0.32104745700910087 1106 82-83 0.3495127092835624 1106 84-85 0.32334181916141347 1106 86-87 0.24628794479732719 1106 88-89 0.2740604833641349 1106 90-91 0.2673778555867763 1106 92-93 0.24201589798925838 1106 94-95 0.24395604385471614 1106 96-97 0.2558323580853532 1106 98-99 0.20638880826370354 1106 100 0.21239404153478603 1107 1 0.21395837044106614 1107 2 0.2600589290789479 1107 3 0.2649873607765656 1107 4 0.25465450530936096 1107 5 0.260856786440506 1107 6 0.22138397870944004 1107 7 0.22975837008097244 1107 8 0.2377115303870525 1107 9 0.25114113796750104 1107 10-11 0.25679475598671075 1107 12-13 0.245542187782668 1107 14-15 0.30677182839288974 1107 16-17 0.29876364450454673 1107 18-19 0.2903589193665326 1107 20-21 0.27423334208454264 1107 22-23 0.3114879726036861 1107 24-25 0.3066211507544594 1107 26-27 0.28626966819889077 1107 28-29 0.3056195490160789 1107 30-31 0.26542072527688276 1107 32-33 0.23645078335706415 1107 34-35 0.28365089112010367 1107 36-37 0.2603468745487163 1107 38-39 0.29107452529597566 1107 40-41 0.28807208861835676 1107 42-43 0.33295978836885354 1107 44-45 0.3515479796683252 1107 46-47 0.33320256291193573 1107 48-49 0.39955204231074504 1107 50-51 0.29759560517548067 1107 52-53 0.25726989820520885 1107 54-55 0.374607222630722 1107 56-57 0.384951109279946 1107 58-59 0.3704428868061598 1107 60-61 0.3904585807068557 1107 62-63 0.352894116622295 1107 64-65 0.3143694062293463 1107 66-67 0.32847542531818164 1107 68-69 0.3251118762422962 1107 70-71 0.31899901356909055 1107 72-73 0.33726400995410444 1107 74-75 0.3150446354135852 1107 76-77 0.30277120336713637 1107 78-79 0.32536349248007923 1107 80-81 0.2810578341410732 1107 82-83 0.31957940615494707 1107 84-85 0.32375318848385604 1107 86-87 0.27127813786715294 1107 88-89 0.25362192023971275 1107 90-91 0.29519866568730535 1107 92-93 0.30501754901641576 1107 94-95 0.28824337832179125 1107 96-97 0.30863674859709533 1107 98-99 0.29988910243407574 1107 100 0.28904058596468474 1108 1 0.11883937000805744 1108 2 0.14988937390512902 1108 3 0.18935166505863776 1108 4 0.14932409917348366 1108 5 0.1534829873403254 1108 6 0.1280715711174949 1108 7 0.1386546403210005 1108 8 0.18781619001523353 1108 9 0.19061122606252212 1108 10-11 0.1389939000755689 1108 12-13 0.14557763873127527 1108 14-15 0.22594579017518868 1108 16-17 0.17434552294739802 1108 18-19 0.15701991008444338 1108 20-21 0.14248899067321474 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-1.2237387976590277 2316 92-93 -1.032289422322087 2316 94-95 -1.3193656746841285 2316 96-97 -1.5068523262817735 2316 98-99 -1.3549562661323407 2316 100 -1.1415726590488724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14910.0 3 3687.0 4 2635.0 5 2186.0 6 3693.0 7 17250.0 8 34368.0 9 41571.0 10 43677.0 11 41153.0 12 40350.0 13 43002.0 14 48828.0 15 59190.0 16 73785.0 17 92042.0 18 100662.0 19 112353.0 20 128505.0 21 156045.0 22 198855.0 23 286163.0 24 405100.0 25 484082.0 26 479396.0 27 424471.0 28 400957.0 29 402466.0 30 453605.0 31 548860.0 32 684267.0 33 882512.0 34 1216000.0 35 1845717.0 36 3215274.0 37 6036818.0 38 7515494.0 39 2248697.0 40 4365.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.491015619142734 32.62993431318977 27.02718102562368 24.851869042043813 2 20.600526564581354 32.254479344748766 29.027512326474223 18.11748176419566 3 24.597457063263793 25.892171759121148 27.082173611660735 22.42819756595432 4 18.786059997266754 28.189764130842903 25.433370574513393 27.590805297376946 5 21.825145691144524 31.967818116826507 26.57714129136529 19.629894900663672 6 27.27890336732144 27.518471740958546 26.16283557059813 19.039789321121887 7 20.973630645405805 26.456526617933108 31.94316822259948 20.626674514061612 8 25.828427163789623 26.62616939252767 26.691107723199035 20.854295720483666 9 25.321669266030078 25.104894703610842 28.43936817471478 21.134067855644297 10-11 20.701310598106613 29.423592685595267 29.150654367036573 20.72444234926155 12-13 20.645843832797528 26.81936967411663 28.941422566567844 23.593363926518002 14-15 20.625990162366325 32.3130827603797 26.26915332658645 20.791773750667527 16-17 23.488269933947404 26.63573998141999 26.296811312728835 23.57917877190377 18-19 20.65276015385687 29.421672806162597 26.342724786895737 23.582842253084795 20-21 23.436625949025522 29.573280755400017 26.27843750888478 20.711655786689676 22-23 23.446714583351113 26.813884079758683 28.985598416711827 20.753802920178373 24-25 20.761479432186267 29.40738859520591 26.13463662143455 23.696495351173276 26-27 20.798767698581504 26.68947090556339 31.751692133215432 20.76006926263967 28-29 20.774943487754133 26.80076268847758 31.714748515899526 20.70954530786876 30-31 20.718798500569807 26.7098284255159 31.736278374935633 20.835094698978658 32-33 20.842337986131305 26.853811165407894 31.518687192546736 20.78516365591407 34-35 20.869480249289428 26.982589472091504 31.392177135452314 20.75575314316675 36-37 20.79773698214652 26.920875779273466 31.476059141719404 20.805328096860606 38-39 20.835282957896975 26.902357575427832 31.510484341845046 20.75187512483015 40-41 20.862174396092925 26.958598442119357 31.470676305488066 20.708550856299652 42-43 20.86806959422598 26.98758728418444 31.41163903578305 20.73270408580653 44-45 20.88910414597804 27.066189446519207 31.385070936949656 20.659635470553102 46-47 20.901696912500423 27.168904797534104 31.31492426158193 20.61447402838355 48-49 20.9857670992968 27.16847762249958 31.18266446383542 20.6630908143682 50-51 21.019221986372163 27.23598549865739 31.12133080387362 20.623461711096823 52-53 21.146139903155756 27.348634953261726 30.87711206096988 20.62811308261264 54-55 21.47777898866476 27.559945120338224 30.208097581953343 20.754178309043674 56-57 21.922738029141733 28.026445130833373 29.25316976819406 20.797647071830834 58-59 22.064056030095276 28.46736088895944 28.671762442008635 20.796820638936644 60-61 22.27073162434271 28.66374136133971 28.334082590735253 20.731444423582325 62-63 22.355608258637538 28.808696249589904 28.155289138122885 20.680406353649673 64-65 22.496061755977863 28.874591463142192 28.060200325838515 20.56914645504143 66-67 22.628330781247694 28.720977462390852 28.167225677424735 20.483466078936722 68-69 22.782982095160197 28.585067572084878 28.307544163524035 20.32440616923089 70-71 22.914225270109448 28.487062818784874 28.358373903615636 20.240338007490045 72-73 23.117803221060985 28.41696285047727 28.302294396551808 20.16293953190994 74-75 23.34749143267139 28.468676532987292 28.181604679281474 20.00222735505985 76-77 23.678144273690666 28.5480280639192 27.87685422074919 19.89697344164094 78-79 23.94419772472665 28.666141742933508 27.54692103475656 19.84273949758328 80-81 24.227973824504385 28.802053564150576 27.251811642418282 19.718160968926757 82-83 24.433511999516988 28.927176958343342 26.946969506134806 19.69234153600486 84-85 24.59779245788317 28.938619246864832 26.786538984845514 19.67704931040648 86-87 24.808308860536314 28.910674428525883 26.68385174453608 19.597164966401724 88-89 25.045979492967895 28.908745541708548 26.529113215931766 19.516161749391784 90-91 25.253610774323736 28.838149710605553 26.425135775868892 19.483103739201816 92-93 25.412853925287344 28.850373891627896 26.356849463887343 19.37992271919742 94-95 25.5854154280922 28.830671517618377 26.250417543413647 19.333495510875778 96-97 25.729619147692777 28.762004522653424 26.172032449693173 19.336343879960623 98-99 25.871148035662824 28.753796379730563 26.120707814272 19.254347770334622 100 25.961568419307422 28.763908268712285 26.005781539132595 19.2687417728477 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33279.0 1 24320.0 2 13268.0 3 10564.0 4 9804.0 5 9633.5 6 9753.0 7 9921.0 8 10011.5 9 10207.5 10 10966.0 11 13880.5 12 105994.5 13 131069.0 14 64565.0 15 66622.5 16 74756.5 17 83216.0 18 91235.0 19 99008.0 20 108882.0 21 124740.0 22 146856.5 23 173168.5 24 200081.0 25 229751.5 26 265723.5 27 308160.5 28 360195.5 29 414322.5 30 458752.5 31 500678.5 32 548628.0 33 590613.0 34 627877.5 35 673791.0 36 722268.0 37 766900.0 38 813301.0 39 848105.5 40 865030.0 41 890968.0 42 926801.0 43 940890.5 44 943541.0 45 959269.0 46 976552.5 47 987829.0 48 989208.5 49 983984.5 50 976400.5 51 963526.5 52 942022.0 53 898458.0 54 838293.0 55 778367.5 56 716476.5 57 646529.5 58 570123.5 59 488118.5 60 402082.0 61 323019.5 62 253108.5 63 190748.5 64 141434.0 65 104293.5 66 76804.5 67 57240.0 68 42938.5 69 33264.0 70 26368.0 71 21443.5 72 18003.5 73 14559.5 74 11702.5 75 9707.0 76 7799.5 77 5688.5 78 4099.5 79 3113.0 80 2484.5 81 2044.0 82 1630.5 83 1341.0 84 1139.5 85 949.5 86 801.5 87 711.5 88 629.5 89 561.0 90 473.0 91 401.5 92 363.5 93 317.5 94 263.5 95 221.5 96 190.0 97 149.5 98 114.5 99 87.0 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.402879297951366 2 0.3863058200518314 3 0.16902030080862387 4 0.17578930928016473 5 0.2737575960760728 6 0.1027854313572355 7 0.13718616450788318 8 0.14273265323494874 9 0.13599142930305505 10-11 0.13247147543650467 12-13 0.13387806775614247 14-15 0.14047342285488854 16-17 0.14469146328007396 18-19 0.15619773576145665 20-21 0.1371288588948609 22-23 0.09915433933209648 24-25 0.09441360225479875 26-27 0.0891831626662197 28-29 0.09873583470366104 30-31 0.10349046405078235 32-33 0.10448028827571265 34-35 0.10516100949706823 36-37 0.10106452643283916 38-39 0.10039248788012332 40-41 0.10130937768847981 42-43 0.0635744997801722 44-45 0.06643630736383033 46-47 0.08531937512153566 48-49 0.08950094833843417 50-51 0.09891990727882352 52-53 0.10419723327805715 54-55 0.10692706429839122 56-57 0.10722053849841442 58-59 0.1067412551895008 60-61 0.10442645573014626 62-63 0.10920366001573091 64-65 0.11581290738430058 66-67 0.1243097669151496 68-69 0.12926236110725697 70-71 0.12789049946217815 72-73 0.11502625760554017 74-75 0.11260205652132493 76-77 0.11437158439010382 78-79 0.11330014307995998 80-81 0.10731604785345154 82-83 0.1104869584406844 84-85 0.11664297050625967 86-87 0.1299743399357156 88-89 0.13782347238604006 90-91 0.14499362014873687 92-93 0.14405589193564503 94-95 0.13369920478216382 96-97 0.13033206588367285 98-99 0.12556354426672797 100 0.12002573820830216 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8792991E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.52562239080852 #Duplication Level Percentage of deduplicated Percentage of total 1 68.16255780517325 27.623300788041092 2 15.750404188850018 12.765898653198892 3 6.0345643603987105 7.336634296876474 4 2.8965536602828097 4.695385594853416 5 1.6148172529471667 3.2720737111549805 6 1.0893908862577781 2.648894621548257 7 0.7573143429755365 2.1483444566198893 8 0.5570958102901268 1.8061323554655344 9 0.4437676212018998 1.618556314148599 >10 2.3949153051495737 17.46328564835576 >50 0.16691534975303238 4.809114505988934 >100 0.13045279849450675 8.227199541831947 >500 6.748111837083796E-4 0.1710981543768864 >1k 5.329914021977758E-4 0.42683060946964974 >5k 1.7126255928631713E-5 0.04827826967390191 >10k+ 2.568938385654424E-5 4.938972478395939 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1358970 4.719794480538684 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 35041 0.1216997567220439 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29217 0.10147261185890692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014378499267408515 2.4311472191270437E-5 0.0 0.0 3.8203742014853546E-5 2 0.0014691075338439136 3.125760710306199E-5 0.0 0.0 4.167680947074932E-5 3 0.001479526736211601 4.51498769266451E-5 0.0 0.0 4.51498769266451E-5 4 0.0014968920734910799 5.556907929433243E-5 0.0 0.0 4.51498769266451E-5 5 0.001510784343314663 6.946134911791554E-5 0.0 0.0 4.51498769266451E-5 6 0.0015212035456823503 6.946134911791554E-5 0.0 3.4730674558957768E-6 4.51498769266451E-5 7 0.0015212035456823503 6.946134911791554E-5 0.0 3.4730674558957768E-6 4.51498769266451E-5 8 0.0015281496805941417 7.293441657381131E-5 0.0 3.4730674558957768E-6 4.51498769266451E-5 9 0.0015455150178736207 7.293441657381131E-5 0.0 3.4730674558957768E-6 4.51498769266451E-5 10-11 0.001571563023792839 8.335361894149865E-5 0.0 3.4730674558957768E-6 4.51498769266451E-5 12-13 0.0016149763669915363 9.724588876508175E-5 0.0 3.4730674558957768E-6 4.6886410654592984E-5 14-15 0.0016635993113740771 1.0766509113276909E-4 0.0 3.4730674558957768E-6 5.556907929433243E-5 16-17 0.0017139587894845658 1.198208272284043E-4 0.0 6.9461349117915535E-6 5.904214675022821E-5 18-19 0.001790366273514273 1.3197656332403953E-4 0.0 1.7365337279478886E-5 6.077868047817609E-5 20-21 0.001854618021448345 1.6323417042710152E-4 0.0 2.083840473537466E-5 6.251521420612398E-5 22-23 0.001977911916132645 1.805995077065804E-4 0.0 3.125760710306199E-5 6.772481538996765E-5 24-25 0.0023356378640899097 1.9275524380221563E-4 0.0 3.473067455895777E-5 8.856322012534231E-5 26-27 0.003796062729294084 2.0838404735374662E-4 0.0 3.8203742014853546E-5 9.550935503713386E-5 28-29 0.008260690943848105 2.1880324972143393E-4 0.0 4.167680947074932E-5 1.059285574048212E-4 30-31 0.019270314779037716 2.240128509052776E-4 0.0 4.167680947074932E-5 1.1808429350045642E-4 32-33 0.042256811735883915 2.3790512072886073E-4 0.0 4.167680947074932E-5 1.3024002959609164E-4 34-35 0.08405344203386164 2.5006085682449593E-4 0.0 4.167680947074932E-5 1.371861645078832E-4 36-37 0.1519744857350874 2.570069917362875E-4 1.7365337279478884E-6 4.167680947074932E-5 1.5281496805941418E-4 38-39 0.25088397381154326 2.847915313834537E-4 3.4730674558957768E-6 4.167680947074932E-5 1.5281496805941418E-4 40-41 0.38431748893333106 2.969472674790889E-4 3.4730674558957768E-6 4.167680947074932E-5 1.5455150178736207E-4 42-43 0.5534385086981759 3.073664698467763E-4 3.4730674558957768E-6 4.167680947074932E-5 1.667072378829973E-4 44-45 0.765705445467614 3.160491384865157E-4 3.4730674558957768E-6 4.341334319869721E-5 1.7365337279478884E-4 46-47 1.0282815008694304 3.212587396703594E-4 3.4730674558957768E-6 4.51498769266451E-5 1.788629739786325E-4 48-49 1.3439624942056212 3.3688754322189034E-4 5.209601183843665E-6 4.6886410654592984E-5 1.8407257516247618E-4 50-51 1.7083810431503972 3.4904327931752554E-4 6.9461349117915535E-6 5.2096011838436655E-5 1.8407257516247618E-4 52-53 2.1109842322390193 3.664086165970045E-4 6.9461349117915535E-6 6.598828166201976E-5 1.9101871007426772E-4 54-55 2.5566951345902202 3.8377395387648335E-4 6.9461349117915535E-6 6.772481538996765E-5 1.944917775301635E-4 56-57 3.0583658363245414 4.0461235861185803E-4 6.9461349117915535E-6 7.119788284586342E-5 2.0317444616990295E-4 58-59 3.616227296427801 4.219776958913369E-4 6.9461349117915535E-6 7.293441657381131E-5 2.1012058108169448E-4 60-61 4.223699788604803 4.445526343546594E-4 6.9461349117915535E-6 7.293441657381131E-5 2.1880324972143393E-4 62-63 4.865295168535981 4.5844490417824255E-4 6.9461349117915535E-6 7.640748402970709E-5 2.257493846332255E-4 64-65 5.539191812340718 4.706006402738778E-4 6.9461349117915535E-6 8.335361894149864E-5 2.3443205327296495E-4 66-67 6.253688614704878 4.7928330891361723E-4 6.9461349117915535E-6 9.203628758123809E-5 2.413781881847565E-4 68-69 7.009827495865226 4.8796597755335666E-4 6.9461349117915535E-6 9.377282130918598E-5 2.4658778936860015E-4 70-71 7.800320223765568 4.949121124651482E-4 6.9461349117915535E-6 9.724588876508175E-5 2.4832432309654807E-4 72-73 8.618941324991212 5.00121713648992E-4 6.9461349117915535E-6 9.724588876508175E-5 2.535339242803917E-4 74-75 9.455075368863207 5.122774497446271E-4 6.9461349117915535E-6 1.0245548994892542E-4 2.5527045800833963E-4 76-77 10.317158436231928 5.400619893917933E-4 6.9461349117915535E-6 1.0766509113276908E-4 2.622165929201312E-4 78-79 11.202653451320844 5.487446580315328E-4 6.9461349117915535E-6 1.1113815858866486E-4 2.6395312664807905E-4 80-81 12.109542561938078 5.678465290389594E-4 6.9461349117915535E-6 1.1461122604456064E-4 2.6395312664807905E-4 82-83 13.033048563798044 5.817387988625426E-4 6.9461349117915535E-6 1.1808429350045642E-4 2.656896603760269E-4 84-85 13.969234734939485 6.008406698699694E-4 6.9461349117915535E-6 1.2676696214019586E-4 2.6742619410397483E-4 86-87 14.920450605496317 6.164694734215004E-4 6.9461349117915535E-6 1.2850349586814375E-4 2.708992615598706E-4 88 15.63787173065834 6.286252095171356E-4 6.9461349117915535E-6 1.3197656332403953E-4 2.708992615598706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 174920 0.0 84.56137 8 AGTGGTA 177490 0.0 83.665825 5 TGGTATC 177425 0.0 83.20015 7 GTGGTAT 178310 0.0 83.18959 6 GTATCAA 178150 0.0 82.93902 9 AAGCAGT 184215 0.0 81.05419 1 GCAGTGG 186300 0.0 80.17984 3 CAGTGGT 189195 0.0 78.3638 4 AGCAGTG 193260 0.0 77.362854 2 CAACGCA 172235 0.0 44.446068 12-13 ATCAACG 173660 0.0 44.1824 10-11 ACGCAGA 174900 0.0 43.794014 14-15 AGAGTAC 174600 0.0 43.18394 18-19 TCAACGC 172055 0.0 43.04723 12-13 AACGCAG 173760 0.0 42.71225 14-15 GAGTACT 169985 0.0 42.616978 20-21 CGCAGAG 176505 0.0 41.9775 16-17 AGTACTT 179595 0.0 41.64649 20-21 TATCAAC 178145 0.0 41.4395 10-11 GTACTTT 181325 0.0 39.935265 22-23 >>END_MODULE