FastQCFastQC Report
Thu 26 May 2016
SRR1660741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28792991
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13776804.784775572638494No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT363800.12635019404548836No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT296850.10309800742826614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1850850.084.67818
AGTGGTA1860750.083.60995
TGGTATC1878950.083.384037
GTGGTAT1868850.083.234966
GTATCAA1887650.082.922429
AAGCAGT1834900.082.501871
GCAGTGG1897900.081.899363
AGCAGTG1968000.078.750292
CAGTGGT1978350.078.5122454
CAACGCA1823300.044.2310212-13
ATCAACG1833750.044.06599810-11
ACGCAGA1851600.043.6779414-15
AGAGTAC1873550.043.07906318-19
TCAACGC1816000.043.00658812-13
AACGCAG1836700.042.62539714-15
GAGTACT1818850.042.60754820-21
CGCAGAG1866750.041.94426716-17
AGTACTT1913700.041.84120620-21
TATCAAC1885850.041.42611310-11
GTACTTT1927350.040.15235522-23
Produced by FastQC (version 0.11.5)