##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660741_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28792991 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.181495975878295 34.0 31.0 34.0 27.0 34.0 2 31.84233687288688 34.0 31.0 34.0 28.0 34.0 3 31.62419986169551 34.0 31.0 34.0 30.0 34.0 4 35.55185517197571 37.0 35.0 37.0 33.0 37.0 5 35.88632365425322 37.0 35.0 37.0 33.0 37.0 6 35.343570732196596 37.0 35.0 37.0 33.0 37.0 7 35.560095476013586 37.0 35.0 37.0 33.0 37.0 8 35.92499896936723 37.0 35.0 37.0 33.0 37.0 9 37.73131679859171 39.0 37.0 39.0 35.0 39.0 10-11 37.759151246218224 39.0 37.5 39.0 35.0 39.0 12-13 37.82113433439409 39.0 38.0 39.0 35.0 39.0 14-15 39.26319915843408 41.0 39.0 41.0 36.0 41.0 16-17 39.230441081998045 41.0 39.0 41.0 36.0 41.0 18-19 39.22749470522184 41.0 39.0 41.0 36.0 41.0 20-21 39.16919457933356 40.5 39.0 41.0 36.0 41.0 22-23 39.05601521217438 40.0 38.5 41.0 35.5 41.0 24-25 39.05823807953817 40.0 39.0 41.0 35.5 41.0 26-27 39.00199619761629 40.0 39.0 41.0 35.0 41.0 28-29 38.91844581203807 40.0 38.5 41.0 35.0 41.0 30-31 38.821512707728075 40.0 38.0 41.0 35.0 41.0 32-33 38.6969219001944 40.0 38.0 41.0 35.0 41.0 34-35 38.60685643947167 40.0 38.0 41.0 34.0 41.0 36-37 38.48107410932056 40.0 38.0 41.0 34.0 41.0 38-39 38.108168373337804 40.0 37.0 41.0 34.0 41.0 40-41 37.97503017314179 40.0 37.0 41.0 33.0 41.0 42-43 37.8080253281085 40.0 37.0 41.0 33.0 41.0 44-45 37.6816599914889 40.0 36.0 41.0 33.0 41.0 46-47 37.53048266156163 40.0 36.0 41.0 33.0 41.0 48-49 37.57401230042409 40.0 36.0 41.0 33.0 41.0 50-51 37.57625324510399 40.0 36.0 41.0 33.0 41.0 52-53 37.338159727830984 40.0 36.0 41.0 32.5 41.0 54-55 36.85648351017093 40.0 35.5 41.0 30.5 41.0 56-57 36.412518223619074 39.0 35.0 41.0 29.5 41.0 58-59 36.283720888878825 39.0 35.0 41.0 29.0 41.0 60-61 36.134676977463016 39.0 35.0 41.0 29.0 41.0 62-63 35.894290766805014 39.0 35.0 41.0 28.5 41.0 64-65 35.62482273897838 38.0 35.0 40.5 28.0 41.0 66-67 35.336638767400025 37.0 35.0 40.0 28.0 41.0 68-69 35.06109504219273 37.0 35.0 40.0 28.0 41.0 70-71 34.752898891261424 36.0 34.0 39.0 28.0 41.0 72-73 34.40835042111463 36.0 34.0 39.0 27.0 41.0 74-75 34.03114023478839 35.0 34.0 38.5 26.5 40.0 76-77 32.32340497380074 34.5 31.5 36.5 24.5 39.0 78-79 33.264968616841514 35.0 33.5 37.0 26.0 39.0 80-81 33.17470185712905 35.0 34.0 36.5 26.0 39.0 82-83 32.92470193527306 35.0 34.0 36.0 26.0 38.0 84-85 32.682812858865546 35.0 34.0 36.0 25.5 37.0 86-87 32.47457002643456 35.0 33.5 35.0 25.0 37.0 88-89 32.30626764339974 35.0 33.0 35.0 25.0 36.0 90-91 32.1494757178926 35.0 33.0 35.0 25.0 36.0 92-93 32.01075294678486 35.0 33.0 35.0 24.0 36.0 94-95 31.91032114378114 35.0 33.0 35.0 24.0 36.0 96-97 31.81412887949015 35.0 33.0 35.0 24.0 35.0 98-99 31.72334574410835 35.0 33.0 35.0 23.5 35.0 100 31.669947384069964 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6092744076296022 1101 2 1.2026711392080962 1101 3 1.554503158092377 1101 4 0.964714108252771 1101 5 0.5902678748350993 1101 6 1.1480861227198105 1101 7 0.8396199428856193 1101 8 0.49118386660178004 1101 9 0.5294498933612104 1101 10-11 0.4241899264403841 1101 12-13 0.3994004628240049 1101 14-15 0.5370097588958416 1101 16-17 0.5652408974056513 1101 18-19 0.5142476788842103 1101 20-21 0.5106681413100347 1101 22-23 0.5014997637183285 1101 24-25 0.5091843260675972 1101 26-27 0.5187687353984671 1101 28-29 0.4913493128437665 1101 30-31 0.507548062125089 1101 32-33 0.5351806351964541 1101 34-35 0.5525193535628645 1101 36-37 0.5178640669633694 1101 38-39 0.5401669505899278 1101 40-41 0.5374335850635106 1101 42-43 0.5330478832806875 1101 44-45 0.5121340615780028 1101 46-47 0.5078816148073955 1101 48-49 0.482725543664678 1101 50-51 0.46467578998876036 1101 52-53 0.46681482074707503 1101 54-55 0.49415548326699366 1101 56-57 0.542942725353555 1101 58-59 0.5134507089710922 1101 60-61 0.5123618173683226 1101 62-63 0.4821673340654513 1101 64-65 0.4883092144197434 1101 66-67 0.486839519948397 1101 68-69 0.44165903470953083 1101 70-71 0.46201077204652563 1101 72-73 0.42135937353059916 1101 74-75 0.44734937312439627 1101 76-77 0.40017573565872055 1101 78-79 0.47225873717837175 1101 80-81 0.46596447856900625 1101 82-83 0.4725706582072675 1101 84-85 0.46013566506930204 1101 86-87 0.4161953474263811 1101 88-89 0.404495425511044 1101 90-91 0.40205320480420426 1101 92-93 0.41850241836745994 1101 94-95 0.4122109691534561 1101 96-97 0.35470268936323635 1101 98-99 0.3691511871915658 1101 100 0.3790802265162334 1102 1 1.5199354976851431 1102 2 1.1492908976108431 1102 3 1.5038373689594486 1102 4 0.9264184307518804 1102 5 0.5016428027885667 1102 6 1.0630127600209036 1102 7 0.7922481857114718 1102 8 0.46101559012515025 1102 9 0.47895398831904146 1102 10-11 0.38947788100558256 1102 12-13 0.3615138386127228 1102 14-15 0.4767586836375415 1102 16-17 0.49116664958741296 1102 18-19 0.4481604486503059 1102 20-21 0.4347255677354056 1102 22-23 0.44807410835273487 1102 24-25 0.43659887798308716 1102 26-27 0.4614139573177525 1102 28-29 0.4506681981443563 1102 30-31 0.43596774216995016 1102 32-33 0.4578723681339554 1102 34-35 0.4312147400962445 1102 36-37 0.45081739185673086 1102 38-39 0.4731749915883441 1102 40-41 0.48452397982194384 1102 42-43 0.49014281143111305 1102 44-45 0.477630533543703 1102 46-47 0.4920279610584757 1102 48-49 0.483519489453748 1102 50-51 0.41640129360249745 1102 52-53 0.45196663115613944 1102 54-55 0.46413738496539025 1102 56-57 0.5083641065682372 1102 58-59 0.4711511739947767 1102 60-61 0.47041575787940815 1102 62-63 0.44002067880113316 1102 64-65 0.4172089762187028 1102 66-67 0.41987658916136894 1102 68-69 0.3710929955047746 1102 70-71 0.36923488490103296 1102 72-73 0.3320245524887966 1102 74-75 0.37171559653937436 1102 76-77 0.21279150749781905 1102 78-79 0.40692564166013767 1102 80-81 0.42923244535366933 1102 82-83 0.4211627981186723 1102 84-85 0.41575561391044147 1102 86-87 0.40622243399440805 1102 88-89 0.41324564060263924 1102 90-91 0.40873820465981225 1102 92-93 0.3584917499579703 1102 94-95 0.3339762811789839 1102 96-97 0.3407547204333099 1102 98-99 0.351575117299749 1102 100 0.3373054151946384 1103 1 1.4878096183234426 1103 2 1.0997609781746895 1103 3 1.4387204217674743 1103 4 0.8843238070538746 1103 5 0.49117207827450926 1103 6 1.0336648682678842 1103 7 0.772473333053675 1103 8 0.4365384531512291 1103 9 0.45265397370822313 1103 10-11 0.3431776512301994 1103 12-13 0.3130691157337253 1103 14-15 0.37348372683946707 1103 16-17 0.4003388582338303 1103 18-19 0.37706330629202256 1103 20-21 0.380948126033509 1103 22-23 0.3655670234341315 1103 24-25 0.38515934828776466 1103 26-27 0.4204120524475954 1103 28-29 0.3947960537170019 1103 30-31 0.38783098940339755 1103 32-33 0.40472355658172177 1103 34-35 0.39318691122932137 1103 36-37 0.4007468868722768 1103 38-39 0.3959669794030489 1103 40-41 0.4320899823256923 1103 42-43 0.4288346444909408 1103 44-45 0.4033160572806622 1103 46-47 0.4193866910861743 1103 48-49 0.37224724835977696 1103 50-51 0.37163899967158187 1103 52-53 0.38588254160062263 1103 54-55 0.41119164385170137 1103 56-57 0.4268458193856475 1103 58-59 0.4052847950209397 1103 60-61 0.4088704507190215 1103 62-63 0.36912610987285177 1103 64-65 0.35033170037873873 1103 66-67 0.3826257490394269 1103 68-69 0.34946898395367754 1103 70-71 0.36402148517394295 1103 72-73 0.34312005224925457 1103 74-75 0.3674547885559747 1103 76-77 0.24798119065609825 1103 78-79 0.3970873227085079 1103 80-81 0.39023586270419486 1103 82-83 0.3983382076236168 1103 84-85 0.3791593957874042 1103 86-87 0.34665999954898297 1103 88-89 0.34382171070912193 1103 90-91 0.3454437554025489 1103 92-93 0.33615559538596784 1103 94-95 0.33720304589812855 1103 96-97 0.38071128975801827 1103 98-99 0.3800910344924411 1103 100 0.3905176972081499 1104 1 1.4408689141546454 1104 2 1.0544937888513708 1104 3 1.3983269082622591 1104 4 0.8612584675973736 1104 5 0.4666672543204058 1104 6 1.0055941310099854 1104 7 0.7561685258213657 1104 8 0.40043244375331 1104 9 0.4042456731408137 1104 10-11 0.3008176576605379 1104 12-13 0.29443287721842637 1104 14-15 0.3424747953209746 1104 16-17 0.37322771446114444 1104 18-19 0.3293308225863143 1104 20-21 0.3171017751204275 1104 22-23 0.3220163511496139 1104 24-25 0.3151207530517368 1104 26-27 0.3124307371559567 1104 28-29 0.2745173203899043 1104 30-31 0.3074288959171483 1104 32-33 0.34752822384903936 1104 34-35 0.3587904118437848 1104 36-37 0.3566076568760863 1104 38-39 0.4004023751100405 1104 40-41 0.3546347682009312 1104 42-43 0.3850668035091118 1104 44-45 0.36753648935098937 1104 46-47 0.39148057199462727 1104 48-49 0.3338377671589896 1104 50-51 0.31215222121598885 1104 52-53 0.3296315161999672 1104 54-55 0.31884758722183193 1104 56-57 0.3898470288605296 1104 58-59 0.39578582441162524 1104 60-61 0.39127561019396495 1104 62-63 0.3541911972526606 1104 64-65 0.3141640508196275 1104 66-67 0.31972516682068886 1104 68-69 0.27411639858363657 1104 70-71 0.29817568433121977 1104 72-73 0.2741732860895425 1104 74-75 0.2815297626318909 1104 76-77 0.16716685285728516 1104 78-79 0.29874202607190625 1104 80-81 0.3046957349079591 1104 82-83 0.2997208281514858 1104 84-85 0.26088025541997695 1104 86-87 0.27704817700853823 1104 88-89 0.2688265190823387 1104 90-91 0.25425986441584314 1104 92-93 0.28997894243359923 1104 94-95 0.285116551291253 1104 96-97 0.2867321160650391 1104 98-99 0.2621838646503285 1104 100 0.32379564315207787 1105 1 1.3864594909237091 1105 2 1.0151032137308071 1105 3 1.341842502202649 1105 4 0.8217772940417021 1105 5 0.423506664240044 1105 6 0.9925206434321794 1105 7 0.7272767720833997 1105 8 0.3666056729323799 1105 9 0.34691213222207296 1105 10-11 0.2602851782653417 1105 12-13 0.2422434059233609 1105 14-15 0.3103485360921354 1105 16-17 0.30045672283146985 1105 18-19 0.2842535829447357 1105 20-21 0.28393135961115235 1105 22-23 0.2797069406847257 1105 24-25 0.30048401380530976 1105 26-27 0.2848911634418201 1105 28-29 0.28313042615479844 1105 30-31 0.2891712988895634 1105 32-33 0.3254768767565963 1105 34-35 0.3308724096921125 1105 36-37 0.31913473513075274 1105 38-39 0.3454949858648675 1105 40-41 0.3403486631519854 1105 42-43 0.324442613720187 1105 44-45 0.3256539435444097 1105 46-47 0.3284682289771865 1105 48-49 0.30923415235783835 1105 50-51 0.29369383235532354 1105 52-53 0.3007798859955244 1105 54-55 0.3427558829306534 1105 56-57 0.3614662259134036 1105 58-59 0.2744366631205253 1105 60-61 0.2692179782941295 1105 62-63 0.20623489679434215 1105 64-65 0.23338101458272575 1105 66-67 0.2368635017567584 1105 68-69 0.2212069529845948 1105 70-71 0.2618694172556246 1105 72-73 0.23408320110497272 1105 74-75 0.2636195021343184 1105 76-77 0.14693585060986436 1105 78-79 0.24927539931388054 1105 80-81 0.2677050199041915 1105 82-83 0.2843346016586068 1105 84-85 0.27160324595897123 1105 86-87 0.26495655253643946 1105 88-89 0.2533126137907118 1105 90-91 0.2714308148695608 1105 92-93 0.2646318916226704 1105 94-95 0.23561458312382655 1105 96-97 0.24987216623372888 1105 98-99 0.2328846157469222 1105 100 0.24309215597032363 1106 1 1.3707937053270776 1106 2 0.9976806892064012 1106 3 1.3297342901303502 1106 4 0.7848022432229698 1106 5 0.39678501615494355 1106 6 0.937706422291761 1106 7 0.6713085128485972 1106 8 0.33064817301032434 1106 9 0.3250314849850042 1106 10-11 0.225256391767914 1106 12-13 0.19551466164533338 1106 14-15 0.26521897410584216 1106 16-17 0.2465991821509519 1106 18-19 0.20908496482184802 1106 20-21 0.2423225719179598 1106 22-23 0.2564637980282285 1106 24-25 0.2498086158639694 1106 26-27 0.23791421663995038 1106 28-29 0.23870723249754633 1106 30-31 0.21989638184857796 1106 32-33 0.27407940605681347 1106 34-35 0.29792335709429807 1106 36-37 0.27101187986220765 1106 38-39 0.2848011238846908 1106 40-41 0.25107614600295847 1106 42-43 0.2708308539857711 1106 44-45 0.2875319107499337 1106 46-47 0.28317826393890044 1106 48-49 0.2740715354866765 1106 50-51 0.268170115125578 1106 52-53 0.2701428916519788 1106 54-55 0.29651730428556533 1106 56-57 0.30583026497292565 1106 58-59 0.31653909314493234 1106 60-61 0.30443363082478214 1106 62-63 0.2799995686053407 1106 64-65 0.23758236825799628 1106 66-67 0.19850940077871115 1106 68-69 0.23415506827520716 1106 70-71 0.2140898033525076 1106 72-73 0.14015834297287455 1106 74-75 0.21677223624174502 1106 76-77 0.10186777602687158 1106 78-79 0.22764019505462585 1106 80-81 0.2577195112620032 1106 82-83 0.26442552197918445 1106 84-85 0.2742969908993942 1106 86-87 0.28389807399578615 1106 88-89 0.3033401746933464 1106 90-91 0.2942044605885741 1106 92-93 0.2714741143687789 1106 94-95 0.2677274812248811 1106 96-97 0.2696153675210695 1106 98-99 0.26992830012792624 1106 100 0.26531972734933973 1107 1 1.2977789986241923 1107 2 0.9156542608714879 1107 3 1.2699309890582775 1107 4 0.7922355887738775 1107 5 0.39164892488830105 1107 6 0.9441610347937939 1107 7 0.6618303700218036 1107 8 0.30121961530544183 1107 9 0.2791377028073754 1107 10-11 0.19062454150425623 1107 12-13 0.14840154394143212 1107 14-15 0.18928247968195677 1107 16-17 0.19125961806803105 1107 18-19 0.16788542142366225 1107 20-21 0.1752343115671593 1107 22-23 0.1996314655809428 1107 24-25 0.18611962067498666 1107 26-27 0.17553264056869722 1107 28-29 0.19106030822462827 1107 30-31 0.19013612425142412 1107 32-33 0.23381415864159294 1107 34-35 0.21012624090021603 1107 36-37 0.21035871871315948 1107 38-39 0.21099133553477856 1107 40-41 0.21707517434963108 1107 42-43 0.2585870435420574 1107 44-45 0.21565533731696718 1107 46-47 0.2695676973832022 1107 48-49 0.25044257844822937 1107 50-51 0.2028438620027586 1107 52-53 0.23182728237647154 1107 54-55 0.26800502038810947 1107 56-57 0.247469106704024 1107 58-59 0.17060291703418784 1107 60-61 0.1858149080491387 1107 62-63 0.14860175913869966 1107 64-65 0.13700328201547052 1107 66-67 0.17517120531834962 1107 68-69 0.18038175051285776 1107 70-71 0.156849811820436 1107 72-73 0.15595194234777665 1107 74-75 0.1902117071305014 1107 76-77 0.023870395311668346 1107 78-79 0.1388828952126957 1107 80-81 0.1851368321845186 1107 82-83 0.19621810788016347 1107 84-85 0.2252336422994432 1107 86-87 0.19876169682399336 1107 88-89 0.2062784062495382 1107 90-91 0.23956786083945758 1107 92-93 0.2225604221480424 1107 94-95 0.1872166629261045 1107 96-97 0.2048423568219988 1107 98-99 0.2440771347359707 1107 100 0.23797911719863762 1108 1 1.2504452585281918 1108 2 0.8964525309900147 1108 3 1.2332765549709457 1108 4 0.7520841337951722 1108 5 0.33828567802792975 1108 6 0.8910071702244053 1108 7 0.6057371444030721 1108 8 0.27199339530478284 1108 9 0.21687159791672883 1108 10-11 0.13276251365108038 1108 12-13 0.13586693071645328 1108 14-15 0.15005521978859804 1108 16-17 0.13297640818743872 1108 18-19 0.09893818584593106 1108 20-21 0.11869205579915132 1108 22-23 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-1.0185750696207734 2316 86-87 -0.7909053749820814 2316 88-89 -0.6960699665324768 2316 90-91 -0.7693473558440829 2316 92-93 -0.8596148620440935 2316 94-95 -0.8469534462499198 2316 96-97 -0.9415838762211806 2316 98-99 -1.1232903673140306 2316 100 -1.0257081943158433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 43.0 7 810.0 8 982.0 9 426.0 10 364.0 11 733.0 12 946.0 13 1499.0 14 3414.0 15 8280.0 16 17629.0 17 28315.0 18 36024.0 19 45937.0 20 61335.0 21 85610.0 22 126785.0 23 208702.0 24 350845.0 25 460361.0 26 465761.0 27 399936.0 28 360927.0 29 383659.0 30 441403.0 31 536175.0 32 660496.0 33 843063.0 34 1151538.0 35 1766038.0 36 3189550.0 37 6427226.0 38 8349552.0 39 2373893.0 40 4734.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.438022272596527 32.27728143932286 27.23595087477584 24.048745413304776 2 20.91067637346061 32.56060048544158 28.579280698699105 17.949442442398706 3 24.798394340634143 25.93331974413895 27.210775163933 22.057510751293908 4 19.1416318045908 28.20112452617845 25.422286006799837 27.234957662430915 5 21.732128275837205 32.19685504624512 26.500171934252176 19.570844743665496 6 28.125293282526755 27.42411749876643 25.775917457211055 18.674671761495752 7 20.912828314112872 26.4074767879513 32.13428656048977 20.54540833744605 8 26.01339947691993 26.646145414592443 26.583143804647776 20.757311303839852 9 25.553705066625415 25.1073533833286 28.281855122310844 21.05708642773514 10-11 20.6324634103394 29.565873197520087 29.189621633336195 20.61204175880432 12-13 20.55901280137978 26.815631165780278 29.003637690979645 23.621718341860294 14-15 20.515550121208317 32.55115281354411 26.21827131470989 20.71502575053769 16-17 23.55628780196728 26.588334260237538 26.23910676279797 23.616271174997213 18-19 20.572815793954856 29.520311036807534 26.282700189084213 23.6241729801534 20-21 23.47195878929631 29.649066707676763 26.262701565260443 20.61627293776648 22-23 23.458369940924147 26.769091744963813 29.09338911452065 20.679149199591386 24-25 20.668772826745844 29.494897195193463 26.09032179057423 23.74600818748646 26-27 20.725155510626415 26.615954322178382 31.97147777507043 20.68741239212478 28-29 20.692796274505007 26.74905273686684 31.940709246072796 20.61744174255536 30-31 20.648782192924525 26.639461613147454 31.951404001939192 20.76035219198883 32-33 20.811082967419903 26.775411555241636 31.728755627906025 20.68474984943244 34-35 20.803544807380074 26.900720065266725 31.61638897001226 20.679346157340937 36-37 20.731887375171578 26.833630179997442 31.704854415466777 20.729628029364203 38-39 20.759225720512163 26.839896019181243 31.74634155456631 20.654536705740284 40-41 20.81615605247169 26.86534107649301 31.686089257169165 20.632413613866134 42-43 20.791838368198867 26.894884176813193 31.63159114474024 20.6816863102477 44-45 20.82558689598808 26.990629995548783 31.582390083856083 20.60139302460706 46-47 20.877729206666494 27.099878138330574 31.46276110832556 20.55963154667737 48-49 20.903888856267592 27.121927754211306 31.355226122918666 20.618957266602436 50-51 20.93117781462371 27.21706350066004 31.2487319288515 20.603026755864754 52-53 21.054683062971037 27.332005875990916 30.98717616989281 20.626134891145238 54-55 21.323270543123815 27.545197115879777 30.324558805910456 20.806973535085955 56-57 21.730904296498984 28.061441463260937 29.27377830635789 20.933875933882188 58-59 21.816825133775772 28.57371994495781 28.612267125115388 20.997187796151035 60-61 21.89402128514216 28.818036334816004 28.248102616345257 21.039839763696573 62-63 21.866046800853198 28.994719618700888 28.051488928568947 21.087744651876967 64-65 21.881243365413077 29.08428480547168 27.917457477750823 21.11701435136442 66-67 21.850959648655394 28.97753156560118 28.01205664412807 21.159452141615358 68-69 21.76731621191075 28.888668198016536 28.17303036190776 21.17098522816495 70-71 21.72111829530622 28.798432696370508 28.213001371541846 21.267447636781426 72-73 21.66127894716849 28.744034673157625 28.175617609625032 21.419068770048856 74-75 21.73506822119469 28.77828313283158 28.039958078674974 21.446690567298752 76-77 21.75977054847725 28.861932589319174 27.85603357743759 21.522263284765994 78-79 21.820803205029247 29.005754454318073 27.51833239234191 21.655109948310773 80-81 21.84421483749361 29.162833307642472 27.211208202023844 21.78174365284007 82-83 21.824130742203256 29.269654317446413 26.911700001502826 21.9945149388475 84-85 21.790092318574363 29.271365056252584 26.74234330289429 22.196199322278762 86-87 21.78130813772208 29.241947046674305 26.666826752505113 22.309918063098504 88-89 21.79218028929787 29.2110591654278 26.529055263819394 22.467705281454933 90-91 21.804200379535683 29.119708698640007 26.436994055013646 22.639096866810664 92-93 21.777252894089465 29.07451606265897 26.436637842181256 22.71159320107031 94-95 21.80504178116529 29.003966937644808 26.38502380246002 22.80596747872988 96-97 21.827681117851565 28.839842516907293 26.389329687193037 22.943146678048112 98-99 21.79673692797887 28.74399330515042 26.43709307763144 23.022176689239267 100 21.737906467337513 28.677486866078024 26.404574639109125 23.180032027475345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22541.0 1 17265.0 2 9999.5 3 7312.5 4 6631.0 5 6739.5 6 6978.0 7 7171.0 8 7275.5 9 7502.5 10 8431.5 11 16183.0 12 115423.5 13 135405.0 14 61776.0 15 64690.5 16 74244.0 17 83409.0 18 91255.5 19 99914.5 20 110410.0 21 126279.0 22 149749.5 23 177069.0 24 204631.0 25 235190.5 26 271191.5 27 314335.5 28 367128.5 29 421847.0 30 467592.0 31 510226.5 32 557094.0 33 597827.5 34 633586.5 35 677623.0 36 725060.5 37 768010.0 38 813682.5 39 849807.0 40 870621.0 41 898461.0 42 934056.5 43 950275.0 44 955512.5 45 969973.0 46 981813.0 47 987110.5 48 982869.0 49 977119.5 50 976397.5 51 969137.5 52 949093.0 53 905182.0 54 842206.0 55 778667.0 56 714276.0 57 641942.5 58 562658.0 59 478393.0 60 392044.0 61 314104.5 62 244662.5 63 182354.0 64 133791.0 65 97873.0 66 70749.5 67 51978.5 68 38472.5 69 28808.0 70 22289.0 71 17608.5 72 14322.5 73 11284.0 74 8698.0 75 7058.5 76 5290.5 77 3307.5 78 1976.0 79 1230.5 80 766.0 81 504.0 82 310.5 83 172.0 84 115.5 85 76.5 86 47.0 87 32.0 88 26.0 89 21.0 90 11.5 91 8.0 92 5.5 93 3.5 94 2.5 95 2.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32819098231232735 2 0.006546732154363539 3 2.370094166319852 4 8.40482324326778E-4 5 2.7784539647166214E-5 6 0.008658357167548172 7 0.007751886561559374 8 2.6395312664807905E-4 9 0.0 10-11 7.293441657381131E-5 12-13 3.4730674558957768E-6 14-15 0.0 16-17 4.1676809470749326E-5 18-19 0.0 20-21 2.8826459883934946E-4 22-23 3.473067455895777E-5 24-25 5.192235846564186E-4 26-27 0.00342965411269708 28-29 0.006100442986280932 30-31 0.005727088234772136 32-33 0.015349221621331385 34-35 0.009804469427993777 36-37 0.0051088822276226875 38-39 0.005303374005152852 40-41 0.008399613642083937 42-43 0.019145284350625468 44-45 0.03426875658732363 46-47 0.033115698191966234 48-49 0.04001321015937524 50-51 0.04022333074045694 52-53 0.06227036295048333 54-55 0.033534202820401676 56-57 0.022356135213601115 58-59 0.016993719061698036 60-61 0.018259652149372047 62-63 0.024974828075346532 64-65 0.044941492879291355 66-67 0.04806204398841371 68-69 0.05097594758391026 70-71 0.06151323424509805 72-73 0.06890565832497221 74-75 0.059677718094657134 76-77 0.05987394640591525 78-79 0.05459141080549777 80-81 0.05182858564433268 82-83 0.04832252404760589 84-85 0.0503316935708416 86-87 0.05821729322945296 88-89 0.06496025369507462 90-91 0.06101311253144907 92-93 0.06524330869273012 94-95 0.07354220337859307 96-97 0.0729813029844659 98-99 0.0481124034665242 100 0.00724481871299859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8792991E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.690675871229644 #Duplication Level Percentage of deduplicated Percentage of total 1 66.68474719664582 26.467626865369592 2 16.566775333514013 13.150930199879744 3 6.218039908392437 7.403946196751241 4 2.9897564675138777 4.7466181954402344 5 1.7545208211465486 3.4819058610725677 6 1.14150433545186 2.7184247150413907 7 0.8156520705615766 2.266164736944981 8 0.5805691818450008 1.843454657394793 9 0.4473502862670825 1.5980071691815712 >10 2.4911781675200864 17.715632546931413 >50 0.17907830929897595 5.047455196622722 >100 0.12968741944716064 7.811162962825649 >500 5.602920920185986E-4 0.14239529521974903 >1k 5.188764252077546E-4 0.4026911360739227 >5k 2.6285947864628956E-5 0.08534599328897151 >10k+ 3.504793048583443E-5 5.118238271961588 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1377680 4.784775572638494 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36380 0.12635019404548836 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 29685 0.10309800742826614 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014065923196377897 1.3892269823583107E-5 0.0 0.0 1.7365337279478886E-5 2 0.0014204845894613728 2.083840473537466E-5 0.0 0.0 1.7365337279478886E-5 3 0.0014378499267408515 3.125760710306199E-5 0.0 6.9461349117915535E-6 2.4311472191270437E-5 4 0.001476053668755705 4.51498769266451E-5 3.4730674558957768E-6 6.9461349117915535E-6 2.4311472191270437E-5 5 0.0014864728711233926 6.598828166201976E-5 3.4730674558957768E-6 6.9461349117915535E-6 3.473067455895777E-5 6 0.0014899459385792882 6.946134911791554E-5 3.4730674558957768E-6 6.9461349117915535E-6 3.473067455895777E-5 7 0.0015142574107705588 7.293441657381131E-5 3.4730674558957768E-6 1.041920236768733E-5 4.167680947074932E-5 8 0.0015350958155059334 9.02997538532902E-5 3.4730674558957768E-6 1.041920236768733E-5 4.167680947074932E-5 9 0.0015420419504177248 9.724588876508175E-5 3.4730674558957768E-6 1.3892269823583107E-5 4.167680947074932E-5 10-11 0.001569826490064891 1.0245548994892542E-4 3.4730674558957768E-6 1.5628803551530996E-5 4.167680947074932E-5 12-13 0.0015976110297120576 1.059285574048212E-4 3.4730674558957768E-6 1.7365337279478886E-5 4.341334319869721E-5 14-15 0.0016201859681753798 1.1461122604456064E-4 3.4730674558957768E-6 3.125760710306199E-5 5.2096011838436655E-5 16-17 0.001667072378829973 1.3197656332403953E-4 3.4730674558957768E-6 3.473067455895777E-5 5.556907929433243E-5 18-19 0.0017452163965876277 1.4239576569172684E-4 3.4730674558957768E-6 3.8203742014853546E-5 5.556907929433243E-5 20-21 0.001819887346889387 1.649707041550494E-4 5.209601183843665E-6 4.6886410654592984E-5 5.556907929433243E-5 22-23 0.00195707351139727 1.7712644025068462E-4 6.9461349117915535E-6 5.904214675022821E-5 6.598828166201976E-5 24-25 0.002337374397817858 1.9970137871400717E-4 6.9461349117915535E-6 6.251521420612398E-5 7.988055148560287E-5 26-27 0.00379953579674998 2.1359364853759026E-4 6.9461349117915535E-6 6.598828166201976E-5 7.988055148560287E-5 28-29 0.008375302169892665 2.222763171773297E-4 6.9461349117915535E-6 6.598828166201976E-5 7.988055148560287E-5 30-31 0.019575944715156546 2.4658778936860015E-4 6.9461349117915535E-6 7.119788284586342E-5 8.509015266944653E-5 32-33 0.042974000165526396 2.656896603760269E-4 6.9461349117915535E-6 7.293441657381131E-5 8.856322012534231E-5 34-35 0.08567710106949292 2.865280651114016E-4 6.9461349117915535E-6 7.293441657381131E-5 9.203628758123809E-5 36-37 0.15489880853295165 2.9347420002319316E-4 6.9461349117915535E-6 7.46709503017592E-5 9.377282130918598E-5 38-39 0.2554336921787667 3.0389340239088045E-4 6.9461349117915535E-6 8.335361894149865E-5 1.0071895622097753E-4 40-41 0.3909614669764596 3.160491384865157E-4 6.9461349117915535E-6 9.203628758123809E-5 1.0071895622097753E-4 42-43 0.5618780626160027 3.455702118616298E-4 6.9461349117915535E-6 1.0071895622097753E-4 1.0245548994892542E-4 44-45 0.7762114745216987 3.559894142293171E-4 6.9461349117915535E-6 1.0766509113276908E-4 1.0419202367687331E-4 46-47 1.0415816126917832 3.8030088642058757E-4 6.9461349117915535E-6 1.1113815858866486E-4 1.0940162486071697E-4 48-49 1.3602442344388606 3.9592968997211855E-4 6.9461349117915535E-6 1.1113815858866486E-4 1.1461122604456064E-4 50-51 1.7284223789046438 4.0808542606775376E-4 6.9461349117915535E-6 1.1113815858866486E-4 1.2155736095635218E-4 52-53 2.1357142090587256 4.1329502725159746E-4 6.9461349117915535E-6 1.1808429350045642E-4 1.2329389468430008E-4 54-55 2.5867076470103436 4.3239689825902417E-4 6.9461349117915535E-6 1.2155736095635218E-4 1.2503042841224797E-4 56-57 3.094676756575932 4.480257018105552E-4 6.9461349117915535E-6 1.2503042841224797E-4 1.3024002959609164E-4 58-59 3.6594843515909825 4.688641065459299E-4 6.9461349117915535E-6 1.2503042841224797E-4 1.3892269823583106E-4 60-61 4.27474693407156 4.7581024145772145E-4 6.9461349117915535E-6 1.2850349586814375E-4 1.5455150178736207E-4 62-63 4.9244050401016 4.897025112813045E-4 6.9461349117915535E-6 1.4586883314762262E-4 1.649707041550494E-4 64-65 5.606433871354317 5.001217136489919E-4 6.9461349117915535E-6 1.4586883314762262E-4 1.6844377161094517E-4 66-67 6.329790468798466 5.157505172005228E-4 6.9461349117915535E-6 1.476053668755705E-4 1.788629739786325E-4 68-69 7.096315558185671 5.296427870241059E-4 6.9461349117915535E-6 1.5976110297120574E-4 1.823360414345283E-4 70-71 7.896986110265516 5.470081243035849E-4 6.9461349117915535E-6 1.5976110297120574E-4 1.8754564261837196E-4 72-73 8.727860193475557 5.678465290389594E-4 6.9461349117915535E-6 1.5976110297120574E-4 1.9796484498605928E-4 74-75 9.577061653650361 5.956310686861257E-4 6.9461349117915535E-6 1.5976110297120574E-4 2.0143791244195506E-4 76-77 10.452007226341994 6.129964059656046E-4 8.682668639739441E-6 1.667072378829973E-4 2.1012058108169448E-4 78-79 11.350824928191726 6.199425408773961E-4 1.041920236768733E-5 1.7018030533889306E-4 2.1880324972143393E-4 80-81 12.269326239847747 6.251521420612399E-4 1.041920236768733E-5 1.805995077065804E-4 2.1880324972143393E-4 82-83 13.206075047917043 6.477270805245623E-4 1.041920236768733E-5 1.8407257516247618E-4 2.222763171773297E-4 84-85 14.155863487749501 6.703020189878848E-4 1.041920236768733E-5 1.9101871007426772E-4 2.257493846332255E-4 86-87 15.121157437238805 6.946134911791555E-4 1.041920236768733E-5 1.9101871007426772E-4 2.3269551954501706E-4 88 15.849951121785159 7.119788284586342E-4 1.041920236768733E-5 1.944917775301635E-4 2.4658778936860015E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 185085 0.0 84.6781 8 AGTGGTA 186075 0.0 83.6099 5 TGGTATC 187895 0.0 83.38403 7 GTGGTAT 186885 0.0 83.23496 6 GTATCAA 188765 0.0 82.92242 9 AAGCAGT 183490 0.0 82.50187 1 GCAGTGG 189790 0.0 81.89936 3 AGCAGTG 196800 0.0 78.75029 2 CAGTGGT 197835 0.0 78.512245 4 CAACGCA 182330 0.0 44.23102 12-13 ATCAACG 183375 0.0 44.065998 10-11 ACGCAGA 185160 0.0 43.67794 14-15 AGAGTAC 187355 0.0 43.079063 18-19 TCAACGC 181600 0.0 43.006588 12-13 AACGCAG 183670 0.0 42.625397 14-15 GAGTACT 181885 0.0 42.607548 20-21 CGCAGAG 186675 0.0 41.944267 16-17 AGTACTT 191370 0.0 41.841206 20-21 TATCAAC 188585 0.0 41.426113 10-11 GTACTTT 192735 0.0 40.152355 22-23 >>END_MODULE