FastQCFastQC Report
Thu 26 May 2016
SRR1660740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35674813
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16320044.574667286973585No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT497660.13949897929387886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2088000.085.0616158
TGGTATC2118900.083.620457
AGTGGTA2128850.083.6027765
GTGGTAT2134050.083.3943566
GTATCAA2139250.082.9939
AAGCAGT2180600.081.926371
GCAGTGG2221750.080.566023
CAGTGGT2276850.078.075244
AGCAGTG2317650.077.271352
CAACGCA2057650.044.84478812-13
ATCAACG2074000.044.58687210-11
ACGCAGA2094100.044.15585314-15
TCAACGC2053500.043.4165112-13
AGAGTAC2099750.043.3538218-19
AACGCAG2079600.042.9569314-15
GAGTACT2060750.042.3423720-21
CGCAGAG2111300.042.25908716-17
TATCAAC2133900.041.56649810-11
AGTACTT2181100.041.39069720-21
GTACTTT2182400.039.9501122-23
Produced by FastQC (version 0.11.5)