FastQCFastQC Report
Thu 26 May 2016
SRR1660740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35674813
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15599584.372715282347801No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT540070.1513869182720033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2114750.083.77498
AAGCAGT2065850.082.8124161
AGTGGTA2128800.082.521495
TGGTATC2145050.082.517627
GTGGTAT2129800.082.40786
GTATCAA2160050.081.917819
GCAGTGG2156000.081.361073
AGCAGTG2252500.077.6063842
CAGTGGT2279350.076.907084
CAACGCA2063600.044.6315712-13
ATCAACG2081950.044.32277310-11
ACGCAGA2102800.043.89008714-15
AGAGTAC2142550.043.0376218-19
TCAACGC2058950.042.89492812-13
AACGCAG2085000.042.4525414-15
GAGTACT2097900.041.85335520-21
CGCAGAG2118300.041.78745716-17
AGTACTT2225000.041.12707520-21
TATCAAC2151250.041.082910-11
GTACTTT2208300.039.73724722-23
Produced by FastQC (version 0.11.5)