FastQCFastQC Report
Thu 26 May 2016
SRR1660739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30346078
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14439184.758170067314794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA482980.15915730527022307No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT338190.11144438500421702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1873450.083.947098
AGTGGTA1890350.083.473675
TGGTATC1892200.082.9586267
GTGGTAT1901300.082.935936
GTATCAA1915400.081.984799
AAGCAGT1945250.081.579481
GCAGTGG1971600.080.601833
CAGTGGT1972550.079.973484
AGCAGTG2070350.076.7535862
CAACGCA1852000.043.75216312-13
ATCAACG1864650.043.54459410-11
ACGCAGA1877700.043.20123314-15
AGAGTAC1865150.042.92417518-19
TCAACGC1847400.042.59015312-13
GAGTACT1812000.042.5297220-21
AACGCAG1866900.042.2235414-15
AGTACTT1894350.041.88804620-21
CGCAGAG1889550.041.6607316-17
TATCAAC1911350.040.9929710-11
GTACTTT1921800.040.0837922-23
Produced by FastQC (version 0.11.5)