##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660739_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30346078 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.871081000978116 34.0 31.0 34.0 30.0 34.0 2 31.990036373069362 34.0 31.0 34.0 30.0 34.0 3 32.05199587900618 34.0 31.0 34.0 30.0 34.0 4 35.4602367396538 37.0 35.0 37.0 33.0 37.0 5 35.434456571290696 37.0 35.0 37.0 33.0 37.0 6 35.50514102679101 37.0 35.0 37.0 33.0 37.0 7 35.468011714726366 37.0 35.0 37.0 33.0 37.0 8 35.46842409091547 37.0 35.0 37.0 33.0 37.0 9 37.152227052207536 39.0 37.0 39.0 34.0 39.0 10-11 37.101102620246344 39.0 37.0 39.0 33.0 39.0 12-13 37.108522903684616 39.0 37.0 39.0 33.0 39.0 14-15 38.47927063260036 40.0 38.0 41.0 34.0 41.0 16-17 38.45715499050652 40.0 38.0 41.0 34.0 41.0 18-19 38.419747751258 40.0 38.0 41.0 33.5 41.0 20-21 38.3041920771442 40.0 38.0 41.0 33.5 41.0 22-23 38.142345215088426 40.0 38.0 41.0 33.0 41.0 24-25 38.11767318003994 40.0 38.0 41.0 33.0 41.0 26-27 38.08731566563561 40.0 38.0 41.0 33.0 41.0 28-29 37.9967373708062 40.0 38.0 41.0 33.0 41.0 30-31 37.90126732357308 40.0 38.0 41.0 32.5 41.0 32-33 37.771230651288775 40.0 38.0 41.0 32.0 41.0 34-35 37.61077686876044 40.0 37.5 41.0 31.5 41.0 36-37 37.42243707737126 40.0 37.0 41.0 31.0 41.0 38-39 37.03324882708071 40.0 35.5 41.0 31.0 41.0 40-41 36.971048285053506 39.5 35.0 41.0 30.5 41.0 42-43 36.982683066984805 40.0 35.0 41.0 30.5 41.0 44-45 36.980310602246526 40.0 35.0 41.0 30.5 41.0 46-47 37.06862888509019 40.0 35.5 41.0 31.0 41.0 48-49 36.96010131852953 40.0 35.0 41.0 30.0 41.0 50-51 35.59607360133985 38.5 34.0 40.0 28.0 40.5 52-53 35.76856707809161 38.5 35.0 39.5 28.5 40.5 54-55 36.21442889588566 39.0 35.0 41.0 27.5 41.0 56-57 35.89775115255421 39.0 35.0 41.0 26.0 41.0 58-59 35.70981147876836 39.0 35.0 41.0 26.0 41.0 60-61 35.47760618357337 39.0 35.0 41.0 26.0 41.0 62-63 35.24349873153295 38.5 35.0 41.0 25.0 41.0 64-65 34.986316304202475 38.0 34.5 40.5 25.0 41.0 66-67 34.712073418515565 37.0 34.0 40.0 25.0 41.0 68-69 34.42599266040244 37.0 34.0 40.0 24.5 41.0 70-71 34.11557137630767 36.0 34.0 39.0 24.0 41.0 72-73 33.77004092588176 36.0 34.0 39.0 24.0 41.0 74-75 33.38891912160774 35.0 33.0 38.5 23.5 40.5 76-77 32.998166089205995 35.0 33.0 37.0 22.5 39.5 78-79 32.62854619961104 35.0 33.0 37.0 21.5 39.0 80-81 32.307319894847694 35.0 33.0 36.5 20.0 39.0 82-83 31.995047794973704 35.0 32.0 36.0 20.0 37.5 84-85 31.70979426072786 35.0 32.0 35.5 20.0 37.0 86-87 31.493737675095936 35.0 32.0 35.0 19.5 37.0 88-89 31.285057742881964 35.0 32.0 35.0 19.0 36.0 90-91 31.111271067714256 35.0 31.5 35.0 18.5 36.0 92-93 30.959007997672714 35.0 31.0 35.0 18.0 36.0 94-95 30.77860402586456 35.0 31.0 35.0 18.0 35.5 96-97 30.64305731699497 35.0 31.0 35.0 17.0 35.0 98-99 30.536697345205532 34.5 31.0 35.0 17.0 35.0 100 30.448495815505385 34.0 31.0 35.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5280250220309632 1101 2 0.5383002917891204 1101 3 0.5402235828202677 1101 4 0.4426876752063791 1101 5 0.43598991514985386 1101 6 0.4201041211670855 1101 7 0.454056040651011 1101 8 0.46312516776009716 1101 9 0.5553217665112413 1101 10-11 0.5535038034155804 1101 12-13 0.5522057587190474 1101 14-15 0.6821665407138156 1101 16-17 0.6471758662126277 1101 18-19 0.6707222285213064 1101 20-21 0.6854343940595911 1101 22-23 0.7158796835274899 1101 24-25 0.7276945651731239 1101 26-27 0.7094163519031085 1101 28-29 0.692647431125593 1101 30-31 0.6739426411360583 1101 32-33 0.6689567090784081 1101 34-35 0.6776364355636844 1101 36-37 0.6271774649618891 1101 38-39 0.6232740790763174 1101 40-41 0.6084895710655118 1101 42-43 0.6262207427641968 1101 44-45 0.6619423669056559 1101 46-47 0.6819409892840866 1101 48-49 0.7282592761575302 1101 50-51 0.6649515499829803 1101 52-53 0.6720860353678333 1101 54-55 0.6935508269758799 1101 56-57 0.6802920311051608 1101 58-59 0.615903816308105 1101 60-61 0.6228305974508359 1101 62-63 0.5992794048694066 1101 64-65 0.5638326619174805 1101 66-67 0.5591785949677757 1101 68-69 0.5317888766527616 1101 70-71 0.4803775288381402 1101 72-73 0.4772482013760211 1101 74-75 0.4439740564601635 1101 76-77 0.46685924239967136 1101 78-79 0.4779374126868632 1101 80-81 0.38159882325109606 1101 82-83 0.4379543215615058 1101 84-85 0.3946264425595949 1101 86-87 0.3404951541293286 1101 88-89 0.3584694734832752 1101 90-91 0.32662627102437014 1101 92-93 0.3179619059075769 1101 94-95 0.3521587089563383 1101 96-97 0.3445825157286073 1101 98-99 0.29694340968605815 1101 100 0.2963501205128445 1102 1 0.46286720969211004 1102 2 0.47564693936721625 1102 3 0.47751498701514095 1102 4 0.3975707731781526 1102 5 0.3959720818784618 1102 6 0.36800772871037424 1102 7 0.39821090776001 1102 8 0.42083073175972174 1102 9 0.5047487213982009 1102 10-11 0.4746679549393491 1102 12-13 0.486510937561512 1102 14-15 0.5654076677301347 1102 16-17 0.542731176528541 1102 18-19 0.511680824861422 1102 20-21 0.5307571967435578 1102 22-23 0.5709677329105176 1102 24-25 0.5263993660364576 1102 26-27 0.5171525099067082 1102 28-29 0.5602241404361905 1102 30-31 0.5265583841868349 1102 32-33 0.5258613694374716 1102 34-35 0.5330594758193143 1102 36-37 0.5524150557988889 1102 38-39 0.5463036450329497 1102 40-41 0.5179226967281352 1102 42-43 0.5438437962700746 1102 44-45 0.5660932911253482 1102 46-47 0.5995403660974645 1102 48-49 0.6263928058178578 1102 50-51 0.5821041020640294 1102 52-53 0.5933527761247959 1102 54-55 0.643523033151034 1102 56-57 0.6514704117518093 1102 58-59 0.5697534718626258 1102 60-61 0.5448492657559711 1102 62-63 0.5187956929218203 1102 64-65 0.5224602070392734 1102 66-67 0.4966268572504191 1102 68-69 0.4921639199158889 1102 70-71 0.49999031381271664 1102 72-73 0.4482099863336657 1102 74-75 0.4237734825244175 1102 76-77 0.4409340988315975 1102 78-79 0.4372820133060529 1102 80-81 0.3987277473660882 1102 82-83 0.4127803051555219 1102 84-85 0.3523397893927971 1102 86-87 0.33668789509184194 1102 88-89 0.30368306633472386 1102 90-91 0.3057777460536819 1102 92-93 0.2846783209219801 1102 94-95 0.33730657845738676 1102 96-97 0.3209544726427751 1102 98-99 0.27358250298567555 1102 100 0.24154856882078235 1103 1 0.4074865397463334 1103 2 0.4525067851382758 1103 3 0.4350890705306867 1103 4 0.3825789630269014 1103 5 0.3828807382516004 1103 6 0.39220963383122154 1103 7 0.37414665476128306 1103 8 0.414628484819886 1103 9 0.4615989710431663 1103 10-11 0.4576963918541779 1103 12-13 0.4940104124567384 1103 14-15 0.5922024091637184 1103 16-17 0.5560056650042497 1103 18-19 0.5351569291304159 1103 20-21 0.5581807985435674 1103 22-23 0.5956623782661268 1103 24-25 0.6273076351016869 1103 26-27 0.5817568669521336 1103 28-29 0.6025759804974982 1103 30-31 0.5741581066556805 1103 32-33 0.5010739369589032 1103 34-35 0.531761767164781 1103 36-37 0.5251572792043646 1103 38-39 0.5269772663664085 1103 40-41 0.5185313433529046 1103 42-43 0.5154895845504299 1103 44-45 0.5391454565381579 1103 46-47 0.5983550122966648 1103 48-49 0.6372921727920939 1103 50-51 0.563133497134892 1103 52-53 0.5682639711866457 1103 54-55 0.6185425668291131 1103 56-57 0.672315871311362 1103 58-59 0.5905521718132576 1103 60-61 0.5819751062667962 1103 62-63 0.5482115046959208 1103 64-65 0.5601330801245297 1103 66-67 0.5022219826996874 1103 68-69 0.5218846800636214 1103 70-71 0.5119910303180149 1103 72-73 0.43842526848731467 1103 74-75 0.4569010275654435 1103 76-77 0.46485761058774955 1103 78-79 0.47652399656156064 1103 80-81 0.3978405451120821 1103 82-83 0.3716627991663728 1103 84-85 0.3499724626830947 1103 86-87 0.3407131051426653 1103 88-89 0.3708784138386534 1103 90-91 0.34239202059733387 1103 92-93 0.29284419337767886 1103 94-95 0.3930165577864706 1103 96-97 0.3845880657733467 1103 98-99 0.3213081556785866 1103 100 0.33438338536312173 1104 1 0.41496951311479435 1104 2 0.44167750881037904 1104 3 0.41337458034825403 1104 4 0.35775995996448984 1104 5 0.3639072166486059 1104 6 0.29998777413343447 1104 7 0.3252415912966242 1104 8 0.32748493844628257 1104 9 0.417761940500057 1104 10-11 0.37789829524739815 1104 12-13 0.3805060868919057 1104 14-15 0.4804135341645974 1104 16-17 0.451395435241416 1104 18-19 0.4270470715256778 1104 20-21 0.400149019327948 1104 22-23 0.4494604126233739 1104 24-25 0.4775747382092277 1104 26-27 0.43709094814470717 1104 28-29 0.4064785350462685 1104 30-31 0.365374724452586 1104 32-33 0.32406221638258614 1104 34-35 0.364895787437419 1104 36-37 0.37404026312600536 1104 38-39 0.37143180061512027 1104 40-41 0.3625412633020275 1104 42-43 0.37327216459431156 1104 44-45 0.38633490185420527 1104 46-47 0.41705947587097825 1104 48-49 0.4401596683025062 1104 50-51 0.3821221377776922 1104 52-53 0.38373978293541455 1104 54-55 0.4838921498792459 1104 56-57 0.5123025306635256 1104 58-59 0.46075709432155065 1104 60-61 0.4779387649881741 1104 62-63 0.4277671771870928 1104 64-65 0.4125438428375503 1104 66-67 0.41783490632357 1104 68-69 0.4158383662386669 1104 70-71 0.4060151275392698 1104 72-73 0.36331045814583973 1104 74-75 0.34562376032688036 1104 76-77 0.36716304638615327 1104 78-79 0.349819758064406 1104 80-81 0.34555563304371617 1104 82-83 0.36903231053386776 1104 84-85 0.3188294474650455 1104 86-87 0.3157361835548933 1104 88-89 0.28743445700703774 1104 90-91 0.2651915269604963 1104 92-93 0.2826628992923581 1104 94-95 0.331873122292631 1104 96-97 0.28788872068263416 1104 98-99 0.3137499472299936 1104 100 0.26151086538567014 1105 1 0.26260360040088315 1105 2 0.30339194514837686 1105 3 0.28384614725479196 1105 4 0.257350205279522 1105 5 0.23530530239709435 1105 6 0.2121828381267079 1105 7 0.22944874413900607 1105 8 0.23899914256693933 1105 9 0.2510620883258028 1105 10-11 0.27461040053518815 1105 12-13 0.27116730031901426 1105 14-15 0.29781381452598055 1105 16-17 0.27804733629120193 1105 18-19 0.29756350983884516 1105 20-21 0.3245272129443535 1105 22-23 0.36532308476783015 1105 24-25 0.37898577146663115 1105 26-27 0.3719221762658691 1105 28-29 0.36418920170485336 1105 30-31 0.3824095135287635 1105 32-33 0.3357469098897141 1105 34-35 0.3858468435642237 1105 36-37 0.34104856846697373 1105 38-39 0.33842173689041033 1105 40-41 0.3388946710450327 1105 42-43 0.4083621127874082 1105 44-45 0.40257952985744083 1105 46-47 0.3980613545083287 1105 48-49 0.4013311635787602 1105 50-51 0.28258286970990554 1105 52-53 0.31862748048352074 1105 54-55 0.4030128184188797 1105 56-57 0.45174214711543215 1105 58-59 0.38328280645410473 1105 60-61 0.3579358955744354 1105 62-63 0.33012200107329903 1105 64-65 0.34634457952333975 1105 66-67 0.32987951885918676 1105 68-69 0.3095558667577123 1105 70-71 0.34187257237622504 1105 72-73 0.3265960435364619 1105 74-75 0.3236122345021286 1105 76-77 0.32359591310581237 1105 78-79 0.3035434308524998 1105 80-81 0.27366019412571774 1105 82-83 0.2707078616242171 1105 84-85 0.27375940180615643 1105 86-87 0.20679638505396625 1105 88-89 0.24889797374877531 1105 90-91 0.27757403063556296 1105 92-93 0.24832089487861353 1105 94-95 0.2692396220342843 1105 96-97 0.25718594046616516 1105 98-99 0.21772365975319374 1105 100 0.2422148522143921 1106 1 0.2451344698103064 1106 2 0.2922484796018736 1106 3 0.27145701087265905 1106 4 0.2529820106017837 1106 5 0.26615228563952087 1106 6 0.21979743290544462 1106 7 0.25438525870016804 1106 8 0.27236475151674 1106 9 0.2669792243191864 1106 10-11 0.26492694570779207 1106 12-13 0.26202375449678783 1106 14-15 0.33643609224990456 1106 16-17 0.3136927203323552 1106 18-19 0.26797195096860804 1106 20-21 0.30232148608276077 1106 22-23 0.2979568614916346 1106 24-25 0.2614536573587998 1106 26-27 0.2967441974361549 1106 28-29 0.3144254350080189 1106 30-31 0.2905560093834012 1106 32-33 0.3029815796267954 1106 34-35 0.3194487478596386 1106 36-37 0.2928594586375439 1106 38-39 0.30153596751780043 1106 40-41 0.30492992969903554 1106 42-43 0.31932558288058743 1106 44-45 0.3231669721142012 1106 46-47 0.31236174445201215 1106 48-49 0.32635058649499626 1106 50-51 0.23145686985085234 1106 52-53 0.2687034039081624 1106 54-55 0.3903773045797152 1106 56-57 0.41395782129100667 1106 58-59 0.31419313607364785 1106 60-61 0.2932365269931907 1106 62-63 0.3178636857999564 1106 64-65 0.25500682260125274 1106 66-67 0.28484533464740025 1106 68-69 0.25135379020293414 1106 70-71 0.279677083287325 1106 72-73 0.2837522389952696 1106 74-75 0.2773572275468794 1106 76-77 0.30007330995528747 1106 78-79 0.2596195685499225 1106 80-81 0.23767382612913224 1106 82-83 0.2854534702533016 1106 84-85 0.28345367837392743 1106 86-87 0.22298462824617005 1106 88-89 0.24504138830089772 1106 90-91 0.23656852676515072 1106 92-93 0.22017767030205349 1106 94-95 0.2247060002022181 1106 96-97 0.22758310658575454 1106 98-99 0.20542366256932354 1106 100 0.20229498504530952 1107 1 0.17038506859810454 1107 2 0.19033220539588314 1107 3 0.20283264488776354 1107 4 0.18536218235880142 1107 5 0.20038034631827628 1107 6 0.16520324356343963 1107 7 0.20003628824038344 1107 8 0.18214603136971164 1107 9 0.18793989839440428 1107 10-11 0.1946672311995954 1107 12-13 0.17057698105656272 1107 14-15 0.22588113787492148 1107 16-17 0.2139134697662044 1107 18-19 0.17531583511154025 1107 20-21 0.18329545944492764 1107 22-23 0.2146583084650615 1107 24-25 0.24609840862778753 1107 26-27 0.2186173620943137 1107 28-29 0.2206004332315814 1107 30-31 0.17168532141869974 1107 32-33 0.1641655085426592 1107 34-35 0.19625803301758538 1107 36-37 0.16986658919503128 1107 38-39 0.19047083431275524 1107 40-41 0.1799152621887643 1107 42-43 0.22025611750799357 1107 44-45 0.25240908919290206 1107 46-47 0.24123352624429373 1107 48-49 0.2603228540497753 1107 50-51 0.19650479890835726 1107 52-53 0.18082256217117987 1107 54-55 0.2658867395251505 1107 56-57 0.26590634346654696 1107 58-59 0.2318396641501863 1107 60-61 0.28749885816187515 1107 62-63 0.22843441205150583 1107 64-65 0.22771260535549231 1107 66-67 0.24050072687853685 1107 68-69 0.2199053321241209 1107 70-71 0.2019429158410233 1107 72-73 0.1936544772799209 1107 74-75 0.246227889629985 1107 76-77 0.21498918177171333 1107 78-79 0.24251808300648037 1107 80-81 0.20734898653899592 1107 82-83 0.24058809757136146 1107 84-85 0.2342687913718713 1107 86-87 0.21173443684495297 1107 88-89 0.21025090821479253 1107 90-91 0.21856088232759774 1107 92-93 0.21795740093979887 1107 94-95 0.23741984867884725 1107 96-97 0.2535977492327639 1107 98-99 0.24748879976994687 1107 100 0.233476859232578 1108 1 0.14215572377295516 1108 2 0.17340875058406624 1108 3 0.20360250259741264 1108 4 0.17469956544978515 1108 5 0.1852678145754325 1108 6 0.14155232261084194 1108 7 0.14706246005975032 1108 8 0.18376936904351737 1108 9 0.17679047168935824 1108 10-11 0.16394031234768391 1108 12-13 0.18115868741266894 1108 14-15 0.2274613554318634 1108 16-17 0.16864301414509697 1108 18-19 0.1565391571849446 1108 20-21 0.1566511354500122 1108 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-1.353283877452263 2316 76-77 -1.3355101527769513 2316 78-79 -1.0776198242322792 2316 80-81 -1.1068160684131243 2316 82-83 -1.058165561543401 2316 84-85 -1.1708563195297081 2316 86-87 -1.3779324537972286 2316 88-89 -1.3085158040676816 2316 90-91 -1.210176763720522 2316 92-93 -1.0664111560417346 2316 94-95 -1.3326672384752953 2316 96-97 -1.481176485078798 2316 98-99 -1.3352886128888635 2316 100 -1.1270126356955075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15937.0 3 3891.0 4 2799.0 5 2291.0 6 3531.0 7 15628.0 8 32343.0 9 40400.0 10 43915.0 11 41954.0 12 41038.0 13 43787.0 14 50551.0 15 61941.0 16 79596.0 17 97349.0 18 106821.0 19 118635.0 20 137497.0 21 167393.0 22 215054.0 23 311416.0 24 433278.0 25 500552.0 26 495778.0 27 442272.0 28 413944.0 29 415509.0 30 467480.0 31 567356.0 32 711654.0 33 921102.0 34 1273995.0 35 1934588.0 36 3353903.0 37 6311809.0 38 8036949.0 39 2427167.0 40 4975.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.703561967463658 33.14433963003908 26.530451135518923 24.621647266978343 2 20.238100278864827 32.09848592609255 29.690403808175404 17.97300998686722 3 24.238454163495742 26.357587169677167 27.23507454003237 22.16888412679472 4 18.489840106018292 28.31602866481684 26.155075688565155 27.039055540599715 5 21.451657016716634 32.30025816140059 26.768955112656105 19.47912970922667 6 26.754265923049854 27.56595748527027 26.666922208114297 19.012854383565582 7 20.59505490676225 26.907342759799345 32.23983436895634 20.257767964482063 8 25.370693339543553 26.70163225817544 27.33977580841444 20.58789859386657 9 25.042611940529984 25.356696606613205 28.801811034695856 20.79888041816095 10-11 20.366965248029167 29.611359944953332 29.5709717983662 20.450703008651296 12-13 20.402649524442257 27.026402884532352 29.264826822896 23.30612076812939 14-15 20.370839575757177 32.601445712362306 26.514712870502983 20.513001841377534 16-17 23.242018975474345 26.851421860334124 26.579554223075647 23.327004941115888 18-19 20.417500966422143 29.707358711558165 26.565232978902912 23.309907343116784 20-21 23.23969977202909 29.78236755470881 26.529140001725825 20.44879267153627 22-23 23.232799417765563 27.008773215566556 29.271009385639058 20.487417981028823 24-25 20.512310604342655 29.67128017124298 26.393756274294205 23.422652950120153 26-27 20.53984269663885 26.94079221754611 32.0297848181606 20.489580267654443 28-29 20.53501218453465 27.04023457797988 31.980066624468773 20.444686613016692 30-31 20.515793863093847 26.96381917209439 31.962956386249775 20.557430578561984 32-33 20.604815586967295 27.08274625379547 31.805035509951697 20.507402649285535 34-35 20.62798418352732 27.18459004453424 31.6901877135259 20.497238058412538 36-37 20.564196504858828 27.1205368555631 31.75295366946545 20.562312970112625 38-39 20.62518579594687 27.129787268011533 31.742967858439226 20.502059077602375 40-41 20.666974894231497 27.149888914046734 31.716896808155788 20.46623938356598 42-43 20.658151716338676 27.19524239243806 31.646189143535608 20.50041674768766 44-45 20.70078679217714 27.266814315575488 31.618808936084015 20.413589956163356 46-47 20.694238360003165 27.38074926943343 31.52749272995437 20.397519640609037 48-49 20.798964834953782 27.366140243048804 31.40794541154191 20.426949510455504 50-51 20.82538137958845 27.40949847316221 31.35767422567512 20.407445921574222 52-53 20.97573317793982 27.52245093797724 31.09982092689506 20.40199495718789 54-55 21.327852795493417 27.69548208933907 30.446471687554123 20.530193427613394 56-57 21.76664252344958 28.169626904824614 29.476212487404972 20.587518084320834 58-59 21.95324552093844 28.60259857287045 28.857146712069216 20.587009194121894 60-61 22.18773052180058 28.80808242214305 28.465156039157392 20.539031016898978 62-63 22.292738532041845 28.945886687636985 28.28521476410635 20.476160016214823 64-65 22.431910816055062 29.021041306672036 28.18422261028544 20.362825266987468 66-67 22.550655182482714 28.878569767077387 28.29809441952465 20.272680630915243 68-69 22.725003286800423 28.741046634389782 28.412180165530014 20.121769913279778 70-71 22.885124354228843 28.624919975890272 28.45367229928064 20.03628337060024 72-73 23.132843340991002 28.5148434464305 28.388401826887744 19.963911385690754 74-75 23.392730448430825 28.56919442308691 28.260464860642827 19.777610267839442 76-77 23.727340183033483 28.647994172894776 27.978039386669362 19.64662625740238 78-79 24.031281201736025 28.75828451710991 27.63291640569857 19.577517875455495 80-81 24.339917733213213 28.879734389581955 27.338050252741592 19.442297624463237 82-83 24.566720966974902 28.976174970692465 27.025342418291352 19.43176164404128 84-85 24.743787709741234 28.980735976567036 26.873860018616462 19.401616295075268 86-87 24.945667187079096 28.931982834704844 26.80053395397432 19.32181602424174 88-89 25.172219124763863 28.920804809437673 26.65328049878935 19.253695567009114 90-91 25.388404082875777 28.86180899522739 26.540976188944793 19.208810732952045 92-93 25.535180089042132 28.86398189456913 26.46703193893839 19.13380607745035 94-95 25.70177830605596 28.864850706381418 26.353941989960795 19.079428997601827 96-97 25.856919407347263 28.793325899085165 26.273757873866614 19.07599681970096 98-99 26.008828619696793 28.785466435255984 26.211989353841698 18.99371559120552 100 26.061401442681476 28.78059706677795 26.11655520495032 19.04144628559025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 46070.0 1 31449.5 2 14292.0 3 11035.5 4 10252.0 5 10246.5 6 10586.0 7 10765.0 8 10747.5 9 11097.0 10 11743.0 11 14469.0 12 112942.5 13 138519.0 14 65498.0 15 66872.5 16 74758.0 17 82854.5 18 90909.0 19 99236.0 20 110152.5 21 128438.0 22 156424.0 23 187422.0 24 217656.5 25 253575.0 26 294656.0 27 342500.0 28 404244.0 29 463633.5 30 510558.0 31 560308.5 32 617541.5 33 660566.0 34 696268.5 35 749348.0 36 799476.0 37 838639.5 38 885623.0 39 921263.5 40 938870.5 41 972195.0 42 1017969.0 43 1023370.5 44 1000529.5 45 998140.5 46 1004066.0 47 1006334.0 48 1003407.5 49 994331.0 50 982209.5 51 970020.0 52 953421.5 53 913718.0 54 855858.0 55 795399.5 56 731578.5 57 661509.5 58 582993.0 59 495424.5 60 404994.5 61 325907.0 62 256005.0 63 191185.0 64 140347.5 65 102063.5 66 73218.0 67 54512.5 68 41354.0 69 31489.0 70 24041.5 71 18999.0 72 15836.0 73 13032.5 74 10995.5 75 9629.5 76 7508.5 77 4965.5 78 3345.5 79 2556.5 80 2049.0 81 1726.5 82 1450.0 83 1227.5 84 1018.5 85 869.5 86 736.0 87 601.5 88 554.0 89 520.0 90 442.5 91 387.0 92 339.5 93 278.0 94 228.5 95 187.0 96 172.0 97 137.0 98 93.0 99 68.0 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4019201426952109 2 0.38350260616874443 3 0.16899383175644642 4 0.175287890580127 5 0.2742594940934377 6 0.10334449150232858 7 0.13713139470609678 8 0.14280263828492104 9 0.13574077019112651 10-11 0.13232846761943998 12-13 0.13416890314458427 14-15 0.14063761386232512 16-17 0.14504675035765743 18-19 0.15641395240597483 20-21 0.13765535038827753 22-23 0.10035728505014717 24-25 0.0953105043755572 26-27 0.08966232802802392 28-29 0.099482377920468 30-31 0.10375970166556614 32-33 0.10488834833944603 34-35 0.10540571338411509 36-37 0.10128491727992 38-39 0.10071153181640145 40-41 0.10154195214287659 42-43 0.0645058646458366 44-45 0.06766937065145619 46-47 0.08606383994663165 48-49 0.09040542240746893 50-51 0.09936374644525726 52-53 0.10427377139147931 54-55 0.10672384088645656 56-57 0.10718353785289815 58-59 0.10664310557693814 60-61 0.10446160456056298 62-63 0.10899266784986186 64-65 0.11583539724639211 66-67 0.1243257860208492 68-69 0.1293363181891248 70-71 0.12787649198028161 72-73 0.11486163055403732 74-75 0.11240991339968216 76-77 0.11411524085583645 78-79 0.11290750653181608 80-81 0.10712422211529278 82-83 0.11033221492411638 84-85 0.1162456644314959 86-87 0.12965761176782054 88-89 0.13728957000637776 90-91 0.1443646193751957 92-93 0.14357044755503495 94-95 0.13344722833705233 96-97 0.1301453189436869 98-99 0.12540665057276926 100 0.11951791595605862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0346078E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.0233062363986 #Duplication Level Percentage of deduplicated Percentage of total 1 62.86714607010844 22.646824550919185 2 18.25046220727664 13.148839780970908 3 7.531769635671225 8.139577322633782 4 3.4574775361530046 4.981990883612342 5 1.9430537757687605 3.4997610599154316 6 1.2370329814344518 2.6737210748843063 7 0.8408157433094019 2.1202274106733787 8 0.6018055209824772 1.734321966168574 9 0.4190917400223099 1.3587363082771933 >10 2.4543207815994994 16.25406101882622 >50 0.21847843705883846 5.471269019405763 >100 0.1763756389226432 12.11146923519112 >500 0.001481254519486152 0.30737622843630935 >1k 6.33840096467017E-4 0.4480653502764707 >5k 1.8279025428585323E-5 0.03602494461894235 >10k+ 3.655805079327779E-5 5.067733845190025 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1443918 4.758170067314794 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48298 0.15915730527022307 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 33819 0.11144438500421702 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0022342261164688235 6.590637511707444E-6 0.0 0.0 3.295318755853722E-5 2 0.002237521435224677 1.3181275023414888E-5 0.0 0.0 3.624850631439094E-5 3 0.0022441120727363846 1.3181275023414888E-5 0.0 0.0 5.272510009365955E-5 4 0.0022441120727363846 2.6362550046829776E-5 0.0 0.0 5.931573760536699E-5 5 0.0022441120727363846 3.954382507024466E-5 0.0 0.0 8.238296889634305E-5 6 0.0022441120727363846 4.2839143826098384E-5 0.0 0.0 8.238296889634305E-5 7 0.0022441120727363846 4.942978133780583E-5 0.0 0.0 8.567828765219677E-5 8 0.0022441120727363846 4.942978133780583E-5 0.0 0.0 8.567828765219677E-5 9 0.0022441120727363846 5.272510009365955E-5 0.0 0.0 8.567828765219677E-5 10-11 0.0022572933477597997 6.590637511707444E-5 0.0 0.0 9.72119032976848E-5 12-13 0.0022836558978066293 8.073530951841618E-5 0.0 0.0 1.054502001873191E-4 14-15 0.002316609085365166 1.0215488143146537E-4 0.0 0.0 1.1533615645488027E-4 16-17 0.002380867801104314 1.0215488143146537E-4 0.0 0.0 1.2027913458866085E-4 18-19 0.002441831198087608 1.1204083769902654E-4 0.0 1.3181275023414888E-5 1.3675572836792946E-4 20-21 0.0025027945950709014 1.2192679396658772E-4 0.0 1.4828934401341748E-5 1.4993700339134433E-4 22-23 0.002626369048415416 1.3675572836792946E-4 0.0 1.647659377926861E-5 1.5487998152512492E-4 24-25 0.003077827717967376 1.4169870650171005E-4 0.0 2.9657868802683497E-5 1.6641359717061295E-4 26-27 0.004616741576951064 1.4993700339134433E-4 0.0 3.295318755853722E-5 1.8289019094988156E-4 28-29 0.010085323052290315 1.5652764090305177E-4 0.0 3.295318755853722E-5 2.1419571913049192E-4 30-31 0.022988143640835564 1.6147061903683236E-4 0.0 3.295318755853722E-5 2.306723129097605E-4 32-33 0.04965386301320388 1.6147061903683236E-4 0.0 3.295318755853722E-5 2.586825223345172E-4 34-35 0.09724815180399918 1.6147061903683236E-4 0.0 3.460084693646408E-5 2.652731598462246E-4 36-37 0.17228750285292221 1.6147061903683236E-4 0.0 3.624850631439094E-5 2.669208192241515E-4 38-39 0.2830217466652527 1.697089159264667E-4 0.0 3.624850631439094E-5 2.702161379800052E-4 40-41 0.42974746192901764 1.7794721281610097E-4 0.0 3.78961656923178E-5 2.9987400678268866E-4 42-43 0.6142951982130936 1.9936678472915018E-4 0.0 4.613446258195211E-5 3.0975996305024984E-4 44-45 0.8435208661890343 2.1254805975256505E-4 0.0 4.613446258195211E-5 3.179982599398842E-4 46-47 1.1265524988105546 2.1749103788634564E-4 0.0 4.613446258195211E-5 3.328271943412259E-4 48-49 1.4653903545624578 2.3067231290976054E-4 0.0 4.613446258195211E-5 3.460084693646408E-4 50-51 1.855187019554883 2.4220592855524854E-4 0.0 4.613446258195211E-5 3.4765612874256767E-4 52-53 2.2832802314684617 2.553872035786634E-4 0.0 4.942978133780583E-5 3.493037881204945E-4 54-55 2.7597734376086427 2.7021613798000523E-4 0.0 4.942978133780583E-5 3.5259910687634826E-4 56-57 3.2952166009722905 2.768067754917126E-4 0.0 5.272510009365955E-5 3.575420850101288E-4 58-59 3.888359148091559 2.8010209424756636E-4 0.0 5.272510009365955E-5 3.641327225218363E-4 60-61 4.530896875701696 2.982263474047618E-4 0.0 5.931573760536699E-5 3.756663381673243E-4 62-63 5.20905205608448 3.08112303672323E-4 0.0 6.261105636122072E-5 3.8060931630110484E-4 64-65 5.920119232541351 3.2129357869573787E-4 0.0 6.261105636122072E-5 3.8719995381281233E-4 66-67 6.67272390191576 3.3612251309707964E-4 0.0 6.425871573914758E-5 4.0532420697000774E-4 68-69 7.468573039323236 3.4600846936464077E-4 0.0 6.920169387292815E-5 4.15210163237569E-4 70-71 8.296459265675123 3.5589442563220195E-4 0.0 6.920169387292815E-5 4.2015314137134956E-4 72-73 9.146315382172286 3.707233600335437E-4 0.0 7.084935325085503E-5 4.250961195051301E-4 74-75 10.016689800902771 3.7237101941147056E-4 0.0 7.249701262878188E-5 4.2839143826098384E-4 76-77 10.908225109023974 3.756663381673243E-4 0.0 7.57923313846356E-5 4.2839143826098384E-4 78-79 11.827128039412539 3.90495272568666E-4 0.0 7.57923313846356E-5 4.316867570168376E-4 80-81 12.769037237695098 3.9873356945830036E-4 0.0 7.57923313846356E-5 4.3827739452854497E-4 82-83 13.726277906489265 4.0697186634793464E-4 0.0 7.57923313846356E-5 4.432203726623256E-4 84-85 14.693277661778897 4.15210163237569E-4 0.0 8.073530951841618E-5 4.5475398830781364E-4 86-87 15.672129030973952 4.3003909763891074E-4 0.0 8.238296889634305E-5 4.5475398830781364E-4 88 16.41770972842026 4.349820757726913E-4 0.0 8.238296889634305E-5 4.679352633312285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 187345 0.0 83.94709 8 AGTGGTA 189035 0.0 83.47367 5 TGGTATC 189220 0.0 82.958626 7 GTGGTAT 190130 0.0 82.93593 6 GTATCAA 191540 0.0 81.98479 9 AAGCAGT 194525 0.0 81.57948 1 GCAGTGG 197160 0.0 80.60183 3 CAGTGGT 197255 0.0 79.97348 4 AGCAGTG 207035 0.0 76.753586 2 CAACGCA 185200 0.0 43.752163 12-13 ATCAACG 186465 0.0 43.544594 10-11 ACGCAGA 187770 0.0 43.201233 14-15 AGAGTAC 186515 0.0 42.924175 18-19 TCAACGC 184740 0.0 42.590153 12-13 GAGTACT 181200 0.0 42.52972 20-21 AACGCAG 186690 0.0 42.22354 14-15 AGTACTT 189435 0.0 41.888046 20-21 CGCAGAG 188955 0.0 41.66073 16-17 TATCAAC 191135 0.0 40.99297 10-11 GTACTTT 192180 0.0 40.08379 22-23 >>END_MODULE