FastQCFastQC Report
Thu 26 May 2016
SRR1660739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30346078
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12701304.185483211372488No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT367070.12096126557112256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365170.12033515500751035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1746550.082.276268
AGTGGTA1756350.081.195165
TGGTATC1772000.081.052787
AAGCAGT1718100.080.835871
GTGGTAT1763150.080.780836
GTATCAA1785500.080.405079
GCAGTGG1778800.080.366513
CAGTGGT1833700.077.6297154
AGCAGTG1881400.075.750982
CAACGCA1723300.043.04823712-13
ATCAACG1738750.042.7332510-11
ACGCAGA1745950.042.52097314-15
AGAGTAC1759450.042.07107518-19
GAGTACT1688050.042.0169920-21
TCAACGC1716850.041.6871112-13
AACGCAG1733950.041.33988614-15
AGTACTT1779050.041.28339420-21
CGCAGAG1758350.040.74907716-17
TATCAAC1787800.040.08527410-11
GTACTTT1803750.039.38859622-23
Produced by FastQC (version 0.11.5)