##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660739_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30346078 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.157637603119586 34.0 31.0 34.0 27.0 34.0 2 31.824592093910784 34.0 31.0 34.0 28.0 34.0 3 31.597139669910558 34.0 31.0 34.0 30.0 34.0 4 35.53576580802304 37.0 35.0 37.0 33.0 37.0 5 35.87975783888778 37.0 35.0 37.0 33.0 37.0 6 35.332751929260844 37.0 35.0 37.0 33.0 37.0 7 35.562302416806546 37.0 35.0 37.0 33.0 37.0 8 35.92287108732799 37.0 35.0 37.0 33.0 37.0 9 37.73041929833568 39.0 37.0 39.0 35.0 39.0 10-11 37.770545999387465 39.0 37.5 39.0 35.0 39.0 12-13 37.81911942624019 39.0 38.0 39.0 35.0 39.0 14-15 39.25430256588677 41.0 39.0 41.0 36.0 41.0 16-17 39.21884765471175 41.0 39.0 41.0 36.0 41.0 18-19 39.218141500855566 41.0 39.0 41.0 36.0 41.0 20-21 39.164013369371816 40.5 39.0 41.0 36.0 41.0 22-23 39.06177203525279 40.0 39.0 41.0 35.5 41.0 24-25 39.05689598504294 40.0 39.0 41.0 35.0 41.0 26-27 38.98835368445306 40.0 39.0 41.0 35.0 41.0 28-29 38.89811118260488 40.0 38.0 41.0 35.0 41.0 30-31 38.797201107833445 40.0 38.0 41.0 35.0 41.0 32-33 38.667448014204666 40.0 38.0 41.0 34.5 41.0 34-35 38.57540117704831 40.0 38.0 41.0 34.0 41.0 36-37 38.453618948715544 40.0 38.0 41.0 34.0 41.0 38-39 38.11351402642542 40.0 37.0 41.0 34.0 41.0 40-41 37.978351634105735 40.0 37.0 41.0 33.0 41.0 42-43 37.812230908389544 40.0 37.0 41.0 33.0 41.0 44-45 37.68399756634119 40.0 36.5 41.0 33.0 41.0 46-47 37.53082600987185 40.0 36.0 41.0 33.0 41.0 48-49 37.572894889415366 40.0 36.0 41.0 33.0 41.0 50-51 37.57451730665162 40.0 36.0 41.0 33.0 41.0 52-53 37.35227491341715 40.0 36.0 41.0 32.5 41.0 54-55 36.92129274498009 40.0 36.0 41.0 31.0 41.0 56-57 36.527606598783535 39.5 35.0 41.0 30.0 41.0 58-59 36.40546172062169 39.0 35.0 41.0 30.0 41.0 60-61 36.25493455859436 39.0 35.0 41.0 29.0 41.0 62-63 36.01658201102627 39.0 35.0 41.0 29.0 41.0 64-65 35.74979865602402 38.0 35.0 40.5 29.0 41.0 66-67 35.46433754964974 37.5 35.0 40.0 28.5 41.0 68-69 35.186953203639696 37.0 35.0 40.0 28.0 41.0 70-71 34.875019928440175 36.5 34.5 39.0 28.0 41.0 72-73 34.52764462676198 36.0 34.0 39.0 28.0 41.0 74-75 34.149672850639874 35.5 34.0 38.5 27.0 40.0 76-77 32.44317478192734 34.5 32.0 36.5 25.0 39.0 78-79 33.38368012499012 35.0 33.5 37.0 26.0 39.0 80-81 33.28889550405822 35.0 34.0 36.5 26.0 39.0 82-83 33.035574728964974 35.0 34.0 36.0 26.0 38.0 84-85 32.78953196192272 35.0 34.0 36.0 26.0 37.0 86-87 32.57645897436895 35.0 34.0 35.0 26.0 37.0 88-89 32.40480488121068 35.0 33.0 35.0 25.0 36.0 90-91 32.24642927497912 35.0 33.0 35.0 25.0 36.0 92-93 32.10423711097032 35.0 33.0 35.0 25.0 36.0 94-95 32.00239083614034 35.0 33.0 35.0 25.0 36.0 96-97 31.905187434765047 35.0 33.0 35.0 24.0 35.0 98-99 31.813839485287026 35.0 33.0 35.0 24.0 35.0 100 31.760106001177483 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6044718944124163 1101 2 1.2105619604053963 1101 3 1.564125728193929 1101 4 0.9503248280484229 1101 5 0.5784173856118855 1101 6 1.1231916652729055 1101 7 0.8373705855195155 1101 8 0.4961663822189024 1101 9 0.5117984481503441 1101 10-11 0.42284208648073474 1101 12-13 0.3941666040035088 1101 14-15 0.5127320687278853 1101 16-17 0.5472290614573154 1101 18-19 0.5220975490006694 1101 20-21 0.5268116332632999 1101 22-23 0.5154447897538788 1101 24-25 0.5047087071709484 1101 26-27 0.5171690750896829 1101 28-29 0.5058054437571258 1101 30-31 0.4933125972049268 1101 32-33 0.5477426178751941 1101 34-35 0.5590165504413633 1101 36-37 0.5029998596319771 1101 38-39 0.51932378922249 1101 40-41 0.5236877060587943 1101 42-43 0.5386406878108758 1101 44-45 0.48686175124139197 1101 46-47 0.5084052856364778 1101 48-49 0.4850738345474781 1101 50-51 0.46599748169384725 1101 52-53 0.4640247963119677 1101 54-55 0.44741185335904277 1101 56-57 0.4747841375607038 1101 58-59 0.4782680969909663 1101 60-61 0.49232820868341776 1101 62-63 0.41688878900964 1101 64-65 0.42244593223173155 1101 66-67 0.42542707707217886 1101 68-69 0.38955879646512415 1101 70-71 0.34962625368667233 1101 72-73 0.35517134243925597 1101 74-75 0.3832919463798987 1101 76-77 0.29570917523342644 1101 78-79 0.41690370679396693 1101 80-81 0.3868110514010965 1101 82-83 0.37416071251008276 1101 84-85 0.365999087084937 1101 86-87 0.3619434497792682 1101 88-89 0.3769492158586374 1101 90-91 0.40709047840468315 1101 92-93 0.38252444217079784 1101 94-95 0.364271517155089 1101 96-97 0.3435907371085243 1101 98-99 0.3586167507434048 1101 100 0.3718522129521453 1102 1 1.5203497362354845 1102 2 1.1532764524102213 1102 3 1.504965134992382 1102 4 0.92382158722328 1102 5 0.5152559705511521 1102 6 1.0822066759510136 1102 7 0.7788136156951779 1102 8 0.453119980158057 1102 9 0.49094144545022544 1102 10-11 0.3874928624498324 1102 12-13 0.38402884959070605 1102 14-15 0.48611842402146266 1102 16-17 0.5157503830573305 1102 18-19 0.4545147443650279 1102 20-21 0.4374183249992072 1102 22-23 0.44449554456868157 1102 24-25 0.4357055588506711 1102 26-27 0.45754156796781587 1102 28-29 0.4738232689375863 1102 30-31 0.47486432121525013 1102 32-33 0.49141727594800244 1102 34-35 0.46329001326584773 1102 36-37 0.45182680041406087 1102 38-39 0.4720051239116003 1102 40-41 0.47812752745321063 1102 42-43 0.4968746899679033 1102 44-45 0.4612401607876677 1102 46-47 0.4434082613144881 1102 48-49 0.42861448153610837 1102 50-51 0.40177420548708653 1102 52-53 0.3501985194652022 1102 54-55 0.38446755641503216 1102 56-57 0.4590799048341765 1102 58-59 0.45292593839321427 1102 60-61 0.44979968738633147 1102 62-63 0.4430235257258346 1102 64-65 0.37767839453638175 1102 66-67 0.38595990439274175 1102 68-69 0.3333658405704867 1102 70-71 0.32055199531728107 1102 72-73 0.3264688282353916 1102 74-75 0.3097404141742217 1102 76-77 0.16296630361491538 1102 78-79 0.374043740267588 1102 80-81 0.38104523844396 1102 82-83 0.38230264816649395 1102 84-85 0.3897006459660659 1102 86-87 0.36683408661755834 1102 88-89 0.3555867224715712 1102 90-91 0.346295461687383 1102 92-93 0.3330534323936476 1102 94-95 0.33259719040862024 1102 96-97 0.36768468944597643 1102 98-99 0.35426406794959675 1102 100 0.37244661514508337 1103 1 1.4979630348669382 1103 2 1.1120456377871086 1103 3 1.4344556305007998 1103 4 0.8998539737968798 1103 5 0.5082883469744601 1103 6 1.037535344458 1103 7 0.7860104223000235 1103 8 0.4440886001395157 1103 9 0.44667249494248296 1103 10-11 0.3462538728941169 1103 12-13 0.3325678910896599 1103 14-15 0.3896712235259585 1103 16-17 0.4116834787933499 1103 18-19 0.3834388818223431 1103 20-21 0.3846520001768212 1103 22-23 0.37633636633987066 1103 24-25 0.37258974192402405 1103 26-27 0.3864872652767346 1103 28-29 0.38899372182074643 1103 30-31 0.3984975213152353 1103 32-33 0.441821247278007 1103 34-35 0.41404325632506556 1103 36-37 0.39403282347647206 1103 38-39 0.41111682413994544 1103 40-41 0.4287542544601024 1103 42-43 0.474595921935169 1103 44-45 0.46433881296121626 1103 46-47 0.46974711332077135 1103 48-49 0.43426476016502136 1103 50-51 0.4135160171307035 1103 52-53 0.3951900057273079 1103 54-55 0.4229283913786901 1103 56-57 0.4751678918585469 1103 58-59 0.4721286455883771 1103 60-61 0.45202007785280074 1103 62-63 0.3874412181077176 1103 64-65 0.3687021669356838 1103 66-67 0.3698226453255842 1103 68-69 0.3467775629371985 1103 70-71 0.3525677647600034 1103 72-73 0.30980044613556146 1103 74-75 0.3623851647752119 1103 76-77 0.2177022371719346 1103 78-79 0.3661663680474234 1103 80-81 0.4034913537691551 1103 82-83 0.4020949103839726 1103 84-85 0.3879480770832018 1103 86-87 0.3613340687501818 1103 88-89 0.38347778095045726 1103 90-91 0.41707160717339775 1103 92-93 0.4360132316875749 1103 94-95 0.37421469511337335 1103 96-97 0.4170097104535948 1103 98-99 0.4377593839425167 1103 100 0.43350150345112226 1104 1 1.4552500942577744 1104 2 1.0745056920038074 1104 3 1.4239688754682973 1104 4 0.8725555831792349 1104 5 0.48009312698001594 1104 6 1.019402809697155 1104 7 0.7502462668501764 1104 8 0.3994520375456929 1104 9 0.40477119148369667 1104 10-11 0.3057887463627651 1104 12-13 0.29980219349859993 1104 14-15 0.356313366377897 1104 16-17 0.37430569640170575 1104 18-19 0.3292808402695826 1104 20-21 0.3440236646329424 1104 22-23 0.33086907603967575 1104 24-25 0.3407999814860574 1104 26-27 0.329915543693275 1104 28-29 0.30471351286006865 1104 30-31 0.35977795497735343 1104 32-33 0.35845549225421536 1104 34-35 0.3900236896124696 1104 36-37 0.3678599334948345 1104 38-39 0.37686306130594005 1104 40-41 0.362111915102318 1104 42-43 0.38205359751679424 1104 44-45 0.34264091362744153 1104 46-47 0.3615795806864952 1104 48-49 0.3073109798160516 1104 50-51 0.3030361935366912 1104 52-53 0.3299831570861116 1104 54-55 0.3133425140834447 1104 56-57 0.346761347136173 1104 58-59 0.31282634981851487 1104 60-61 0.33496056830519905 1104 62-63 0.2976423894812683 1104 64-65 0.2627804911187255 1104 66-67 0.2885059719043497 1104 68-69 0.26629455204973596 1104 70-71 0.2614961766339192 1104 72-73 0.2323236980464216 1104 74-75 0.26260046825007066 1104 76-77 0.15048706203977957 1104 78-79 0.275448893076053 1104 80-81 0.30823237376053925 1104 82-83 0.31648778088915464 1104 84-85 0.3034194463256057 1104 86-87 0.27104923341549636 1104 88-89 0.26048694127976546 1104 90-91 0.258305029409172 1104 92-93 0.27313016931388034 1104 94-95 0.2553525188769541 1104 96-97 0.23787143308479486 1104 98-99 0.2567948698182043 1104 100 0.277613932526112 1105 1 1.3568971713239826 1105 2 0.9900846797665928 1105 3 1.343346666220981 1105 4 0.8256341730387362 1105 5 0.4104672939473275 1105 6 0.9754775046191568 1105 7 0.710315520883853 1105 8 0.32748046562218036 1105 9 0.3338861701756528 1105 10-11 0.24690196746780657 1105 12-13 0.22566999802838694 1105 14-15 0.28174014699744987 1105 16-17 0.28619548611538903 1105 18-19 0.2523104071952673 1105 20-21 0.2747667493670747 1105 22-23 0.2671694066764161 1105 24-25 0.24848660748202178 1105 26-27 0.28966115766892386 1105 28-29 0.27140640872043065 1105 30-31 0.2819038887867933 1105 32-33 0.3083288267642672 1105 34-35 0.3142920324029461 1105 36-37 0.3115545020696686 1105 38-39 0.3254782417045661 1105 40-41 0.33614516126770866 1105 42-43 0.3233525460489872 1105 44-45 0.29505434278825504 1105 46-47 0.30701897447692517 1105 48-49 0.269585780083915 1105 50-51 0.28921855631115534 1105 52-53 0.2767575434339804 1105 54-55 0.28918807238609645 1105 56-57 0.306595964954802 1105 58-59 0.25935893312295377 1105 60-61 0.23331506595573615 1105 62-63 0.2163103420754524 1105 64-65 0.19481501189741124 1105 66-67 0.17926824937989494 1105 68-69 0.15378064946445846 1105 70-71 0.17655766034899756 1105 72-73 0.15480079395494073 1105 74-75 0.15647360222304485 1105 76-77 0.055521952988662804 1105 78-79 0.17336285727173362 1105 80-81 0.20228793017523117 1105 82-83 0.21164908142337424 1105 84-85 0.19105321551260346 1105 86-87 0.19358947446652763 1105 88-89 0.1779915797614109 1105 90-91 0.19813994828587767 1105 92-93 0.1818968529799747 1105 94-95 0.17212783641942764 1105 96-97 0.16489615411157743 1105 98-99 0.19503475814753912 1105 100 0.19210688116709917 1106 1 1.3630058842351822 1106 2 0.9854824734177932 1106 3 1.3254313217035971 1106 4 0.7923712775893108 1106 5 0.3986599351572835 1106 6 0.9470947132236844 1106 7 0.6830631026384921 1106 8 0.3284550420701322 1106 9 0.2965618003448611 1106 10-11 0.20969850720536698 1106 12-13 0.17849386934963007 1106 14-15 0.24237597824016888 1106 16-17 0.22866433306155187 1106 18-19 0.18992449842358639 1106 20-21 0.20635330446319244 1106 22-23 0.235213075170293 1106 24-25 0.2328350570925295 1106 26-27 0.201292631546643 1106 28-29 0.1968196681936547 1106 30-31 0.2126449389101026 1106 32-33 0.25700244382743875 1106 34-35 0.2621572436813935 1106 36-37 0.2619971518804718 1106 38-39 0.27728827535992195 1106 40-41 0.2728182161835946 1106 42-43 0.30305610508214187 1106 44-45 0.28531256744911815 1106 46-47 0.28043943800449966 1106 48-49 0.23342688222564334 1106 50-51 0.23056255536243953 1106 52-53 0.21491352512147444 1106 54-55 0.22459514351245247 1106 56-57 0.2606094242764456 1106 58-59 0.2391262177425304 1106 60-61 0.20895669611761747 1106 62-63 0.18503445151609554 1106 64-65 0.18500039738148644 1106 66-67 0.15620575839671602 1106 68-69 0.19339354743529924 1106 70-71 0.20511413399736256 1106 72-73 0.1246049855591238 1106 74-75 0.15762282541858497 1106 76-77 0.062383426867825165 1106 78-79 0.18326361623291376 1106 80-81 0.24602351652329446 1106 82-83 0.2429937378317888 1106 84-85 0.25094305103748127 1106 86-87 0.27442356623981823 1106 88-89 0.22375239268620106 1106 90-91 0.2261593033141267 1106 92-93 0.24809625667530355 1106 94-95 0.2318177329855402 1106 96-97 0.24261184295466975 1106 98-99 0.25218510538875094 1106 100 0.2627073638680848 1107 1 1.2667782848173452 1107 2 0.895970747311079 1107 3 1.2703963162269005 1107 4 0.7732340280622836 1107 5 0.3808138392859206 1107 6 0.930219918379791 1107 7 0.6465294746162158 1107 8 0.2802132908219619 1107 9 0.273269305169606 1107 10-11 0.1882336134968341 1107 12-13 0.13368357711653545 1107 14-15 0.178183210831385 1107 16-17 0.17880997357674033 1107 18-19 0.14896159609462245 1107 20-21 0.13661454940299933 1107 22-23 0.1633965822566381 1107 24-25 0.17386608013826077 1107 26-27 0.14715004234047768 1107 28-29 0.1389656114796125 1107 30-31 0.1625192827137809 1107 32-33 0.16116688471856122 1107 34-35 0.1847707507404337 1107 36-37 0.15369759479994372 1107 38-39 0.16778447098591442 1107 40-41 0.2025200734159398 1107 42-43 0.22554690458681392 1107 44-45 0.18707810178754158 1107 46-47 0.23181612595906387 1107 48-49 0.20979945373667164 1107 50-51 0.190379323629287 1107 52-53 0.20322908440790144 1107 54-55 0.24493957696449087 1107 56-57 0.2390722599983448 1107 58-59 0.1944015766984606 1107 60-61 0.21042247446948892 1107 62-63 0.18668862763669125 1107 64-65 0.14176919248045294 1107 66-67 0.17053737958929105 1107 68-69 0.16063619411588093 1107 70-71 0.15205268700242414 1107 72-73 0.1694608547871823 1107 74-75 0.17704794603252338 1107 76-77 0.02069253933458981 1107 78-79 0.1580383387872928 1107 80-81 0.2267516319137144 1107 82-83 0.20534362483186186 1107 84-85 0.2342339238253146 1107 86-87 0.24191425678801437 1107 88-89 0.23428476206414928 1107 90-91 0.21587311872658432 1107 92-93 0.1963515421409383 1107 94-95 0.15295561315080164 1107 96-97 0.20367580336786872 1107 98-99 0.24827510738007774 1107 100 0.21630592652092773 1108 1 1.2788027570121194 1108 2 0.9100587057139258 1108 3 1.245759604265821 1108 4 0.7599245841952893 1108 5 0.35581170366469905 1108 6 0.8771648766104647 1108 7 0.607647822346685 1108 8 0.2803259232851474 1108 9 0.22828786757936825 1108 10-11 0.1308923399512949 1108 12-13 0.13805913103157508 1108 14-15 0.14985029962377183 1108 16-17 0.16339492465251482 1108 18-19 0.12727695403286532 1108 20-21 0.14403294492939267 1108 22-23 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-0.7777891266429684 2316 92-93 -0.8521915440821708 2316 94-95 -0.8808344822327854 2316 96-97 -0.9907801521668667 2316 98-99 -1.149907290234939 2316 100 -1.0837567090407525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 50.0 7 849.0 8 1041.0 9 392.0 10 431.0 11 754.0 12 1067.0 13 1684.0 14 4054.0 15 10180.0 16 21355.0 17 33770.0 18 42966.0 19 54672.0 20 70674.0 21 96178.0 22 136600.0 23 216053.0 24 347408.0 25 439468.0 26 447449.0 27 396736.0 28 369693.0 29 396798.0 30 462776.0 31 563777.0 32 696887.0 33 888574.0 34 1215257.0 35 1862961.0 36 3341419.0 37 6729855.0 38 8920448.0 39 2568467.0 40 5335.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.87288740668976 32.16640568165754 26.878728392696015 25.08197851895669 2 20.400436670314374 31.396254187047568 30.62299866854178 17.58031047409628 3 23.503740408371563 26.910935238183875 27.46874618193872 22.116578171505843 4 18.618793331327126 28.7323384288608 26.577903888468622 26.070964351343452 5 21.60494400790244 32.018642415221635 26.994620851703093 19.38179272517283 6 26.933991234459043 27.70979513016421 26.674880214336156 18.681333421040595 7 20.58304302656717 27.27547248258324 31.949651994393314 20.191832496456275 8 24.63772719850748 26.83502282097615 27.871891454503782 20.655358526012584 9 24.41037355799323 25.388289056661623 29.42094197477513 20.780395410570023 10-11 20.35750546607321 29.516612431992183 29.620017970376278 20.505864131558326 12-13 20.41838884149671 27.234154516908397 29.21952646465637 23.12793017693852 14-15 20.3811560096827 32.27509004623266 26.74530296798156 20.598450976103074 16-17 23.050840621234993 27.041192715932993 26.763781167175228 23.144185495656785 18-19 20.441562497796255 29.649992990857005 26.788567207927166 23.11987730341957 20-21 22.991296739341085 29.714384272870646 26.783434565293206 20.510884422495064 22-23 22.963902605108512 27.227334691268734 29.24820441317142 20.560558290451336 24-25 20.531839043023606 29.60665655426618 26.611831271300957 23.249673131409253 26-27 20.60137507382191 27.10472559406761 31.735139202060676 20.55876013004981 28-29 20.581530350485895 27.223661818287276 31.681437218891546 20.513370612335287 30-31 20.56460671556076 27.159250445247473 31.659839533282643 20.616303305909128 32-33 20.70547718346544 27.260628145237764 31.473817800766753 20.560076870530047 34-35 20.69140720631682 27.37896783282516 31.357648690633848 20.57197627022417 36-37 20.63353514307673 27.30493918594057 31.42583857317558 20.635687097807118 38-39 20.677787692461518 27.310936305537066 31.43252786020821 20.57874814179321 40-41 20.71501171040378 27.343727728984472 31.409358719655927 20.531901840955822 42-43 20.724762301626054 27.379626269893677 31.316612655332914 20.578998773147354 44-45 20.764253800284617 27.437880813223238 31.28568624468985 20.512179141802292 46-47 20.780496172962668 27.53893167652151 31.176200659854423 20.504371490661395 48-49 20.820385720766197 27.576801476010115 31.050789860340757 20.552022942882935 50-51 20.854518663023832 27.637583436397396 30.970496557578976 20.537401342999793 52-53 20.950015969159836 27.773694352193207 30.695928599555135 20.580361079091823 54-55 21.23171492498568 27.93386378339178 30.130205573434093 20.704215718188447 56-57 21.58970994201187 28.359412854510314 29.2075030324191 20.843374171058713 58-59 21.674897872784317 28.835402848998715 28.584402835320784 20.90529644289618 60-61 21.76752435965374 29.0907728795053 28.192212675991442 20.94949008484952 62-63 21.75357045426854 29.248334169261238 27.997326437066857 21.000768939403365 64-65 21.762622958256376 29.35115848266972 27.859016366257585 21.027202192816322 66-67 21.717973804390734 29.286521237048564 27.92717684386119 21.068328114699508 68-69 21.66796711103333 29.20091776446129 28.01285089166216 21.118264232843217 70-71 21.643088839117535 29.124930034795067 28.02835538284878 21.20362574323862 72-73 21.619347899336123 29.101492027222932 27.92494377140579 21.35421630203515 74-75 21.687245333729756 29.147919651080763 27.783351184519795 21.38148383066969 76-77 21.71999613492017 29.272728780486695 27.566118971357902 21.441156113235234 78-79 21.803540625287056 29.41910660221293 27.21376124241573 21.563591530084285 80-81 21.851673122067403 29.577503029685985 26.901147016784886 21.669676831461725 82-83 21.851858500627376 29.701491353869113 26.57351171679368 21.873138428709826 84-85 21.840633123420854 29.731153663615927 26.396642763096835 22.031570449866383 86-87 21.83588590423598 29.7282043624882 26.275316479538475 22.16059325373735 88-89 21.837224569093312 29.761131570907253 26.106892777461965 22.294751082537466 90-91 21.883878919184202 29.727078726251214 25.968519101320435 22.420523253244152 92-93 21.881282835742237 29.767161630516703 25.86887800996382 22.48267752377724 94-95 21.927057859153972 29.80133773794819 25.75260888711463 22.518995515783207 96-97 21.97599844426605 29.736627987560066 25.66905447116703 22.61831909700685 98-99 21.97810597652386 29.704469635950815 25.6540131462916 22.663411241233728 100 21.952628961069482 29.65672963895346 25.57370929188828 22.81693210808878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31966.0 1 23283.0 2 11761.5 3 8443.5 4 7805.0 5 7781.0 6 8088.0 7 8231.0 8 8449.5 9 8807.5 10 9681.5 11 17070.5 12 111684.5 13 129323.5 14 57512.0 15 60003.0 16 68024.0 17 76414.5 18 84524.0 19 92902.5 20 104556.5 21 124509.0 22 154149.5 23 187083.0 24 219439.5 25 256678.5 26 299185.5 27 349815.5 28 414839.0 29 477192.5 30 525237.0 31 575360.5 32 632426.0 33 675815.5 34 712907.0 35 762083.5 36 808569.5 37 845960.5 38 890141.5 39 925212.5 40 945093.0 41 981463.0 42 1029494.5 43 1035853.5 44 1011879.0 45 1007669.0 46 1009365.0 47 1004255.5 48 996527.0 49 988777.5 50 981155.0 51 974537.5 52 960054.0 53 920038.0 54 863807.0 55 801579.0 56 732651.5 57 657987.5 58 576059.5 59 486387.5 60 395116.5 61 316297.5 62 246002.5 63 181127.0 64 131270.0 65 93995.5 66 66344.0 67 48553.5 68 35871.0 69 26626.0 70 20020.0 71 15288.5 72 12362.0 73 9790.5 74 7794.5 75 6739.5 76 4977.0 77 2698.0 78 1342.5 79 804.0 80 472.5 81 315.5 82 212.0 83 141.5 84 105.5 85 67.5 86 55.0 87 44.0 88 20.5 89 14.0 90 9.5 91 7.0 92 6.5 93 5.0 94 5.0 95 4.5 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3266649482677794 2 0.006274286911145486 3 2.4172349388939156 4 8.238296889634305E-4 5 3.295318755853722E-5 6 0.008943495103387001 7 0.007625367601045512 8 3.0646464429439615E-4 9 0.0 10-11 8.073530951841619E-5 12-13 3.295318755853722E-6 14-15 0.0 16-17 2.96578688026835E-5 18-19 0.0 20-21 3.130552818061036E-4 22-23 3.295318755853722E-5 24-25 5.519658916054984E-4 26-27 0.0035886021251247033 28-29 0.0063204213737274376 30-31 0.0056580623038008405 32-33 0.015394081567970662 34-35 0.00994691966454446 36-37 0.005185184062335832 38-39 0.005387846165820835 40-41 0.00839317687115943 42-43 0.01924795685294159 44-45 0.034345459732885414 46-47 0.03342936111875808 48-49 0.04000352203668626 50-51 0.04018311690888029 52-53 0.06218925556047143 54-55 0.03362213726597552 56-57 0.022577876455731775 58-59 0.017277356236941065 60-61 0.01825606590742962 62-63 0.024835169803491573 64-65 0.044745485726359766 66-67 0.04792876364451446 68-69 0.05081381521526439 70-71 0.06097328293956141 72-73 0.06841081737152327 74-75 0.059409654189908824 76-77 0.05969305160291224 78-79 0.05443866584670348 80-81 0.05203143549555234 82-83 0.04837033635779885 84-85 0.05003282467012706 86-87 0.05779165268078465 88-89 0.06435592764244526 90-91 0.06079698338612324 92-93 0.06476784248692698 94-95 0.07340816826477543 96-97 0.0729155181107753 98-99 0.04797489810709641 100 0.006903692793513548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0346078E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.65272890705667 #Duplication Level Percentage of deduplicated Percentage of total 1 61.42223400026234 21.898702576771523 2 19.216608945435397 13.702490984890566 3 7.621415677938695 8.151728011606195 4 3.5846631297266764 5.11212091149066 5 1.9811596367472788 3.53168737252768 6 1.26201027237772 2.6996466071402154 7 0.8697357817450222 2.1705917833125596 8 0.6029160304815411 1.719648143078168 9 0.4744123871534214 1.5222686606397493 >10 2.559357472418876 16.79145831305936 >50 0.22343475759913703 5.540091530452036 >100 0.1801954455663374 11.999094844655183 >500 0.0012033562581332843 0.25173073573270144 >1k 5.97432562563115E-4 0.3759589933606586 >5k 1.855790901303939E-5 0.04030380663810512 >10k+ 3.711581801949968E-5 4.492476724644787 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1270130 4.185483211372488 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36707 0.12096126557112256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36517 0.12033515500751035 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00208923209121126 1.3181275023414888E-5 0.0 0.0 1.977191253512233E-5 2 0.002099118047478821 1.647659377926861E-5 0.0 0.0 1.977191253512233E-5 3 0.0021057086849905282 3.295318755853722E-5 0.0 6.590637511707444E-6 2.9657868802683497E-5 4 0.002109004003746382 4.613446258195211E-5 0.0 9.885956267561165E-6 2.9657868802683497E-5 5 0.002109004003746382 4.942978133780583E-5 0.0 9.885956267561165E-6 3.295318755853722E-5 6 0.0021254805975256504 7.249701262878188E-5 0.0 9.885956267561165E-6 4.613446258195211E-5 7 0.0021254805975256504 7.57923313846356E-5 0.0 9.885956267561165E-6 4.613446258195211E-5 8 0.0021254805975256504 7.57923313846356E-5 0.0 9.885956267561165E-6 4.613446258195211E-5 9 0.0021287759162815045 7.57923313846356E-5 0.0 9.885956267561165E-6 4.942978133780583E-5 10-11 0.002145252510060773 8.238296889634305E-5 0.0 1.3181275023414888E-5 4.942978133780583E-5 12-13 0.002171615060107603 9.062126578597736E-5 0.0 1.647659377926861E-5 5.602041884951327E-5 14-15 0.002201272928910286 1.0874551894317282E-4 0.0 1.977191253512233E-5 6.425871573914758E-5 16-17 0.0022457597321143114 1.0874551894317282E-4 0.0 2.3067231290976054E-5 7.57923313846356E-5 18-19 0.0023149614259872393 1.1533615645488027E-4 0.0 2.3067231290976054E-5 8.732594703012363E-5 20-21 0.0023742771635926066 1.30165090856222E-4 0.0 2.4714890668902917E-5 1.0215488143146537E-4 22-23 0.0025044422544488287 1.4169870650171005E-4 0.0 2.6362550046829776E-5 1.0709785956524596E-4 24-25 0.0029839111334255455 1.482893440134175E-4 0.0 2.6362550046829776E-5 1.1698381583280713E-4 26-27 0.004545892223700209 1.5323232214719808E-4 0.0 2.6362550046829776E-5 1.2522211272244144E-4 28-29 0.01011992389922678 1.5487998152512492E-4 0.0 2.6362550046829776E-5 1.2851743147829515E-4 30-31 0.023274836372594837 1.5817530028097864E-4 0.0 2.6362550046829776E-5 1.3675572836792946E-4 32-33 0.05031951740188633 1.6641359717061295E-4 0.0 2.6362550046829776E-5 1.4993700339134433E-4 34-35 0.09859923249389921 1.7794721281610097E-4 0.0 2.9657868802683497E-5 1.5817530028097864E-4 36-37 0.17467660895091613 1.812425315719547E-4 0.0 2.9657868802683497E-5 1.5817530028097864E-4 38-39 0.28681301089386246 1.8783316908366215E-4 0.0 2.9657868802683497E-5 1.647659377926861E-4 40-41 0.4352984263732532 2.0101444410707702E-4 0.0 2.9657868802683497E-5 1.697089159264667E-4 42-43 0.6214130867257377 2.1419571913049192E-4 0.0 3.295318755853722E-5 1.7794721281610097E-4 44-45 0.852472599589311 2.2408167539805308E-4 0.0 3.295318755853722E-5 1.8783316908366215E-4 46-47 1.1375390915425712 2.323199722876874E-4 0.0 3.460084693646408E-5 2.0430976286293077E-4 48-49 1.4787808823268693 2.471489066890291E-4 0.0 3.624850631439094E-5 2.1749103788634564E-4 50-51 1.871945363087777 2.553872035786634E-4 0.0 3.624850631439094E-5 2.2408167539805308E-4 52-53 2.3042153915244006 2.6033018171244405E-4 0.0 3.624850631439094E-5 2.2902465353183367E-4 54-55 2.785648280479606 2.669208192241515E-4 0.0 3.954382507024467E-5 2.356152910435411E-4 56-57 3.326065727505215 2.817497536254932E-4 0.0 4.2839143826098384E-5 2.3891060979939485E-4 58-59 3.9255352866357227 3.0152166616061556E-4 0.0 4.2839143826098384E-5 2.405582691773217E-4 60-61 4.574930572576792 3.163506005619573E-4 0.0 4.2839143826098384E-5 2.405582691773217E-4 62-63 5.260562831216607 3.19645919317811E-4 0.0 4.613446258195211E-5 2.553872035786634E-4 64-65 5.978452965157475 3.2788421620744536E-4 0.0 4.613446258195211E-5 2.702161379800052E-4 66-67 6.738842825092587 3.3117953496329905E-4 0.0 4.613446258195211E-5 2.8010209424756636E-4 68-69 7.544447753676769 3.328271943412259E-4 0.0 4.613446258195211E-5 2.8504507238134695E-4 70-71 8.382068351633446 3.3447485371915274E-4 0.0 4.613446258195211E-5 2.866927317592738E-4 72-73 9.242286597958392 3.657803818997631E-4 0.0 4.9429781337805833E-5 2.866927317592738E-4 74-75 10.123850271524379 3.9214293194659287E-4 3.295318755853722E-6 5.272510009365955E-5 2.8834039113720064E-4 76-77 11.026927433587957 4.086195257258615E-4 3.295318755853722E-6 5.272510009365955E-5 2.9987400678268866E-4 78-79 11.956843319258589 4.135625038596421E-4 3.295318755853722E-6 5.272510009365955E-5 3.08112303672323E-4 80-81 12.909696271129338 4.1850548199342266E-4 3.295318755853722E-6 5.272510009365955E-5 3.229412380736647E-4 82-83 13.87849691811904 4.349820757726913E-4 3.295318755853722E-6 5.272510009365955E-5 3.3447485371915274E-4 84-85 14.857167044782525 4.415727132843987E-4 3.295318755853722E-6 5.272510009365955E-5 3.575420850101288E-4 86-87 15.848993731578755 4.5804930706366733E-4 3.295318755853722E-6 5.602041884951327E-5 3.6248506314390944E-4 88 16.604392831258128 4.712305820870822E-4 3.295318755853722E-6 5.931573760536699E-5 3.8225697567903174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 174655 0.0 82.27626 8 AGTGGTA 175635 0.0 81.19516 5 TGGTATC 177200 0.0 81.05278 7 AAGCAGT 171810 0.0 80.83587 1 GTGGTAT 176315 0.0 80.78083 6 GTATCAA 178550 0.0 80.40507 9 GCAGTGG 177880 0.0 80.36651 3 CAGTGGT 183370 0.0 77.629715 4 AGCAGTG 188140 0.0 75.75098 2 CAACGCA 172330 0.0 43.048237 12-13 ATCAACG 173875 0.0 42.73325 10-11 ACGCAGA 174595 0.0 42.520973 14-15 AGAGTAC 175945 0.0 42.071075 18-19 GAGTACT 168805 0.0 42.01699 20-21 TCAACGC 171685 0.0 41.68711 12-13 AACGCAG 173395 0.0 41.339886 14-15 AGTACTT 177905 0.0 41.283394 20-21 CGCAGAG 175835 0.0 40.749077 16-17 TATCAAC 178780 0.0 40.085274 10-11 GTACTTT 180375 0.0 39.388596 22-23 >>END_MODULE