FastQCFastQC Report
Thu 26 May 2016
SRR1660738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28939891
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15467995.3448680922813425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA563170.19459990364165505No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT405400.1400834578126089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2040050.085.048728
AGTGGTA2059100.084.370365
GTGGTAT2068600.084.0091256
AAGCAGT2084850.083.619671
TGGTATC2073250.083.4396747
GTATCAA2076500.083.428289
GCAGTGG2121600.082.2673343
CAGTGGT2159400.080.317264
AGCAGTG2179300.080.074912
CAACGCA2008400.044.65192812-13
ATCAACG2026350.044.3398610-11
ACGCAGA2042850.043.94253514-15
AGAGTAC2039300.043.3236118-19
TCAACGC2009650.043.2078312-13
AACGCAG2027600.042.94562514-15
GAGTACT1997300.042.41917420-21
CGCAGAG2053050.042.3553716-17
TATCAAC2065850.041.8925810-11
AGTACTT2103900.041.53439720-21
GTACTTT2086000.040.48510422-23
Produced by FastQC (version 0.11.5)