##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660738_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28939891 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83734240740575 34.0 31.0 34.0 30.0 34.0 2 31.955044440215755 34.0 31.0 34.0 30.0 34.0 3 32.016400234541315 34.0 31.0 34.0 30.0 34.0 4 35.418985614009394 37.0 35.0 37.0 33.0 37.0 5 35.38603960187687 37.0 35.0 37.0 33.0 37.0 6 35.458899413270075 37.0 35.0 37.0 33.0 37.0 7 35.41345604929887 37.0 35.0 37.0 33.0 37.0 8 35.42348842986313 37.0 35.0 37.0 33.0 37.0 9 37.104766427765746 39.0 37.0 39.0 33.0 39.0 10-11 37.040342118081924 39.0 37.0 39.0 33.0 39.0 12-13 37.06304465694083 39.0 37.0 39.0 33.0 39.0 14-15 38.43517171505587 40.0 38.0 41.0 33.5 41.0 16-17 38.41018637561558 40.0 38.0 41.0 33.0 41.0 18-19 38.36636786227011 40.0 38.0 41.0 33.5 41.0 20-21 38.24150870160499 40.0 38.0 41.0 33.5 41.0 22-23 38.06155349721255 40.0 38.0 41.0 33.0 41.0 24-25 38.045032287785745 40.0 38.0 41.0 33.0 41.0 26-27 38.02758232572472 40.0 38.0 41.0 33.0 41.0 28-29 37.943690095446456 40.0 38.0 41.0 33.0 41.0 30-31 37.84707739569579 40.0 38.0 41.0 32.0 41.0 32-33 37.71676562292512 40.0 38.0 41.0 32.0 41.0 34-35 37.553454209623666 40.0 37.5 41.0 31.5 41.0 36-37 37.353407049805405 40.0 37.0 41.0 31.0 41.0 38-39 36.92282090834412 39.5 35.0 41.0 30.5 41.0 40-41 36.84976551915831 39.0 35.0 41.0 30.0 41.0 42-43 36.845584853792296 40.0 35.0 41.0 30.0 41.0 44-45 36.83424773438158 40.0 35.0 41.0 30.0 41.0 46-47 36.91555051123033 40.0 35.0 41.0 30.0 41.0 48-49 36.79993373160943 40.0 35.0 41.0 30.0 41.0 50-51 35.42544914906556 38.5 34.0 40.0 28.0 40.5 52-53 35.57835578924606 38.5 34.5 39.5 28.0 40.5 54-55 35.97843632168483 39.0 35.0 41.0 26.5 41.0 56-57 35.63556134333747 39.0 35.0 41.0 24.5 41.0 58-59 35.45398045901417 39.0 35.0 41.0 24.0 41.0 60-61 35.23202970944155 39.0 35.0 41.0 24.0 41.0 62-63 35.00270372476524 38.5 34.5 41.0 23.0 41.0 64-65 34.746161086093935 38.0 34.0 40.5 23.0 41.0 66-67 34.4739078837581 37.0 34.0 40.0 23.0 41.0 68-69 34.18750080986829 37.0 34.0 40.0 22.5 41.0 70-71 33.87398076240163 36.0 34.0 39.0 23.0 41.0 72-73 33.519638584678844 35.5 33.0 39.0 22.0 41.0 74-75 33.12893434878521 35.0 33.0 38.5 20.5 40.0 76-77 32.72680458609882 35.0 33.0 37.0 20.0 39.5 78-79 32.3516819050908 35.0 32.5 37.0 19.5 39.0 80-81 32.027161142383015 35.0 32.0 36.0 19.0 39.0 82-83 31.70753277888987 35.0 32.0 36.0 18.5 37.5 84-85 31.41375845195823 35.0 32.0 35.5 18.0 37.0 86-87 31.19088434023473 35.0 31.5 35.0 17.0 37.0 88-89 30.97650167376235 35.0 31.0 35.0 17.0 36.0 90-91 30.794100364787138 35.0 31.0 35.0 15.0 36.0 92-93 30.632793606582695 35.0 31.0 35.0 15.0 36.0 94-95 30.445331722223834 35.0 31.0 35.0 13.5 35.5 96-97 30.300861672215696 35.0 30.5 35.0 12.0 35.0 98-99 30.19006168682529 34.0 30.0 35.0 10.5 35.0 100 30.104325790307918 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.498986259348559 1101 2 0.5306734825307409 1101 3 0.5211400558640022 1101 4 0.44857160828004794 1101 5 0.45856665981685296 1101 6 0.43902039751881006 1101 7 0.46161873500253137 1101 8 0.47762837757226606 1101 9 0.5590933995035243 1101 10-11 0.5590997302510061 1101 12-13 0.5624623609120718 1101 14-15 0.6834378419519638 1101 16-17 0.640325399583304 1101 18-19 0.6567197295380041 1101 20-21 0.689995484194796 1101 22-23 0.7406657051529635 1101 24-25 0.7251280572851755 1101 26-27 0.6979379961334615 1101 28-29 0.6779707007403744 1101 30-31 0.6431089121959417 1101 32-33 0.6450484525921638 1101 34-35 0.6664897367902824 1101 36-37 0.6387970076354392 1101 38-39 0.634485227035853 1101 40-41 0.6188584971493327 1101 42-43 0.6333979391025721 1101 44-45 0.6654611444950547 1101 46-47 0.6806467379969305 1101 48-49 0.7530268476245894 1101 50-51 0.6838009292171279 1101 52-53 0.7254818080700431 1101 54-55 0.7724450672701408 1101 56-57 0.7802957476018051 1101 58-59 0.6776226666561271 1101 60-61 0.6713159235906829 1101 62-63 0.6557511696939642 1101 64-65 0.6302222519419232 1101 66-67 0.622893329373106 1101 68-69 0.5948199299512424 1101 70-71 0.6114263356145102 1101 72-73 0.5794628079682766 1101 74-75 0.5450290333974124 1101 76-77 0.5608821124070857 1101 78-79 0.5581293727365804 1101 80-81 0.4526761248717932 1101 82-83 0.4923600849182179 1101 84-85 0.4896484328673516 1101 86-87 0.45642581188722886 1101 88-89 0.42302537753401026 1101 90-91 0.39568002830363014 1101 92-93 0.3986023298345174 1101 94-95 0.4178834422045554 1101 96-97 0.4157316741710595 1101 98-99 0.38066727915473564 1101 100 0.3859223967249221 1102 1 0.48368159281369216 1102 2 0.5278513547441968 1102 3 0.5153630143963284 1102 4 0.45209458166606 1102 5 0.4536599859586019 1102 6 0.4008402814113623 1102 7 0.425682301719867 1102 8 0.4407376257252196 1102 9 0.5239261052696307 1102 10-11 0.5075165833434241 1102 12-13 0.5469375252002564 1102 14-15 0.6384357475225926 1102 16-17 0.5981023150847449 1102 18-19 0.5924947980838127 1102 20-21 0.5967384026715905 1102 22-23 0.6192884635489193 1102 24-25 0.5587197688864265 1102 26-27 0.5693988857891199 1102 28-29 0.6090716801794827 1102 30-31 0.5446513042399133 1102 32-33 0.5676657312557865 1102 34-35 0.5766082930135923 1102 36-37 0.5821256404630404 1102 38-39 0.5795836218753152 1102 40-41 0.5758575863346067 1102 42-43 0.5861363970284614 1102 44-45 0.5902601821583886 1102 46-47 0.6208550338481373 1102 48-49 0.6596340567569783 1102 50-51 0.6116178792607556 1102 52-53 0.6469705485614554 1102 54-55 0.7250710736344175 1102 56-57 0.74945692285619 1102 58-59 0.6506100717212107 1102 60-61 0.6520619152173097 1102 62-63 0.6202300566813364 1102 64-65 0.6037731368135795 1102 66-67 0.5956507806183708 1102 68-69 0.546870714245145 1102 70-71 0.5544745907383444 1102 72-73 0.5330289691374119 1102 74-75 0.4939455938955959 1102 76-77 0.4592223316503805 1102 78-79 0.4668320047663954 1102 80-81 0.4432304585210929 1102 82-83 0.4399317598417838 1102 84-85 0.4130690319219248 1102 86-87 0.3794419207638242 1102 88-89 0.39416916929459944 1102 90-91 0.3779840386702773 1102 92-93 0.3721162033814984 1102 94-95 0.4098435380734955 1102 96-97 0.39959922650350777 1102 98-99 0.33951003183991446 1102 100 0.3170662568897349 1103 1 0.4080886039345124 1103 2 0.42173333468331364 1103 3 0.43850609798459317 1103 4 0.35266702860158716 1103 5 0.4096049181835042 1103 6 0.378412978661018 1103 7 0.354603130287785 1103 8 0.3672982884332683 1103 9 0.4321559003946689 1103 10-11 0.4570729416422452 1103 12-13 0.4623892982688673 1103 14-15 0.542139784443485 1103 16-17 0.5107237573858043 1103 18-19 0.5071904651294616 1103 20-21 0.524963081902527 1103 22-23 0.5660776746517229 1103 24-25 0.6011259141519005 1103 26-27 0.5636149847569811 1103 28-29 0.5556693013553087 1103 30-31 0.5267912002030783 1103 32-33 0.4813068917995338 1103 34-35 0.4701813914982935 1103 36-37 0.4991122746006482 1103 38-39 0.5455032670981979 1103 40-41 0.5257094639645459 1103 42-43 0.5294729942540854 1103 44-45 0.5409816894376434 1103 46-47 0.5640631062711279 1103 48-49 0.5802277470996131 1103 50-51 0.5112860747540893 1103 52-53 0.5029944159131077 1103 54-55 0.6000667775229047 1103 56-57 0.6821567705369418 1103 58-59 0.5907602507185956 1103 60-61 0.5763240622261279 1103 62-63 0.5413240199262859 1103 64-65 0.5421417644637145 1103 66-67 0.4837240176286599 1103 68-69 0.48399932788957756 1103 70-71 0.4599585047394825 1103 72-73 0.4354376836184315 1103 74-75 0.46479048447318405 1103 76-77 0.4598371333795228 1103 78-79 0.45903523143169167 1103 80-81 0.3942994834469502 1103 82-83 0.4165230805235751 1103 84-85 0.4213339149455315 1103 86-87 0.3467657413922183 1103 88-89 0.383291085205137 1103 90-91 0.36816728620174644 1103 92-93 0.35560445731321266 1103 94-95 0.3890260862671582 1103 96-97 0.38514583325664375 1103 98-99 0.36212690131121406 1103 100 0.3826721182251376 1104 1 0.3553106800346377 1104 2 0.3899120055998502 1104 3 0.37317600209351554 1104 4 0.317738270687812 1104 5 0.3286884006477422 1104 6 0.2686256156151927 1104 7 0.28736371840904695 1104 8 0.3161090254078829 1104 9 0.36075454902813675 1104 10-11 0.34286483027205605 1104 12-13 0.3522098453152154 1104 14-15 0.4247058600315796 1104 16-17 0.4184221607771548 1104 18-19 0.39306919912427674 1104 20-21 0.34529180128771486 1104 22-23 0.38763740743102204 1104 24-25 0.39301134182723274 1104 26-27 0.34539273555577665 1104 28-29 0.3505892124596315 1104 30-31 0.33850120726923194 1104 32-33 0.3017589210608236 1104 34-35 0.27008230806026745 1104 36-37 0.27957444781272756 1104 38-39 0.2888104710608985 1104 40-41 0.29594368429395246 1104 42-43 0.29028352908585475 1104 44-45 0.3086941116459485 1104 46-47 0.3373033236657861 1104 48-49 0.35433140537231367 1104 50-51 0.31671034625691874 1104 52-53 0.3398651619509465 1104 54-55 0.4735281848090054 1104 56-57 0.4888916641814802 1104 58-59 0.43801689849274794 1104 60-61 0.46847622581213244 1104 62-63 0.4062082822620212 1104 64-65 0.3839471524702347 1104 66-67 0.38788394888067756 1104 68-69 0.3850100023648366 1104 70-71 0.36847460643955543 1104 72-73 0.3324931148629986 1104 74-75 0.3315039991546058 1104 76-77 0.3201779033408485 1104 78-79 0.35552838941541864 1104 80-81 0.33713728168983437 1104 82-83 0.3135875895537481 1104 84-85 0.2808359996631289 1104 86-87 0.2617648457193553 1104 88-89 0.27596749481869765 1104 90-91 0.25324809601938014 1104 92-93 0.2972950678027182 1104 94-95 0.35650255067292846 1104 96-97 0.29552406849664337 1104 98-99 0.3068506097003798 1104 100 0.2608538102358686 1105 1 0.2828712662245998 1105 2 0.32272654103975995 1105 3 0.32710274800216155 1105 4 0.28925205415485067 1105 5 0.29666775257700806 1105 6 0.2647090473451428 1105 7 0.27241063647240793 1105 8 0.2869706964356098 1105 9 0.3069816132961165 1105 10-11 0.31771865078098216 1105 12-13 0.29555096606914333 1105 14-15 0.37688913054334705 1105 16-17 0.33411314992174823 1105 18-19 0.3244755338738443 1105 20-21 0.382089632585803 1105 22-23 0.43675509449483485 1105 24-25 0.44383239487300585 1105 26-27 0.41968682864325046 1105 28-29 0.38645060259341335 1105 30-31 0.3783201810188572 1105 32-33 0.4007506097491884 1105 34-35 0.4388527780754288 1105 36-37 0.3713588099533425 1105 38-39 0.4129038005224146 1105 40-41 0.41235154394873064 1105 42-43 0.42421156250983216 1105 44-45 0.443463033711069 1105 46-47 0.46676235161573487 1105 48-49 0.5138760504066582 1105 50-51 0.36198424195939793 1105 52-53 0.40476273164069454 1105 54-55 0.5143341041583298 1105 56-57 0.5627208763120493 1105 58-59 0.4947343566551652 1105 60-61 0.4752903091503171 1105 62-63 0.45820551526536946 1105 64-65 0.4501002952976165 1105 66-67 0.4581680769997618 1105 68-69 0.4966805764311104 1105 70-71 0.5135549029828894 1105 72-73 0.46556509828005943 1105 74-75 0.42817941705820317 1105 76-77 0.45337910959673877 1105 78-79 0.47131823188281174 1105 80-81 0.40472633257407153 1105 82-83 0.39575047022592 1105 84-85 0.40786153117190693 1105 86-87 0.3391459505306216 1105 88-89 0.338711709716776 1105 90-91 0.3374646991567616 1105 92-93 0.333336073786068 1105 94-95 0.3702722785653023 1105 96-97 0.3681246867894181 1105 98-99 0.3495550148258957 1105 100 0.35732677300421756 1106 1 0.22658093877572938 1106 2 0.2693681288907577 1106 3 0.23325930820423935 1106 4 0.23618027855038548 1106 5 0.24715071972237013 1106 6 0.20686532436832294 1106 7 0.21928264950559395 1106 8 0.22910707383959306 1106 9 0.25047121023219887 1106 10-11 0.22949025480964025 1106 12-13 0.23128920446391987 1106 14-15 0.3062615674590603 1106 16-17 0.2747543750446866 1106 18-19 0.24524322722886183 1106 20-21 0.2910782465758359 1106 22-23 0.2762905954342969 1106 24-25 0.285496129064974 1106 26-27 0.28171394101777736 1106 28-29 0.2694767608647908 1106 30-31 0.2568849600517993 1106 32-33 0.311753600720877 1106 34-35 0.3154677857486021 1106 36-37 0.31364039539256083 1106 38-39 0.3293930102063456 1106 40-41 0.34803664638864973 1106 42-43 0.37114105366953964 1106 44-45 0.3945385310972327 1106 46-47 0.4007998799327339 1106 48-49 0.38977635323661985 1106 50-51 0.2968963268708009 1106 52-53 0.32349083668135137 1106 54-55 0.46196891689115915 1106 56-57 0.46627079925780635 1106 58-59 0.4028300505129536 1106 60-61 0.40315360356874663 1106 62-63 0.37980263154940275 1106 64-65 0.38265315263898714 1106 66-67 0.379150137743558 1106 68-69 0.37382728760013606 1106 70-71 0.3595047869756556 1106 72-73 0.354652271454988 1106 74-75 0.36736767989627594 1106 76-77 0.35374961410653327 1106 78-79 0.383116057021347 1106 80-81 0.3096561173349386 1106 82-83 0.33652266137251274 1106 84-85 0.3416091937340475 1106 86-87 0.28653704938760427 1106 88-89 0.30934178815861557 1106 90-91 0.2931964543407517 1106 92-93 0.2941976722655504 1106 94-95 0.3302179715025346 1106 96-97 0.35356436567612093 1106 98-99 0.3553754918494363 1106 100 0.34384031877247523 1107 1 0.23213693728245133 1107 2 0.2673967089468299 1107 3 0.2552687808283949 1107 4 0.24843924999721168 1107 5 0.26715902664417257 1107 6 0.21384970584960428 1107 7 0.2129361781800938 1107 8 0.21977181595146078 1107 9 0.25675987718592097 1107 10-11 0.22834629486306568 1107 12-13 0.21620353937062475 1107 14-15 0.244614644169161 1107 16-17 0.26342250795531186 1107 18-19 0.2562217069678425 1107 20-21 0.24926537351986155 1107 22-23 0.2536011368285713 1107 24-25 0.3222586845310005 1107 26-27 0.2726919750843777 1107 28-29 0.284646695528437 1107 30-31 0.2547728721199718 1107 32-33 0.21664981060995103 1107 34-35 0.26225184989543493 1107 36-37 0.24613236036416453 1107 38-39 0.2857679437901339 1107 40-41 0.2795401136682969 1107 42-43 0.31032765563328724 1107 44-45 0.290014197612507 1107 46-47 0.32293505091173813 1107 48-49 0.3944600584357758 1107 50-51 0.25299470169959193 1107 52-53 0.26539386315050706 1107 54-55 0.32956761026684234 1107 56-57 0.41970626654846654 1107 58-59 0.314216574450775 1107 60-61 0.3468807362146009 1107 62-63 0.31758772089386156 1107 64-65 0.3011447856844498 1107 66-67 0.31951357479428566 1107 68-69 0.30539022523173287 1107 70-71 0.2870159278022797 1107 72-73 0.2871381106991535 1107 74-75 0.31898285673976545 1107 76-77 0.2818968056010931 1107 78-79 0.2781322395544734 1107 80-81 0.23455467471403324 1107 82-83 0.25345385757076144 1107 84-85 0.260752748100618 1107 86-87 0.2670552708113654 1107 88-89 0.3046656962771763 1107 90-91 0.27966468916340403 1107 92-93 0.2741299040624732 1107 94-95 0.29807202244820985 1107 96-97 0.3045490884565645 1107 98-99 0.3140343239949175 1107 100 0.3221879222388395 1108 1 0.16316355932344706 1108 2 0.20451411726257263 1108 3 0.21866205517585513 1108 4 0.19482690287813398 1108 5 0.22107089177728056 1108 6 0.16848690832608781 1108 7 0.18215588009962147 1108 8 0.21505357055418273 1108 9 0.199766855267292 1108 10-11 0.17948532388972893 1108 12-13 0.16250924169698067 1108 14-15 0.23006879428350402 1108 16-17 0.21071734713279966 1108 18-19 0.19952254396529412 1108 20-21 0.18675907545283366 1108 22-23 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-1.402251027997476 2316 90-91 -1.292606317724868 2316 92-93 -1.1609927436085457 2316 94-95 -1.3443096228364482 2316 96-97 -1.489913632039066 2316 98-99 -1.366345642137908 2316 100 -1.1830057399514935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14913.0 3 3759.0 4 2638.0 5 2146.0 6 3757.0 7 19688.0 8 39389.0 9 46101.0 10 47858.0 11 44983.0 12 43561.0 13 45400.0 14 51820.0 15 62896.0 16 79990.0 17 100158.0 18 109702.0 19 120661.0 20 137945.0 21 166791.0 22 216689.0 23 310753.0 24 442200.0 25 536438.0 26 526463.0 27 451089.0 28 415039.0 29 415656.0 30 463219.0 31 557284.0 32 686539.0 33 882464.0 34 1209965.0 35 1837935.0 36 3154891.0 37 5866937.0 38 7519327.0 39 2299058.0 40 3789.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.883509583322086 33.854323262424174 26.228185477332143 25.033981676921595 2 19.76780632512526 32.60323457465038 30.05651348040066 17.5724456198237 3 24.609766816620628 26.351441789545465 26.872481881557935 22.166309512275973 4 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29.347765763627194 20.331550041374058 24-25 20.272164438992554 29.958044914131087 26.036213595998948 23.733577050877408 26-27 20.2842780707605 26.815888127308657 32.566410490036155 20.333423311894688 28-29 20.28218209916496 26.93856910954639 32.499162745861526 20.280086045427122 30-31 20.244325115630332 26.866952657574846 32.47334286136728 20.415379365427544 32-33 20.367503825646242 27.003214115313718 32.28667288727466 20.342609171765382 34-35 20.371547694485667 27.129041251021434 32.16581253353867 20.333598520954226 36-37 20.322283636330432 27.100046356318003 32.18930215720485 20.388367850146714 38-39 20.38513222079678 27.099360764771745 32.1991407455473 20.316366268884174 40-41 20.39339006104519 27.13184847743645 32.167372144174585 20.307389317343784 42-43 20.398347927148333 27.20709762594744 32.09031569933566 20.30423874756857 44-45 20.398620012944985 27.2933704475017 32.057618221538505 20.250391318014813 46-47 20.39450882951964 27.403594915933333 31.97861926837825 20.223276986168774 48-49 20.50127625625228 27.422875760662833 31.80856875584141 20.267279227243478 50-51 20.533186729635144 27.517261425024124 31.744938480975225 20.204613364365503 52-53 20.651722681030517 27.62962603866163 31.480740708213204 20.237910572094645 54-55 20.99461743928502 27.906449351037804 30.724838053691254 20.374095155985923 56-57 21.43754354068722 28.434817246594303 29.699996611784552 20.427642600933925 58-59 21.571048213301907 28.92502534053062 29.11435420553186 20.38957224063561 60-61 21.702196935862673 29.13095296795812 28.799854554679467 20.366995541499747 62-63 21.768481906585652 29.25563700740926 28.672022612543408 20.30385847346168 64-65 21.924171042230746 29.293484829093675 28.614166170828888 20.168177957846694 66-67 22.081942125404172 29.12999204015771 28.73527630706772 20.052789527370397 68-69 22.21089078225485 28.97042466489132 28.915603915283437 19.903080637570387 70-71 22.376019419259407 28.906611745214217 28.92328829960143 19.794080535924948 72-73 22.57720714140556 28.812318251217476 28.864639795082343 19.745834812294625 74-75 22.82108653275701 28.890170560978014 28.678033939230406 19.61070896703456 76-77 23.123396362048823 29.00586122428082 28.381701204520855 19.4890412091495 78-79 23.42048151484221 29.157812230064362 28.005534508623654 19.416171746469775 80-81 23.694794494988326 29.319721950637618 27.70335209511198 19.282131459262075 82-83 23.947362440236283 29.470643243061307 27.317983672254847 19.264010644447563 84-85 24.136704663189494 29.501335291745086 27.116498042631846 19.245462002433577 86-87 24.388821026150346 29.447139059372272 27.01390149121376 19.15013842326362 88-89 24.64569390917762 29.440283080513385 26.858963704435563 19.055059305873435 90-91 24.86751183020461 29.414886847971864 26.719625426814336 18.99797589500919 92-93 25.063561109432037 29.401148321689806 26.630305430370793 18.904985138507367 94-95 25.23946391141464 29.361885607266625 26.54364870785799 18.855001773460746 96-97 25.419984678997388 29.289645888176057 26.42489069716617 18.86547873566039 98-99 25.628461471514914 29.263822528259997 26.32116878502578 18.786547215199313 100 25.7312742365309 29.29338393086649 26.18830917449887 18.78703265810374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 57050.0 1 39764.0 2 18677.5 3 13938.0 4 12730.0 5 12439.5 6 12779.5 7 13046.0 8 13173.5 9 13491.5 10 14376.0 11 18046.5 12 130833.0 13 160255.5 14 78077.5 15 80945.5 16 90573.5 17 99846.0 18 108003.0 19 116211.0 20 126500.5 21 141054.5 22 160741.5 23 183012.5 24 206737.5 25 237539.0 26 274826.0 27 318264.0 28 369766.0 29 420218.5 30 466934.0 31 515694.5 32 573540.5 33 633541.5 34 679095.0 35 728041.0 36 795022.0 37 852222.5 38 893989.0 39 919765.5 40 928015.5 41 934383.5 42 946513.5 43 953746.5 44 959740.5 45 972061.5 46 973841.0 47 970242.0 48 962750.0 49 946116.5 50 931780.0 51 915214.5 52 884139.5 53 835752.5 54 773553.5 55 710774.0 56 649578.0 57 584437.5 58 516519.0 59 443096.5 60 364785.5 61 294454.5 62 231425.5 63 173677.0 64 127390.0 65 92523.5 66 66749.0 67 49166.0 68 36924.0 69 27542.0 70 21290.5 71 17071.0 72 13953.5 73 11175.0 74 8921.5 75 7421.5 76 5999.0 77 4422.0 78 3306.0 79 2666.0 80 2219.0 81 1849.0 82 1528.5 83 1312.5 84 1152.0 85 970.5 86 854.5 87 760.5 88 640.0 89 539.5 90 470.0 91 406.5 92 364.5 93 333.0 94 274.5 95 224.5 96 184.0 97 153.5 98 130.0 99 95.5 100 53.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4043035269206785 2 0.38483558904903964 3 0.16939939407511934 4 0.17600273615405115 5 0.27514616416488924 6 0.10209091665203576 7 0.13682497974854155 8 0.1422603837726963 9 0.1352700326341934 10-11 0.13190443599113763 12-13 0.13354749677529884 14-15 0.14004544799425817 16-17 0.14415396381416917 18-19 0.1556536615842817 20-21 0.13645697559814585 22-23 0.0990311262748018 24-25 0.09394126605383551 26-27 0.08844539186412277 28-29 0.09819836570911757 30-31 0.10284938530003447 32-33 0.10390329389975933 34-35 0.10425229314097968 36-37 0.10039084114034846 38-39 0.0994509619956758 40-41 0.10053424181867168 42-43 0.06276457641115511 44-45 0.06579154012708617 46-47 0.08436970270551468 48-49 0.08858015394736629 50-51 0.09809124713012914 52-53 0.10335387925268966 54-55 0.1060663981077192 56-57 0.10666073344920339 58-59 0.10597828443790615 60-61 0.10386182864337672 62-63 0.10827269529107764 64-65 0.11550665481082842 66-67 0.12457545192551002 68-69 0.12962557460910962 70-71 0.1279825138249484 72-73 0.1142385090531267 74-75 0.11175232138918562 76-77 0.11389469296895417 78-79 0.11242958724343502 80-81 0.10660544644069322 82-83 0.10944754422191846 84-85 0.11566733267931106 86-87 0.12991064824674012 88-89 0.13768020066143302 90-91 0.14489861071004034 92-93 0.1441505083761373 94-95 0.1339742433722366 96-97 0.13039268185218805 98-99 0.12532700969744495 100 0.11976893762315828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8939891E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.1971683077689 #Duplication Level Percentage of deduplicated Percentage of total 1 64.7929157722784 22.805271615879768 2 17.28915558492773 12.170586380438081 3 6.764999090854046 7.143264348080802 4 3.2552169278670586 4.582976723537411 5 1.7956976604800619 3.1601736392891793 6 1.1225109310034684 2.3705523699503663 7 0.8074498589811602 1.989396401065091 8 0.6288216741834766 1.7706193841447073 9 0.4583457247147297 1.4519224454337585 >10 2.6211583830300444 16.84073410285391 >50 0.27039042617093506 6.7429115552238335 >100 0.18962361321099094 12.063414110108802 >500 0.002999317741036731 0.6451859158847713 >1k 6.466320493975831E-4 0.4847845299817042 >5k 2.931536034861653E-5 0.06141378784801036 >10k+ 3.908714712266259E-5 5.716792690279746 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1546799 5.3448680922813425 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56317 0.19459990364165505 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 40540 0.1400834578126089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017968277765800845 3.455438031884778E-6 0.0 0.0 4.1465256382617336E-5 2 0.0018037386526438542 1.0366314095654334E-5 0.0 0.0 4.1465256382617336E-5 3 0.0018071940906757389 1.0366314095654334E-5 0.0 0.0 4.1465256382617336E-5 4 0.0018071940906757389 1.727719015942389E-5 0.0 0.0 4.1465256382617336E-5 5 0.0018106495287076236 2.7643504255078224E-5 0.0 0.0 4.837613244638689E-5 6 0.0018141049667395085 2.7643504255078224E-5 0.0 0.0 4.837613244638689E-5 7 0.0018175604047713932 4.1465256382617336E-5 0.0 3.455438031884778E-6 4.837613244638689E-5 8 0.0018175604047713932 4.1465256382617336E-5 0.0 6.910876063769556E-6 5.183157047827167E-5 9 0.001821015842803278 4.4920694414502114E-5 0.0 6.910876063769556E-6 5.528700851015645E-5 10-11 0.0018279267188670476 5.355928949421406E-5 6.910876063769556E-6 6.910876063769556E-6 5.528700851015645E-5 12-13 0.0018417484709945867 6.047016555798362E-5 6.910876063769556E-6 1.0366314095654334E-5 5.528700851015645E-5 14-15 0.0018676642562337224 6.565332260581079E-5 6.910876063769556E-6 1.727719015942389E-5 5.8742446542041226E-5 16-17 0.0019350452978554758 6.738104162175317E-5 6.910876063769556E-6 2.0732628191308668E-5 5.8742446542041226E-5 18-19 0.0019661442401424385 6.910876063769556E-5 6.910876063769556E-6 2.4188066223193446E-5 6.565332260581079E-5 20-21 0.002033525281764192 6.910876063769556E-5 6.910876063769556E-6 2.9371223271020613E-5 7.429191768552273E-5 22-23 0.0021700150840236404 6.910876063769556E-5 6.910876063769556E-6 3.455438031884778E-5 7.601963670146512E-5 24-25 0.0025034648541005216 6.910876063769556E-5 6.910876063769556E-6 3.455438031884778E-5 7.601963670146512E-5 26-27 0.0035919278341442267 7.601963670146512E-5 6.910876063769556E-6 3.455438031884778E-5 8.465823178117706E-5 28-29 0.006985167981455079 8.811366981306184E-5 6.910876063769556E-6 4.1465256382617336E-5 9.675226489277378E-5 30-31 0.01581726759095257 9.329682686088901E-5 6.910876063769556E-6 4.4920694414502114E-5 1.0711857898842812E-4 32-33 0.03364905555449397 1.0193542194060095E-4 6.910876063769556E-6 4.4920694414502114E-5 1.105740170203129E-4 34-35 0.06726873988571691 1.105740170203129E-4 6.910876063769556E-6 4.4920694414502114E-5 1.105740170203129E-4 36-37 0.12291684167020532 1.2094033111596724E-4 6.910876063769556E-6 5.355928949421406E-5 1.1402945505219768E-4 38-39 0.2049679454563253 1.3648980225944873E-4 6.910876063769556E-6 5.7014727526098833E-5 1.24395769147852E-4 40-41 0.31635399041413115 1.4858383537104546E-4 6.910876063769556E-6 5.8742446542041226E-5 1.261234881637944E-4 42-43 0.4588959923864261 1.7622733962612368E-4 6.910876063769556E-6 5.8742446542041226E-5 1.2957892619567916E-4 44-45 0.6389467741948303 1.7968277765800846E-4 6.910876063769556E-6 6.738104162175317E-5 1.3130664521162158E-4 46-47 0.8618881805740042 1.848659347058356E-4 6.910876063769556E-6 6.910876063769556E-5 1.3821752127539112E-4 48-49 1.1366179644560512 1.9523224880148998E-4 6.910876063769556E-6 6.910876063769556E-5 1.416729593072759E-4 50-51 1.4553423853600553 2.0732628191308668E-4 6.910876063769556E-6 7.083647965363795E-5 1.451283973391607E-4 52-53 1.8068951952859809 2.1769259600874102E-4 6.910876063769556E-6 7.256419866958034E-5 1.4858383537104546E-4 54-55 2.200708012341857 2.3151434813628013E-4 6.910876063769556E-6 7.94750747333499E-5 1.5203927340293023E-4 56-57 2.649750823180364 2.5570241435947356E-4 6.910876063769556E-6 8.638595079711944E-5 1.572224304507574E-4 58-59 3.1531407633843545 2.643410094391855E-4 6.910876063769556E-6 9.675226489277378E-5 1.606778684826422E-4 60-61 3.7058622646505475 2.695241664870127E-4 6.910876063769556E-6 1.0020770292465857E-4 1.6413330651452696E-4 62-63 4.293070765193967 2.7643504255078224E-4 6.910876063769556E-6 1.0366314095654334E-4 1.6586102553046934E-4 64-65 4.914234127557702 2.833459186145518E-4 6.910876063769556E-6 1.0711857898842812E-4 1.7622733962612368E-4 66-67 5.579964001937672 2.9371223271020615E-4 6.910876063769556E-6 1.1575717406814007E-4 1.7622733962612368E-4 68-69 6.288197491828839 2.9371223271020615E-4 1.3821752127539112E-5 1.2094033111596724E-4 1.7622733962612368E-4 70-71 7.038006812119645 2.971676707420909E-4 1.3821752127539112E-5 1.2094033111596724E-4 1.848659347058356E-4 72-73 7.811945456187102 3.0235082778991805E-4 1.3821752127539112E-5 1.2094033111596724E-4 1.900490917536628E-4 74-75 8.613259808062166 3.092617038536876E-4 1.3821752127539112E-5 1.2094033111596724E-4 1.900490917536628E-4 76-77 9.443458166445755 3.4208836515659305E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.900490917536628E-4 78-79 10.303530514334003 3.489992412203626E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.900490917536628E-4 80-81 11.190342769431993 3.489992412203626E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.9350452978554757E-4 82-83 12.09519241105642 3.576378363000745E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.9350452978554757E-4 84-85 13.020829967880665 3.766427454754408E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.9350452978554757E-4 86-87 13.95790675230947 3.9737537366674945E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.9350452978554757E-4 88 14.670836182485967 4.077416877624038E-4 1.3821752127539112E-5 1.3130664521162158E-4 1.9695996781743234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 204005 0.0 85.04872 8 AGTGGTA 205910 0.0 84.37036 5 GTGGTAT 206860 0.0 84.009125 6 AAGCAGT 208485 0.0 83.61967 1 TGGTATC 207325 0.0 83.439674 7 GTATCAA 207650 0.0 83.42828 9 GCAGTGG 212160 0.0 82.267334 3 CAGTGGT 215940 0.0 80.31726 4 AGCAGTG 217930 0.0 80.07491 2 CAACGCA 200840 0.0 44.651928 12-13 ATCAACG 202635 0.0 44.33986 10-11 ACGCAGA 204285 0.0 43.942535 14-15 AGAGTAC 203930 0.0 43.32361 18-19 TCAACGC 200965 0.0 43.20783 12-13 AACGCAG 202760 0.0 42.945625 14-15 GAGTACT 199730 0.0 42.419174 20-21 CGCAGAG 205305 0.0 42.35537 16-17 TATCAAC 206585 0.0 41.89258 10-11 AGTACTT 210390 0.0 41.534397 20-21 GTACTTT 208600 0.0 40.485104 22-23 >>END_MODULE