FastQCFastQC Report
Thu 26 May 2016
SRR1660738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28939891
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15897385.493241145932443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA499890.17273389177588816No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT434890.15027354456863712No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT351510.12146210225878183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2108500.085.267391
GGTATCA2186550.085.135758
GCAGTGG2194000.084.170123
AGTGGTA2197100.083.993155
GTGGTAT2201250.083.83926
GTATCAA2228550.083.4656759
TGGTATC2230300.083.456477
AGCAGTG2252700.081.709762
CAGTGGT2292100.080.2773364
CAACGCA2152400.044.6681912-13
ATCAACG2172550.044.36952610-11
ACGCAGA2183950.044.10353514-15
AGAGTAC2217500.043.33620518-19
TCAACGC2152000.043.07007612-13
AACGCAG2171950.042.746914-15
GAGTACT2169800.042.29811520-21
CGCAGAG2197850.042.21340616-17
AGTACTT2277950.041.8220320-21
TATCAAC2219200.041.8219510-11
GTACTTT2257150.040.5811722-23
Produced by FastQC (version 0.11.5)