##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660738_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28939891 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.185963692814184 34.0 31.0 34.0 27.0 34.0 2 31.841971623182687 34.0 31.0 34.0 28.0 34.0 3 31.600830355580815 34.0 31.0 34.0 30.0 34.0 4 35.53945642020559 37.0 35.0 37.0 33.0 37.0 5 35.87981706634624 37.0 35.0 37.0 33.0 37.0 6 35.336317679980205 37.0 35.0 37.0 33.0 37.0 7 35.54406600909451 37.0 35.0 37.0 33.0 37.0 8 35.918121979104896 37.0 35.0 37.0 33.0 37.0 9 37.72498151427039 39.0 37.0 39.0 35.0 39.0 10-11 37.737045502348295 39.0 37.5 39.0 35.0 39.0 12-13 37.81719238334381 39.0 38.0 39.0 35.0 39.0 14-15 39.26020374437485 41.0 39.0 41.0 36.0 41.0 16-17 39.22217652789362 41.0 39.0 41.0 36.0 41.0 18-19 39.20888000234693 41.0 39.0 41.0 36.0 41.0 20-21 39.13979003929213 40.5 39.0 41.0 36.0 41.0 22-23 39.004887198780395 40.0 38.5 41.0 35.5 41.0 24-25 39.01819075614348 40.0 39.0 41.0 35.0 41.0 26-27 38.97444169710245 40.0 39.0 41.0 35.0 41.0 28-29 38.89305875409137 40.0 38.0 41.0 35.0 41.0 30-31 38.794394698998694 40.0 38.0 41.0 35.0 41.0 32-33 38.667025801859445 40.0 38.0 41.0 34.5 41.0 34-35 38.57201279714564 40.0 38.0 41.0 34.0 41.0 36-37 38.43103992686082 40.0 38.0 41.0 34.0 41.0 38-39 38.00782262794286 40.0 37.0 41.0 33.5 41.0 40-41 37.8649046743127 40.0 36.5 41.0 33.0 41.0 42-43 37.6889218587589 40.0 36.0 41.0 33.0 41.0 44-45 37.554103987468366 40.0 36.0 41.0 33.0 41.0 46-47 37.388813437479776 40.0 35.0 41.0 33.0 41.0 48-49 37.424201511332576 40.0 35.5 41.0 33.0 41.0 50-51 37.406646521232574 40.0 35.5 41.0 33.0 41.0 52-53 37.141608688159884 40.0 35.0 41.0 31.5 41.0 54-55 36.61378406712036 39.5 35.0 41.0 30.0 41.0 56-57 36.13481825138871 39.0 35.0 41.0 28.0 41.0 58-59 35.99773102117074 39.0 35.0 41.0 28.0 41.0 60-61 35.855284061021514 39.0 35.0 41.0 28.0 41.0 62-63 35.624171787654625 39.0 35.0 41.0 27.0 41.0 64-65 35.358894112628136 38.0 35.0 40.0 27.0 41.0 66-67 35.07903929216596 37.0 35.0 40.0 26.0 41.0 68-69 34.80770843262678 37.0 34.5 40.0 26.0 41.0 70-71 34.49926919213345 36.0 34.0 39.0 26.0 41.0 72-73 34.147960094251914 36.0 34.0 39.0 26.0 41.0 74-75 33.7591198598502 35.0 34.0 38.5 25.5 40.0 76-77 32.04331165241776 34.5 31.5 36.5 23.0 39.0 78-79 32.97589038949732 35.0 33.0 37.0 24.5 39.0 80-81 32.8752134899195 35.0 34.0 36.5 24.5 39.0 82-83 32.61111980345745 35.0 33.5 36.0 24.0 38.0 84-85 32.35529503203726 35.0 33.0 36.0 24.0 37.0 86-87 32.134603893290404 35.0 33.0 35.0 23.0 37.0 88-89 31.952020845551907 35.0 33.0 35.0 23.0 36.0 90-91 31.776530481749223 35.0 33.0 35.0 20.5 36.0 92-93 31.6194342266182 35.0 33.0 35.0 20.0 36.0 94-95 31.499403556841315 35.0 33.0 35.0 20.0 36.0 96-97 31.38271187683464 35.0 33.0 35.0 19.0 35.0 98-99 31.273121432972914 35.0 33.0 35.0 19.0 35.0 100 31.204999148061752 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6049032934833143 1101 2 1.2084252485030689 1101 3 1.5712784637617325 1101 4 0.987660369607184 1101 5 0.6020164666848231 1101 6 1.1249852352886265 1101 7 0.8373902806072593 1101 8 0.49565617720721633 1101 9 0.5312535549712294 1101 10-11 0.4335558883303108 1101 12-13 0.39077995305908075 1101 14-15 0.5243780230129147 1101 16-17 0.5634778431455487 1101 18-19 0.53173037515662 1101 20-21 0.5532402665736598 1101 22-23 0.5331959909542476 1101 24-25 0.5526441974104657 1101 26-27 0.521755874628461 1101 28-29 0.5178878087529597 1101 30-31 0.506480310502603 1101 32-33 0.5545041615774551 1101 34-35 0.5607573250008286 1101 36-37 0.5227939732644202 1101 38-39 0.563080861246597 1101 40-41 0.5782814695023646 1101 42-43 0.5858350686699012 1101 44-45 0.5432342892654489 1101 46-47 0.5704232430342344 1101 48-49 0.5513936131993944 1101 50-51 0.5312511012826633 1101 52-53 0.5214834748589467 1101 54-55 0.5866707168365011 1101 56-57 0.6202380608081199 1101 58-59 0.6041111811923088 1101 60-61 0.566814368986563 1101 62-63 0.5178161697314252 1101 64-65 0.5239463154748805 1101 66-67 0.5075255902154296 1101 68-69 0.4954477027467803 1101 70-71 0.5024659855744034 1101 72-73 0.4679498384165086 1101 74-75 0.4793388102884393 1101 76-77 0.3806592516722276 1101 78-79 0.49443723846667353 1101 80-81 0.49220797693210727 1101 82-83 0.46547977299795207 1101 84-85 0.46514679296981143 1101 86-87 0.4546182993271657 1101 88-89 0.4115937555539837 1101 90-91 0.4111789373245003 1101 92-93 0.3713474369893355 1101 94-95 0.3351700361204344 1101 96-97 0.32858073768019125 1101 98-99 0.27804608057472535 1101 100 0.27672383158945735 1102 1 1.5334713295374947 1102 2 1.165044053813304 1102 3 1.5295317942686104 1102 4 0.932425903116318 1102 5 0.5254383933214015 1102 6 1.0865928860713794 1102 7 0.7989987483635517 1102 8 0.4742781985961102 1102 9 0.4833936767066547 1102 10-11 0.41699035467993895 1102 12-13 0.38802533982758547 1102 14-15 0.49211742890504695 1102 16-17 0.5099644785453847 1102 18-19 0.4634436243391846 1102 20-21 0.4586738914820856 1102 22-23 0.4871982221366835 1102 24-25 0.47617402686613275 1102 26-27 0.4836629981406304 1102 28-29 0.5151009873780907 1102 30-31 0.5108963121285797 1102 32-33 0.49632374907477583 1102 34-35 0.4695871037307384 1102 36-37 0.46965179332340057 1102 38-39 0.49517849128805125 1102 40-41 0.5194036956539065 1102 42-43 0.5177275795025196 1102 44-45 0.5015533221000368 1102 46-47 0.5003102032516793 1102 48-49 0.47411182271297747 1102 50-51 0.437444772947444 1102 52-53 0.4330279493546314 1102 54-55 0.528997125640565 1102 56-57 0.5353419142078408 1102 58-59 0.5285138118692672 1102 60-61 0.5373133989966448 1102 62-63 0.4728265479955809 1102 64-65 0.44177265877566185 1102 66-67 0.44345678378982 1102 68-69 0.41113285532303223 1102 70-71 0.3929622254813694 1102 72-73 0.3594001545652006 1102 74-75 0.42219202336437434 1102 76-77 0.21507906981087643 1102 78-79 0.41382397180780117 1102 80-81 0.44445356614387777 1102 82-83 0.4394513161616942 1102 84-85 0.44937074308646174 1102 86-87 0.43679579130175483 1102 88-89 0.41959296851768 1102 90-91 0.420166719429794 1102 92-93 0.3923259691955643 1102 94-95 0.3453540146156939 1102 96-97 0.35773077080015625 1102 98-99 0.3004392249703258 1102 100 0.2770584930309816 1103 1 1.5183239958336294 1103 2 1.1246632178051144 1103 3 1.4474901563301934 1103 4 0.9124836735873316 1103 5 0.5086551182794565 1103 6 1.045400456334633 1103 7 0.7706503283158668 1103 8 0.4357763639580128 1103 9 0.44457782256721146 1103 10-11 0.3575606498462349 1103 12-13 0.322755445937311 1103 14-15 0.3779605390613412 1103 16-17 0.455749718478927 1103 18-19 0.3878137151514309 1103 20-21 0.41524465382969566 1103 22-23 0.39934950273134007 1103 24-25 0.402091895893399 1103 26-27 0.393830371572065 1103 28-29 0.3874681560918063 1103 30-31 0.38054364209334324 1103 32-33 0.4210448053071474 1103 34-35 0.4424101935976026 1103 36-37 0.3694549033649608 1103 38-39 0.41038219753001215 1103 40-41 0.4290580903211634 1103 42-43 0.4463540347958954 1103 44-45 0.4140179883435309 1103 46-47 0.40383098316532795 1103 48-49 0.4021703397470233 1103 50-51 0.3875034177507928 1103 52-53 0.4357890653479046 1103 54-55 0.46917851707258507 1103 56-57 0.5386047372747882 1103 58-59 0.5302964452475933 1103 60-61 0.5594561179724593 1103 62-63 0.44784060671639025 1103 64-65 0.4383750364063985 1103 66-67 0.47328480129019823 1103 68-69 0.46337911916579344 1103 70-71 0.46073095487572857 1103 72-73 0.4192930103122947 1103 74-75 0.4504627772708645 1103 76-77 0.3272223094421065 1103 78-79 0.4809508358912282 1103 80-81 0.49038077815727377 1103 82-83 0.460857047130915 1103 84-85 0.4473922940977104 1103 86-87 0.44507740098904236 1103 88-89 0.43426771074713955 1103 90-91 0.4413814911232876 1103 92-93 0.4401820232349358 1103 94-95 0.4010293277478034 1103 96-97 0.45036671577489784 1103 98-99 0.42650928967040613 1103 100 0.4123353231885716 1104 1 1.4332261399105484 1104 2 1.048333995025331 1104 3 1.4181208642270668 1104 4 0.8621567339335385 1104 5 0.47036147919230586 1104 6 0.9963896295687817 1104 7 0.7614122352525854 1104 8 0.41990289577868367 1104 9 0.4150120399221535 1104 10-11 0.3114949045040092 1104 12-13 0.27698969561224374 1104 14-15 0.33887498323176857 1104 16-17 0.35135777263636214 1104 18-19 0.3493208479665597 1104 20-21 0.3428028734293136 1104 22-23 0.3589214590746792 1104 24-25 0.33546910263437013 1104 26-27 0.3291581363193714 1104 28-29 0.3209537803160458 1104 30-31 0.34871342581831044 1104 32-33 0.3613267910935747 1104 34-35 0.38311049532941155 1104 36-37 0.37310697252949154 1104 38-39 0.42835579238766996 1104 40-41 0.41169602313774334 1104 42-43 0.45418320088586484 1104 44-45 0.41806009480743 1104 46-47 0.4359465440298038 1104 48-49 0.37397862174533714 1104 50-51 0.3677974235885131 1104 52-53 0.4323239607459044 1104 54-55 0.41049723168828933 1104 56-57 0.4557221469312154 1104 58-59 0.47727627855785215 1104 60-61 0.4509520848953841 1104 62-63 0.3922127227084431 1104 64-65 0.3569108662571949 1104 66-67 0.3946222324787456 1104 68-69 0.3655668294632406 1104 70-71 0.38719254625780763 1104 72-73 0.4010613064555528 1104 74-75 0.3828752342125412 1104 76-77 0.27125202845491714 1104 78-79 0.3874338114181981 1104 80-81 0.42149949839787837 1104 82-83 0.44028057127878384 1104 84-85 0.3913106436531564 1104 86-87 0.37917512094710304 1104 88-89 0.3597538262037041 1104 90-91 0.3818772497150178 1104 92-93 0.3842287825460353 1104 94-95 0.36054550257642504 1104 96-97 0.31218522609165333 1104 98-99 0.31950926616777764 1104 100 0.33577272694230587 1105 1 1.3947590897883266 1105 2 1.0193855668826046 1105 3 1.3675501540027675 1105 4 0.8594286431655931 1105 5 0.4359325124996545 1105 6 1.0057290820894096 1105 7 0.7193462795507841 1105 8 0.3710153802136418 1105 9 0.37126182929668516 1105 10-11 0.28263826328480235 1105 12-13 0.23827456853385343 1105 14-15 0.306159841904865 1105 16-17 0.2937060768878865 1105 18-19 0.27993303291074056 1105 20-21 0.3119789600503893 1105 22-23 0.31101876442805576 1105 24-25 0.2850747229625483 1105 26-27 0.29875917649909667 1105 28-29 0.28798629813677223 1105 30-31 0.2766358369258981 1105 32-33 0.33869064916785874 1105 34-35 0.3481800464113505 1105 36-37 0.3360644547682696 1105 38-39 0.3629825446228807 1105 40-41 0.3720889368625251 1105 42-43 0.3600057922216493 1105 44-45 0.3204788733668025 1105 46-47 0.32052520019392006 1105 48-49 0.32109068497572935 1105 50-51 0.3161262571860135 1105 52-53 0.3086581192348561 1105 54-55 0.3333687611707248 1105 56-57 0.35447580529153555 1105 58-59 0.3318090076528719 1105 60-61 0.2834049157009346 1105 62-63 0.2655032039747667 1105 64-65 0.2457996425679596 1105 66-67 0.25158295737426783 1105 68-69 0.23521979823769357 1105 70-71 0.29067604620927057 1105 72-73 0.25052493564850664 1105 74-75 0.2874814745808649 1105 76-77 0.18644495285818863 1105 78-79 0.3017299376111211 1105 80-81 0.35090871233003895 1105 82-83 0.3920502999158373 1105 84-85 0.364331768761204 1105 86-87 0.3433853173468435 1105 88-89 0.3152815872831276 1105 90-91 0.30053126401122654 1105 92-93 0.26561843259564455 1105 94-95 0.24921613571438073 1105 96-97 0.24386828016206508 1105 98-99 0.2899378274906148 1105 100 0.2330424546042167 1106 1 1.3269636012586155 1106 2 0.9943492046830329 1106 3 1.3543200637667816 1106 4 0.8056201014227824 1106 5 0.4249387127067621 1106 6 0.9448569659629058 1106 7 0.6859535867644126 1106 8 0.32507816970324654 1106 9 0.33521661409632486 1106 10-11 0.22866777106366243 1106 12-13 0.20367208156557837 1106 14-15 0.24211566846229715 1106 16-17 0.26049457485866867 1106 18-19 0.21749580498558174 1106 20-21 0.23931262633762884 1106 22-23 0.26237870747973346 1106 24-25 0.24865871706233378 1106 26-27 0.24696445428383385 1106 28-29 0.24346549156440034 1106 30-31 0.25480857580911476 1106 32-33 0.2821997946373642 1106 34-35 0.29917429696980946 1106 36-37 0.299405448844972 1106 38-39 0.3320547880215443 1106 40-41 0.3305079550786232 1106 42-43 0.34254667078919 1106 44-45 0.31202590658466534 1106 46-47 0.3265040448038832 1106 48-49 0.29584130456910174 1106 50-51 0.330021571236486 1106 52-53 0.26731377509905485 1106 54-55 0.3438913720643626 1106 56-57 0.4113868179831215 1106 58-59 0.40721237680818234 1106 60-61 0.3876891919626999 1106 62-63 0.32790312919933484 1106 64-65 0.2716411729265502 1106 66-67 0.27637184888559574 1106 68-69 0.28850668169092586 1106 70-71 0.2828015266508359 1106 72-73 0.2330836241935188 1106 74-75 0.2735657605653117 1106 76-77 0.1619420645093257 1106 78-79 0.2940691235052597 1106 80-81 0.2911494959051879 1106 82-83 0.30118558728612044 1106 84-85 0.31648715859518717 1106 86-87 0.33213494459402426 1106 88-89 0.3330804786191628 1106 90-91 0.3361451933838424 1106 92-93 0.3169388100334807 1106 94-95 0.3089230299765191 1106 96-97 0.28064796613966436 1106 98-99 0.30211894224192193 1106 100 0.3307017800674785 1107 1 1.2922594141787762 1107 2 0.9053143142508588 1107 3 1.2844710302937905 1107 4 0.7865802097241925 1107 5 0.37676338580047997 1107 6 0.940803064761937 1107 7 0.6614961996798456 1107 8 0.288272416671596 1107 9 0.25621389945571593 1107 10-11 0.17682606929424338 1107 12-13 0.13536341456529044 1107 14-15 0.16087229213186305 1107 16-17 0.17196053067488037 1107 18-19 0.128966574305295 1107 20-21 0.13693344338570768 1107 22-23 0.1966672098309914 1107 24-25 0.18199732650345624 1107 26-27 0.152911187122875 1107 28-29 0.1369429342987516 1107 30-31 0.13989383846691794 1107 32-33 0.19403715518296138 1107 34-35 0.20123879108344767 1107 36-37 0.15797355612490804 1107 38-39 0.2146412709115495 1107 40-41 0.23260538782736973 1107 42-43 0.23812303386257128 1107 44-45 0.21803338269334205 1107 46-47 0.27976859039055313 1107 48-49 0.2317367793406433 1107 50-51 0.20597171736955033 1107 52-53 0.20712043008813907 1107 54-55 0.2593328221891298 1107 56-57 0.2656522729651769 1107 58-59 0.26724548536721926 1107 60-61 0.28607909016043465 1107 62-63 0.2380395602761496 1107 64-65 0.1980228547882632 1107 66-67 0.23550280953057978 1107 68-69 0.24713048894663814 1107 70-71 0.23510781314385554 1107 72-73 0.20910505976470262 1107 74-75 0.2342542705601076 1107 76-77 0.05472082962980096 1107 78-79 0.24345131244778884 1107 80-81 0.2812026146699509 1107 82-83 0.27845328184743323 1107 84-85 0.28325824684276313 1107 86-87 0.2588247269622812 1107 88-89 0.2769745388495757 1107 90-91 0.309076637876629 1107 92-93 0.2857837563521848 1107 94-95 0.25152892168665986 1107 96-97 0.25804213308654056 1107 98-99 0.29873783700286083 1107 100 0.3155343096031764 1108 1 1.278887119798494 1108 2 0.9067151342540924 1108 3 1.2640325940369088 1108 4 0.7446694021297233 1108 5 0.3291054886026643 1108 6 0.8746086633325305 1108 7 0.6262090736457608 1108 8 0.26279612379187256 1108 9 0.2077802741712773 1108 10-11 0.1450398686713683 1108 12-13 0.13705777636296546 1108 14-15 0.13521305222291602 1108 16-17 0.15674620189081168 1108 18-19 0.133701605143429 1108 20-21 0.1436096949132164 1108 22-23 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-0.9121553243207323 2316 94-95 -0.8927535427542246 2316 96-97 -1.0024486175025125 2316 98-99 -1.1393823820709343 2316 100 -1.053798545794752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 63.0 7 813.0 8 986.0 9 405.0 10 396.0 11 721.0 12 988.0 13 1633.0 14 4054.0 15 10533.0 16 21731.0 17 35834.0 18 45579.0 19 55410.0 20 72318.0 21 100726.0 22 150667.0 23 242500.0 24 398329.0 25 528873.0 26 528156.0 27 437520.0 28 385933.0 29 402476.0 30 460933.0 31 557498.0 32 681128.0 33 859154.0 34 1166149.0 35 1783013.0 36 3162547.0 37 6234059.0 38 8231105.0 39 2373719.0 40 3942.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 15.63156193831888 33.62675464302258 26.594769634428815 24.146913784229724 2 20.020285357919317 32.921081173431 29.74286783167694 17.31576563697275 3 24.951699309492266 26.470039263193023 26.933417759339555 21.644843667975156 4 18.376885076391318 28.28042373383862 25.983131255512326 27.35955993425773 5 21.15465127143617 33.32623240351582 26.430586838808413 19.088529486239608 6 28.179948119547866 27.148324432248266 26.233467010877586 18.438260437326278 7 20.213351736111353 26.779781088035453 33.11970629276866 19.887160883084533 8 26.200627391882747 26.389046063783354 27.084161273086583 20.32616527124732 9 25.871845889122387 25.111887947331933 28.575767614328612 20.440498549217065 10-11 19.94067248732341 30.015489189538798 29.879391440764152 20.164446882373642 12-13 19.990862438405514 26.791061761920492 29.49759962780781 23.720476171866185 14-15 19.96577492292559 33.63309315850568 26.11881122841824 20.28232069015049 16-17 23.565768772651158 26.608749022175644 26.1244744731281 23.701007732045102 18-19 20.077909070217302 30.12041406790371 26.132404921635676 23.669271940243313 20-21 23.512845019487266 30.164697253486217 26.140411871935587 20.18204585509093 22-23 23.45425931275458 26.771225381722385 29.557536174994503 20.216979130528532 24-25 20.125558820615648 30.031161645077496 26.00845660585168 23.83482292845517 26-27 20.179087609311953 26.666070697411538 32.92759046116997 20.22725123210654 28-29 20.172031717085446 26.775709554333467 32.87279908621487 20.17945964236622 30-31 20.145788412595035 26.71653712728447 32.832544979109734 20.30512948101076 32-33 20.311877764146296 26.82592759592543 32.61720709505644 20.24498754487183 34-35 20.277811025200002 26.934462168704272 32.56265261452343 20.225074191572297 36-37 20.219883983044355 26.921621645670808 32.57380904310935 20.284685328175488 38-39 20.27422903342623 26.92853540743343 32.57318558644924 20.2240499726911 40-41 20.28804194149433 26.96019976188298 32.555370933222264 20.196387363400422 42-43 20.29473604028958 27.014675525324495 32.46680098577017 20.223787448615756 44-45 20.30986877943867 27.104011618683614 32.41380893240572 20.172310669471994 46-47 20.342453224499863 27.223767621480526 32.29921857749497 20.134560576524642 48-49 20.392103946431465 27.278271054080573 32.15257442249529 20.17705057699267 50-51 20.436899969849634 27.37079987104686 32.03353231008293 20.15876784902057 52-53 20.543352529206558 27.505173826927344 31.730615933917466 20.220857709948632 54-55 20.848711028503356 27.7812329217734 30.975139226460346 20.394916823262896 56-57 21.289248827781464 28.36278677255388 29.83329025465178 20.514674145012883 58-59 21.3685203205143 28.928303636191966 29.144769883500242 20.558406159793492 60-61 21.357588417225138 29.207599943182387 28.823847775801298 20.61096386379118 62-63 21.336218163024746 29.363707998455247 28.66367901225223 20.63639482626778 64-65 21.36312087156641 29.446762653310337 28.564933135867815 20.625183339255443 66-67 21.34303145270796 29.3247049953886 28.677557178523326 20.654706373380108 68-69 21.283365991512017 29.20970457321328 28.840782796474222 20.666146638800477 70-71 21.237706222826382 29.1894010985827 28.873693761324287 20.699198917266628 72-73 21.22005566474817 29.12611967763526 28.814402205442 20.839422452174567 74-75 21.302382457785264 29.191641646883244 28.619353970100867 20.886621925230628 76-77 21.33104240575747 29.336612063517748 28.389301755458696 20.94304377526609 78-79 21.435487012090135 29.524563115962994 28.017506235150258 21.022443636796613 80-81 21.48006061675807 29.73042061238054 27.668108055091228 21.121410715770157 82-83 21.533098962613725 29.878329131045806 27.264972247132913 21.323599659207552 84-85 21.5273620904306 29.911214454810104 27.070536119436955 21.49088733532234 86-87 21.601293923619977 29.900592572928765 26.902704131089823 21.595409372361438 88-89 21.61429396178205 29.948651049289538 26.733349512856098 21.703705476072315 90-91 21.69324748060795 29.90300683345623 26.5796038129829 21.824141872952918 92-93 21.711968995195928 29.869716139500525 26.521579994851873 21.896734870451674 94-95 21.728252077333202 29.826481783066576 26.461201305392674 21.98406483420755 96-97 21.70672775767357 29.798228786355672 26.382327131802192 22.112716324168563 98-99 21.693987141311112 29.792080013829676 26.35286913512437 22.161063709734837 100 21.672482520042863 29.763136917844328 26.27533199849595 22.289048563616856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45333.0 1 32549.5 2 15496.5 3 10358.0 4 9288.5 5 9163.5 6 9409.5 7 9805.5 8 10179.0 9 10686.5 10 12102.5 11 21897.0 12 145684.5 13 170079.5 14 77810.0 15 81471.5 16 92545.0 17 102661.0 18 111571.5 19 120825.0 20 131674.5 21 147760.0 22 168981.0 23 191504.5 24 215506.0 25 246030.5 26 283894.5 27 328216.5 28 379605.0 29 430900.5 30 478110.0 31 527572.0 32 585607.5 33 645091.0 34 689799.5 35 735237.5 36 797474.5 37 850610.0 38 889773.5 39 913558.0 40 919919.5 41 926825.5 42 939715.5 43 948705.5 44 956794.5 45 970927.0 46 974080.5 47 969163.0 48 960149.5 49 946339.5 50 934744.0 51 919269.5 52 890175.0 53 839870.5 54 775938.0 55 713610.0 56 650062.0 57 581887.0 58 511582.5 59 436098.0 60 356823.0 61 285774.5 62 223115.5 63 165066.5 64 118972.0 65 85463.5 66 60736.5 67 43341.5 68 31304.0 69 22707.0 70 16954.0 71 13005.5 72 10365.0 73 7905.5 74 5859.0 75 4537.5 76 3369.5 77 2108.5 78 1183.0 79 729.5 80 485.5 81 336.5 82 229.0 83 149.0 84 111.0 85 73.5 86 43.0 87 35.5 88 28.0 89 21.0 90 17.0 91 10.5 92 6.5 93 5.5 94 3.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3267634974851841 2 0.00614031338265925 3 2.4371377210784932 4 7.429191768552273E-4 5 6.565332260581079E-5 6 0.008966861692741 7 0.00770908224913494 8 2.902567946783214E-4 9 0.0 10-11 1.0193542194060095E-4 12-13 1.3821752127539112E-5 14-15 0.0 16-17 1.900490917536628E-5 18-19 0.0 20-21 3.2653889401311156E-4 22-23 3.800981835073256E-5 24-25 5.114048287189471E-4 26-27 0.0035867446770963996 28-29 0.006209422143296946 30-31 0.005706655909657711 32-33 0.015273036100930717 34-35 0.009711508588612168 36-37 0.005005201989185101 38-39 0.005271270717640229 40-41 0.00842608564075103 42-43 0.01921396317629531 44-45 0.03433150456579121 46-47 0.03308581915529675 48-49 0.03970471070537204 50-51 0.03992067558236484 52-53 0.06176941025797229 54-55 0.03316011107298227 56-57 0.022113075685046637 58-59 0.01691436916607599 60-61 0.017971733203832732 62-63 0.02474266402731095 64-65 0.044867135125007895 66-67 0.04745353049187366 68-69 0.050166049346903205 70-71 0.06059110588909958 72-73 0.06839175724607947 74-75 0.05912945560161233 76-77 0.059117361568500726 78-79 0.05379425928038222 80-81 0.05143937826165275 82-83 0.047617663798388186 84-85 0.04977731256831616 86-87 0.057705815132475795 88-89 0.06413292987178147 90-91 0.05991038459681828 92-93 0.06460114172510187 94-95 0.07285100002622678 96-97 0.07204588296479762 98-99 0.047310129813550435 100 0.007028360956853639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8939891E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.44465383264889 #Duplication Level Percentage of deduplicated Percentage of total 1 62.7522152043956 21.61478329947293 2 18.618795392619315 12.82635924159381 3 7.0358650177804964 7.270438048520797 4 3.3379743269782916 4.599014807801456 5 1.86920343781926 3.2192032679240827 6 1.1991838328585902 2.4783283202713937 7 0.8932403102865565 2.1537147294031826 8 0.6261763057224734 1.725474087105401 9 0.4754367885075202 1.4738630039503051 >10 2.7162867669164643 17.101905406616147 >50 0.28433868476141017 6.924888269739263 >100 0.18806898082496074 11.511240183786992 >500 0.002489741539318705 0.5179860096756557 >1k 6.246737529174976E-4 0.44728450713064916 >5k 5.0267618478047956E-5 0.11425496993671218 >10k+ 5.026761846233191E-5 6.021261847071364 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1589738 5.493241145932443 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49989 0.17273389177588816 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 43489 0.15027354456863712 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 35151 0.12146210225878183 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0018244712808351629 0.0 0.0 3.455438031884778E-6 1.3821752127539112E-5 2 0.001834837594930817 3.455438031884778E-6 0.0 3.455438031884778E-6 1.3821752127539112E-5 3 0.0018417484709945867 6.910876063769556E-6 0.0 6.910876063769556E-6 2.0732628191308668E-5 4 0.0018417484709945867 1.3821752127539112E-5 0.0 2.4188066223193446E-5 2.4188066223193446E-5 5 0.0018417484709945867 1.3821752127539112E-5 0.0 2.4188066223193446E-5 2.4188066223193446E-5 6 0.001852114785090241 2.0732628191308668E-5 0.0 2.4188066223193446E-5 2.7643504255078224E-5 7 0.001852114785090241 2.4188066223193446E-5 0.0 2.4188066223193446E-5 2.7643504255078224E-5 8 0.001852114785090241 3.455438031884778E-5 0.0 2.4188066223193446E-5 2.7643504255078224E-5 9 0.0018590256611540105 6.2197884573926E-5 0.0 2.4188066223193446E-5 2.7643504255078224E-5 10-11 0.0018624810991858954 6.39256035898684E-5 0.0 2.4188066223193446E-5 2.7643504255078224E-5 12-13 0.0018676642562337224 7.601963670146512E-5 0.0 2.4188066223193446E-5 3.1098942286963E-5 14-15 0.001900490917536628 7.94750747333499E-5 0.0 2.7643504255078224E-5 4.1465256382617336E-5 16-17 0.001976510554238093 8.293051276523467E-5 0.0 3.1098942286963E-5 5.528700851015645E-5 18-19 0.002007609496525056 8.638595079711944E-5 0.0 3.1098942286963E-5 5.8742446542041226E-5 20-21 0.002088812290274348 9.675226489277378E-5 0.0 3.1098942286963E-5 5.8742446542041226E-5 22-23 0.0022304852495816244 1.0539085997248574E-4 0.0 3.1098942286963E-5 6.565332260581079E-5 24-25 0.0025622073006425633 1.1402945505219768E-4 0.0 3.1098942286963E-5 6.910876063769556E-5 26-27 0.003642031685606556 1.24395769147852E-4 0.0 3.1098942286963E-5 6.910876063769556E-5 28-29 0.007088831122411622 1.3130664521162158E-4 0.0 3.1098942286963E-5 6.910876063769556E-5 30-31 0.015986584054514925 1.4512839733916066E-4 0.0 3.455438031884778E-5 6.910876063769556E-5 32-33 0.03408444074651145 1.5203927340293023E-4 0.0 3.800981835073256E-5 7.429191768552273E-5 34-35 0.06846604916376499 1.589501494666998E-4 0.0 4.319297539855972E-5 8.120279374929229E-5 36-37 0.12485361468707673 1.6586102553046934E-4 0.0 4.837613244638689E-5 8.638595079711946E-5 38-39 0.20801391408143174 1.744996206101813E-4 0.0 5.183157047827167E-5 8.638595079711946E-5 40-41 0.32063873357366823 1.7968277765800846E-4 0.0 5.183157047827167E-5 9.156910784494663E-5 42-43 0.4643417627246765 1.883213727377204E-4 0.0 5.183157047827167E-5 9.847998390871617E-5 44-45 0.6459233035812055 1.900490917536628E-4 0.0 5.183157047827167E-5 1.0193542194060095E-4 46-47 0.8705181370586365 1.900490917536628E-4 0.0 5.183157047827167E-5 1.0366314095654334E-4 48-49 1.1470119220559607 1.900490917536628E-4 0.0 5.183157047827167E-5 1.0711857898842812E-4 50-51 1.4675763637119434 1.9695996781743234E-4 0.0 5.183157047827167E-5 1.105740170203129E-4 52-53 1.8222304292714855 2.038708438812019E-4 0.0 5.183157047827167E-5 1.1230173603625528E-4 54-55 2.2195176892684216 2.055985628971443E-4 0.0 5.7014727526098833E-5 1.1402945505219768E-4 56-57 2.673232252326037 2.1078171994497148E-4 0.0 6.910876063769556E-5 1.1402945505219768E-4 58-59 3.1822113635466005 2.194203150246834E-4 0.0 6.910876063769556E-5 1.1402945505219768E-4 60-61 3.7411215543278997 2.3324206715222252E-4 0.0 7.256419866958034E-5 1.1402945505219768E-4 62-63 4.333819018184968 2.5397469534353114E-4 0.0 7.256419866958034E-5 1.1402945505219768E-4 64-65 4.960654482078043 2.79890480582667E-4 0.0 7.256419866958034E-5 1.1402945505219768E-4 66-67 5.63362349913481 2.816181995986094E-4 0.0 7.601963670146512E-5 1.1921261210002485E-4 68-69 6.350267525195585 2.833459186145518E-4 0.0 7.601963670146512E-5 1.24395769147852E-4 70-71 7.108321520630469 2.919845136942638E-4 0.0 7.601963670146512E-5 1.3476208324350635E-4 72-73 7.891525921780424 3.058062658218029E-4 0.0 8.293051276523467E-5 1.3476208324350635E-4 74-75 8.70314438986657 3.127171418855724E-4 0.0 8.811366981306184E-5 1.416729593072759E-4 76-77 9.544388055919079 3.213557369652844E-4 0.0 9.329682686088901E-5 1.416729593072759E-4 78-79 10.415343305888747 3.230834559812268E-4 0.0 9.847998390871617E-5 1.451283973391607E-4 80-81 11.31241993966045 3.265388940131115E-4 0.0 1.0020770292465857E-4 1.5203927340293023E-4 82-83 12.227948612522418 3.3690520810876587E-4 0.0 1.0366314095654335E-4 1.5203927340293023E-4 84-85 13.164724773842444 3.610932743319593E-4 0.0 1.0711857898842812E-4 1.589501494666998E-4 86-87 14.113565597050798 3.887367785870375E-4 0.0 1.0884629800437051E-4 1.606778684826422E-4 88 14.835370319812192 3.939199356348647E-4 0.0 1.105740170203129E-4 1.6586102553046934E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 210850 0.0 85.26739 1 GGTATCA 218655 0.0 85.13575 8 GCAGTGG 219400 0.0 84.17012 3 AGTGGTA 219710 0.0 83.99315 5 GTGGTAT 220125 0.0 83.8392 6 GTATCAA 222855 0.0 83.465675 9 TGGTATC 223030 0.0 83.45647 7 AGCAGTG 225270 0.0 81.70976 2 CAGTGGT 229210 0.0 80.277336 4 CAACGCA 215240 0.0 44.66819 12-13 ATCAACG 217255 0.0 44.369526 10-11 ACGCAGA 218395 0.0 44.103535 14-15 AGAGTAC 221750 0.0 43.336205 18-19 TCAACGC 215200 0.0 43.070076 12-13 AACGCAG 217195 0.0 42.7469 14-15 GAGTACT 216980 0.0 42.298115 20-21 CGCAGAG 219785 0.0 42.213406 16-17 AGTACTT 227795 0.0 41.82203 20-21 TATCAAC 221920 0.0 41.82195 10-11 GTACTTT 225715 0.0 40.58117 22-23 >>END_MODULE