FastQCFastQC Report
Thu 26 May 2016
SRR1660737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28151509
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13653444.84998512868351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377700.13416687538845606No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT364130.1293465298787358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1760300.084.31818
AGTGGTA1779500.083.5788965
GTGGTAT1789600.083.0648966
TGGTATC1783650.083.0031747
GTATCAA1785250.082.991199
AAGCAGT1829100.081.8013841
GCAGTGG1869050.080.111653
CAGTGGT1874450.079.277674
AGCAGTG1931600.077.535412
CAACGCA1735600.044.30582412-13
ATCAACG1743350.044.1972810-11
ACGCAGA1765250.043.5647714-15
AGAGTAC1752150.043.26516318-19
TCAACGC1728600.043.02229312-13
GAGTACT1706600.042.6383220-21
AACGCAG1750100.042.60286714-15
AGTACTT1791150.041.94030420-21
CGCAGAG1787850.041.58966416-17
TATCAAC1785100.041.49697510-11
GTACTTT1800650.040.41250222-23
Produced by FastQC (version 0.11.5)