##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660737_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28151509 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84706830458005 34.0 31.0 34.0 30.0 34.0 2 31.965500321847756 34.0 31.0 34.0 30.0 34.0 3 32.027270722858944 34.0 31.0 34.0 30.0 34.0 4 35.436366270809856 37.0 35.0 37.0 33.0 37.0 5 35.407435565887425 37.0 35.0 37.0 33.0 37.0 6 35.47568888758325 37.0 35.0 37.0 33.0 37.0 7 35.4372037037162 37.0 35.0 37.0 33.0 37.0 8 35.43962158476123 37.0 35.0 37.0 33.0 37.0 9 37.11826140474388 39.0 37.0 39.0 33.0 39.0 10-11 37.06215917235555 39.0 37.0 39.0 33.0 39.0 12-13 37.07340949289788 39.0 37.0 39.0 33.0 39.0 14-15 38.43737046209495 40.0 38.0 41.0 33.0 41.0 16-17 38.414764462537335 40.0 38.0 41.0 33.0 41.0 18-19 38.37580731462743 40.0 38.0 41.0 33.5 41.0 20-21 38.257408990047395 40.0 38.0 41.0 33.5 41.0 22-23 38.09161089730571 40.0 38.0 41.0 33.0 41.0 24-25 38.06616135568434 40.0 38.0 41.0 33.0 41.0 26-27 38.03466382565851 40.0 38.0 41.0 33.0 41.0 28-29 37.94415848187747 40.0 38.0 41.0 33.0 41.0 30-31 37.84729791926962 40.0 38.0 41.0 32.0 41.0 32-33 37.71365584700983 40.0 38.0 41.0 32.0 41.0 34-35 37.551217432074424 40.0 37.5 41.0 31.5 41.0 36-37 37.35965864209979 40.0 37.0 41.0 31.0 41.0 38-39 36.963625058251765 39.5 35.0 41.0 30.5 41.0 40-41 36.8956887000267 39.0 35.0 41.0 30.0 41.0 42-43 36.903222097259516 40.0 35.0 41.0 30.0 41.0 44-45 36.90386700052207 40.0 35.0 41.0 30.0 41.0 46-47 36.998433245621044 40.0 35.0 41.0 31.0 41.0 48-49 36.888664760386376 40.0 35.0 41.0 30.0 41.0 50-51 35.504834199118775 38.5 34.0 40.0 28.0 40.5 52-53 35.67651865482593 38.5 34.5 39.5 28.0 40.5 54-55 36.112774949293126 39.0 35.0 41.0 27.5 41.0 56-57 35.78554726853186 39.0 35.0 41.0 26.0 41.0 58-59 35.59568623834694 39.0 35.0 41.0 26.0 41.0 60-61 35.35313915143945 39.0 35.0 41.0 25.5 41.0 62-63 35.102983982137516 38.0 35.0 41.0 25.0 41.0 64-65 34.832292631276005 37.5 34.0 40.0 25.0 41.0 66-67 34.54895776634922 37.0 34.0 40.0 24.5 41.0 68-69 34.2616460098107 36.5 34.0 39.5 24.0 41.0 70-71 33.95195507992129 36.0 34.0 39.0 24.0 41.0 72-73 33.61006775871233 35.0 33.0 39.0 24.0 41.0 74-75 33.234406457572135 35.0 33.0 37.5 23.5 40.0 76-77 32.84890342823186 35.0 33.0 37.0 22.5 39.5 78-79 32.488269580859765 35.0 33.0 37.0 21.5 39.0 80-81 32.177510413384944 35.0 32.0 36.0 20.0 39.0 82-83 31.879641087801012 35.0 32.0 36.0 20.0 37.0 84-85 31.60497240130183 35.0 32.0 35.0 20.0 37.0 86-87 31.396034312050553 35.0 32.0 35.0 19.0 36.5 88-89 31.192713594855608 35.0 31.5 35.0 19.0 36.0 90-91 31.02197054516687 35.0 31.0 35.0 18.5 36.0 92-93 30.872658336006072 35.0 31.0 35.0 18.0 36.0 94-95 30.693155400657208 35.0 31.0 35.0 17.5 35.0 96-97 30.554991847861512 34.5 31.0 35.0 17.0 35.0 98-99 30.445804184067008 34.0 30.5 35.0 17.0 35.0 100 30.35754797371608 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49556601001313183 1101 2 0.5146416613123144 1101 3 0.49929521047534564 1101 4 0.4237092441053889 1101 5 0.43949237489416504 1101 6 0.4178481219087544 1101 7 0.44924342104371817 1101 8 0.4724631642735915 1101 9 0.5611944313900636 1101 10-11 0.5664645364401721 1101 12-13 0.5610008809875708 1101 14-15 0.6507861744397729 1101 16-17 0.6257886701132165 1101 18-19 0.6361310998574581 1101 20-21 0.671829812207335 1101 22-23 0.7025169624639389 1101 24-25 0.6962884543889842 1101 26-27 0.6841045970502506 1101 28-29 0.675689163489352 1101 30-31 0.6290367518190649 1101 32-33 0.601229832402062 1101 34-35 0.6439000967726614 1101 36-37 0.5719539287900659 1101 38-39 0.6049230718164083 1101 40-41 0.5968880899363356 1101 42-43 0.5938605513373005 1101 44-45 0.6211238431000865 1101 46-47 0.662825935820166 1101 48-49 0.6894608071459842 1101 50-51 0.6201316510978003 1101 52-53 0.6674463604919438 1101 54-55 0.705131428925263 1101 56-57 0.7526615943091386 1101 58-59 0.6300945170027035 1101 60-61 0.62376919547782 1101 62-63 0.5926430593324383 1101 64-65 0.5836696949231168 1101 66-67 0.5602663423230752 1101 68-69 0.5436071766198154 1101 70-71 0.4877318840140248 1101 72-73 0.4881968390195084 1101 74-75 0.4868066677877252 1101 76-77 0.4656119966017158 1101 78-79 0.49389744917173317 1101 80-81 0.4573989246892509 1101 82-83 0.46168050818094386 1101 84-85 0.4234036862920476 1101 86-87 0.384079289294057 1101 88-89 0.35548843592112433 1101 90-91 0.31275291575810016 1101 92-93 0.3186491816433623 1101 94-95 0.3379459709153423 1101 96-97 0.32542908960392936 1101 98-99 0.3105671239755097 1101 100 0.2567411276372731 1102 1 0.454150430475007 1102 2 0.5093352899740431 1102 3 0.49682619546651097 1102 4 0.4449694493871803 1102 5 0.4330573380013618 1102 6 0.38391509958145775 1102 7 0.4073901447372208 1102 8 0.45995500806191103 1102 9 0.5384208196438891 1102 10-11 0.4962299165089945 1102 12-13 0.4992566893051986 1102 14-15 0.5933352978934394 1102 16-17 0.5845488478326715 1102 18-19 0.535156195783685 1102 20-21 0.566053359907599 1102 22-23 0.6034200421937967 1102 24-25 0.5653176206174706 1102 26-27 0.5265927050741581 1102 28-29 0.5475663673133582 1102 30-31 0.5581979844565765 1102 32-33 0.5466605893535004 1102 34-35 0.5176392569025623 1102 36-37 0.5343443278545053 1102 38-39 0.5668077881682194 1102 40-41 0.5470003318603816 1102 42-43 0.5668928617804738 1102 44-45 0.5833380516614923 1102 46-47 0.601825374210037 1102 48-49 0.6481656767339032 1102 50-51 0.583971428461382 1102 52-53 0.6124714997875103 1102 54-55 0.6925160681507663 1102 56-57 0.7248162915863006 1102 58-59 0.6258274532679877 1102 60-61 0.5968219339177523 1102 62-63 0.5815965733599384 1102 64-65 0.5740778060056115 1102 66-67 0.5702634475199488 1102 68-69 0.5433719966908939 1102 70-71 0.5253942237547236 1102 72-73 0.488121846212934 1102 74-75 0.4687057158990626 1102 76-77 0.4617689251256749 1102 78-79 0.44750406802804577 1102 80-81 0.4592932469282047 1102 82-83 0.45161684683593606 1102 84-85 0.417158569390331 1102 86-87 0.3755923218409123 1102 88-89 0.3541677134332204 1102 90-91 0.36065111597622135 1102 92-93 0.3497372189037229 1102 94-95 0.3692156138162197 1102 96-97 0.3516975563166156 1102 98-99 0.3091961734389166 1102 100 0.30469722167847024 1103 1 0.3998783073442489 1103 2 0.4204532827416294 1103 3 0.45732930110246883 1103 4 0.38142848890743863 1103 5 0.3640945232702393 1103 6 0.3602674710173588 1103 7 0.38439414319958587 1103 8 0.4085485851821531 1103 9 0.4641534853489233 1103 10-11 0.4434100667179237 1103 12-13 0.4485782135979903 1103 14-15 0.557793513248491 1103 16-17 0.5224502309913177 1103 18-19 0.5202502933023823 1103 20-21 0.5293269259753401 1103 22-23 0.5610636408129963 1103 24-25 0.6135868287779331 1103 26-27 0.5685274533634157 1103 28-29 0.5784589670225628 1103 30-31 0.4895864457382544 1103 32-33 0.4912662271607928 1103 34-35 0.5058827503439076 1103 36-37 0.5250485013464541 1103 38-39 0.5232704451017582 1103 40-41 0.49843574395144685 1103 42-43 0.5243100561538938 1103 44-45 0.5517679166160079 1103 46-47 0.5655704695918757 1103 48-49 0.5877829834797197 1103 50-51 0.5357707852733213 1103 52-53 0.49527198897026636 1103 54-55 0.5924830416306648 1103 56-57 0.6658426761882126 1103 58-59 0.5741556706669684 1103 60-61 0.5947321711883546 1103 62-63 0.5943934887482598 1103 64-65 0.5621459356619525 1103 66-67 0.4992589951137276 1103 68-69 0.5099041918101861 1103 70-71 0.5046899145311983 1103 72-73 0.489064972193745 1103 74-75 0.4746910293833224 1103 76-77 0.4777016889371879 1103 78-79 0.46581393369688584 1103 80-81 0.407217925884531 1103 82-83 0.4051813876621786 1103 84-85 0.3815128788883939 1103 86-87 0.36286198612618037 1103 88-89 0.4122931091138575 1103 90-91 0.37506751240704617 1103 92-93 0.31465230956600365 1103 94-95 0.37264955212921436 1103 96-97 0.3676755073250604 1103 98-99 0.32745896603732305 1103 100 0.3458509906210452 1104 1 0.34939226929708056 1104 2 0.3980019189655444 1104 3 0.36855581490216593 1104 4 0.31479145954637033 1104 5 0.3337504791407184 1104 6 0.28043113086067706 1104 7 0.29349935471680766 1104 8 0.31226515836199553 1104 9 0.3835498364853791 1104 10-11 0.34277809639396395 1104 12-13 0.34866432551022797 1104 14-15 0.4427806247756365 1104 16-17 0.4330371870463594 1104 18-19 0.39636254651107805 1104 20-21 0.3513166910712613 1104 22-23 0.40487676995826405 1104 24-25 0.4117341204635707 1104 26-27 0.3765484708809623 1104 28-29 0.34892904987348317 1104 30-31 0.35333665773083567 1104 32-33 0.2821223715963086 1104 34-35 0.27856122962484875 1104 36-37 0.2944099351342331 1104 38-39 0.33833816790261295 1104 40-41 0.30610261643474956 1104 42-43 0.31852854844163403 1104 44-45 0.38132651117262384 1104 46-47 0.36791568530414764 1104 48-49 0.4144601360970199 1104 50-51 0.35653588041902395 1104 52-53 0.3679301516162141 1104 54-55 0.4666800114341072 1104 56-57 0.508698249659453 1104 58-59 0.4347869866906038 1104 60-61 0.4672167251003003 1104 62-63 0.4036468523820176 1104 64-65 0.4077894545859664 1104 66-67 0.39112580475327974 1104 68-69 0.36531614057786044 1104 70-71 0.3518031973784872 1104 72-73 0.3713497654016322 1104 74-75 0.34432930441438003 1104 76-77 0.35724228751802656 1104 78-79 0.3418611764506707 1104 80-81 0.29135899895205597 1104 82-83 0.29520484889908616 1104 84-85 0.31501368847487754 1104 86-87 0.2830246981456277 1104 88-89 0.25672796242585605 1104 90-91 0.24496985901853563 1104 92-93 0.26518250473537464 1104 94-95 0.30075597908265195 1104 96-97 0.25527551874241183 1104 98-99 0.2425737552363394 1104 100 0.2084575444379908 1105 1 0.2693098633389859 1105 2 0.31642906351587285 1105 3 0.31021973871056474 1105 4 0.2443894365497954 1105 5 0.25640573487949325 1105 6 0.21977513387285086 1105 7 0.2773283809942626 1105 8 0.2678637754833204 1105 9 0.2754732464113374 1105 10-11 0.29120049356262 1105 12-13 0.29657707980460657 1105 14-15 0.3639182158810499 1105 16-17 0.31236069494796226 1105 18-19 0.31131603095877125 1105 20-21 0.34787967735373115 1105 22-23 0.37974497103642335 1105 24-25 0.39769862895895614 1105 26-27 0.38411072864116136 1105 28-29 0.37093066637834227 1105 30-31 0.39417648837689967 1105 32-33 0.3754944182198159 1105 34-35 0.3919195037872356 1105 36-37 0.36638132100382137 1105 38-39 0.40419818493827364 1105 40-41 0.3940960397902913 1105 42-43 0.45512499918872606 1105 44-45 0.45997080620158926 1105 46-47 0.4502113968899266 1105 48-49 0.47935670922374385 1105 50-51 0.36232516973220186 1105 52-53 0.4015442070169186 1105 54-55 0.5064734208744497 1105 56-57 0.526654653933555 1105 58-59 0.45995544338106953 1105 60-61 0.4856212130379731 1105 62-63 0.456897012508243 1105 64-65 0.45126102900072596 1105 66-67 0.4170228691015012 1105 68-69 0.43745899635469954 1105 70-71 0.4038267466181864 1105 72-73 0.375103764516318 1105 74-75 0.35144369764369543 1105 76-77 0.3883126449574874 1105 78-79 0.4204290966223496 1105 80-81 0.37161401032316377 1105 82-83 0.3539423192618152 1105 84-85 0.3536416791209973 1105 86-87 0.2706737963127246 1105 88-89 0.29879398561421766 1105 90-91 0.3060363569905995 1105 92-93 0.27500544062666776 1105 94-95 0.3172646419727698 1105 96-97 0.34293287508146975 1105 98-99 0.307123119729912 1105 100 0.33079742440882853 1106 1 0.21903013955751405 1106 2 0.27312960574693435 1106 3 0.24202378448743644 1106 4 0.23429460566318028 1106 5 0.22570763713921593 1106 6 0.1941854484848733 1106 7 0.22899089685180485 1106 8 0.2413035604306799 1106 9 0.2638589243606049 1106 10-11 0.2318958762701797 1106 12-13 0.24696890149542838 1106 14-15 0.3356460123706242 1106 16-17 0.2984731498555959 1106 18-19 0.27646464508614343 1106 20-21 0.30018353931205866 1106 22-23 0.2920538230877341 1106 24-25 0.27243340643020986 1106 26-27 0.29314094743169505 1106 28-29 0.3127809206918144 1106 30-31 0.2737959053705694 1106 32-33 0.28577348476640196 1106 34-35 0.3266670416345363 1106 36-37 0.2607881357038977 1106 38-39 0.26728647825511587 1106 40-41 0.3022597991174436 1106 42-43 0.3105622741247629 1106 44-45 0.32134839017452776 1106 46-47 0.3211841562183224 1106 48-49 0.31545413734709626 1106 50-51 0.20978079537548666 1106 52-53 0.2672097225487349 1106 54-55 0.3771685831151146 1106 56-57 0.3793539949404945 1106 58-59 0.26651062273057136 1106 60-61 0.2441683374842043 1106 62-63 0.27399456306336845 1106 64-65 0.2365171622903972 1106 66-67 0.24714857913254917 1106 68-69 0.2385197967448036 1106 70-71 0.2410129703682884 1106 72-73 0.231497314607779 1106 74-75 0.2343389520062047 1106 76-77 0.20938617282451588 1106 78-79 0.23624655592980304 1106 80-81 0.21981060073854763 1106 82-83 0.27011099493661916 1106 84-85 0.2590462776374274 1106 86-87 0.21574882758452318 1106 88-89 0.23475443586145062 1106 90-91 0.18067053843002512 1106 92-93 0.1561894462277209 1106 94-95 0.2142732357381405 1106 96-97 0.25110995428349625 1106 98-99 0.22851431593136695 1106 100 0.19919709794691798 1107 1 0.18828918525917615 1107 2 0.22790958991336296 1107 3 0.23288145291926554 1107 4 0.2227288037645465 1107 5 0.22656243851290725 1107 6 0.19221554684132514 1107 7 0.19818684716300083 1107 8 0.2277680190778213 1107 9 0.23495828867276458 1107 10-11 0.186464858362136 1107 12-13 0.20683004642301483 1107 14-15 0.2517990822095797 1107 16-17 0.24501896037282478 1107 18-19 0.2280225617639573 1107 20-21 0.23861966459689654 1107 22-23 0.23650615118101115 1107 24-25 0.2915842772164652 1107 26-27 0.2628601523211245 1107 28-29 0.27291793780984364 1107 30-31 0.25786158638825185 1107 32-33 0.20038286342852274 1107 34-35 0.24997460575392694 1107 36-37 0.22737292458287328 1107 38-39 0.233588434526375 1107 40-41 0.25423302277265947 1107 42-43 0.3148775344719894 1107 44-45 0.27158293427912383 1107 46-47 0.2831902088145455 1107 48-49 0.3353991251232884 1107 50-51 0.22782356248986702 1107 52-53 0.2533535778132574 1107 54-55 0.3432984515215267 1107 56-57 0.3934002923126485 1107 58-59 0.3406029422720209 1107 60-61 0.36781684814379645 1107 62-63 0.35083396992033755 1107 64-65 0.31412197051722046 1107 66-67 0.35806495513472925 1107 68-69 0.3294502608611367 1107 70-71 0.33755579460574836 1107 72-73 0.31897150922561934 1107 74-75 0.2915268626524252 1107 76-77 0.2807577121590583 1107 78-79 0.2610279688843846 1107 80-81 0.2216137486746632 1107 82-83 0.29134558933986554 1107 84-85 0.258844046262638 1107 86-87 0.25132798428197134 1107 88-89 0.24014889711893872 1107 90-91 0.2844213681968526 1107 92-93 0.2642724227457869 1107 94-95 0.3024210762339301 1107 96-97 0.3306814172483321 1107 98-99 0.2985743717940643 1107 100 0.29207928041199693 1108 1 0.13520021532179882 1108 2 0.1681443965632461 1108 3 0.19869467451080425 1108 4 0.18450078224091015 1108 5 0.19190793874756906 1108 6 0.12099276820926974 1108 7 0.1524209833586383 1108 8 0.16541369168360376 1108 9 0.19148162359433485 1108 10-11 0.137737656569918 1108 12-13 0.12716750246844555 1108 14-15 0.20534831172117407 1108 16-17 0.20077258643032536 1108 18-19 0.1660803911010973 1108 20-21 0.15965002209350132 1108 22-23 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2316 86-87 -1.3937183757670368 2316 88-89 -1.3506507362189524 2316 90-91 -1.2280876007619241 2316 92-93 -1.0477305285796064 2316 94-95 -1.2810704017010757 2316 96-97 -1.4682997569433915 2316 98-99 -1.3245489781682735 2316 100 -1.1475147584137808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14715.0 3 3645.0 4 2483.0 5 2116.0 6 3592.0 7 17918.0 8 35136.0 9 42475.0 10 44090.0 11 40988.0 12 39472.0 13 41173.0 14 46968.0 15 56730.0 16 70784.0 17 87248.0 18 96788.0 19 109038.0 20 124816.0 21 151586.0 22 193523.0 23 277635.0 24 396841.0 25 479182.0 26 476875.0 27 421125.0 28 394599.0 29 398665.0 30 451595.0 31 547943.0 32 683831.0 33 886472.0 34 1226640.0 35 1878715.0 36 3281769.0 37 6001852.0 38 7085644.0 39 2032160.0 40 4682.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.68529139204433 32.45730239951415 25.983970854464882 25.87343535397664 2 20.74084766272866 31.602025589516806 29.372517114544067 18.28460963321046 3 24.67069826187094 25.69797152093445 26.660357247214385 22.97097296998023 4 18.84400915568667 27.945084668466468 25.363760054509388 27.847146121337474 5 22.099425295851795 31.830606090343018 26.236522217981513 19.833446395823675 6 27.469705495654306 27.04547842621657 26.1181199868078 19.366696091321327 7 21.18638967295 26.303555152355745 31.733082705697118 20.776972468997137 8 25.946558797662767 26.123138525282698 26.732450461967698 21.197852215086836 9 25.597115385128415 24.62257612370806 28.366261715768896 21.41404677539462 10-11 20.959322876104803 29.0509370618874 28.94439839220067 21.04534166980713 12-13 20.961521798423856 26.43155375866073 28.63649068546121 23.9704337574542 14-15 20.92892466526934 32.08605907786426 25.900613324200805 21.0844029326656 16-17 23.86545071405742 26.248712705592027 25.959030443086473 23.92680613726408 18-19 21.00466801234194 29.095944550318443 25.94659763141774 23.95278980592188 20-21 23.83537377176145 29.20828987533344 25.91216624740735 21.044170105497763 22-23 23.835524573593307 26.414144453581713 28.705476454638124 21.04485451818686 24-25 21.093690569044014 29.06238070475177 25.77760301709411 24.06632570911011 26-27 21.108292525588325 26.2859279436427 31.554362394250155 21.05141713651882 28-29 21.09102188492786 26.401635590205053 31.499645467685305 21.00769705718178 30-31 21.075786992841252 26.31016304432568 31.465342566504134 21.148707396328938 32-33 21.165019595766964 26.45584289534102 31.317795993157706 21.06134151573431 34-35 21.203600924030876 26.567379408665186 31.190700349804374 21.038319317499564 36-37 21.12884473569892 26.537639151193055 31.241448976974333 21.09206713613369 38-39 21.193150733543167 26.50706732715669 31.275501485206497 21.024280454093645 40-41 21.235263275264213 26.5493194545239 31.220575865031496 20.994841405180384 42-43 21.232011614281397 26.575408534957027 31.18609362976471 21.006486220996866 44-45 21.265134707355372 26.677338830900197 31.152860331014224 20.904666130730202 46-47 21.264398270636995 26.792363258169733 31.07256389408552 20.870674577107753 48-49 21.378106954940705 26.773268699149227 30.93119843414843 20.91742591176164 50-51 21.40296540777175 26.830705021002405 30.898276246638645 20.86805332458721 52-53 21.554193245631506 26.957976779522525 30.620187165837205 20.867642809008768 54-55 21.911964816123845 27.16677906827703 29.918706885836265 21.00254922976286 56-57 22.38739210070047 27.666866366178024 28.917352761467306 21.028388771654203 58-59 22.562206284451282 28.118718795334374 28.31921061663783 20.99986430357651 60-61 22.76032874207407 28.29891300878878 27.965222525425094 20.97553572371206 62-63 22.849884435316216 28.45042473228432 27.806734662166726 20.892956170232733 64-65 22.98513985609859 28.502631354899364 27.749531330695064 20.762697458306977 66-67 23.122215483798207 28.346483633658725 27.880300598244133 20.651000284298938 68-69 23.271509594205952 28.210361527597993 28.062557126576166 20.45557175161989 70-71 23.44258878565653 28.116571902817693 28.09846451211955 20.34237479940623 72-73 23.635307808496904 28.031717272148022 28.040538630240285 20.292436289114793 74-75 23.868756645649523 28.097772396061156 27.898482924903718 20.13498803338561 76-77 24.199865971602396 28.210680350593364 27.567055629253158 20.022398048551082 78-79 24.517030463887597 28.277135537233463 27.24439296942018 19.96144102945876 80-81 24.788232298699423 28.41858223173969 26.96397189611605 19.829213573444832 82-83 25.026227269240657 28.531289636284026 26.64273777663097 19.799745317844348 84-85 25.186210518134335 28.523352160430722 26.50285550267216 19.787581818762796 86-87 25.40146364190679 28.48638074486882 26.424695527505538 19.687460085718847 88-89 25.650633192718697 28.469224375202046 26.27063083933962 19.60951159273964 90-91 25.83967175307253 28.416276306667477 26.17592375743219 19.568128182827802 92-93 25.961189298961667 28.437980057497334 26.12276537592682 19.478065267614177 94-95 26.11129778217876 28.423474413680584 26.01509277227984 19.450135031860817 96-97 26.227395814834075 28.38504042931586 25.930622267482573 19.45694148836749 98-99 26.39018025379348 28.391912633002285 25.87839962703414 19.339507486170096 100 26.48332168773632 28.401126925342247 25.784767246144035 19.330784140777396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 37796.0 1 26201.0 2 12649.0 3 9952.5 4 9192.5 5 9172.0 6 9336.0 7 9447.0 8 9489.5 9 9771.5 10 10382.5 11 13319.0 12 112786.0 13 137313.0 14 62809.5 15 64031.5 16 71350.0 17 79100.5 18 86411.0 19 94036.0 20 103842.5 21 118298.5 22 138240.0 23 160128.5 24 182116.5 25 209661.0 26 241540.5 27 277562.0 28 321135.5 29 366180.5 30 407179.5 31 449214.0 32 495872.0 33 538116.5 34 574250.0 35 616687.0 36 664285.5 37 706803.0 38 748448.0 39 782432.5 40 803248.5 41 828201.5 42 861899.0 43 880196.5 44 891479.5 45 914838.0 46 938634.0 47 959006.0 48 971817.5 49 976271.5 50 975367.5 51 969894.0 52 956103.5 53 924993.0 54 880559.0 55 830904.0 56 771534.0 57 700592.0 58 622293.5 59 535905.0 60 444371.5 61 360137.5 62 285209.0 63 217967.5 64 164523.0 65 123115.0 66 90894.0 67 68294.0 68 51912.0 69 39787.0 70 31277.0 71 25268.5 72 20590.0 73 16163.0 74 12633.5 75 10344.0 76 8275.0 77 5872.5 78 4107.0 79 3190.5 80 2569.0 81 2103.0 82 1761.5 83 1459.0 84 1241.0 85 1033.5 86 884.0 87 801.5 88 696.0 89 609.0 90 540.5 91 475.0 92 388.5 93 351.0 94 317.0 95 259.5 96 225.0 97 178.5 98 130.0 99 98.5 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4030263528679759 2 0.38195110606681865 3 0.16871919725510984 4 0.17474374109039767 5 0.2737473149307911 6 0.10242434961479331 7 0.13718980392845015 8 0.1425429805556782 9 0.1357795775707796 10-11 0.13251154671673196 12-13 0.13436224679820893 14-15 0.14045961088622283 16-17 0.1446085891878833 18-19 0.1560680104217504 20-21 0.137248415351376 22-23 0.09937833172637389 24-25 0.09483861060520771 26-27 0.08918882465590033 28-29 0.09888812709826673 30-31 0.10324313343203022 32-33 0.10430701956332074 34-35 0.10480788081377805 36-37 0.10092531807087143 38-39 0.09998398309660772 40-41 0.10103721260554807 42-43 0.06347794713242547 44-45 0.06685609641742472 46-47 0.08540394761787015 48-49 0.08957246306050592 50-51 0.09882951567534087 52-53 0.103639204562711 54-55 0.10613107808892233 56-57 0.10689125048323342 58-59 0.10625718145339917 60-61 0.10422354268824453 62-63 0.10897284404896376 64-65 0.11557106938743496 66-67 0.12424733608418646 68-69 0.12898420471883051 70-71 0.12758641108723515 72-73 0.11458000350887051 74-75 0.11228172528868702 76-77 0.11400809810941219 78-79 0.11268667693799292 80-81 0.10702445826261035 82-83 0.1100047603132038 84-85 0.11626197373647004 86-87 0.12991133086329404 88-89 0.13761606882245636 90-91 0.14421429416092757 92-93 0.14312909478493677 94-95 0.1335221497362717 96-97 0.13014577655499746 98-99 0.12526149131117625 100 0.1193044394174394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8151509E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.6333889162196 #Duplication Level Percentage of deduplicated Percentage of total 1 60.03591249268536 23.79426668762726 2 19.667731654354487 15.589977155137491 3 8.680945954598458 10.321659215378519 4 4.035299073659168 6.397303103183781 5 2.128729354166028 4.218437919551759 6 1.2468052472406241 2.96490703600026 7 0.8452657383284436 2.3450522021308706 8 0.5705468181117206 1.8090163149706746 9 0.42635262316262623 1.5208019396329309 >10 2.089368259094824 14.759851535809648 >50 0.17556556543851126 4.899976457081699 >100 0.09617344898112248 5.615337792236261 >500 7.294844048869736E-4 0.18283535718296723 >1k 5.206034759398099E-4 0.4036129759187629 >5k 1.7894099302011823E-5 0.036711934548123645 >10k+ 3.578819853214751E-5 5.140252373608945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1365344 4.84998512868351 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37770 0.13416687538845606 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36413 0.1293465298787358 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003292896306198009 1.0656622350155368E-5 0.0 7.104414900103579E-6 3.907428195056968E-5 2 0.003310657343448268 1.0656622350155368E-5 0.0 1.4208829800207158E-5 4.262648940062147E-5 3 0.0033213139657984232 2.4865452150362527E-5 0.0 1.4208829800207158E-5 4.262648940062147E-5 4 0.0033355227955986304 2.8417659600414316E-5 0.0 1.4208829800207158E-5 4.262648940062147E-5 5 0.003342627210498734 3.1969867050466104E-5 0.0 1.4208829800207158E-5 4.262648940062147E-5 6 0.003349731625398837 3.907428195056968E-5 0.0 1.7761037250258947E-5 4.262648940062147E-5 7 0.003356836040298941 3.907428195056968E-5 0.0 1.7761037250258947E-5 4.262648940062147E-5 8 0.0033710448700991482 3.907428195056968E-5 0.0 1.7761037250258947E-5 4.262648940062147E-5 9 0.0033745970775492 3.907428195056968E-5 0.0 1.7761037250258947E-5 4.262648940062147E-5 10-11 0.0033976864259745365 3.907428195056968E-5 0.0 1.7761037250258947E-5 5.328311175077684E-5 12-13 0.0034314323967500285 3.907428195056968E-5 0.0 4.262648940062147E-5 6.216363037590632E-5 14-15 0.0034811633010507536 4.262648940062147E-5 0.0 4.617869685067326E-5 8.880518625129473E-5 16-17 0.0035397747239766086 4.617869685067326E-5 0.0 5.328311175077684E-5 9.235739370134652E-5 18-19 0.0036214754953277995 5.328311175077684E-5 0.0 5.683531920082863E-5 9.235739370134652E-5 20-21 0.003728041718829353 7.992466762616525E-5 0.0 6.038752665088042E-5 1.0479011977652778E-4 22-23 0.003921637024857176 8.525297880124294E-5 0.0 6.038752665088042E-5 1.1367063840165726E-4 24-25 0.004516631772740851 1.0656622350155369E-4 0.0 6.92680452760099E-5 1.1722284585170905E-4 26-27 0.0068078055780242544 1.1722284585170907E-4 0.0 7.459635645108757E-5 1.2255115702678674E-4 28-29 0.01347174675432141 1.2610336447683854E-4 3.5522074500517895E-6 8.170077135119115E-5 1.2432726075181264E-4 30-31 0.028790641382669752 1.332077793769421E-4 3.5522074500517895E-6 9.413349742637242E-5 1.314316756519162E-4 32-33 0.05927035740783913 1.34983883101968E-4 3.5522074500517895E-6 1.030140160515019E-4 1.34983883101968E-4 34-35 0.11341843167270359 1.367599868269939E-4 3.5522074500517895E-6 1.030140160515019E-4 1.5274492035222695E-4 36-37 0.20054519990384884 1.385360905520198E-4 3.5522074500517895E-6 1.030140160515019E-4 1.5274492035222695E-4 38-39 0.32725066354347115 1.4741660917714925E-4 3.5522074500517895E-6 1.0479011977652778E-4 1.5274492035222695E-4 40-41 0.49108202334730977 1.5274492035222695E-4 3.5522074500517895E-6 1.0656622350155369E-4 1.5629712780227873E-4 42-43 0.6920623686637899 1.5274492035222695E-4 3.5522074500517895E-6 1.0656622350155369E-4 1.5807323152730463E-4 44-45 0.9406121000476386 1.5807323152730463E-4 3.5522074500517895E-6 1.0656622350155369E-4 1.634015427023823E-4 46-47 1.2459545241429155 1.651776464274082E-4 3.5522074500517895E-6 1.0834232722657959E-4 1.669537501524341E-4 48-49 1.6193039598694337 1.7938647622761536E-4 3.5522074500517895E-6 1.1189453467663136E-4 1.7228206132751178E-4 50-51 2.043895764166674 1.9714751347787432E-4 3.5522074500517895E-6 1.2255115702678674E-4 1.7405816505253768E-4 52-53 2.497967338091894 2.0780413582802967E-4 3.5522074500517895E-6 1.2787946820186442E-4 1.7761037250258946E-4 54-55 2.9941716445821784 2.1490855072813327E-4 3.5522074500517895E-6 1.2965557192689032E-4 1.7761037250258946E-4 56-57 3.55498882848518 2.1668465445315915E-4 3.5522074500517895E-6 1.4386440172709747E-4 1.918192023027966E-4 58-59 4.1813371354267375 2.2378906935326272E-4 3.5522074500517895E-6 1.616254389773564E-4 2.060280321030038E-4 60-61 4.859402030633598 2.3089348425336633E-4 3.5522074500517895E-6 1.7228206132751178E-4 2.060280321030038E-4 62-63 5.567111873114866 2.344456917034181E-4 3.5522074500517895E-6 1.8649089112771894E-4 2.2378906935326272E-4 64-65 6.299180978184864 2.5220672895367703E-4 3.5522074500517895E-6 1.9004309857777074E-4 2.2911738052834043E-4 66-67 7.074182062496188 2.664155587538842E-4 3.5522074500517895E-6 1.9892361720290022E-4 2.308934842533663E-4 68-69 7.897786225242847 2.735199736539878E-4 3.5522074500517895E-6 2.060280321030038E-4 2.308934842533663E-4 70-71 8.761810956563643 2.8062438855409134E-4 3.5522074500517895E-6 2.0958023955305557E-4 2.344456917034181E-4 72-73 9.64508510005627 2.8772880345419495E-4 3.5522074500517895E-6 2.0958023955305557E-4 2.468784177785994E-4 74-75 10.542518342444804 2.9128101090424675E-4 3.5522074500517895E-6 2.0958023955305557E-4 2.5575893640372884E-4 76-77 11.457629145208521 3.001615295293762E-4 3.5522074500517895E-6 2.1668465445315915E-4 2.6463945502885834E-4 78-79 12.400218048702115 3.0726594442947975E-4 3.5522074500517895E-6 2.2023686190321092E-4 2.735199736539878E-4 80-81 13.363477247347557 3.0904204815450565E-4 3.5522074500517895E-6 2.2378906935326272E-4 2.8595269972916905E-4 82-83 14.33957767592494 3.1437035932958335E-4 3.5522074500517895E-6 2.2378906935326272E-4 2.8772880345419495E-4 84-85 15.319992260450409 3.1969867050466106E-4 3.5522074500517895E-6 2.2734127680331453E-4 2.8772880345419495E-4 86-87 16.312573155492302 3.410119152049718E-4 3.5522074500517895E-6 2.308934842533663E-4 2.9305711462927265E-4 88 17.064364116324988 3.587729524552307E-4 3.5522074500517895E-6 2.308934842533663E-4 3.054898407044539E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 176030 0.0 84.3181 8 AGTGGTA 177950 0.0 83.578896 5 GTGGTAT 178960 0.0 83.064896 6 TGGTATC 178365 0.0 83.003174 7 GTATCAA 178525 0.0 82.99119 9 AAGCAGT 182910 0.0 81.801384 1 GCAGTGG 186905 0.0 80.11165 3 CAGTGGT 187445 0.0 79.27767 4 AGCAGTG 193160 0.0 77.53541 2 CAACGCA 173560 0.0 44.305824 12-13 ATCAACG 174335 0.0 44.19728 10-11 ACGCAGA 176525 0.0 43.56477 14-15 AGAGTAC 175215 0.0 43.265163 18-19 TCAACGC 172860 0.0 43.022293 12-13 GAGTACT 170660 0.0 42.63832 20-21 AACGCAG 175010 0.0 42.602867 14-15 AGTACTT 179115 0.0 41.940304 20-21 CGCAGAG 178785 0.0 41.589664 16-17 TATCAAC 178510 0.0 41.496975 10-11 GTACTTT 180065 0.0 40.412502 22-23 >>END_MODULE