FastQCFastQC Report
Thu 26 May 2016
SRR1660737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28151509
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10891673.8689471317505575No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT394260.14004933092574184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1498100.081.97518
AGTGGTA1501800.080.947855
GTGGTAT1508550.080.638566
AAGCAGT1470000.080.572061
TGGTATC1523300.080.526237
GTATCAA1524150.080.4877859
GCAGTGG1552400.078.239243
AGCAGTG1594100.075.91292
CAGTGGT1598250.075.761274
CAACGCA1464000.043.70558512-13
ATCAACG1479750.043.37842610-11
ACGCAGA1495800.042.85503414-15
AGAGTAC1511250.042.3116118-19
TCAACGC1458050.041.96684612-13
AACGCAG1479650.041.41780514-15
GAGTACT1466800.041.4019720-21
AGTACTT1555050.040.84093520-21
CGCAGAG1514450.040.4941216-17
TATCAAC1525650.040.15814210-11
GTACTTT1561050.038.91320422-23
Produced by FastQC (version 0.11.5)