FastQCFastQC Report
Thu 26 May 2016
SRR1660736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24961665
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10681914.2793259183632175No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT288200.11545704182793896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1387400.083.32528
GTGGTAT1408150.082.232816
AGTGGTA1411750.082.09345
GTATCAA1407400.082.060519
TGGTATC1407250.081.978537
AAGCAGT1466300.079.464471
GCAGTGG1487650.078.300013
CAGTGGT1495150.077.40014
AGCAGTG1547250.075.430632
CAACGCA1356350.044.15946612-13
ATCAACG1369400.043.88202310-11
ACGCAGA1382250.043.40915314-15
AGAGTAC1385200.042.7871218-19
TCAACGC1352150.042.7825912-13
AACGCAG1369900.042.3368214-15
GAGTACT1350500.042.05513820-21
CGCAGAG1400850.041.379416-17
AGTACTT1421200.041.32236520-21
TATCAAC1412400.040.8488710-11
GTACTTT1431700.039.60707522-23
Produced by FastQC (version 0.11.5)