FastQCFastQC Report
Thu 26 May 2016
SRR1660736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24961665
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10731704.299272504458337No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT309900.12415037218070188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1443350.083.422328
GTGGTAT1448900.082.4160466
AGTGGTA1454950.082.157245
TGGTATC1467650.082.032827
GTATCAA1469900.081.829129
AAGCAGT1444900.080.566261
GCAGTGG1497200.079.826893
CAGTGGT1545800.077.2436454
AGCAGTG1555850.076.551412
CAACGCA1409650.044.11851512-13
ATCAACG1428100.043.64549310-11
ACGCAGA1439700.043.3005314-15
AGAGTAC1457350.042.7345418-19
TCAACGC1407950.042.66821712-13
AACGCAG1424450.042.238414-15
GAGTACT1422800.042.00758720-21
AGTACTT1489850.041.51914620-21
CGCAGAG1455950.041.31992716-17
TATCAAC1471250.040.86114510-11
GTACTTT1496250.039.91435622-23
Produced by FastQC (version 0.11.5)