FastQCFastQC Report
Thu 26 May 2016
SRR1660735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24456627
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13959185.707729033934237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276310.11297960262467918No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT267300.10929552959204063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1761750.086.331298
GTGGTAT1778800.085.623556
AGTGGTA1780600.085.581245
TGGTATC1774400.085.465777
GTATCAA1790700.084.877369
AAGCAGT1826800.083.764681
GCAGTGG1839400.083.307833
CAGTGGT1883250.080.815834
AGCAGTG1897700.080.741052
CAACGCA1735250.044.8865412-13
ATCAACG1746800.044.72306410-11
ACGCAGA1759350.044.29712714-15
TCAACGC1735250.043.9045612-13
AGAGTAC1761650.043.6363818-19
AACGCAG1751300.043.60149414-15
GAGTACT1729150.043.17986720-21
CGCAGAG1765250.043.16202516-17
TATCAAC1777900.042.71146410-11
AGTACTT1825900.041.81988520-21
GTACTTT1805000.041.2982822-23
Produced by FastQC (version 0.11.5)