##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660735_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24456627 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83361115169316 34.0 31.0 34.0 30.0 34.0 2 31.956466155369668 34.0 31.0 34.0 30.0 34.0 3 32.01835375744987 34.0 31.0 34.0 30.0 34.0 4 35.422808386454925 37.0 35.0 37.0 33.0 37.0 5 35.39078990737357 37.0 35.0 37.0 33.0 37.0 6 35.47093840863664 37.0 35.0 37.0 33.0 37.0 7 35.419780413709546 37.0 35.0 37.0 33.0 37.0 8 35.432470471091534 37.0 35.0 37.0 33.0 37.0 9 37.11004518325442 39.0 37.0 39.0 33.0 39.0 10-11 37.0413760041399 39.0 37.0 39.0 33.0 39.0 12-13 37.07153484411403 39.0 37.0 39.0 33.0 39.0 14-15 38.44737358917074 40.0 38.0 41.0 34.0 41.0 16-17 38.4250858264306 40.0 38.0 41.0 33.0 41.0 18-19 38.38861129541698 40.0 38.0 41.0 33.5 41.0 20-21 38.26687075449938 40.0 38.0 41.0 33.5 41.0 22-23 38.08899510140952 40.0 38.0 41.0 33.0 41.0 24-25 38.074905586939686 40.0 38.0 41.0 33.0 41.0 26-27 38.06243436594916 40.0 38.0 41.0 33.0 41.0 28-29 37.98126456685952 40.0 38.0 41.0 33.0 41.0 30-31 37.89206626899122 40.0 38.0 41.0 32.5 41.0 32-33 37.766446084327164 40.0 38.0 41.0 32.0 41.0 34-35 37.609539369431445 40.0 37.5 41.0 31.5 41.0 36-37 37.41862404001991 40.0 37.0 41.0 31.0 41.0 38-39 36.98296007867316 39.5 35.0 41.0 30.5 41.0 40-41 36.91993564770809 39.5 35.0 41.0 30.5 41.0 42-43 36.92496945715368 40.0 35.0 41.0 30.0 41.0 44-45 36.923434883314044 40.0 35.0 41.0 30.0 41.0 46-47 37.01541101722654 40.0 35.0 41.0 31.0 41.0 48-49 36.90716702266424 40.0 35.0 41.0 30.0 41.0 50-51 35.541206275092634 38.5 34.0 40.0 28.0 40.5 52-53 35.69550817453282 38.5 35.0 39.5 28.0 40.5 54-55 36.0640617980558 39.0 35.0 41.0 27.0 41.0 56-57 35.68200369576721 39.0 35.0 41.0 24.5 41.0 58-59 35.494768636738016 39.0 35.0 41.0 24.5 41.0 60-61 35.279512726755 39.0 35.0 41.0 24.0 41.0 62-63 35.0572373083173 38.5 35.0 41.0 23.5 41.0 64-65 34.808096656174214 38.0 34.0 40.5 23.0 41.0 66-67 34.55018576355603 37.0 34.0 40.0 23.0 41.0 68-69 34.282148474521854 37.0 34.0 40.0 23.0 41.0 70-71 33.98678035200848 36.0 34.0 39.0 23.0 41.0 72-73 33.64149371456661 36.0 33.5 39.0 23.0 41.0 74-75 33.25598129292318 35.0 33.0 38.5 22.0 40.5 76-77 32.855328373777795 35.0 33.0 37.0 20.5 39.5 78-79 32.48291577575272 35.0 33.0 37.0 20.0 39.0 80-81 32.158794710325346 35.0 32.5 36.5 20.0 39.0 82-83 31.839272725547964 35.0 32.0 36.0 19.0 37.5 84-85 31.549204230002772 35.0 32.0 35.5 18.0 37.0 86-87 31.3309362529837 35.0 32.0 35.0 18.0 37.0 88-89 31.12230333316201 35.0 32.0 35.0 18.0 36.0 90-91 30.944588086492878 35.0 31.0 35.0 17.0 36.0 92-93 30.789301832178246 35.0 31.0 35.0 17.0 36.0 94-95 30.605179099309158 35.0 31.0 35.0 15.0 35.5 96-97 30.46550632268301 35.0 31.0 35.0 15.0 35.0 98-99 30.358432522195315 34.5 31.0 35.0 15.0 35.0 100 30.27436273203169 34.0 30.0 35.0 14.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5276586480734871 1101 2 0.5511048890699008 1101 3 0.5368641895263124 1101 4 0.4691564212228556 1101 5 0.49012785195748165 1101 6 0.4534095600215835 1101 7 0.46600842246076013 1101 8 0.4649352319894575 1101 9 0.5452772798760677 1101 10-11 0.5548012094176755 1101 12-13 0.5504667896544575 1101 14-15 0.6458539231569702 1101 16-17 0.6206517894128893 1101 18-19 0.6286143889827329 1101 20-21 0.6840888214577348 1101 22-23 0.727068828069541 1101 24-25 0.7333192523926115 1101 26-27 0.6802675660096682 1101 28-29 0.6580452171384792 1101 30-31 0.6553328476469531 1101 32-33 0.64253266005867 1101 34-35 0.6637754181164865 1101 36-37 0.5627237913826804 1101 38-39 0.6018237171912375 1101 40-41 0.5873344444043482 1101 42-43 0.6302212794536075 1101 44-45 0.6419839536917209 1101 46-47 0.6815963003278966 1101 48-49 0.7172650849718494 1101 50-51 0.6297051507229767 1101 52-53 0.6695160429781382 1101 54-55 0.7350400786513092 1101 56-57 0.7135776667642517 1101 58-59 0.6070544310682706 1101 60-61 0.5874739479480695 1101 62-63 0.5419760171458705 1101 64-65 0.5306393552677307 1101 66-67 0.5343571574809829 1101 68-69 0.5487708910120759 1101 70-71 0.5367051554967546 1101 72-73 0.5023100364342881 1101 74-75 0.46140882242520576 1101 76-77 0.4887064526134566 1101 78-79 0.491922959348571 1101 80-81 0.46566130518244364 1101 82-83 0.4670837962423313 1101 84-85 0.43707109294174273 1101 86-87 0.41761782487876076 1101 88-89 0.3849066498196656 1101 90-91 0.37642097537819197 1101 92-93 0.3709810292362299 1101 94-95 0.3635447926408162 1101 96-97 0.35535481479322684 1101 98-99 0.30655655668494575 1101 100 0.3010814415807417 1102 1 0.4753878717057951 1102 2 0.5121119195033721 1102 3 0.5088355505918969 1102 4 0.4332996993671543 1102 5 0.42798548201818676 1102 6 0.3817826573773857 1102 7 0.4127079153316586 1102 8 0.44581694373353287 1102 9 0.509209245997404 1102 10-11 0.5125807913865756 1102 12-13 0.5209874678971289 1102 14-15 0.6069719394932065 1102 16-17 0.6041685062800042 1102 18-19 0.5564596436778828 1102 20-21 0.5692487688493486 1102 22-23 0.5932596571404432 1102 24-25 0.5842267117091424 1102 26-27 0.5661755500612244 1102 28-29 0.5693672268620702 1102 30-31 0.5435536482450161 1102 32-33 0.573457396124283 1102 34-35 0.5654055655392298 1102 36-37 0.5811243609240648 1102 38-39 0.5794292848062454 1102 40-41 0.5429639002604745 1102 42-43 0.5776370201160361 1102 44-45 0.5773907367787317 1102 46-47 0.6372677180547086 1102 48-49 0.6200394447055118 1102 50-51 0.5974476963320967 1102 52-53 0.6348849738898465 1102 54-55 0.6772850734424836 1102 56-57 0.6531791060310184 1102 58-59 0.5585546180342291 1102 60-61 0.5506660962358509 1102 62-63 0.5560782498362684 1102 64-65 0.5324590951419523 1102 66-67 0.5115436425529225 1102 68-69 0.5165244667800479 1102 70-71 0.478715458702176 1102 72-73 0.4575448298911553 1102 74-75 0.42041590551561825 1102 76-77 0.42470050788342206 1102 78-79 0.4213593541404137 1102 80-81 0.38029738909524013 1102 82-83 0.373786433995992 1102 84-85 0.35391745207784453 1102 86-87 0.3568838725358745 1102 88-89 0.34079998644109466 1102 90-91 0.31534058362984396 1102 92-93 0.27481930017237133 1102 94-95 0.34422148170417444 1102 96-97 0.33193701859173075 1102 98-99 0.24706882533980234 1102 100 0.24288408714264875 1103 1 0.41405051769904944 1103 2 0.42262975124685553 1103 3 0.4467371712233543 1103 4 0.3796718439901028 1103 5 0.39161969878269787 1103 6 0.3559161095027079 1103 7 0.35557190002354844 1103 8 0.4018346917915494 1103 9 0.45905129982435966 1103 10-11 0.43356715621572306 1103 12-13 0.46448163375342943 1103 14-15 0.5518561116304213 1103 16-17 0.5123467543886733 1103 18-19 0.5120272146346849 1103 20-21 0.5716240674201885 1103 22-23 0.5560406107818707 1103 24-25 0.5871801640834136 1103 26-27 0.5543914040815068 1103 28-29 0.5374716372756438 1103 30-31 0.5304309472276287 1103 32-33 0.4821005057900223 1103 34-35 0.5063167358286975 1103 36-37 0.4843290150406858 1103 38-39 0.5265674521760246 1103 40-41 0.47998163841973707 1103 42-43 0.5106715216084226 1103 44-45 0.49483469104008293 1103 46-47 0.5414601085971782 1103 48-49 0.5715674547562131 1103 50-51 0.5047923675231019 1103 52-53 0.48356503443877585 1103 54-55 0.5801915036969589 1103 56-57 0.6223343028479462 1103 58-59 0.5266022001055646 1103 60-61 0.5468885336060438 1103 62-63 0.49771929148370475 1103 64-65 0.5009081680000165 1103 66-67 0.4817002324051174 1103 68-69 0.4609947266826495 1103 70-71 0.45705697962893765 1103 72-73 0.39350336465401625 1103 74-75 0.45724855362671235 1103 76-77 0.4502562293201535 1103 78-79 0.41145101482374713 1103 80-81 0.36324341985081077 1103 82-83 0.3453007129784318 1103 84-85 0.3157384080537682 1103 86-87 0.2987268761889652 1103 88-89 0.2960987155410457 1103 90-91 0.29155272970390556 1103 92-93 0.32097280025269015 1103 94-95 0.31745293201758784 1103 96-97 0.30967637207972487 1103 98-99 0.3032520946463748 1103 100 0.3110692334757701 1104 1 0.38684020569597166 1104 2 0.4293386999493656 1104 3 0.4280767364324447 1104 4 0.36753046648097154 1104 5 0.3666532768261064 1104 6 0.3060834995036501 1104 7 0.31043261289892143 1104 8 0.3496198413449676 1104 9 0.3884267521334124 1104 10-11 0.39368082807281013 1104 12-13 0.3921429411615236 1104 14-15 0.48504463361756933 1104 16-17 0.4616932753224745 1104 18-19 0.4488802280740316 1104 20-21 0.3933955340303612 1104 22-23 0.4553053117822543 1104 24-25 0.4528361658832196 1104 26-27 0.41649518367166394 1104 28-29 0.3911828570514899 1104 30-31 0.3840713319344289 1104 32-33 0.3897553501068032 1104 34-35 0.3825112142361391 1104 36-37 0.37044129097601797 1104 38-39 0.38436895095263424 1104 40-41 0.3609001836655992 1104 42-43 0.3525042577287749 1104 44-45 0.3819396473017136 1104 46-47 0.4360016941847178 1104 48-49 0.45149743090049554 1104 50-51 0.3657450226469692 1104 52-53 0.4059237457218572 1104 54-55 0.5107329249667387 1104 56-57 0.5016545495084728 1104 58-59 0.41530231565824494 1104 60-61 0.4614735094898421 1104 62-63 0.43078360629799306 1104 64-65 0.4266334730326946 1104 66-67 0.4283260237651234 1104 68-69 0.35939526055050663 1104 70-71 0.3635269033668109 1104 72-73 0.374216276691989 1104 74-75 0.3847580809747697 1104 76-77 0.4053800944104182 1104 78-79 0.389788885671031 1104 80-81 0.34009817817183574 1104 82-83 0.3077405567899554 1104 84-85 0.28167774377327603 1104 86-87 0.2816494054227796 1104 88-89 0.27658773192132813 1104 90-91 0.23870326814946807 1104 92-93 0.26488181024997104 1104 94-95 0.3536605916471274 1104 96-97 0.3048210105630851 1104 98-99 0.2700753436799701 1104 100 0.22342025801942 1105 1 0.2789916583097245 1105 2 0.3227835865794937 1105 3 0.31924295513019985 1105 4 0.27807949408303756 1105 5 0.28536377346655684 1105 6 0.26201081802905435 1105 7 0.25853032873469317 1105 8 0.2693399592023056 1105 9 0.2864165805789227 1105 10-11 0.3017615480482192 1105 12-13 0.30035896491994407 1105 14-15 0.3645834908330201 1105 16-17 0.31628048817177046 1105 18-19 0.3301945151216046 1105 20-21 0.3611780397926836 1105 22-23 0.40623337745041255 1105 24-25 0.43447845493652437 1105 26-27 0.37963344180828784 1105 28-29 0.3814631800525774 1105 30-31 0.39691206567127324 1105 32-33 0.3770469084162258 1105 34-35 0.37848222866046655 1105 36-37 0.36910435793107155 1105 38-39 0.38855204616696426 1105 40-41 0.3928715017450912 1105 42-43 0.43561895025757025 1105 44-45 0.44601900250109594 1105 46-47 0.4679395350988571 1105 48-49 0.47125365392957264 1105 50-51 0.35997677876307677 1105 52-53 0.4026851439962158 1105 54-55 0.4755299539219138 1105 56-57 0.5135088268000914 1105 58-59 0.42904765199445904 1105 60-61 0.42535709432242896 1105 62-63 0.4380081548170267 1105 64-65 0.43007907628548736 1105 66-67 0.4250592885254605 1105 68-69 0.4335412081927572 1105 70-71 0.42177234232931227 1105 72-73 0.3947655577938818 1105 74-75 0.34930433034990216 1105 76-77 0.3529608558473072 1105 78-79 0.3685141959907057 1105 80-81 0.33885906856057346 1105 82-83 0.3445475111253735 1105 84-85 0.32843687044449155 1105 86-87 0.2884158554564351 1105 88-89 0.31472557281591307 1105 90-91 0.31446432170126926 1105 92-93 0.2847090655837512 1105 94-95 0.31720407363986425 1105 96-97 0.3122918742730505 1105 98-99 0.2878591984433747 1105 100 0.27668085558699573 1106 1 0.2095747727661852 1106 2 0.24616312979715715 1106 3 0.24217824255910614 1106 4 0.22489899188082063 1106 5 0.2285143060921726 1106 6 0.18858944720292214 1106 7 0.21376465871744443 1106 8 0.22613842270304474 1106 9 0.2301380823987742 1106 10-11 0.19206202430977726 1106 12-13 0.22273830289366714 1106 14-15 0.29929421003537726 1106 16-17 0.26573021723430656 1106 18-19 0.24355886819301986 1106 20-21 0.2550900440626407 1106 22-23 0.2357896601384084 1106 24-25 0.2545590200223131 1106 26-27 0.29760801573704754 1106 28-29 0.28794958373552504 1106 30-31 0.2565346318402675 1106 32-33 0.26890260415031975 1106 34-35 0.2878401948218752 1106 36-37 0.23660940393278906 1106 38-39 0.23743659980853238 1106 40-41 0.23648058647848558 1106 42-43 0.27533122190043713 1106 44-45 0.27176034985058806 1106 46-47 0.2842097679423503 1106 48-49 0.3291088279960448 1106 50-51 0.19638560919464254 1106 52-53 0.21555584421358276 1106 54-55 0.307438141769083 1106 56-57 0.2689592730406929 1106 58-59 0.19330424347682396 1106 60-61 0.2304281588599011 1106 62-63 0.20178935191860603 1106 64-65 0.18168668269819221 1106 66-67 0.2169120537047604 1106 68-69 0.20720244985402303 1106 70-71 0.23869696479758318 1106 72-73 0.23869429585056423 1106 74-75 0.20522075208160828 1106 76-77 0.17185617353722904 1106 78-79 0.21914830384628203 1106 80-81 0.17553222341961572 1106 82-83 0.20040662039020063 1106 84-85 0.1796907315853673 1106 86-87 0.14623238061601285 1106 88-89 0.18152259973734886 1106 90-91 0.1266353708896233 1106 92-93 0.12560883005700063 1106 94-95 0.21603299608475623 1106 96-97 0.19979687809158975 1106 98-99 0.1411476209844622 1106 100 0.12065952761810905 1107 1 0.2306368954298499 1107 2 0.2685623617752064 1107 3 0.25460665760420653 1107 4 0.2781564926663975 1107 5 0.27289605345571744 1107 6 0.24790838426255135 1107 7 0.24849722368291083 1107 8 0.28226039271334 1107 9 0.3211317845645425 1107 10-11 0.29853833247936734 1107 12-13 0.28368364339652885 1107 14-15 0.32739591075689845 1107 16-17 0.3190068069420491 1107 18-19 0.2757591823596215 1107 20-21 0.317256470220201 1107 22-23 0.35414380814689395 1107 24-25 0.3536425136041359 1107 26-27 0.3307424098383578 1107 28-29 0.32036297604168595 1107 30-31 0.3243098350250264 1107 32-33 0.29159192853938265 1107 34-35 0.33360986548552063 1107 36-37 0.3397299567527625 1107 38-39 0.36932460736730377 1107 40-41 0.35173993757567246 1107 42-43 0.3887653174681702 1107 44-45 0.3622416646330109 1107 46-47 0.3556554558709166 1107 48-49 0.4226723700719148 1107 50-51 0.3513375683081179 1107 52-53 0.35902161069471106 1107 54-55 0.40667764296832587 1107 56-57 0.48238795492490283 1107 58-59 0.3902753274617723 1107 60-61 0.4416616778922915 1107 62-63 0.39835852079272627 1107 64-65 0.39420987977992894 1107 66-67 0.41070035012725725 1107 68-69 0.3893712338709818 1107 70-71 0.3736116092758337 1107 72-73 0.37900706067235745 1107 74-75 0.39023772701957427 1107 76-77 0.3702378453832793 1107 78-79 0.36088786498321923 1107 80-81 0.31479417617158134 1107 82-83 0.33344157314799716 1107 84-85 0.36367863766385256 1107 86-87 0.35727339057918783 1107 88-89 0.34634705735717475 1107 90-91 0.33185810132487603 1107 92-93 0.3534567029955973 1107 94-95 0.39257557724018355 1107 96-97 0.42365771414792874 1107 98-99 0.3860137420146259 1107 100 0.416038648713176 1108 1 0.14220362299815648 1108 2 0.19188393977629303 1108 3 0.18415501743679386 1108 4 0.16171405064689992 1108 5 0.18325232231386224 1108 6 0.12562247887833422 1108 7 0.1435689521603578 1108 8 0.17277571917177426 1108 9 0.17304804918003924 1108 10-11 0.134124544443587 1108 12-13 0.15404652980028288 1108 14-15 0.20944510396635252 1108 16-17 0.17887426936731998 1108 18-19 0.156442805088723 1108 20-21 0.14496409333069948 1108 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-1.2320680376727111 2316 86-87 -1.3932431226246216 2316 88-89 -1.385186680150845 2316 90-91 -1.3267924011946164 2316 92-93 -1.150624984094538 2316 94-95 -1.3632208017527603 2316 96-97 -1.5420958985011737 2316 98-99 -1.3891558457152513 2316 100 -1.2118060766405705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12558.0 3 3175.0 4 2191.0 5 1861.0 6 3073.0 7 15700.0 8 30746.0 9 35528.0 10 36682.0 11 35355.0 12 34821.0 13 37009.0 14 42471.0 15 50822.0 16 62388.0 17 76567.0 18 85105.0 19 94968.0 20 108395.0 21 132663.0 22 171716.0 23 249880.0 24 371906.0 25 468369.0 26 464486.0 27 387512.0 28 343743.0 29 338142.0 30 379693.0 31 457861.0 32 567344.0 33 735123.0 34 1010671.0 35 1537869.0 36 2671018.0 37 5012833.0 38 6387229.0 39 1995514.0 40 3640.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.22549999070125 33.71473550300695 27.905514645372836 22.154249860918974 2 20.67773171900032 34.17164184924509 26.692465784631274 18.458160647123314 3 26.090633628369037 25.41567612295536 26.97964400675097 21.514046241924635 4 19.369822921955667 27.577420341586183 24.81748897798579 28.235267758472354 5 21.316073954661455 32.84941701339013 26.223372322656473 19.611136709291944 6 28.196971913417613 27.173685167438027 25.607525086500445 19.021817832643915 7 20.803801942448004 25.922654612060082 32.74484759536025 20.52869585013167 8 27.210870788451462 26.65803076348061 25.68748665653867 20.443611791529253 9 26.554725308919984 25.484216215330946 27.167717554188947 20.79334092156012 10-11 20.377224570537525 29.98809765661688 29.278839425919816 20.355838346925776 12-13 20.2789722656248 26.676899723582586 29.306989148530988 23.737138862261627 14-15 20.245917601413637 33.298863284789874 26.081422279611566 20.373796834184922 16-17 23.65320805938324 26.47736912584371 26.143971245748276 23.725451569024777 18-19 20.256381763163347 29.85744986079647 26.170863473860845 23.715304902179334 20-21 23.60375353409534 29.960616139580804 26.116848646700692 20.318781679623164 22-23 23.620667324163914 26.650289878683758 29.395983804145526 20.33305899300681 24-25 20.36827001324463 29.789447764418416 25.996387649496288 23.845894572840667 26-27 20.37050159511732 26.51974962263715 32.74634560091613 20.3634031813294 28-29 20.34787370324805 26.64143238111703 32.70837609121228 20.302317824422637 30-31 20.312095393590983 26.56143014173505 32.71208505449504 20.41438941017893 32-33 20.43003035172729 26.694801149790703 32.508090362543776 20.36707813593823 34-35 20.453988135358244 26.830053228512746 32.388012825794554 20.32794581033445 36-37 20.3933890666334 26.76095584525127 32.45255048364298 20.393104604472352 38-39 20.42386669874166 26.76176062227391 32.4650690770251 20.34930360195933 40-41 20.441409126438025 26.815035302162038 32.44301251064533 20.30054306075461 42-43 20.446892944358083 26.846774734016503 32.39756078860029 20.308771533025126 44-45 20.451658288068884 26.911497441576966 32.350925592765364 20.28591867758878 46-47 20.452622849574734 27.006476502459908 32.29021796020507 20.250682687760285 48-49 20.523095124669393 27.033176519972784 32.160294667216895 20.283433688140928 50-51 20.53871419267116 27.085302781697063 32.10167373488613 20.27430929074565 52-53 20.65180161890292 27.225768438596955 31.823748248153194 20.298681694346936 54-55 21.015623692095637 27.507765715717397 31.039957339063374 20.43665325312359 56-57 21.494584758045132 28.070836528770066 29.86928276062157 20.565295952563236 58-59 21.616472880857568 28.592782474365908 29.198544550113215 20.59220009466331 60-61 21.706289175832516 28.84722899346115 28.888571355246455 20.557910475459877 62-63 21.755339140725663 29.003863326445966 28.727118216839603 20.513679315988764 64-65 21.854894557201156 29.043697348754137 28.68266286586848 20.418745228176224 66-67 21.947972031453293 28.850273672719958 28.862483802898982 20.339270492927767 68-69 22.048399076084486 28.673395079184072 29.051204009698683 20.227001835032763 70-71 22.16163998001111 28.556265594827977 29.16844178759256 20.113652637568357 72-73 22.30596802031502 28.472956185646453 29.125693376688133 20.09538241735039 74-75 22.51366421745332 28.571782652862836 28.932687829916144 19.981865299767694 76-77 22.80574141299209 28.712818375593336 28.599549166401577 19.881891045012992 78-79 23.073168746879762 28.853775076314324 28.247372368868962 19.825683807936954 80-81 23.322138994123836 29.03607994244443 27.90350883602101 19.738272227410718 82-83 23.549641205857448 29.20479448819368 27.530902108998788 19.71466219695008 84-85 23.703064997921174 29.240137437288542 27.329029731132557 19.727767833657726 86-87 23.924128561647652 29.220744048326996 27.18624323733771 19.668884152687642 88-89 24.15538979177338 29.243806942459504 27.022658336245353 19.578144929521756 90-91 24.377873433639248 29.17521609838397 26.882199803185376 19.564710664791406 92-93 24.561814453279233 29.17312054009664 26.802651174599674 19.462413832024453 94-95 24.736961195979113 29.167131627399822 26.681562606253888 19.414344570367174 96-97 24.870389147398665 29.142863564838674 26.593186876515812 19.393560411246852 98-99 25.058670636613456 29.146948758439102 26.468964375448188 19.325416229499258 100 25.145570504806802 29.158485033496014 26.364892092503904 19.331052369193277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33679.0 1 24368.5 2 12814.5 3 9991.5 4 9342.5 5 9336.5 6 9323.5 7 9339.0 8 9573.5 9 9870.0 10 10493.0 11 13114.0 12 105638.5 13 130952.0 14 64811.0 15 67605.5 16 76248.5 17 85395.5 18 93836.5 19 101766.0 20 111927.0 21 127150.5 22 146491.0 23 168078.0 24 191061.0 25 218172.5 26 250894.0 27 289180.5 28 336265.5 29 380978.5 30 415785.5 31 450218.5 32 488804.0 33 524925.0 34 554128.5 35 592357.0 36 640574.0 37 675958.5 38 702970.5 39 723220.0 40 732724.0 41 745585.0 42 769566.5 43 783701.0 44 792076.0 45 807786.5 46 816083.0 47 821450.5 48 813394.0 49 794280.5 50 783077.0 51 771240.0 52 750199.0 53 716099.0 54 668855.5 55 619673.5 56 571527.5 57 520028.0 58 463208.5 59 399441.0 60 330683.0 61 268435.0 62 212678.0 63 160204.0 64 118025.5 65 86369.5 66 62682.0 67 46347.5 68 35139.5 69 26699.0 70 20660.5 71 16690.0 72 14096.0 73 11494.5 74 9360.5 75 8048.5 76 6316.0 77 4434.5 78 3273.5 79 2631.5 80 2104.0 81 1725.5 82 1401.5 83 1143.0 84 953.0 85 782.5 86 691.5 87 602.0 88 512.5 89 450.5 90 388.0 91 344.5 92 310.0 93 258.5 94 224.0 95 183.0 96 139.0 97 119.0 98 92.0 99 69.0 100 50.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4028519550140745 2 0.38450518953410867 3 0.1683142977974845 4 0.17483604750565154 5 0.2734350898020402 6 0.1020623162793463 7 0.1362493691382708 8 0.141282769696737 9 0.1342253778495293 10-11 0.13094610307463903 12-13 0.1329476055712834 14-15 0.1389194020908934 16-17 0.1433394719558016 18-19 0.15490893327195118 20-21 0.1361491917916563 22-23 0.09845388736557989 24-25 0.0938457294213139 26-27 0.0882807755950974 28-29 0.09811451104847779 30-31 0.1025918251114514 32-33 0.10384915303324534 34-35 0.10446248372680336 36-37 0.10040223453544922 38-39 0.09935752792075538 40-41 0.10039814566415883 42-43 0.06316897256518653 44-45 0.0661620263497497 46-47 0.08453945836439343 48-49 0.08889410628865542 50-51 0.0982310438802538 52-53 0.10331351089420467 54-55 0.10568096737133867 56-57 0.10612665434199083 58-59 0.10561758986633767 60-61 0.10332577750807584 62-63 0.10788486899685718 64-65 0.11479097260632058 66-67 0.12343484651419839 68-69 0.12859500208266658 70-71 0.12700443115070612 72-73 0.11369515510049688 74-75 0.11129294321739461 76-77 0.11309000214951964 78-79 0.11203098448530945 80-81 0.10621047620344376 82-83 0.10934050717623489 84-85 0.11521008191358521 86-87 0.12888122307299368 88-89 0.13678296684166627 90-91 0.14380355884726048 92-93 0.14279769650982532 94-95 0.1329762276703161 96-97 0.12916335519203037 98-99 0.1244611532080855 100 0.11835237950024752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4456627E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.2962087009492 #Duplication Level Percentage of deduplicated Percentage of total 1 76.11298044218712 32.95403486076198 2 11.830579564675183 10.24438483770723 3 4.224772089623506 5.487498423188537 4 2.0613060803863292 3.569869530117684 5 1.279267347916475 2.769371303985075 6 0.8380852668693549 2.177154877413978 7 0.5806714088161948 1.7598609351946115 8 0.43149042739208665 1.4945519677463641 9 0.3441481238666146 1.3410278095472157 >10 1.9760163298543563 15.95506694957393 >50 0.20324666517244938 6.224059485962737 >100 0.1149343645687769 9.086518982183549 >500 0.0018885072835559719 0.47512494890320156 >1k 5.662540574771808E-4 0.4838501289380006 >5k 1.8850932202567323E-5 0.05922565867933765 >10k+ 2.8276398303145333E-5 5.9183993000966595 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1395918 5.707729033934237 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27631 0.11297960262467918 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 26730 0.10929552959204063 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.1075421806940097E-4 0.0 0.0 4.088871290386855E-6 4.088871290386855E-6 2 3.189319606501747E-4 0.0 0.0 4.088871290386855E-6 8.17774258077371E-6 3 3.311985745213353E-4 0.0 0.0 1.2266613871160565E-5 8.17774258077371E-6 4 3.3937631710210896E-4 8.17774258077371E-6 0.0 1.2266613871160565E-5 1.635548516154742E-5 5 3.434651883924958E-4 1.2266613871160565E-5 0.0 1.2266613871160565E-5 1.635548516154742E-5 6 3.475540596828827E-4 1.635548516154742E-5 0.0 1.635548516154742E-5 1.635548516154742E-5 7 3.475540596828827E-4 2.0444356451934276E-5 0.0 1.635548516154742E-5 2.0444356451934276E-5 8 3.475540596828827E-4 2.8622099032707986E-5 0.0 1.635548516154742E-5 2.0444356451934276E-5 9 3.475540596828827E-4 3.6799841613481696E-5 0.0 2.0444356451934276E-5 2.0444356451934276E-5 10-11 3.475540596828827E-4 4.7022019839448835E-5 0.0 2.453322774232113E-5 2.6577663387514558E-5 12-13 3.5164293097326955E-4 5.519976242022255E-5 0.0 2.6577663387514558E-5 3.271097032309484E-5 14-15 3.577762379088498E-4 6.337750500099625E-5 0.0 3.271097032309484E-5 4.293314854906198E-5 16-17 3.6595398048962354E-4 7.768855451735024E-5 0.0 4.088871290386855E-5 4.7022019839448835E-5 18-19 4.0479825774829867E-4 1.0222178225967137E-4 0.0 4.088871290386855E-5 5.519976242022255E-5 20-21 4.231981785550395E-4 1.0631065355005823E-4 0.0 4.088871290386855E-5 5.724419806541597E-5 22-23 5.008867330723898E-4 1.0631065355005823E-4 0.0 4.088871290386855E-5 6.133306935580283E-5 24-25 7.871077233994696E-4 1.2062170306641223E-4 0.0 4.088871290386855E-5 7.359968322696339E-5 26-27 0.001739814734059607 1.3084388129237936E-4 0.0 4.088871290386855E-5 8.17774258077371E-5 28-29 0.005025222815885445 1.410660595183465E-4 0.0 4.088871290386855E-5 8.791073274331737E-5 30-31 0.013047588287624454 1.4719936645392679E-4 0.0 4.906645548464226E-5 9.199960403370424E-5 32-33 0.02922725198368524 1.4924380209912022E-4 0.0 5.1110891129835684E-5 1.0017734661447795E-4 34-35 0.05783095109558649 1.5946598032508735E-4 0.0 5.3155326775029116E-5 1.0631065355005823E-4 36-37 0.1031172450722661 1.635548516154742E-4 0.0 5.3155326775029116E-5 1.1448839613083195E-4 38-39 0.16872931823345877 1.6559928726066762E-4 0.0 5.3155326775029116E-5 1.185772674212188E-4 40-41 0.25645400733306356 1.7582146548663477E-4 2.0444356451934275E-6 5.3155326775029116E-5 1.247105743567991E-4 42-43 0.3679554829862679 1.8399920806740847E-4 4.088871290386855E-6 5.3155326775029116E-5 1.267550100019925E-4 44-45 0.5087250993360614 2.003546932289559E-4 4.088871290386855E-6 5.519976242022255E-5 1.3084388129237936E-4 46-47 0.6858938479128786 2.0444356451934276E-4 4.088871290386855E-6 5.724419806541597E-5 1.3084388129237936E-4 48-49 0.9031192240859707 2.064880001645362E-4 4.088871290386855E-6 5.724419806541597E-5 1.410660595183465E-4 50-51 1.1540696106621735 2.1671017839050333E-4 4.088871290386855E-6 5.92886337106094E-5 1.4515493080873335E-4 52-53 1.4368477713627477 2.2488792097127703E-4 4.088871290386855E-6 6.133306935580283E-5 1.4719936645392679E-4 54-55 1.7563807960926092 2.2693235661647046E-4 4.088871290386855E-6 6.133306935580283E-5 1.5333267338950708E-4 56-57 2.1207789610562404 2.3511009919724416E-4 4.088871290386855E-6 6.133306935580283E-5 1.6559928726066762E-4 58-59 2.5328901651073963 2.4124340613282445E-4 4.088871290386855E-6 6.133306935580283E-5 1.7377702984144134E-4 60-61 2.987644207845996 2.453322774232113E-4 4.088871290386855E-6 6.133306935580283E-5 1.799103367770216E-4 62-63 3.476366548829485 2.555544556491784E-4 4.088871290386855E-6 6.133306935580283E-5 1.8399920806740847E-4 64-65 3.998206702829462 2.596433269395653E-4 4.088871290386855E-6 6.133306935580283E-5 1.9013251500298877E-4 66-67 4.558087670879553 2.6986550516553244E-4 4.088871290386855E-6 6.337750500099625E-5 1.921769506481822E-4 68-69 5.159767125695625 2.82132119036693E-4 4.088871290386855E-6 6.951081193657654E-5 2.0239912887414933E-4 70-71 5.796619051351604 2.8622099032707984E-4 4.088871290386855E-6 7.564411887215682E-5 2.085324358097296E-4 72-73 6.464391021705487 2.903098616174667E-4 4.088871290386855E-6 8.17774258077371E-5 2.085324358097296E-4 74-75 7.1630401036087274 2.9439873290785357E-4 4.088871290386855E-6 8.791073274331737E-5 2.1875461403569673E-4 76-77 7.892042921536155 3.0257647548862724E-4 4.088871290386855E-6 8.99551683885108E-5 2.2079904968089016E-4 78-79 8.651121432240021 3.209763962953681E-4 4.088871290386855E-6 8.99551683885108E-5 2.3306566355205073E-4 80-81 9.436593607123339 3.3528744581172215E-4 4.088871290386855E-6 9.199960403370424E-5 2.3511009919724416E-4 82-83 10.244636351529586 3.3937631710210896E-4 4.088871290386855E-6 1.0631065355005823E-4 2.453322774232113E-4 84-85 11.074959355597155 3.3937631710210896E-4 4.088871290386855E-6 1.1039952484044508E-4 2.53510020003985E-4 86-87 11.927043332672163 3.434651883924958E-4 4.088871290386855E-6 1.1244396048563851E-4 2.555544556491784E-4 88 12.579118126142252 3.475540596828827E-4 4.088871290386855E-6 1.267550100019925E-4 2.5759889129437185E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 176175 0.0 86.33129 8 GTGGTAT 177880 0.0 85.62355 6 AGTGGTA 178060 0.0 85.58124 5 TGGTATC 177440 0.0 85.46577 7 GTATCAA 179070 0.0 84.87736 9 AAGCAGT 182680 0.0 83.76468 1 GCAGTGG 183940 0.0 83.30783 3 CAGTGGT 188325 0.0 80.81583 4 AGCAGTG 189770 0.0 80.74105 2 CAACGCA 173525 0.0 44.88654 12-13 ATCAACG 174680 0.0 44.723064 10-11 ACGCAGA 175935 0.0 44.297127 14-15 TCAACGC 173525 0.0 43.90456 12-13 AGAGTAC 176165 0.0 43.63638 18-19 AACGCAG 175130 0.0 43.601494 14-15 GAGTACT 172915 0.0 43.179867 20-21 CGCAGAG 176525 0.0 43.162025 16-17 TATCAAC 177790 0.0 42.711464 10-11 AGTACTT 182590 0.0 41.819885 20-21 GTACTTT 180500 0.0 41.29828 22-23 >>END_MODULE