FastQCFastQC Report
Thu 26 May 2016
SRR1660735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24456627
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14041835.741523555149286No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT305120.12475964081228373No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285360.1166800311424793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1835200.086.289078
GTGGTAT1836800.085.447826
TGGTATC1859550.085.138527
AAGCAGT1796550.084.962971
GCAGTGG1845350.084.95983
GTATCAA1861850.084.95049
AGTGGTA1848600.084.93545
AGCAGTG1893500.082.472122
CAGTGGT1939100.080.863084
CAACGCA1802350.044.82538612-13
ATCAACG1811900.044.67590310-11
ACGCAGA1828900.044.2261214-15
TCAACGC1800000.043.834312-13
AACGCAG1816750.043.4897214-15
AGAGTAC1856700.043.4808218-19
GAGTACT1816250.043.1236820-21
CGCAGAG1839800.042.94498416-17
TATCAAC1851500.042.6771510-11
AGTACTT1923450.041.72817220-21
GTACTTT1895450.041.31899322-23
Produced by FastQC (version 0.11.5)