FastQCFastQC Report
Thu 26 May 2016
SRR1660734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26785507
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16193496.045616385009998No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310940.11608516501106364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288000.1075208320678791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2070650.085.093268
AGTGGTA2082750.084.830995
GTGGTAT2087950.084.556976
TGGTATC2084000.084.353257
AAGCAGT2117500.083.9502941
GTATCAA2110000.083.424319
GCAGTGG2150000.082.687783
CAGTGGT2171350.081.2656864
AGCAGTG2200550.080.8291552
CAACGCA2056350.043.95553612-13
ATCAACG2072050.043.74096710-11
ACGCAGA2069050.043.71835314-15
GAGTACT1983700.043.56098620-21
AGAGTAC2050950.043.48133518-19
TCAACGC2050400.043.00780512-13
AGTACTT2060150.042.9165120-21
AACGCAG2062700.042.8090114-15
CGCAGAG2079650.042.3962416-17
TATCAAC2092750.041.98991410-11
GTACTTT2073550.041.58282522-23
Produced by FastQC (version 0.11.5)